Origin IGS: acttctcgacagatctgtgattaaactcacaacaaaaaagtgaatccgattgttataaggacggttgccaaactcagcactgattgctattatggtgataccgtgactcaaaacattaaaaacaataagtaaagaacaatgagaaagattgtagttacagccctcatgggtattggtctcttggtaggcacgacggtaaatgctcaaacgtaaaagaaagtagaaactgctaagacagaacaaacggtaaagaaacaagagtgcacccaccaccacgacaaagcttgtgatgccaagaaagctggcgagagttgcaaagacaagg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ccttgtctttgcaactctcgccagctttcttggcatcacaagctttgtcgtggtggtgggtgcactcttgtttctttaccgtttgttctgtcttagcagtttctactttcttttacgtttgagcatttaccgtcgtgcctaccaagagaccaatacccatgagggctgtaactacaatctttctcattgttctttacttattgtttttaatgttttgagtcacggtatcaccataatagcaatcagtgctgagtttggcaaccgtccttataacaatcggattcacttttttgttgtgagtttaatcacagatctgtcgagaagt Mask Tandem Repeat Region ================================================ acttctcgacagatctgtgattaaactcacaacaaaaaagtgaatccgattgttataaggacggttgccaaactcagcactgattgctattatggtgataccgtgactcaaaacattaaaaacaataagtaaagaacaatgagaaagattgtagttacagccctcatgggtattggtctcttggtaggcacgacggtaaatgctcaaacgtaaaagaaagtagaaactgctaagacagaacaaacggtaaagaaacaagagtgcacccaccaccacgacaaagcttgtgatgccaagaaagctggcgagagttgcaaagacaagg Find is-nt database================================================ Query_seq: PG1151:PG1152|PG1151:PG1152:alcohol dehydrogenase, iron-containing:hypothetical protein:-><-:1234439..1234763 325 acttctcgacagatctgtgattaaactcacaacaaaaaagtgaatccgattgttataaggacggttgccaaactcagcactgattgctattatggtgataccgtgactcaaaacattaaaaacaataagtaaagaacaatgagaaagattgtagttacagccctcatgggtattggtctcttggtaggcacgacggtaaatgctcaaacgtaaaagaaagtagaaactgctaagacagaacaaacggtaaagaaacaagagtgcacccaccaccacgacaaagcttgtgatgccaagaaagctggcgagagttgcaaagacaagg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1151:PG1152|PG1151:PG1152:alcohol dehydrogenase, iron-containing:hypothetical protein:-><-:1234439..1234763 325 acttctcgacagatctgtgattaaactcacaacaaaaaagtgaatccgattgttataaggacggttgccaaactcagcactgattgctattatggtgataccgtgactcaaaacattaaaaacaataagtaaagaacaatgagaaagattgtagttacagccctcatgggtattggtctcttggtaggcacgacggtaaatgctcaaacgtaaaagaaagtagaaactgctaagacagaacaaacggtaaagaaacaagagtgcacccaccaccacgacaaagcttgtgatgccaagaaagctggcgagagttgcaaagacaagg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1151:PG1152|PG1151:PG1152:alcohol dehydrogenase, iron-containing:hypothetical protein:-><-:1234439..1234763 325 acttctcgacagatctgtgattaaactcacaacaaaaaagtgaatccgattgttataaggacggttgccaaactcagcactgattgctattatggtgataccgtgactcaaaacattaaaaacaataagtaaagaacaatgagaaagattgtagttacagccctcatgggtattggtctcttggtaggcacgacggtaaatgctcaaacgtaaaagaaagtagaaactgctaagacagaacaaacggtaaagaaacaagagtgcacccaccaccacgacaaagcttgtgatgccaagaaagctggcgagagttgcaaagacaagg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acttctcgacagatctgtgattaaactcacaacaaaaaagtgaatccgattgttataaggacggttgccaaactcagcactgattgctattatggtgataccgtgactcaaaacattaaaaacaataagtaaagaacaatgagaaagattgtagttacagccctcatgggtattggtctcttggtaggcacgacggtaaatgctcaaacgtaaaagaaagtagaaactgctaagacagaacaaacggtaaagaaacaagagtgcacccaccaccacgacaaagcttgtgatgccaagaaagctggcgagagttgcaaagacaagg Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 40 End: 129 ....................................... M N P I V I R T V A K L S T D C Y Y G D T V T Q N I K N N K .................................................................................................................................................................................................... Protein_Len: 39 Strand: - Start: 207 End: 323 .............................................................................................................................................................................................................. V Y F F T S V A L V S C V T F F C S H V W W W S L A Q S A L F A P S L Q L S M .. Protein_Len: 60 Strand: - Start: 1 End: 291 V N F V I L L S C H S L N Y N C G E H T N T E Q Y A R R Y I S L R L L F Y F S S L C F L R Y L F L L A G V V V V F S T M .................................. acttctcgacagatctgtgattaaactcacaacaaaaaagtgaatccgattgttataaggacggttgccaaactcagcactgattgctattatggtgataccgtgactcaaaacattaaaaacaataagtaaagaacaatgagaaagattgtagttacagccctcatgggtattggtctcttggtaggcacgacggtaaatgctcaaacgtaaaagaaagtagaaactgctaagacagaacaaacggtaaagaaacaagagtgcacccaccaccacgacaaagcttgtgatgccaagaaagctggcgagagttgcaaagacaagg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 88 Start: 64 End: 93 ...............................................................GTTGCCAAACTCAGCACTGATTGCTATTAT........................................................................................................................................................................................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acttctcgacagatctgtgattaaactcacaacaaaaaagtgaatccgattgttataaggacggttgccaaactcagcactgattgctattatggtgataccgtgactcaaaacattaaaaacaataagtaaagaacaatgagaaagattgtagttacagccctcatgggtattggtctcttggtaggcacgacggtaaatgctcaaacgtaaaagaaagtagaaactgctaagacagaacaaacggtaaagaaacaagagtgcacccaccaccacgacaaagcttgtgatgccaagaaagctggcgagagttgcaaagacaagg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG1151:PG1152|PG1151:PG1152:alcohol dehydrogenase, iron-containing:hypothetical protein:-><-:1234439..1234763 325 acttctcgacagatctgtgattaaactcacaacaaaaaagtgaatccgattgttataaggacggttgccaaactcagcactgattgctattatggtgataccgtgactcaaaacattaaaaacaataagtaaagaacaatgagaaagattgtagttacagccctcatgggtattggtctcttggtaggcacgacggtaaatgctcaaacgtaaaagaaagtagaaactgctaagacagaacaaacggtaaagaaacaagagtgcacccaccaccacgacaaagcttgtgatgccaagaaagctggcgagagttgcaaagacaagg Intra-Species Hit: Count: 1 Min: 1 Max: 325 Len: 325 Subject: NC_002950_PG1151_PG1152|alcohol dehydrogenase, iron-containing:hypothetical protein|POSITIVE:NEGATIVE|[1234439,1234763]|325 HSP 1 e-value: 0.0 bit: 644.0 Len: 325 Query Start:1 Query End:325 Subject Strand: POSITIVE Subject Start: 1 Subject End: 325 acttctcgacagatctgtgattaaactcacaacaaaaaagtgaatccgattgttataaggacggttgccaaactcagcactgattgctattatggtgataccgtgactcaaaacattaaaaacaataagtaaagaacaatgagaaagattgtagttacagccctcatgggtattggtctcttggtaggcacgacggtaaatgctcaaacgtaaaagaaagtagaaactgctaagacagaacaaacggtaaagaaacaagagtgcacccaccaccacgacaaagcttgtgatgccaagaaagctggcgagagttgcaaagacaagg acttctcgacagatctgtgattaaactcacaacaaaaaagtgaatccgattgttataaggacggttgccaaactcagcactgattgctattatggtgataccgtgactcaaaacattaaaaacaataagtaaagaacaatgagaaagattgtagttacagccctcatgggtattggtctcttggtaggcacgacggtaaatgctcaaacgtaaaagaaagtagaaactgctaagacagaacaaacggtaaagaaacaagagtgcacccaccaccacgacaaagcttgtgatgccaagaaagctggcgagagttgcaaagacaagg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACUUCUCGACAGAUCUGUGAUUAAACUCACAACAAAAAAGUGAAUCCGAUUGUUAUAAGGACGGUUGCCAAACUCAGCACUGAUUGCUAUUAUGGUGAUACCGUGACUCAAAACAUUAAAAACAAUAAGUAAAGAACAAUGAGAAAGAUUGUAGUUACAGCCCUCAUGGGUAUUGGUCUCUUGGUAGGCACGACGGUAAAUGCUCAAACGUAAAAGAAAGUAGAAACUGCUAAGACAGAACAAACGGUAAAGAAACAAGAGUGCACCCACCACCACGACAAAGCUUGUGAUGCCAAGAAAGCUGGCGAGAGUUGCAAAGACAAGG ..((((((.(((.((((((.....((((...((......))....(((.(((((....(..((.(((((......((((.....))))......(((.(((((.(((.(((.................((((...(((((.......)))))..)))).((((....)))).))))))...)))))..)))...))))).))..).............(((((...))))).......))))).)))...........)))).....)))...(((((......)))))......)))...))).)))))).............. (-65.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CCUUGUCUUUGCAACUCUCGCCAGCUUUCUUGGCAUCACAAGCUUUGUCGUGGUGGUGGGUGCACUCUUGUUUCUUUACCGUUUGUUCUGUCUUAGCAGUUUCUACUUUCUUUUACGUUUGAGCAUUUACCGUCGUGCCUACCAAGAGACCAAUACCCAUGAGGGCUGUAACUACAAUCUUUCUCAUUGUUCUUUACUUAUUGUUUUUAAUGUUUUGAGUCACGGUAUCACCAUAAUAGCAAUCAGUGCUGAGUUUGGCAACCGUCCUUAUAACAAUCGGAUUCACUUUUUUGUUGUGAGUUUAAUCACAGAUCUGUCGAGAAGU ..((((..(((((.((((((((((.....)))))............(((....(((((((..(((...............((..(..((((....))))..)..))..........((....)).......)).)..)))))))...)))..........))))).)))))..))))..((((((.........................((((.((((..(((((....(((...(((((.....)))))....)))..))))).)))).))))..((((((...........(((((......)))))))))))..)))))). (-72.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 399 197 155 186 128 NC_002950:PG0230|5end_transaldolase_265372..265602_POSITIVE 349 308 262 72 32 NC_002950:PG2227|5end_hypothetical_protein_2342008..2342238_POSITIVE 348 196 153 131 83 NC_002950:PG0463|5end_folylpolyglutamate_synthase_507606..507836_NEGATIVE 340 309 263 55 6 NC_002950:PG1576|5end_L-aspartate_oxidase_1653016..1653246_POSITIVE 340 317 276 78 37 NC_002950:PG0708|5end_peptidyl-prolyl_cis-trans_isomerase,_FKBP-type_764628..764858_NEGATIVE 337 316 287 47 19 NC_002950:PG0676|5end_oxidoreductase,_short_chain_dehydrogenase/reductase_family_727275..727505_NEGATIVE 334 198 151 195 134 NC_002950:PG2205|5end_2-dehydropantoate_2-reductase_2316121..2316351_POSITIVE 334 196 154 207 156 NC_002950:PG2112|5end_hypothetical_protein_2221471..2221701_POSITIVE 334 308 261 70 21 NC_002950:PG1230|5end_hypothetical_protein_1308068..1308298_NEGATIVE 329 308 261 123 71 NC_002950:PG1091|5end_DHH_subfamily_1_protein_1157017..1157247_POSITIVE 327 309 262 57 14 NC_002950:PG0714|5end_copper_homeostasis_protein_CutC_768671..768901_NEGATIVE 324 309 270 37 1 NC_002950:PG0255|5end_translation_initiation_factor_IF-2_286542..286772_POSITIVE 323 315 273 83 34 NC_002950:PG1956|5end_4-hydroxybutyrate_CoA-transferase_2047510..2047740_NEGATIVE 322 187 141 71 12 NC_002950:PG1983|5end_CRISPR-associated_TM1791_family_protein_2073442..2073672_NEGATIVE 320 210 162 218 163 NC_002950:PG0732|5end_hypothetical_protein_782621..782851_POSITIVE 319 309 261 63 9 NC_002950:PG1008|5end_hypothetical_protein_1067644..1067874_NEGATIVE 316 320 279 62 23 NC_002950:PG1774|5end_transcription-repair_coupling_factor_1864547..1864777_NEGATIVE 313 194 160 47 8 NC_002950:PG2131|5end_60_kDa_protein_2236174..2236404_POSITIVE 309 196 158 207 162 NC_002950:PG2110|5end_thiamine_biosynthesis_protein_ThiC_2221212..2221442_NEGATIVE 308 210 162 207 153 NC_002950:PG1342|5end_UDP-N-acetylenolpyruvoylglucosamine_reductase_1422585..1422815_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 399 197 155 84 26 NC_002950:PG0229|3end_hypothetical_protein_265350..265500_POSITIVE 349 308 262 75 35 NC_002950:PG2226|3end_hypothetical_protein_2342091..2342241_POSITIVE 348 196 153 134 86 NC_002950:PG0464|3end_adenylosuccinate_synthetase_507603..507753_NEGATIVE 340 317 276 50 9 NC_002950:PG0709|3end_peptidyl-prolyl_cis-trans_isomerase_FkpA,_FKBP-type_764656..764806_NEGATIVE 333 294 257 54 13 NC_002950:PG1866|3end_hypothetical_protein_1964736..1964886_POSITIVE 332 205 166 135 97 NC_002950:PG1758|3end_ribosomal_protein_S15_1845534..1845684_POSITIVE 327 309 262 52 9 NC_002950:PG0715|3end_transporter_768676..768826_NEGATIVE 322 187 141 74 15 NC_002950:PG1984|3end_hypothetical_protein_2073439..2073589_NEGATIVE 319 309 261 66 12 NC_002950:PG1009|3end_hypothetical_protein_1067641..1067791_NEGATIVE 312 98 53 83 34 NC_002950:PG0867|3end_hypothetical_protein_930238..930388_NEGATIVE 309 196 158 137 92 NC_002950:PG2111|3end_thiS_protein_2221282..2221432_NEGATIVE 303 197 151 79 12 NC_002950:PG0128|3end_hypothetical_protein_150648..150798_POSITIVE 299 315 273 91 47 NC_002950:PG0085|3end_alpha-galactosidase_100556..100706_POSITIVE 293 200 154 114 68 NC_002950:PG2128|3end_ISPg5,_transposase_Orf2_2233867..2234017_NEGATIVE 293 293 257 104 60 NC_002950:PG0875|3end_mobilizable_transposon,_tnpA_protein_938102..938252_NEGATIVE 293 200 154 114 68 NC_002950:PG0591|3end_ISPg5,_transposase_Orf2_649502..649652_POSITIVE 293 200 154 114 68 NC_002950:PG0458|3end_ISPg5,_transposase_Orf2_500525..500675_NEGATIVE 293 200 154 114 68 NC_002950:PG0041|3end_ISPg5_transposase_Orf2_49395..49545_POSITIVE 293 200 154 114 68 NC_002950:PG0008|3end_ISPg5_transposase_Orf2_7085..7235_NEGATIVE 291 205 161 127 72 NC_002950:PG1174|3end_thioesterase_family_protein_1253210..1253360_NEGATIVE