Origin IGS: ggttcgaaagaaaaaacgcgctttgcgactctttcatattcggccgactgtgccgtctatgcttgagtcgcaaagcgcgttttatatcctgccctctccgtttgcttggc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gccaagcaaacggagagggcaggatataaaacgcgctttgcgactcaagcatagacggcacagtcggccgaatatgaaagagtcgcaaagcgcgttttttctttcgaacc Mask Tandem Repeat Region ================================================ ggttcgaaagaaaaaacgcgctttgcgactctttcatattcggccgactgtgccgtctatgcttgagtcgcaaagcgcgttttatatcctgccctctccgtttgcttggc Find is-nt database================================================ Query_seq: PG1173:PG1174|PG1173:PG1174:YkgG family protein:thioesterase family protein:-><-:1253190..1253299 110 ggttcgaaagaaaaaacgcgctttgcgactctttcatattcggccgactgtgccgtctatgcttgagtcgcaaagcgcgttttatatcctgccctctccgtttgcttggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1173:PG1174|PG1173:PG1174:YkgG family protein:thioesterase family protein:-><-:1253190..1253299 110 ggttcgaaagaaaaaacgcgctttgcgactctttcatattcggccgactgtgccgtctatgcttgagtcgcaaagcgcgttttatatcctgccctctccgtttgcttggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1173:PG1174|PG1173:PG1174:YkgG family protein:thioesterase family protein:-><-:1253190..1253299 110 ggttcgaaagaaaaaacgcgctttgcgactctttcatattcggccgactgtgccgtctatgcttgagtcgcaaagcgcgttttatatcctgccctctccgtttgcttggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggttcgaaagaaaaaacgcgctttgcgactctttcatattcggccgactgtgccgtctatgcttgagtcgcaaagcgcgttttatatcctgccctctccgtttgcttggc Predict ORF larger than 30AA ================================================ ggttcgaaagaaaaaacgcgctttgcgactctttcatattcggccgactgtgccgtctatgcttgagtcgcaaagcgcgttttatatcctgccctctccgtttgcttggc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggttcgaaagaaaaaacgcgctttgcgactctttcatattcggccgactgtgccgtctatgcttgagtcgcaaagcgcgttttatatcctgccctctccgtttgcttggc Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 91 HP_score: -14.6 Tail_Score: -2.97131 Start: 19 End: 77 Full_Region: gcaggatataaaacg cgctttgcgactcaagcatagacggc acagtcg gccgaatatgaaagagtcgcaaagcg cgttttttctttcga ..................cgctttgcgactcaagcatagacggcacagtcggccgaatatgaaagagtcgcaaagcg................................. Find igs database================================================ Query_seq: PG1173:PG1174|PG1173:PG1174:YkgG family protein:thioesterase family protein:-><-:1253190..1253299 110 ggttcgaaagaaaaaacgcgctttgcgactctttcatattcggccgactgtgccgtctatgcttgagtcgcaaagcgcgttttatatcctgccctctccgtttgcttggc Intra-Species Hit: Count: 1 Min: 1 Max: 110 Len: 110 Subject: NC_002950_PG1173_PG1174|YkgG family protein:thioesterase family protein|POSITIVE:NEGATIVE|[1253190,1253299]|110 HSP 1 e-value: 3.0E-56 bit: 218.0 Len: 110 Query Start:1 Query End:110 Subject Strand: POSITIVE Subject Start: 1 Subject End: 110 ggttcgaaagaaaaaacgcgctttgcgactctttcatattcggccgactgtgccgtctatgcttgagtcgcaaagcgcgttttatatcctgccctctccgtttgcttggc ggttcgaaagaaaaaacgcgctttgcgactctttcatattcggccgactgtgccgtctatgcttgagtcgcaaagcgcgttttatatcctgccctctccgtttgcttggc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUUCGAAAGAAAAAACGCGCUUUGCGACUCUUUCAUAUUCGGCCGACUGUGCCGUCUAUGCUUGAGUCGCAAAGCGCGUUUUAUAUCCUGCCCUCUCCGUUUGCUUGGC (((((....)).(((((((((((((((((((...((((..(((((....).))))..))))...))))))))))))))))))).......)))....(((......))). (-49.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCCAAGCAAACGGAGAGGGCAGGAUAUAAAACGCGCUUUGCGACUCAAGCAUAGACGGCACAGUCGGCCGAAUAUGAAAGAGUCGCAAAGCGCGUUUUUUCUUUCGAACC (((...(.......)..))).(((...(((((((((((((((((((...((((..((((.......))))..))))...)))))))))))))))))))....)))..... (-50.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 417 89 42 62 13 NC_002950:PG0160|5end_hypothetical_protein_184085..184315_NEGATIVE 406 97 51 72 22 NC_002950:PG0075|5end_phosphoribosylformylglycinamidine_cyclo-ligase,_putative_90438..90668_NEGATIVE 386 90 41 64 10 NC_002950:PG0321|5end_arginine/ornithine_transport_system_ATPase_354648..354878_POSITIVE 382 69 21 73 10 NC_002950:PG1343|5end_lipoate-protein_ligase_B_1423628..1423858_POSITIVE 382 81 41 54 16 NC_002950:PG0736|5end_ribonuclease_HII_785608..785838_POSITIVE 379 86 41 100 40 NC_002950:PG0802|5end_alpha_keto_acid_dehydrogenase_complex,_E3_component,_lipoamide_dehydrogenase_859722..859952_NEGATIVE 365 80 31 52 9 NC_002950:PG0738|5end_cytidine/deoxycytidylate_deaminase_family_protein_786463..786693_POSITIVE 361 90 41 84 32 NC_002950:PG1091|5end_DHH_subfamily_1_protein_1157017..1157247_POSITIVE 357 109 61 70 21 NC_002950:PG2052|5end_dihydrodipicolinate_synthase_2149636..2149866_POSITIVE 352 90 41 63 4 NC_002950:PG1925|5end_ribosomal_protein_S14_2018675..2018905_NEGATIVE 348 80 32 200 147 NC_002950:PG2126|5end_conserved_hypothetical_protein_TIGR00044_2233112..2233342_NEGATIVE 347 97 56 148 114 NC_002950:PG0562|5end_potassium_uptake_protein_TrkA,_putative_619222..619452_POSITIVE 344 77 36 118 54 NC_002950:PG1640|5end_DNA-damage-inducible_protein_F_1721245..1721475_NEGATIVE 344 110 61 186 113 NC_002950:PG0021|5end_TIM-barrel_protein,_putative,_NifR3_family_25123..25353_NEGATIVE 343 81 41 226 184 NC_002950:PG2029|5end_hypothetical_protein_2126543..2126773_POSITIVE 343 110 62 66 15 NC_002950:PG0746|5end_sensor_histidine_kinase_793903..794133_NEGATIVE 343 95 51 174 121 NC_002950:PG0538|5end_outer_membrane_efflux_protein_589142..589372_POSITIVE 341 99 52 120 65 NC_002950:PG0896|5end_beta-galactosidase_957249..957479_POSITIVE 339 55 16 147 102 NC_002950:PG2027|5end_hypothetical_protein_2125494..2125724_POSITIVE 339 100 52 218 148 NC_002950:PG1578|5end_quinolinate_synthetase_1655466..1655696_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 492 55 13 50 8 NC_002950:PG1173|3end_YkgG_family_protein_1253129..1253279_POSITIVE 406 97 51 118 68 NC_002950:PG0076|3end_N-acetylmuramoyl-L-alanine_amidase,_family_4_90392..90542_NEGATIVE 382 81 41 43 5 NC_002950:PG0735|3end_aminotransferase,_class_V_785677..785827_POSITIVE 379 86 41 95 35 NC_002950:PG0803|3end_glucosamine-6-phosphate_deaminase_859727..859877_NEGATIVE 365 80 31 55 12 NC_002950:PG0737|3end_hypothetical_protein_786546..786696_POSITIVE 352 90 41 61 2 NC_002950:PG1926|3end_50S_ribosomal_protein_L5_2018677..2018827_NEGATIVE 351 100 51 124 85 NC_002950:PG0412|3end_DNA_mismatch_repair_protein_MutL_449584..449734_NEGATIVE 345 79 39 129 100 NC_002950:PG1648|3end_RelA/SpoT_family_protein_1730082..1730232_POSITIVE 345 70 22 123 77 NC_002950:PG1510|3end_hypothetical_protein_1587429..1587579_NEGATIVE 344 77 36 103 39 NC_002950:PG1641|3end_phosphotyrosine_protein_phosphatase_1721260..1721410_NEGATIVE 344 81 43 147 100 NC_002950:PG0443|3end_hemagglutinin-related_protein_483147..483297_POSITIVE 343 110 62 69 18 NC_002950:PG0747|3end_sigma-54_dependent_DNA-binding_response_regulator_793900..794050_NEGATIVE 339 55 16 118 73 NC_002950:PG2026|3end_phosphoglycerate_mutase_family_protein_2125545..2125695_POSITIVE 339 80 41 64 9 NC_002950:PG1342|3end_UDP-N-acetylenolpyruvoylglucosamine_reductase_1423681..1423831_POSITIVE 338 57 17 138 99 NC_002950:PG1675|3end_hypothetical_protein_1760360..1760510_POSITIVE 337 85 42 145 100 NC_002950:PG2020|3end_CRISPR-associated_TM1814_family_protein_2111608..2111758_NEGATIVE 336 70 23 139 95 NC_002950:PG1625|3end_hypothetical_protein_1708718..1708868_POSITIVE 334 90 45 118 88 NC_002950:PG0021|3end_TIM-barrel_protein,_putative,_NifR3_family_24122..24272_NEGATIVE 330 110 64 118 73 NC_002950:PG1494|3end_hypothetical_protein_1571234..1571384_POSITIVE 330 58 17 86 43 NC_002950:PG1162|3end_ATP:cob(I)alamin_adenosyltransferase,_putative_1241473..1241623_NEGATIVE