Origin IGS: agataaacgttacacaagtaacttgtattaaccatcctgaaacgacaggacaatcgcatttgaaatataagcctgatgatgctccgtccggaataaacacgaattaatgtgttagagatcctaatagtagaaggaagagaccaaggatacagagtacaacctttcaaatacgatagccctctgtcgaagagaccggcctcttcggggaattttctgtagtctgtgttgcgcttactatcggaaattacaatcacgggtgtctttctattataagaaatgagtcctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgtaagtgtttcgtaccggcttttcaacacttcttcgtattcctctgcataatcttcaatctcgtaccaagaggtatcacctgagagcgtagagaggaaaacgatcaggagtaaaaagttgagaggataaacttctttgttctctatccgtgtggatccgagaccatgaagagattttccgatagtgactgaatacatgaccgacgactcattcaacgactataaaacttatagaatgctgttttgaatgccttgccctgcaaacaccttccaaattatggtctgtcaatattttttctttaatttggtcttttgaaaacggaatcattatctgataattaccttccgttgtattggaaaagtaccgaattgtatctctccggacaggaggttttcggtggaagtgaacattaaagcagtaacgt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 acgttactgctttaatgttcacttccaccgaaaacctcctgtccggagagatacaattcggtacttttccaatacaacggaaggtaattatcagataatgattccgttttcaaaagaccaaattaaagaaaaaatattgacagaccataatttggaaggtgtttgcagggcaaggcattcaaaacagcattctataagttttatagtcgttgaatgagtcgtcggtcatgtattcagtcactatcggaaaatctcttcatggtctcggatccacacggatagagaacaaagaagtttatcctctcaactttttactcctgatcgttttcctctctacgctctcaggtgatacctcttggtacgagattgaagattatgcagaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgccttcctgcacggtgcaggagaccggcgtaggcctacggtgctcattgcaacatgctaattaccagtacattatggccaattaaggtgaaatgaaaataatcttggaagttttccctgttttgcgtcttcaaaattcatttttcccccttgtttgggaagatcaggactcatttcttataatagaaagacacccgtgattgtaatttccgatagtaagcgcaacacagactacagaaaattccccgaagaggccggtctcttcgacagagggctatcgtatttgaaaggttgtactctgtatccttggtctcttccttctactattaggatctctaacacattaattcgtgtttattccggacggagcatcatcaggcttatatttcaaatgcgattgtcctgtcgtttcaggatggttaatacaagttacttgtgtaacgtttatct Mask Tandem Repeat Region ================================================ agataaacgttacacaagtaacttgtattaaccatcctgaaacgacaggacaatcgcatttgaaatataagcctgatgatgctccgtccggaataaacacgaattaatgtgttagagatcctaatagtagaaggaagagaccaaggatacagagtacaacctttcaaatacgatagccctctgtcgaagagaccggcctcttcggggaattttctgtagtctgtgttgcgcttactatcggaaattacaatcacgggtgtctttctattataagaaatgagtcctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgtaagtgtttcgtaccggcttttcaacacttcttcgtattcctctgcataatcttcaatctcgtaccaagaggtatcacctgagagcgtagagaggaaaacgatcaggagtaaaaagttgagaggataaacttctttgttctctatccgtgtggatccgagaccatgaagagattttccgatagtgactgaatacatgaccgacgactcattcaacgactataaaacttatagaatgctgttttgaatgccttgccctgcaaacaccttccaaattatggtctgtcaatattttttctttaatttggtcttttgaaaacggaatcattatctgataattaccttccgttgtattggaaaagtaccgaattgtatctctccggacaggaggttttcggtggaagtgaacattaaagcagtaacgt Find is-nt database================================================ Query_seq: PG1186:PG1189|PG1186:PG1189:hypothetical protein:hypothetical protein:<-->:1269242..1270131 890 agataaacgttacacaagtaacttgtattaaccatcctgaaacgacaggacaatcgcatttgaaatataagcctgatgatgctccgtccggaataaacacgaattaatgtgttagagatcctaatagtagaaggaagagaccaaggatacagagtacaacctttcaaatacgatagccctctgtcgaagagaccggcctcttcggggaattttctgtagtctgtgttgcgcttactatcggaaattacaatcacgggtgtctttctattataagaaatgagtcctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgtaagtgtttcgtaccggcttttcaacacttcttcgtattcctctgcataatcttcaatctcgtaccaagaggtatcacctgagagcgtagagaggaaaacgatcaggagtaaaaagttgagaggataaacttctttgttctctatccgtgtggatccgagaccatgaagagattttccgatagtgactgaatacatgaccgacgactcattcaacgactataaaacttatagaatgctgttttgaatgccttgccctgcaaacaccttccaaattatggtctgtcaatattttttctttaatttggtcttttgaaaacggaatcattatctgataattaccttccgttgtattggaaaagtaccgaattgtatctctccggacaggaggttttcggtggaagtgaacattaaagcagtaacgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 485 Max: 617 Len: 133 Subject: IS|ISPg2 HSP 1 e-value: 3.0E-29 bit: 129.0 Len: 133 Query Start:485 Query End:617 Subject Strand: NEGATIVE Subject Start: 107 Subject End: 239 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cttttcaacacttcttcgtattcctctgcataatcttcaatctcgtaccaagaggtatcacctgagagcgtagagaggaaaacgatcaggagtaaaaagttgagaggataaacttctttgttctctatccgtg................................................................................................................................................................................................................................................................................. ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cttttcaattcttcttcgtattcctcggcataatcttcaatctcataccaagaggtattgcctgagagtgttgagaggaaaacgatcagcagtaaaaaatcaagaggataaatttttttacgctctatccgtg................................................................................................................................................................................................................................................................................. Find is-aa database================================================ Query_seq: PG1186:PG1189|PG1186:PG1189:hypothetical protein:hypothetical protein:<-->:1269242..1270131 890 agataaacgttacacaagtaacttgtattaaccatcctgaaacgacaggacaatcgcatttgaaatataagcctgatgatgctccgtccggaataaacacgaattaatgtgttagagatcctaatagtagaaggaagagaccaaggatacagagtacaacctttcaaatacgatagccctctgtcgaagagaccggcctcttcggggaattttctgtagtctgtgttgcgcttactatcggaaattacaatcacgggtgtctttctattataagaaatgagtcctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgtaagtgtttcgtaccggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntggatccgagaccatgaagagattttccgatagtgactgaatacatgaccgacgactcattcaacgactataaaacttatagaatgctgttttgaatgccttgccctgcaaacaccttccaaattatggtctgtcaatattttttctttaatttggtcttttgaaaacggaatcattatctgataattaccttccgttgtattggaaaagtaccgaattgtatctctccggacaggaggttttcggtggaagtgaacattaaagcagtaacgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1186:PG1189|PG1186:PG1189:hypothetical protein:hypothetical protein:<-->:1269242..1270131 890 agataaacgttacacaagtaacttgtattaaccatcctgaaacgacaggacaatcgcatttgaaatataagcctgatgatgctccgtccggaataaacacgaattaatgtgttagagatcctaatagtagaaggaagagaccaaggatacagagtacaacctttcaaatacgatagccctctgtcgaagagaccggcctcttcggggaattttctgtagtctgtgttgcgcttactatcggaaattacaatcacgggtgtctttctattataagaaatgagtcctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgtaagtgtttcgtaccggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntggatccgagaccatgaagagattttccgatagtgactgaatacatgaccgacgactcattcaacgactataaaacttatagaatgctgttttgaatgccttgccctgcaaacaccttccaaattatggtctgtcaatattttttctttaatttggtcttttgaaaacggaatcattatctgataattaccttccgttgtattggaaaagtaccgaattgtatctctccggacaggaggttttcggtggaagtgaacattaaagcagtaacgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agataaacgttacacaagtaacttgtattaaccatcctgaaacgacaggacaatcgcatttgaaatataagcctgatgatgctccgtccggaataaacacgaattaatgtgttagagatcctaatagtagaaggaagagaccaaggatacagagtacaacctttcaaatacgatagccctctgtcgaagagaccggcctcttcggggaattttctgtagtctgtgttgcgcttactatcggaaattacaatcacgggtgtctttctattataagaaatgagtcctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgtaagtgtttcgtaccggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntggatccgagaccatgaagagattttccgatagtgactgaatacatgaccgacgactcattcaacgactataaaacttatagaatgctgttttgaatgccttgccctgcaaacaccttccaaattatggtctgtcaatattttttctttaatttggtcttttgaaaacggaatcattatctgataattaccttccgttgtattggaaaagtaccgaattgtatctctccggacaggaggttttcggtggaagtgaacattaaagcagtaacgt Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 3 End: 104 .. M N V T Q V T C I N H P E T T G Q S H L K Y K P D D A P S G I N T N .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 38 Strand: + Start: 103 End: 216 ...................................................................................................... M N V L E I L I V E G R D Q G Y R V Q P F K Y D S P L S K R P A S S G N F L .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 35 Strand: + Start: 173 End: 277 ............................................................................................................................................................................ M A L C R R D R P L R G I F C S L C C A Y Y R K L Q S R V S F Y Y K K ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 33 Strand: + Start: 277 End: 375 .................................................................................................................................................................................................................................................................................... M S P D L P K Q G G K M N F E D A K Q G K L P R L F S F H L N W P ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: + Start: 314 End: 403 ......................................................................................................................................................................................................................................................................................................................... M L K T Q N R E N F Q D Y F H F T L I G H N V L V I S M L Q ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 48 Strand: + Start: 403 End: 546 .................................................................................................................................................................................................................................................................................................................................................................................................................. M S T V G L R R S P A P C R K A S Y A Y D R K C F V P A F Q H F F V F L C I I F N L V P R G I T ........................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 37 Strand: + Start: 687 End: 797 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M K L I E C C F E C L A L Q T P S K L W S V N I F S L I W S F E N G I I I ............................................................................................. Protein_Len: 33 Strand: + Start: 778 End: 876 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M K T E S L S D N Y L P L Y W K S T E L Y L S G Q E V F G G S E H .............. Protein_Len: 31 Strand: + Start: 797 End: 889 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M I I T F R C I G K V P N C I S P D R R F S V E V N I K A V T . Protein_Len: 53 Strand: - Start: 677 End: 835 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E V V I F S I S H Q K S H R A R C V G E L N H D T L I K E K I Q D K S F P I M I Q Y N G E T T N S F Y R M ....................................................... Protein_Len: 32 Strand: - Start: 545 End: 640 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ R L A Y L P F R D P T F L Q S S L S R Q E R D T H I R S W S S M .......................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 421 End: 681 .................................................................................................................................................................................................................................................................................................................................................................................................................................... Y E E A Y D E I E Y W S T D G S L T S L F V I L L L F N L P Y V E K N E I R T S G L G H L S K G I T V S Y M V S S E N M ................................................................................................................................................................................................................. Protein_Len: 46 Strand: - Start: 368 End: 505 ............................................................................................................................................................................................................................................................................................................................................................................... N A M I Y Q Y N A H Q L S C R L G V G T E Q V T C S P M T R R R D Y T N R V P K E V S R R M ................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 35 Strand: - Start: 254 End: 358 ............................................................................................................................................................................................................................................................. P H R E I I L F S D Q D E W V L P F F S N Q L R L V P F V E L I I K M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 3 End: 311 .. P L S W P Y L T C G K L Y S L G R D F L G A E E P F K R Y D T N R K S D S I V I V P T K R N Y S I L G S R G L C P P F M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 33 Strand: - Start: 2 End: 100 . I F T V C T V Q I L W G S V V P C D C K F Y L G S S A G D P I F M ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... agataaacgttacacaagtaacttgtattaaccatcctgaaacgacaggacaatcgcatttgaaatataagcctgatgatgctccgtccggaataaacacgaattaatgtgttagagatcctaatagtagaaggaagagaccaaggatacagagtacaacctttcaaatacgatagccctctgtcgaagagaccggcctcttcggggaattttctgtagtctgtgttgcgcttactatcggaaattacaatcacgggtgtctttctattataagaaatgagtcctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgtaagtgtttcgtaccggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntggatccgagaccatgaagagattttccgatagtgactgaatacatgaccgacgactcattcaacgactataaaacttatagaatgctgttttgaatgccttgccctgcaaacaccttccaaattatggtctgtcaatattttttctttaatttggtcttttgaaaacggaatcattatctgataattaccttccgttgtattggaaaagtaccgaattgtatctctccggacaggaggttttcggtggaagtgaacattaaagcagtaacgt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agataaacgttacacaagtaacttgtattaaccatcctgaaacgacaggacaatcgcatttgaaatataagcctgatgatgctccgtccggaataaacacgaattaatgtgttagagatcctaatagtagaaggaagagaccaaggatacagagtacaacctttcaaatacgatagccctctgtcgaagagaccggcctcttcggggaattttctgtagtctgtgttgcgcttactatcggaaattacaatcacgggtgtctttctattataagaaatgagtcctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgtaagtgtttcgtaccggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntggatccgagaccatgaagagattttccgatagtgactgaatacatgaccgacgactcattcaacgactataaaacttatagaatgctgttttgaatgccttgccctgcaaacaccttccaaattatggtctgtcaatattttttctttaatttggtcttttgaaaacggaatcattatctgataattaccttccgttgtattggaaaagtaccgaattgtatctctccggacaggaggttttcggtggaagtgaacattaaagcagtaacgt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 46 HP_score: -2.5 Tail_Score: -3.35001 Start: 180 End: 208 Full_Region: CAAATACGATAGCCC TCTGTCGAAGAG ACCGGC CTCTTCGGGGA ATTTTCTGTAGTCTG ...................................................................................................................................................................................TCTGTCGAAGAGACCGGCCTCTTCGGGGA.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: PG1186:PG1189|PG1186:PG1189:hypothetical protein:hypothetical protein:<-->:1269242..1270131 890 agataaacgttacacaagtaacttgtattaaccatcctgaaacgacaggacaatcgcatttgaaatataagcctgatgatgctccgtccggaataaacacgaattaatgtgttagagatcctaatagtagaaggaagagaccaaggatacagagtacaacctttcaaatacgatagccctctgtcgaagagaccggcctcttcggggaattttctgtagtctgtgttgcgcttactatcggaaattacaatcacgggtgtctttctattataagaaatgagtcctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgtaagtgtttcgtaccggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntggatccgagaccatgaagagattttccgatagtgactgaatacatgaccgacgactcattcaacgactataaaacttatagaatgctgttttgaatgccttgccctgcaaacaccttccaaattatggtctgtcaatattttttctttaatttggtcttttgaaaacggaatcattatctgataattaccttccgttgtattggaaaagtaccgaattgtatctctccggacaggaggttttcggtggaagtgaacattaaagcagtaacgt Intra-Species Hit: Count: 6 Min: 1 Max: 890 Len: 890 Subject: NC_002950_PG1186_PG1189|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[1269242,1270131]|890 HSP 1 e-value: 0.0 bit: 955.0 Len: 482 Query Start:1 Query End:482 Subject Strand: POSITIVE Subject Start: 1 Subject End: 482 agataaacgttacacaagtaacttgtattaaccatcctgaaacgacaggacaatcgcatttgaaatataagcctgatgatgctccgtccggaataaacacgaattaatgtgttagagatcctaatagtagaaggaagagaccaaggatacagagtacaacctttcaaatacgatagccctctgtcgaagagaccggcctcttcggggaattttctgtagtctgtgttgcgcttactatcggaaattacaatcacgggtgtctttctattataagaaatgagtcctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgtaagtgtttcgtacc........................................................................................................................................................................................................................................................................................................................................................................................................................ agataaacgttacacaagtaacttgtattaaccatcctgaaacgacaggacaatcgcatttgaaatataagcctgatgatgctccgtccggaataaacacgaattaatgtgttagagatcctaatagtagaaggaagagaccaaggatacagagtacaacctttcaaatacgatagccctctgtcgaagagaccggcctcttcggggaattttctgtagtctgtgttgcgcttactatcggaaattacaatcacgggtgtctttctattataagaaatgagtcctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgtaagtgtttcgtacc........................................................................................................................................................................................................................................................................................................................................................................................................................ HSP 2 e-value: 1.0E-152 bit: 541.0 Len: 273 Query Start:618 Query End:890 Subject Strand: POSITIVE Subject Start: 618 Subject End: 890 .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tggatccgagaccatgaagagattttccgatagtgactgaatacatgaccgacgactcattcaacgactataaaacttatagaatgctgttttgaatgccttgccctgcaaacaccttccaaattatggtctgtcaatattttttctttaatttggtcttttgaaaacggaatcattatctgataattaccttccgttgtattggaaaagtaccgaattgtatctctccggacaggaggttttcggtggaagtgaacattaaagcagtaacgt .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tggatccgagaccatgaagagattttccgatagtgactgaatacatgaccgacgactcattcaacgactataaaacttatagaatgctgttttgaatgccttgccctgcaaacaccttccaaattatggtctgtcaatattttttctttaatttggtcttttgaaaacggaatcattatctgataattaccttccgttgtattggaaaagtaccgaattgtatctctccggacaggaggttttcggtggaagtgaacattaaagcagtaacgt Subject: NC_002950_PG1566_PG1570|glutamyl-tRNA synthetase:rhodanese-like domain protein|NEGATIVE:NEGATIVE|[1647492,1648671]|1180 HSP 1 e-value: 0.0 bit: 700.0 Len: 437 Query Start:46 Query End:482 Subject Strand: NEGATIVE Subject Start: 519 Subject End: 955 .............................................caggacaatcgcatttgaaatataagcctgatgatgctccgtccggaataaacacgaattaatgtgttagagatcctaatagtagaaggaagagaccaaggatacagagtacaacctttcaaatacgatagccctctgtcgaagagaccggcctcttcggggaattttctgtagtctgtgttgcgcttactatcggaaattacaatcacgggtgtctttctattataagaaatgagtcctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgtaagtgtttcgtacc........................................................................................................................................................................................................................................................................................................................................................................................................................ .............................................cagggcaattgcatttgaaatataggtctgatgatgttccgtccggaataaacacgaattaatgtgttagagatcctaattgtagaaggaagagaccaaggatacggagtacaacctttcaaatacgatagccccctgccgaaggggccggtctcttcggggaattttctgtagtctgtgttgctcttactattggaaattacaatcatgggtgcctttctattataagaaatgagtcctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgtaacgagcaccgtacgcctacgccggtctcctgcaccgtgcaggagagcatcgtacgcctacgatcgtaagtgtttcgtacc........................................................................................................................................................................................................................................................................................................................................................................................................................ HSP 2 e-value: 1.0E-35 bit: 153.0 Len: 97 Query Start:622 Query End:718 Subject Strand: NEGATIVE Subject Start: 286 Subject End: 382 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tccgagaccatgaagagattttccgatagtgactgaatacatgaccgacgactcattcaacgactataaaacttatagaatgctgttttgaatgcct............................................................................................................................................................................ .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tccgagaccatgaagagattttccgatagagactgaatacatgactgacggctcattcaacgactataaaatttatagaacgctgttttgaatgcct............................................................................................................................................................................ Subject: NC_002950_PG2092_PG2094|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[2193001,2193410]|410 HSP 1 e-value: 5.0E-78 bit: 293.0 Len: 210 Query Start:70 Query End:279 Subject Strand: NEGATIVE Subject Start: 111 Subject End: 317 .....................................................................agcctgatgatgctccgtccggaataaacacgaattaatgtgttagagatcctaatagtagaaggaagagaccaaggatacagagtacaacctttcaaatacgatagccctctgtcgaagagaccggcctcttcggggaattttctgtagtctgtgttgcgcttactatcggaaattacaatcacgggtgtctttctattataagaaatg................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... .....................................................................agcctgatgatgctccgtccggaataagcacgaattaatgtgttagagatcctaattgtagaaggaagagaccaagg---cggagtacaacctttcaaatgcgatagccccctgccgaagaggccggcctcttcggggaattttctgtagtctctgttgctcttactattggaaattacaatcacgggtatctttctattataaggaatg................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_002950_PG0759_PG0762|TPR domain protein:trigger factor, putative|NEGATIVE:POSITIVE|[810876,812898]|2023 HSP 1 e-value: 1.0E-72 bit: 276.0 Len: 175 Query Start:289 Query End:463 Subject Strand: POSITIVE Subject Start: 1505 Subject End: 1679 ................................................................................................................................................................................................................................................................................................cttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacg........................................................................................................................................................................................................................................................................................................................................................................................................................................... ................................................................................................................................................................................................................................................................................................cttcccaaacaaggggaaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggctataatgtactggtaattagcatgttgtaattagtactgtaggcctacgctggtctcctgcaccgtgcaggaaggtttcgtacgcctacg........................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-19 bit: 99.6 Len: 50 Query Start:231 Query End:280 Subject Strand: POSITIVE Subject Start: 1402 Subject End: 1451 ......................................................................................................................................................................................................................................cttactatcggaaattacaatcacgggtgtctttctattataagaaatga.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. ......................................................................................................................................................................................................................................cttactatcggaaattacaatcacgggtgtctttctattataagaaatga.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. HSP 3 e-value: 1.0E-7 bit: 60.0 Len: 58 Query Start:411 Query End:468 Subject Strand: POSITIVE Subject Start: 1668 Subject End: 1725 ..........................................................................................................................................................................................................................................................................................................................................................................................................................cgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgt...................................................................................................................................................................................................................................................................................................................................................................................................................................... ..........................................................................................................................................................................................................................................................................................................................................................................................................................cgtacgcctacgctggtctcctgcatcgtgcaggagagtatcgtacgtctacgatcgt...................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_002950_PG0013_PG0016|hypothetical protein:sigma-54 dependent DNA-binding response regulator|POSITIVE:POSITIVE|[16502,17086]|585 HSP 1 e-value: 1.0E-66 bit: 256.0 Len: 205 Query Start:278 Query End:482 Subject Strand: NEGATIVE Subject Start: 140 Subject End: 344 .....................................................................................................................................................................................................................................................................................tgagtcctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgtaagtgtttcgtacc........................................................................................................................................................................................................................................................................................................................................................................................................................ .....................................................................................................................................................................................................................................................................................tgagtcctgatcttcccaaacaaggggggaaaatgaattttgaagacgcgaaattgggaaaatttccaaggtcgttctcgttttactttaattgtccataatgtactggtaattagcatgttgtaatgagcaccgtacgcctacgctggtctcctgcatcgtgcaggagtgcatcgtacgcctacgatcgtaagtgtttcgtacc........................................................................................................................................................................................................................................................................................................................................................................................................................ HSP 2 e-value: 2.0E-40 bit: 168.0 Len: 109 Query Start:65 Query End:173 Subject Strand: NEGATIVE Subject Start: 375 Subject End: 483 ................................................................atataagcctgatgatgctccgtccggaataaacacgaattaatgtgttagagatcctaatagtagaaggaagagaccaaggatacagagtacaacctttcaaatacga............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. ................................................................atataagcctgatgatgttctgtccggaataagcacgaattaatgtgttagagatcctaattgtagaaggaagagaccaaggatacggagtacgacctttcaaatacga............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Subject: NC_002950_PG1335_PG1337|hypothetical protein:umuD protein|NEGATIVE:POSITIVE|[1416985,1417952]|968 HSP 1 e-value: 1.0E-54 bit: 216.0 Len: 185 Query Start:284 Query End:468 Subject Strand: POSITIVE Subject Start: 201 Subject End: 384 ...........................................................................................................................................................................................................................................................................................ctgatcttcccaaacaagggggaaaaatgaattttgaagacgcaaaacagggaaaacttccaagattattttcatttcaccttaattggccataatgtactggtaattagcatgttgcaatgagcaccgtaggcctacgccggtctcctgcaccgtgcaggaaggcatcgtacgcctacgatcgt...................................................................................................................................................................................................................................................................................................................................................................................................................................... ...........................................................................................................................................................................................................................................................................................ctgaacttcccaaacaaggggggaaac-gaattttgaagacgcaaaacagggaaaatttctaaggtcgttctcgttttactttaattggccataatgcactgataattagcatgttgtaatgagcaccgtaggcctacgctggtctcctgcaccgtgcaggaaggcgtcgtacgcctacgatcgt...................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAUAAACGUUACACAAGUAACUUGUAUUAACCAUCCUGAAACGACAGGACAAUCGCAUUUGAAAUAUAAGCCUGAUGAUGCUCCGUCCGGAAUAAACACGAAUUAAUGUGUUAGAGAUCCUAAUAGUAGAAGGAAGAGACCAAGGAUACAGAGUACAACCUUUCAAAUACGAUAGCCCUCUGUCGAAGAGACCGGCCUCUUCGGGGAAUUUUCUGUAGUCUGUGUUGCGCUUACUAUCGGAAAUUACAAUCACGGGUGUCUUUCUAUUAUAAGAAAUGAGUCCUGAUCUUCCCAAACAAGGGGGAAAAAUGAAUUUUGAAGACGCAAAACAGGGAAAACUUCCAAGAUUAUUUUCAUUUCACCUUAAUUGGCCAUAAUGUACUGGUAAUUAGCAUGUUGCAAUGAGCACCGUAGGCCUACGCCGGUCUCCUGCACCGUGCAGGAAGGCAUCGUACGCCUACGAUCGUAAGUGUUUCGUACCGGCUUUUCAACACUUCUUCGUAUUCCUCUGCAUAAUCUUCAAUCUCGUACCAAGAGGUAUCACCUGAGAGCGUAGAGAGGAAAACGAUCAGGAGUAAAAAGUUGAGAGGAUAAACUUCUUUGUUCUCUAUCCGUGUGGAUCCGAGACCAUGAAGAGAUUUUCCGAUAGUGACUGAAUACAUGACCGACGACUCAUUCAACGACUAUAAAACUUAUAGAAUGCUGUUUUGAAUGCCUUGCCCUGCAAACACCUUCCAAAUUAUGGUCUGUCAAUAUUUUUUCUUUAAUUUGGUCUUUUGAAAACGGAAUCAUUAUCUGAUAAUUACCUUCCGUUGUAUUGGAAAAGUACCGAAUUGUAUCUCUCCGGACAGGAGGUUUUCGGUGGAAGUGAACAUUAAAGCAGUAACGU ......(((((((........((((((((.....(((((......)))))(((......))).)))))))).((((.(((.(((((((((((...((((((......)))))).(((((.(..((((..((((((((((((((((..((.((((........((((((........((((((..(((((((......)))))))(((((.((((((((((..(((...)))..)))).)))))).........((((..(((((((......))))).))..))))...))))).....))))))..........))))))(((........((((....))))..........................(((((((((.....(((...((((((((((.(((((((..(((((((.((((...(((((((((.....))))).))))..)))).))))))).(((...(((((((.(((((((((((((((((((((((((.(((((((..((..(((.((.....(((((....)))))....)))))..))..))))))).))))..)))))).....))))))))).....((((..((.((((.((((....))))..)))).)).)))).......)))...)))...))).))))...))).).))))))))))..(((((.....))))).)))))).......(((........)))...)))......))))))))).)))........)))).)).))))))))))).....((((.......)))).......)))))..)))).((((.......))))...).)))))))))))).))))))).))))....................))))))) (-248.87) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACGUUACUGCUUUAAUGUUCACUUCCACCGAAAACCUCCUGUCCGGAGAGAUACAAUUCGGUACUUUUCCAAUACAACGGAAGGUAAUUAUCAGAUAAUGAUUCCGUUUUCAAAAGACCAAAUUAAAGAAAAAAUAUUGACAGACCAUAAUUUGGAAGGUGUUUGCAGGGCAAGGCAUUCAAAACAGCAUUCUAUAAGUUUUAUAGUCGUUGAAUGAGUCGUCGGUCAUGUAUUCAGUCACUAUCGGAAAAUCUCUUCAUGGUCUCGGAUCCACACGGAUAGAGAACAAAGAAGUUUAUCCUCUCAACUUUUUACUCCUGAUCGUUUUCCUCUCUACGCUCUCAGGUGAUACCUCUUGGUACGAGAUUGAAGAUUAUGCAGAGGAAUACGAAGAAGUGUUGAAAAGCCGGUACGAAACACUUACGAUCGUAGGCGUACGAUGCCUUCCUGCACGGUGCAGGAGACCGGCGUAGGCCUACGGUGCUCAUUGCAACAUGCUAAUUACCAGUACAUUAUGGCCAAUUAAGGUGAAAUGAAAAUAAUCUUGGAAGUUUUCCCUGUUUUGCGUCUUCAAAAUUCAUUUUUCCCCCUUGUUUGGGAAGAUCAGGACUCAUUUCUUAUAAUAGAAAGACACCCGUGAUUGUAAUUUCCGAUAGUAAGCGCAACACAGACUACAGAAAAUUCCCCGAAGAGGCCGGUCUCUUCGACAGAGGGCUAUCGUAUUUGAAAGGUUGUACUCUGUAUCCUUGGUCUCUUCCUUCUACUAUUAGGAUCUCUAACACAUUAAUUCGUGUUUAUUCCGGACGGAGCAUCAUCAGGCUUAUAUUUCAAAUGCGAUUGUCCUGUCGUUUCAGGAUGGUUAAUACAAGUUACUUGUGUAACGUUUAUCU (((((((.........................(((((((((..((((((..((((((((((...((((((........))))))..(((((.(((.(((((.(((...........................................(((((.((((..((..((((((.(((((.(((((...((((..(((((.....)))))..((((((((((((((.((((.....(((((.((((...((((((..((((..((.((((..((((....)))).)))).))))))..))).)))....................((((.((((((((....((.((((.((.((((....))))))...)))))).....).))))))).))))...)))))))))..((((((.....((.(((((....))))).)).........((((((.....)))))).))))))...)))).)))..))))))).)))).))))...........((((.(.(((......))).))))).........))))).)))))))))))...))..)))).)))))...(((((((........)))))))...))).)))))))).)))))..........))).))))))).)))))).................(((..............((((((((....))))))))....(((((.(((((((((((((...((..(.(((......(((((((((((...........(((.....(((((........)))))...)))))).)))).)))).))))..))..))))))))))))).))))))))....))))).))))...(((((...))))))))))))...... (-247.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 948 90 41 181 132 NC_002950:PG1186|5end_hypothetical_protein_1269151..1269381_NEGATIVE 812 120 71 204 155 NC_002950:PG1788|5end_cysteine_peptidase,_putative_1875976..1876206_POSITIVE 567 100 51 229 181 NC_002950:PG0016|5end_sigma-54_dependent_DNA-binding_response_regulator_16947..17177_POSITIVE 548 468 422 207 163 NC_002950:PG1975|5end_hemagglutinin_protein_HagC_2063218..2063448_POSITIVE 506 470 421 181 122 NC_002950:PG1474|5end_conjugative_transposon_protein_TraO_1550816..1551046_NEGATIVE 450 449 405 55 1 NC_002950:PG0675|5end_indolepyruvate_ferredoxin_oxidoreductase,_alpha_subunit_726436..726666_NEGATIVE 444 727 681 156 108 NC_002950:PG0277|5end_ISPg2,_transposase_308856..309086_POSITIVE 433 449 401 144 96 NC_002950:PG1351|5end_hypothetical_protein_1432744..1432974_NEGATIVE 433 449 403 69 2 NC_002950:PG0672|5end_iron_compound_ABC_transporter,_ATP-binding_protein_723002..723232_POSITIVE 433 460 411 69 14 NC_002950:PG0279|5end_NADP-dependent_malic_enzyme_312954..313184_NEGATIVE 417 428 382 71 23 NC_002950:PG0674|5end_indolepyruvate_ferredoxin_oxidoreductase,_beta_subunit_724802..725032_NEGATIVE 410 490 443 159 121 NC_002950:PG1967|5end_TPR_domain_protein_2057168..2057398_POSITIVE 409 620 571 50 2 NC_002950:PG2176|5end_ISPg2,_transposase_2284345..2284575_NEGATIVE 409 620 571 50 2 NC_002950:PG1746|5end_ISPg2,_transposase_1832230..1832460_NEGATIVE 409 620 571 50 2 NC_002950:PG1350|5end_ISPg2,_transposase_1430866..1431096_POSITIVE 409 620 571 50 2 NC_002950:PG0865|5end_ISPg2,_transposase_928896..929126_NEGATIVE 409 449 406 230 162 NC_002950:PG0704|5end_phosphoglycerate_mutase_family_protein_758928..759158_POSITIVE 404 449 406 187 137 NC_002950:PG2029|5end_hypothetical_protein_2126543..2126773_POSITIVE 403 460 415 48 8 NC_002950:PG1479|5end_conjugative_transposon_protein_TraJ_1555234..1555464_NEGATIVE 403 469 422 209 169 NC_002950:PG0976|5end_phosphoribosylaminoimidazole-succinocarboxamide_synthase_1038326..1038556_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 945 460 412 133 85 NC_002950:PG1027|3end_hypothetical_protein_1089197..1089347_NEGATIVE 462 574 532 41 1 NC_002950:PG0013|3end_hypothetical_protein_16441..16591_POSITIVE 433 449 401 151 103 NC_002950:PG1352|3end_hypothetical_protein_1432737..1432887_NEGATIVE 403 90 43 71 10 NC_002950:PG0879|3end_hypothetical_protein_941931..942081_NEGATIVE 399 460 411 100 29 NC_002950:PG0605|3end_hypothetical_protein_661165..661315_NEGATIVE 397 459 411 150 102 NC_002950:PG0361|3end_hypothetical_protein_395481..395631_POSITIVE 395 490 442 63 15 NC_002950:PG1290|3end_branched-chain_amino_acid_aminotransferase_1369352..1369502_POSITIVE 392 99 51 82 19 NC_002950:PG0499|3end_hypothetical_protein_539142..539292_POSITIVE 384 456 411 151 103 NC_002950:PG0975|3end_PhoH_family_protein_1038356..1038506_POSITIVE 381 450 409 139 97 NC_002950:PG1389|3end_DNA-binding_protein,_histone-like_family_1470870..1471020_NEGATIVE 380 428 391 44 1 NC_002950:PG0675|3end_indolepyruvate_ferredoxin_oxidoreductase,_alpha_subunit_724829..724979_NEGATIVE 380 728 682 61 13 NC_002950:PG0428|3end_hypothetical_protein_465753..465903_NEGATIVE 378 90 41 150 94 NC_002950:PG1190|3end_glycerate_dehydrogenase_1273145..1273295_POSITIVE 375 449 407 62 19 NC_002950:PG0752|3end_uracil_phosphoribosyltransferase_798397..798547_POSITIVE 375 440 391 144 95 NC_002950:PG0259|3end_hypothetical_protein_293701..293851_POSITIVE 372 435 392 67 15 NC_002950:PG0375|3end_50S_ribosomal_protein_L13_406469..406619_POSITIVE 370 460 411 83 34 NC_002950:PG1112|3end_hypothetical_protein_1185571..1185721_POSITIVE 369 440 395 139 86 NC_002950:PG2020|3end_CRISPR-associated_TM1814_family_protein_2111608..2111758_NEGATIVE 367 460 411 136 89 NC_002950:PG2060|3end_thymidylate_synthase_2158456..2158606_POSITIVE 366 449 410 132 96 NC_002950:PG1750|3end_alpha-1,3/4-fucosidase,_putative_1837223..1837373_POSITIVE