Origin IGS: aattgattcggtagtagaattaggacggtatatcaggcacatcaaaccaacatgccctacaccttggtatttaagccaatatcggcgactccaaatatagaaaacaaatcttttgaagttgtcattatgtcaatatttttaagactatgcaaacaaaatcacaccatactgcaaagcaggcaggacaaattgccctaccaaacacactttcgacataagataggaaaggaacccgaca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgtcgggttcctttcctatcttatgtcgaaagtgtgtttggtagggcaatttgtcctgcctgctttgcagtatggtgtgattttgtttgcatagtcttaaaaatattgacataatgacaacttcaaaagatttgttttctatatttggagtcgccgatattggcttaaataccaaggtgtagggcatgttggtttgatgtgcctgatataccgtcctaattctactaccgaatcaatt Mask Tandem Repeat Region ================================================ aattgattcggtagtagaattaggacggtatatcaggcacatcaaaccaacatgccctacaccttggtatttaagccaatatcggcgactccaaatatagaaaacaaatcttttgaagttgtcattatgtcaatatttttaagactatgcaaacaaaatcacaccatactgcaaagcaggcaggacaaattgccctaccaaacacactttcgacataagataggaaaggaacccgaca Find is-nt database================================================ Query_seq: PG1271:PG1273|PG1271:PG1273:acetylornithine aminotransferase, putative:hypothetical protein:<-<-:1351534..1351771 238 aattgattcggtagtagaattaggacggtatatcaggcacatcaaaccaacatgccctacaccttggtatttaagccaatatcggcgactccaaatatagaaaacaaatcttttgaagttgtcattatgtcaatatttttaagactatgcaaacaaaatcacaccatactgcaaagcaggcaggacaaattgccctaccaaacacactttcgacataagataggaaaggaacccgaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1271:PG1273|PG1271:PG1273:acetylornithine aminotransferase, putative:hypothetical protein:<-<-:1351534..1351771 238 aattgattcggtagtagaattaggacggtatatcaggcacatcaaaccaacatgccctacaccttggtatttaagccaatatcggcgactccaaatatagaaaacaaatcttttgaagttgtcattatgtcaatatttttaagactatgcaaacaaaatcacaccatactgcaaagcaggcaggacaaattgccctaccaaacacactttcgacataagataggaaaggaacccgaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1271:PG1273|PG1271:PG1273:acetylornithine aminotransferase, putative:hypothetical protein:<-<-:1351534..1351771 238 aattgattcggtagtagaattaggacggtatatcaggcacatcaaaccaacatgccctacaccttggtatttaagccaatatcggcgactccaaatatagaaaacaaatcttttgaagttgtcattatgtcaatatttttaagactatgcaaacaaaatcacaccatactgcaaagcaggcaggacaaattgccctaccaaacacactttcgacataagataggaaaggaacccgaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aattgattcggtagtagaattaggacggtatatcaggcacatcaaaccaacatgccctacaccttggtatttaagccaatatcggcgactccaaatatagaaaacaaatcttttgaagttgtcattatgtcaatatttttaagactatgcaaacaaaatcacaccatactgcaaagcaggcaggacaaattgccctaccaaacacactttcgacataagataggaaaggaacccgaca Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 2 End: 97 . M D S V V E L G R Y I R H I K P T C P T P W Y L S Q Y R R L Q I ............................................................................................................................................. Protein_Len: 60 Strand: + Start: 19 End: 237 .................. M R T V Y Q A H Q T N M P Y T L V F K P I S A T P N I E N K S F E V V I M S I F L R L C K Q N H T I L Q S R Q D K L P Y . Protein_Len: 31 Strand: - Start: 60 End: 152 ........................................................... V K T N L G I D A V G F I S F L D K S T T M I D I N K L S H M ...................................................................................... Protein_Len: 32 Strand: - Start: 1 End: 96 I S E T T S N P R Y I L C M L G V H G V G Q Y K L W Y R R S W M .............................................................................................................................................. aattgattcggtagtagaattaggacggtatatcaggcacatcaaaccaacatgccctacaccttggtatttaagccaatatcggcgactccaaatatagaaaacaaatcttttgaagttgtcattatgtcaatatttttaagactatgcaaacaaaatcacaccatactgcaaagcaggcaggacaaattgccctaccaaacacactttcgacataagataggaaaggaacccgaca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aattgattcggtagtagaattaggacggtatatcaggcacatcaaaccaacatgccctacaccttggtatttaagccaatatcggcgactccaaatatagaaaacaaatcttttgaagttgtcattatgtcaatatttttaagactatgcaaacaaaatcacaccatactgcaaagcaggcaggacaaattgccctaccaaacacactttcgacataagataggaaaggaacccgaca Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 75 HP_score: -9.7 Tail_Score: -2.93585 Start: 179 End: 199 Full_Region: gtcgaaagtgtgttt ggtagggca att tgtcctgcc tgctttgcagtatgg ..................................................................................................................................................................................ggtagggcaatttgtcctgcc....................................... Find igs database================================================ Query_seq: PG1271:PG1273|PG1271:PG1273:acetylornithine aminotransferase, putative:hypothetical protein:<-<-:1351534..1351771 238 aattgattcggtagtagaattaggacggtatatcaggcacatcaaaccaacatgccctacaccttggtatttaagccaatatcggcgactccaaatatagaaaacaaatcttttgaagttgtcattatgtcaatatttttaagactatgcaaacaaaatcacaccatactgcaaagcaggcaggacaaattgccctaccaaacacactttcgacataagataggaaaggaacccgaca Intra-Species Hit: Count: 1 Min: 1 Max: 238 Len: 238 Subject: NC_002950_PG1271_PG1273|acetylornithine aminotransferase, putative:hypothetical protein|NEGATIVE:NEGATIVE|[1351534,1351771]|238 HSP 1 e-value: 1.0E-132 bit: 472.0 Len: 238 Query Start:1 Query End:238 Subject Strand: POSITIVE Subject Start: 1 Subject End: 238 aattgattcggtagtagaattaggacggtatatcaggcacatcaaaccaacatgccctacaccttggtatttaagccaatatcggcgactccaaatatagaaaacaaatcttttgaagttgtcattatgtcaatatttttaagactatgcaaacaaaatcacaccatactgcaaagcaggcaggacaaattgccctaccaaacacactttcgacataagataggaaaggaacccgaca aattgattcggtagtagaattaggacggtatatcaggcacatcaaaccaacatgccctacaccttggtatttaagccaatatcggcgactccaaatatagaaaacaaatcttttgaagttgtcattatgtcaatatttttaagactatgcaaacaaaatcacaccatactgcaaagcaggcaggacaaattgccctaccaaacacactttcgacataagataggaaaggaacccgaca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUGAUUCGGUAGUAGAAUUAGGACGGUAUAUCAGGCACAUCAAACCAACAUGCCCUACACCUUGGUAUUUAAGCCAAUAUCGGCGACUCCAAAUAUAGAAAACAAAUCUUUUGAAGUUGUCAUUAUGUCAAUAUUUUUAAGACUAUGCAAACAAAAUCACACCAUACUGCAAAGCAGGCAGGACAAAUUGCCCUACCAAACACACUUUCGACAUAAGAUAGGAAAGGAACCCGACA ..(((((((.......)))))))..(((.......((((.............)))).....(((((((......)))..(((((((((((.((((...(((.......))))))).)))))))..((((((...........(.((.((((..................)))).))).(((((......))))).................)))))).))))...))))...)))... (-43.19) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUCGGGUUCCUUUCCUAUCUUAUGUCGAAAGUGUGUUUGGUAGGGCAAUUUGUCCUGCCUGCUUUGCAGUAUGGUGUGAUUUUGUUUGCAUAGUCUUAAAAAUAUUGACAUAAUGACAACUUCAAAAGAUUUGUUUUCUAUAUUUGGAGUCGCCGAUAUUGGCUUAAAUACCAAGGUGUAGGGCAUGUUGGUUUGAUGUGCCUGAUAUACCGUCCUAAUUCUACUACCGAAUCAAUU ((((.((.......))....(((((((((..........((((((((.....)))))))).(((.(((((.(..(.......)..)))))).)))..........))))))))).)))).........((((((........(((.(((...((((....))))...........(((((((((((((((.....)))))))))..)))))).))).)))........)))))).... (-62.49) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 955 70 21 161 112 NC_002950:PG1271|5end_acetylornithine_aminotransferase,_putative_1351443..1351673_NEGATIVE 301 70 21 155 99 NC_002950:PG0495|5end_hypothetical_protein_535447..535677_POSITIVE 297 216 173 91 49 NC_002950:PG1850|5end_hypothetical_protein_1949957..1950187_NEGATIVE 288 86 41 49 2 NC_002950:PG2127|5end_hypothetical_protein_2233552..2233782_NEGATIVE 282 93 52 191 146 NC_002950:PG1352|5end_hypothetical_protein_1433150..1433380_NEGATIVE 280 198 164 83 43 NC_002950:PG0172|5end_exonuclease_193493..193723_POSITIVE 279 98 51 219 166 NC_002950:PG1068|5end_hypothetical_protein_1134636..1134866_POSITIVE 279 88 53 159 103 NC_002950:PG0876|5end_tRNA_modification_GTPase_940806..941036_NEGATIVE 274 49 3 215 158 NC_002950:PG1540|5end_S-adenosylmethionine:tRNA_ribosyltransferase-isomerase_1619277..1619507_POSITIVE 274 194 156 71 37 NC_002950:PG1385|5end_TPR_domain_protein_1466335..1466565_NEGATIVE 273 196 160 66 29 NC_002950:PG1095|5end_RNA_methyltransferase,_TrmA_family_1160225..1160455_POSITIVE 271 216 176 107 44 NC_002950:PG2114|5end_hypothetical_protein_2224158..2224388_NEGATIVE 271 66 26 50 13 NC_002950:PG1593|5end_shikimate_kinase_1671503..1671733_POSITIVE 269 92 51 77 35 NC_002950:PG0403|5end_hypothetical_protein_439761..439991_NEGATIVE 267 199 153 197 148 NC_002950:PG1372|5end_hypothetical_protein_1454722..1454952_NEGATIVE 266 111 74 178 126 NC_002950:PG1389|5end_DNA-binding_protein,_histone-like_family_1471328..1471558_NEGATIVE 265 38 7 205 175 NC_002950:PG1709|5end_ISPg5,_transposase_Orf1_1794914..1795144_POSITIVE 262 86 53 226 193 NC_002950:PG1302|5end_hypothetical_protein_1380637..1380867_NEGATIVE 260 92 54 108 62 NC_002950:PG1388|5end_hypothetical_protein_1470889..1471119_NEGATIVE 259 200 159 66 29 NC_002950:PG1230|5end_hypothetical_protein_1308068..1308298_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 318 48 2 118 69 NC_002950:PG1402|3end_AP_endonuclease_domain_protein_1485031..1485181_NEGATIVE 297 216 173 94 52 NC_002950:PG1851|3end_phosphopantothenoylcysteine_decarboxylase/phosphopantothenate--cysteine_ligase_1949954..1950104_NEGATIVE 289 213 171 104 69 NC_002950:PG1761|3end_acetyltransferase,_GNAT_family_1847739..1847889_POSITIVE 286 92 51 65 16 NC_002950:PG2075|3end_hypothetical_protein_2176248..2176398_NEGATIVE 286 92 51 65 16 NC_002950:PG1671|3end_hypothetical_protein_1757440..1757590_NEGATIVE 286 92 51 65 16 NC_002950:PG1266|3end_hypothetical_protein_1346769..1346919_NEGATIVE 286 92 51 65 16 NC_002950:PG0101|3end_hypothetical_protein_119496..119646_POSITIVE 282 93 52 140 95 NC_002950:PG1353|3end_orotate_phosphoribosyltransferase_1433201..1433351_NEGATIVE 282 199 159 75 36 NC_002950:PG0164|3end_hypothetical_protein_187081..187231_NEGATIVE 280 198 164 86 46 NC_002950:PG0171|3end_5'-nucleotidase_family_protein_193576..193726_POSITIVE 279 88 53 127 71 NC_002950:PG0877|3end_hypothetical_protein_940838..940988_NEGATIVE 274 194 156 37 3 NC_002950:PG1386|3end_DNA_gyrase,_A_subunit_1466369..1466519_NEGATIVE 274 228 181 84 28 NC_002950:PG0562|3end_potassium_uptake_protein_TrkA,_putative_620978..621128_POSITIVE 271 88 48 143 95 NC_002950:PG1232|3end_glutamate_dehydrogenase_1308274..1308424_NEGATIVE 269 92 51 70 28 NC_002950:PG0404|3end_hypothetical_protein_439768..439918_NEGATIVE 260 69 21 86 40 NC_002950:PG2041|3end_hypothetical_protein_2139235..2139385_NEGATIVE 260 92 54 127 81 NC_002950:PG1389|3end_DNA-binding_protein,_histone-like_family_1470870..1471020_NEGATIVE 256 93 62 119 84 NC_002950:PG1372|3end_hypothetical_protein_1453301..1453451_NEGATIVE 254 69 24 62 24 NC_002950:PG1735|3end_MutT/nudix_family_protein_1822528..1822678_NEGATIVE 254 206 169 44 10 NC_002950:PG0439|3end_hypothetical_protein_478810..478960_POSITIVE