Origin IGS: catgaaatcgatattttgtcggcatctgttttattgccgccgtttccgagcacaaaggtagtcttatttggcaactgaccgtcaactaagaaaaaagataatatacttgttgtacagcacaaaccttatgcttatcaagttactttttgtatgattacgtttgtataattacacatagagagatttgtatacgtcaataaaataagctaagctttgaaagctcaatcaatttaaatagtccacattaacaactaatttgaacaaaaaaggtgtcctaaaaatttttgctacctttgcaaggtctgataaggatctcttccatagagccgagagatgcataggccaaagcaagcgtttcttcttgctggacacaagtaaatagaagatatattgtactgataatgattggacgattaaagacttcggcttttgcttttaatttacacttaaaagaaagcccaacgccccaactctcctgctttacgcctgataatgatccgatatgccacaaggatttcggacaaaggggcttactatgctatgacgagtagagtggaataagacttcgacaccacctaaaatttcataaggcacccgcttgatttgtaggcgaatcaggcgggtgcctcttttatttcggctctcgaaaaagcctttttcggcctgcgatttatatacaaatcactttcaagctatatataaatggaaaacgatctgtatatttattgcaaatgatttatatataaatcgtttccagatctcttttagatcaaaactcaaactgaaaaagtccgttttctcatttcttcgacttcaaaatccgcttccattcaggtgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggctcgtatttgggtattgcgaaaacataaaggagaattgcaagccaaagagataaaaaaggc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gccttttttatctctttggcttgcaattctcctttatgttttcgcaatacccaaatacgagccaaaaaagttttcaacaacgaagacgagtttggaatgattccaaataagcacctgaatggaagcggattttgaagtcgaagaaatgagaaaacggactttttcagtttgagttttgatctaaaagagatctggaaacgatttatatataaatcatttgcaataaatatacagatcgttttccatttatatatagcttgaaagtgatttgtatataaatcgcaggccgaaaaaggctttttcgagagccgaaataaaagaggcacccgcctgattcgcctacaaatcaagcgggtgccttatgaaattttaggtggtgtcgaagtcttattccactctactcgtcatagcatagtaagcccctttgtccgaaatccttgtggcatatcggatcattatcaggcgtaaagcaggagagttggggcgttgggctttcttttaagtgtaaattaaaagcaaaagccgaagtctttaatcgtccaatcattatcagtacaatatatcttctatttacttgtgtccagcaagaagaaacgcttgctttggcctatgcatctctcggctctatggaagagatccttatcagaccttgcaaaggtagcaaaaatttttaggacaccttttttgttcaaattagttgttaatgtggactatttaaattgattgagctttcaaagcttagcttattttattgacgtatacaaatctctctatgtgtaattatacaaacgtaatcatacaaaaagtaacttgataagcataaggtttgtgctgtacaacaagtatattatcttttttcttagttgacggtcagttgccaaataagactacctttgtgctcggaaacggcggcaataaaacagatgccgacaaaatatcgatttcatg Mask Tandem Repeat Region ================================================ catgaaatcgatattttgtcggcatctgttttattgccgccgtttccgagcacaaaggtagtcttatttggcaactgaccgtcaactaagaaaaaagataatatacttgttgtacagcacaaaccttatgcttatcaagttactttttgtatgattacgtttgtataattacacatagagagatttgtatacgtcaataaaataagctaagctttgaaagctcaatcaatttaaatagtccacattaacaactaatttgaacaaaaaaggtgtcctaaaaatttttgctacctttgcaaggtctgataaggatctcttccatagagccgagagatgcataggccaaagcaagcgtttcttcttgctggacacaagtaaatagaagatatattgtactgataatgattggacgattaaagacttcggcttttgcttttaatttacacttaaaagaaagcccaacgccccaactctcctgctttacgcctgataatgatccgatatgccacaaggatttcggacaaaggggcttactatgctatgacgagtagagtggaataagacttcgacaccacctaaaatttcataaggcacccgcttgatttgtaggcgaatcaggcgggtgcctcttttatttcggctctcgaaaaagcctttttcggcctgcgatttatatacaaatcactttcaagctatatataaatggaaaacgatctgtatatttattgcaaatgatttatatataaatcgtttccagatctcttttagatcaaaactcaaactgaaaaagtccgttttctcatttcttcgacttcaaaatccgcttccattcaggtgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggctcgtatttgggtattgcgaaaacataaaggagaattgcaagccaaagagataaaaaaggc Find is-nt database================================================ Query_seq: PG1145:PG1148|PG1145:PG1148:long-chain-fatty-acid--CoA ligase, putative:hypothetical protein:<-->:1230396..1231343 948 catgaaatcgatattttgtcggcatctgttttattgccgccgtttccgagcacaaaggtagtcttatttggcaactgaccgtcaactaagaaaaaagataatatacttgttgtacagcacaaaccttatgcttatcaagttactttttgtatgattacgtttgtataattacacatagagagatttgtatacgtcaataaaataagctaagctttgaaagctcaatcaatttaaatagtccacattaacaactaatttgaacaaaaaaggtgtcctaaaaatttttgctacctttgcaaggtctgataaggatctcttccatagagccgagagatgcataggccaaagcaagcgtttcttcttgctggacacaagtaaatagaagatatattgtactgataatgattggacgattaaagacttcggcttttgcttttaatttacacttaaaagaaagcccaacgccccaactctcctgctttacgcctgataatgatccgatatgccacaaggatttcggacaaaggggcttactatgctatgacgagtagagtggaataagacttcgacaccacctaaaatttcataaggcacccgcttgatttgtaggcgaatcaggcgggtgcctcttttatttcggctctcgaaaaagcctttttcggcctgcgatttatatacaaatcactttcaagctatatataaatggaaaacgatctgtatatttattgcaaatgatttatatataaatcgtttccagatctcttttagatcaaaactcaaactgaaaaagtccgttttctcatttcttcgacttcaaaatccgcttccattcaggtgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggctcgtatttgggtattgcgaaaacataaaggagaattgcaagccaaagagataaaaaaggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1145:PG1148|PG1145:PG1148:long-chain-fatty-acid--CoA ligase, putative:hypothetical protein:<-->:1230396..1231343 948 catgaaatcgatattttgtcggcatctgttttattgccgccgtttccgagcacaaaggtagtcttatttggcaactgaccgtcaactaagaaaaaagataatatacttgttgtacagcacaaaccttatgcttatcaagttactttttgtatgattacgtttgtataattacacatagagagatttgtatacgtcaataaaataagctaagctttgaaagctcaatcaatttaaatagtccacattaacaactaatttgaacaaaaaaggtgtcctaaaaatttttgctacctttgcaaggtctgataaggatctcttccatagagccgagagatgcataggccaaagcaagcgtttcttcttgctggacacaagtaaatagaagatatattgtactgataatgattggacgattaaagacttcggcttttgcttttaatttacacttaaaagaaagcccaacgccccaactctcctgctttacgcctgataatgatccgatatgccacaaggatttcggacaaaggggcttactatgctatgacgagtagagtggaataagacttcgacaccacctaaaatttcataaggcacccgcttgatttgtaggcgaatcaggcgggtgcctcttttatttcggctctcgaaaaagcctttttcggcctgcgatttatatacaaatcactttcaagctatatataaatggaaaacgatctgtatatttattgcaaatgatttatatataaatcgtttccagatctcttttagatcaaaactcaaactgaaaaagtccgttttctcatttcttcgacttcaaaatccgcttccattcaggtgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggctcgtatttgggtattgcgaaaacataaaggagaattgcaagccaaagagataaaaaaggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG1145:PG1148|PG1145:PG1148:long-chain-fatty-acid--CoA ligase, putative:hypothetical protein:<-->:1230396..1231343 948 catgaaatcgatattttgtcggcatctgttttattgccgccgtttccgagcacaaaggtagtcttatttggcaactgaccgtcaactaagaaaaaagataatatacttgttgtacagcacaaaccttatgcttatcaagttactttttgtatgattacgtttgtataattacacatagagagatttgtatacgtcaataaaataagctaagctttgaaagctcaatcaatttaaatagtccacattaacaactaatttgaacaaaaaaggtgtcctaaaaatttttgctacctttgcaaggtctgataaggatctcttccatagagccgagagatgcataggccaaagcaagcgtttcttcttgctggacacaagtaaatagaagatatattgtactgataatgattggacgattaaagacttcggcttttgcttttaatttacacttaaaagaaagcccaacgccccaactctcctgctttacgcctgataatgatccgatatgccacaaggatttcggacaaaggggcttactatgctatgacgagtagagtggaataagacttcgacaccacctaaaatttcataaggcacccgcttgatttgtaggcgaatcaggcgggtgcctcttttatttcggctctcgaaaaagcctttttcggcctgcgatttatatacaaatcactttcaagctatatataaatggaaaacgatctgtatatttattgcaaatgatttatatataaatcgtttccagatctcttttagatcaaaactcaaactgaaaaagtccgttttctcatttcttcgacttcaaaatccgcttccattcaggtgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggctcgtatttgggtattgcgaaaacataaaggagaattgcaagccaaagagataaaaaaggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 catgaaatcgatattttgtcggcatctgttttattgccgccgtttccgagcacaaaggtagtcttatttggcaactgaccgtcaactaagaaaaaagataatatacttgttgtacagcacaaaccttatgcttatcaagttactttttgtatgattacgtttgtataattacacatagagagatttgtatacgtcaataaaataagctaagctttgaaagctcaatcaatttaaatagtccacattaacaactaatttgaacaaaaaaggtgtcctaaaaatttttgctacctttgcaaggtctgataaggatctcttccatagagccgagagatgcataggccaaagcaagcgtttcttcttgctggacacaagtaaatagaagatatattgtactgataatgattggacgattaaagacttcggcttttgcttttaatttacacttaaaagaaagcccaacgccccaactctcctgctttacgcctgataatgatccgatatgccacaaggatttcggacaaaggggcttactatgctatgacgagtagagtggaataagacttcgacaccacctaaaatttcataaggcacccgcttgatttgtaggcgaatcaggcgggtgcctcttttatttcggctctcgaaaaagcctttttcggcctgcgatttatatacaaatcactttcaagctatatataaatggaaaacgatctgtatatttattgcaaatgatttatatataaatcgtttccagatctcttttagatcaaaactcaaactgaaaaagtccgttttctcatttcttcgacttcaaaatccgcttccattcaggtgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggctcgtatttgggtattgcgaaaacataaaggagaattgcaagccaaagagataaaaaaggc Predict ORF larger than 30AA ================================================ Protein_Len: 41 Strand: + Start: 257 End: 379 ................................................................................................................................................................................................................................................................ M N K K G V L K I F A T F A R S D K D L F H R A E R C I G Q S K R F F L L D T S K ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 53 Strand: + Start: 390 End: 548 ..................................................................................................................................................................................................................................................................................................................................................................................................... M V L I M I G R L K T S A F A F N L H L K E S P T P Q L S C F T P D N D P I C H K D F G Q R G L L C Y D E ................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 48 Strand: + Start: 769 End: 912 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M K T Q T E K V R F L I S S T S K S A S I Q V L I W N H S K L V F V V E N F F G S Y L G I A K T .................................... Protein_Len: 60 Strand: + Start: 599 End: 946 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M I C R R I R R V P L L F R L S K K P F S A C D L Y T N H F Q A I Y K W K T I C I F I A N D L Y I N R F Q I S F R S K L .. Protein_Len: 41 Strand: - Start: 817 End: 939 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ F G S G N L H K N P I M G F E D E N N F V K K A R I Q T N R F C L P S N C A L S M ......... Protein_Len: 30 Strand: - Start: 691 End: 780 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. A I Y L H F V I Q I N I A F S K Y I F R K W I E K L D F S M ........................................................................................................................................................................ Protein_Len: 45 Strand: - Start: 484 End: 618 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... A Q Y H D S I G C P N R V F P S V I S H R T S H F L V E V G G L I E Y P V R K I Q L R I M .......................................................................................................................................................................................................................................................................................................................................... Protein_Len: 33 Strand: - Start: 291 End: 389 .................................................................................................................................................................................................................................................................................................. R Q L T Q Y P D R G Y L R S I C L G F C A N R R A P C L Y I S S M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 278 End: 511 ..................................................................................................................................................................................................................................................................................... S A Y A L A L T E E Q Q V C T F L L Y I T S I I I P R N F V E A K A K L K C K F S L G V G W S E Q K V G S L S G I H W M ..................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 229 End: 414 .................................................................................................................................................................................................................................... Y D V N V V L K F L F P T R F I K A V K A L D S L S R K W L A S L H M P W L L R K K K S S V L L Y F I Y Q V S L S Q V M ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 40 Strand: - Start: 3 End: 122 .. S I S I K D A D T K N G G N G L V F T T K N P L Q G D V L F F S L I S T T C C M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... catgaaatcgatattttgtcggcatctgttttattgccgccgtttccgagcacaaaggtagtcttatttggcaactgaccgtcaactaagaaaaaagataatatacttgttgtacagcacaaaccttatgcttatcaagttactttttgtatgattacgtttgtataattacacatagagagatttgtatacgtcaataaaataagctaagctttgaaagctcaatcaatttaaatagtccacattaacaactaatttgaacaaaaaaggtgtcctaaaaatttttgctacctttgcaaggtctgataaggatctcttccatagagccgagagatgcataggccaaagcaagcgtttcttcttgctggacacaagtaaatagaagatatattgtactgataatgattggacgattaaagacttcggcttttgcttttaatttacacttaaaagaaagcccaacgccccaactctcctgctttacgcctgataatgatccgatatgccacaaggatttcggacaaaggggcttactatgctatgacgagtagagtggaataagacttcgacaccacctaaaatttcataaggcacccgcttgatttgtaggcgaatcaggcgggtgcctcttttatttcggctctcgaaaaagcctttttcggcctgcgatttatatacaaatcactttcaagctatatataaatggaaaacgatctgtatatttattgcaaatgatttatatataaatcgtttccagatctcttttagatcaaaactcaaactgaaaaagtccgttttctcatttcttcgacttcaaaatccgcttccattcaggtgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggctcgtatttgggtattgcgaaaacataaaggagaattgcaagccaaagagataaaaaaggc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ catgaaatcgatattttgtcggcatctgttttattgccgccgtttccgagcacaaaggtagtcttatttggcaactgaccgtcaactaagaaaaaagataatatacttgttgtacagcacaaaccttatgcttatcaagttactttttgtatgattacgtttgtataattacacatagagagatttgtatacgtcaataaaataagctaagctttgaaagctcaatcaatttaaatagtccacattaacaactaatttgaacaaaaaaggtgtcctaaaaatttttgctacctttgcaaggtctgataaggatctcttccatagagccgagagatgcataggccaaagcaagcgtttcttcttgctggacacaagtaaatagaagatatattgtactgataatgattggacgattaaagacttcggcttttgcttttaatttacacttaaaagaaagcccaacgccccaactctcctgctttacgcctgataatgatccgatatgccacaaggatttcggacaaaggggcttactatgctatgacgagtagagtggaataagacttcgacaccacctaaaatttcataaggcacccgcttgatttgtaggcgaatcaggcgggtgcctcttttatttcggctctcgaaaaagcctttttcggcctgcgatttatatacaaatcactttcaagctatatataaatggaaaacgatctgtatatttattgcaaatgatttatatataaatcgtttccagatctcttttagatcaaaactcaaactgaaaaagtccgttttctcatttcttcgacttcaaaatccgcttccattcaggtgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggctcgtatttgggtattgcgaaaacataaaggagaattgcaagccaaagagataaaaaaggc Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 48 HP_score: -2.7 Tail_Score: -3.64103 Start: 348 End: 365 Full_Region: atttacttgtgtcca gcaag aagaaacg cttgc tttggcctatgcatc ...........................................................................................................................................................................................................................................................................................................................................................gcaagaagaaacgcttgc....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -20.3 Tail_Score: -4.89521 Start: 588 End: 629 Full_Region: CACCTAAAATTTCAT AAGGCACCCGCTTGATTTG TAGG CGAATCAGGCGGGTGCCTC TTTTATTTCGGCTCT ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................AAGGCACCCGCTTGATTTGTAGGCGAATCAGGCGGGTGCCTC............................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -22.9 Tail_Score: -4.06281 Start: 590 End: 627 Full_Region: CCTAAAATTTCATAA GGCACCCGCTTGATTTG TAGG CGAATCAGGCGGGTGCC TCTTTTATTTCGGCT .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GGCACCCGCTTGATTTGTAGGCGAATCAGGCGGGTGCC................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 100 HP_score: -18.5 Tail_Score: -3.42366 Start: 590 End: 627 Full_Region: agccgaaataaaaga ggcacccgcctgattcg ccta caaatcaagcgggtgcc ttatgaaattttagg .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggcacccgcctgattcgcctacaaatcaagcgggtgcc................................................................................................................................................................................................................................................................................................................................. Find igs database================================================ Query_seq: PG1145:PG1148|PG1145:PG1148:long-chain-fatty-acid--CoA ligase, putative:hypothetical protein:<-->:1230396..1231343 948 catgaaatcgatattttgtcggcatctgttttattgccgccgtttccgagcacaaaggtagtcttatttggcaactgaccgtcaactaagaaaaaagataatatacttgttgtacagcacaaaccttatgcttatcaagttactttttgtatgattacgtttgtataattacacatagagagatttgtatacgtcaataaaataagctaagctttgaaagctcaatcaatttaaatagtccacattaacaactaatttgaacaaaaaaggtgtcctaaaaatttttgctacctttgcaaggtctgataaggatctcttccatagagccgagagatgcataggccaaagcaagcgtttcttcttgctggacacaagtaaatagaagatatattgtactgataatgattggacgattaaagacttcggcttttgcttttaatttacacttaaaagaaagcccaacgccccaactctcctgctttacgcctgataatgatccgatatgccacaaggatttcggacaaaggggcttactatgctatgacgagtagagtggaataagacttcgacaccacctaaaatttcataaggcacccgcttgatttgtaggcgaatcaggcgggtgcctcttttatttcggctctcgaaaaagcctttttcggcctgcgatttatatacaaatcactttcaagctatatataaatggaaaacgatctgtatatttattgcaaatgatttatatataaatcgtttccagatctcttttagatcaaaactcaaactgaaaaagtccgttttctcatttcttcgacttcaaaatccgcttccattcaggtgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggctcgtatttgggtattgcgaaaacataaaggagaattgcaagccaaagagataaaaaaggc Intra-Species Hit: Count: 8 Min: 1 Max: 948 Len: 948 Subject: NC_002950_PG1145_PG1148|long-chain-fatty-acid--CoA ligase, putative:hypothetical protein|NEGATIVE:POSITIVE|[1230396,1231343]|948 HSP 1 e-value: 0.0 bit: 1879.0 Len: 948 Query Start:1 Query End:948 Subject Strand: POSITIVE Subject Start: 1 Subject End: 948 catgaaatcgatattttgtcggcatctgttttattgccgccgtttccgagcacaaaggtagtcttatttggcaactgaccgtcaactaagaaaaaagataatatacttgttgtacagcacaaaccttatgcttatcaagttactttttgtatgattacgtttgtataattacacatagagagatttgtatacgtcaataaaataagctaagctttgaaagctcaatcaatttaaatagtccacattaacaactaatttgaacaaaaaaggtgtcctaaaaatttttgctacctttgcaaggtctgataaggatctcttccatagagccgagagatgcataggccaaagcaagcgtttcttcttgctggacacaagtaaatagaagatatattgtactgataatgattggacgattaaagacttcggcttttgcttttaatttacacttaaaagaaagcccaacgccccaactctcctgctttacgcctgataatgatccgatatgccacaaggatttcggacaaaggggcttactatgctatgacgagtagagtggaataagacttcgacaccacctaaaatttcataaggcacccgcttgatttgtaggcgaatcaggcgggtgcctcttttatttcggctctcgaaaaagcctttttcggcctgcgatttatatacaaatcactttcaagctatatataaatggaaaacgatctgtatatttattgcaaatgatttatatataaatcgtttccagatctcttttagatcaaaactcaaactgaaaaagtccgttttctcatttcttcgacttcaaaatccgcttccattcaggtgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggctcgtatttgggtattgcgaaaacataaaggagaattgcaagccaaagagataaaaaaggc catgaaatcgatattttgtcggcatctgttttattgccgccgtttccgagcacaaaggtagtcttatttggcaactgaccgtcaactaagaaaaaagataatatacttgttgtacagcacaaaccttatgcttatcaagttactttttgtatgattacgtttgtataattacacatagagagatttgtatacgtcaataaaataagctaagctttgaaagctcaatcaatttaaatagtccacattaacaactaatttgaacaaaaaaggtgtcctaaaaatttttgctacctttgcaaggtctgataaggatctcttccatagagccgagagatgcataggccaaagcaagcgtttcttcttgctggacacaagtaaatagaagatatattgtactgataatgattggacgattaaagacttcggcttttgcttttaatttacacttaaaagaaagcccaacgccccaactctcctgctttacgcctgataatgatccgatatgccacaaggatttcggacaaaggggcttactatgctatgacgagtagagtggaataagacttcgacaccacctaaaatttcataaggcacccgcttgatttgtaggcgaatcaggcgggtgcctcttttatttcggctctcgaaaaagcctttttcggcctgcgatttatatacaaatcactttcaagctatatataaatggaaaacgatctgtatatttattgcaaatgatttatatataaatcgtttccagatctcttttagatcaaaactcaaactgaaaaagtccgttttctcatttcttcgacttcaaaatccgcttccattcaggtgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggctcgtatttgggtattgcgaaaacataaaggagaattgcaagccaaagagataaaaaaggc Subject: NC_002950_PG1356_PG1357|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1435940,1436416]|477 HSP 1 e-value: 7.0E-25 bit: 117.0 Len: 95 Query Start:620 Query End:714 Subject Strand: POSITIVE Subject Start: 313 Subject End: 407 ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cgggtgcctcttttatttcggctctcgaaaaagcctttttcggcctgcgatttatatacaaatcactttcaagctatatataaatggaaaacgat.......................................................................................................................................................................................................................................... ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cgggtgcctcttttatttcagctccagaaaaggcctttttcagcctgcgatttatatagaaatcattttcaatctatatataaatgaaaaacgat.......................................................................................................................................................................................................................................... HSP 2 e-value: 9.0E-6 bit: 54.0 Len: 59 Query Start:694 Query End:752 Subject Strand: POSITIVE Subject Start: 409 Subject End: 467 .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tatatataaatggaaaacgatctgtatatttattgcaaatgatttatatataaatcgtt.................................................................................................................................................................................................... .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tatatacaaattgaaaacgatctatatatttattgaaatcgttttatatacaaatcgtt.................................................................................................................................................................................................... Subject: NC_002950_PG0404_PG0408|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[440197,441041]|845 HSP 1 e-value: 1.0E-20 bit: 103.0 Len: 52 Query Start:836 Query End:887 Subject Strand: POSITIVE Subject Start: 626 Subject End: 677 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttgg............................................................. ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttgg............................................................. HSP 2 e-value: 6.0E-10 bit: 67.9 Len: 97 Query Start:618 Query End:714 Subject Strand: POSITIVE Subject Start: 386 Subject End: 482 .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggcgggtgcctcttttatttcggctctcgaaaaagcctttttcg-gcctgcgatttatatacaaatcactttcaagctatatataaatggaaaacgat.......................................................................................................................................................................................................................................... .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggcgggtgcttcttttatatcggct-tcggaaaaggccttttcgagcctgcgatttatatacgaatcacttacgatctatatatttattgaaaacgat.......................................................................................................................................................................................................................................... Subject: NC_002950_PG1375_PG1378|hypothetical protein:A/G-specific adenine glycosylase|POSITIVE:NEGATIVE|[1456804,1457804]|1001 HSP 1 e-value: 3.0E-18 bit: 95.6 Len: 104 Query Start:782 Query End:885 Subject Strand: NEGATIVE Subject Start: 321 Subject End: 423 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ctgaaaaagtccgttttctcatttcttcgacttcaaaatccgcttccattcaggtgcttatttggaatcattccaaactcgtcttcgttgttgaaaactttttt............................................................... .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ctgaaaaagttcgttt-ctcacttttttgtcttcaaaatccgtttccatttacgtgcttatttggaatgattctaaactcatcttcattgttgaaaactttttt............................................................... Subject: NC_002950_PG1411_PG1414|potassium uptake protein TrkA, putative:hypothetical protein|NEGATIVE:POSITIVE|[1495257,1497148]|1892 HSP 1 e-value: 1.0E-17 bit: 93.7 Len: 99 Query Start:782 Query End:880 Subject Strand: NEGATIVE Subject Start: 1088 Subject End: 1186 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ctgaaaaagtccgttttctcatttcttcgacttcaaaatccgcttccattcaggtgcttatttggaatcattccaaactcgtcttcgttgttgaaaact.................................................................... .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ctgaaaaagttcgtttcttcatattttcgacatcaaaatccgttccctttcaggtgcttatttagaatcattccaaattccgcttcgttgttgaaaact.................................................................... HSP 2 e-value: 7.0E-16 bit: 87.7 Len: 92 Query Start:619 Query End:710 Subject Strand: NEGATIVE Subject Start: 1280 Subject End: 1371 ..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gcgggtgcctcttttatttcggctctcgaaaaagcctttttcggcctgcgatttatatacaaatcactttcaagctatatataaatggaaaa.............................................................................................................................................................................................................................................. ..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gcgggtgcctcttttatttcagcttccgaaagggcctttttcggtcttcgatttatatacaaatgagtttcgatctatatataaattgaaaa.............................................................................................................................................................................................................................................. Subject: NC_002950_PG0965_PG0968|phosphatidylserine decarboxylase:Mrr restriction system protein|NEGATIVE:POSITIVE|[1025867,1026967]|1101 HSP 1 e-value: 4.0E-14 bit: 81.8 Len: 53 Query Start:833 Query End:885 Subject Strand: POSITIVE Subject Start: 770 Subject End: 822 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................aggtgcttatttggaatcattccaaactcgtcttcgttgttgaaaactttttt............................................................... ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................aggttcttatttggaatgattccaaactcatcttcgttgttgaaaactttttt............................................................... HSP 2 e-value: 2.0E-7 bit: 60.0 Len: 62 Query Start:653 Query End:714 Subject Strand: POSITIVE Subject Start: 568 Subject End: 629 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cctttttcggcctgcgatttatatacaaatcactttcaagctatatataaatggaaaacgat.......................................................................................................................................................................................................................................... ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cctttttcagcctgcgatttatatagaaatgagtttcgatctatgtataaattgaaaacgat.......................................................................................................................................................................................................................................... Subject: NC_002950_PG1119_PG1121|flavodoxin, putative:asparaginyl-tRNA synthetase|POSITIVE:NEGATIVE|[1195544,1196235]|692 HSP 1 e-value: 1.0E-11 bit: 73.8 Len: 69 Query Start:830 Query End:898 Subject Strand: NEGATIVE Subject Start: 147 Subject End: 215 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttcaggtgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggctcgtatttgg.................................................. .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttcaggcgtttatttagaatgattccaaacttgtcttcgttgttgaaaagttttttggtgcgtatttgg.................................................. HSP 2 e-value: 6.0E-7 bit: 58.0 Len: 57 Query Start:696 Query End:752 Subject Strand: NEGATIVE Subject Start: 293 Subject End: 349 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tatataaatggaaaacgatctgtatatttattgcaaatgatttatatataaatcgtt.................................................................................................................................................................................................... .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tatataaatcgaaaacgatctgtatataaattaaaaatgctttatatatagatcgtt.................................................................................................................................................................................................... Subject: NC_002950_PG0022_PG0024|sulfate permease family protein:redox-sensing transcriptional repressor Rex|NEGATIVE:POSITIVE|[26887,27871]|985 HSP 1 e-value: 2.0E-10 bit: 69.9 Len: 47 Query Start:837 Query End:883 Subject Strand: NEGATIVE Subject Start: 237 Subject End: 283 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gcttatttggaatcattccaaactcgtcttcgttgttgaaaactttt................................................................. ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gcttatttagaatgattccaaactcttcttcgttgttgaaaactttt................................................................. HSP 2 e-value: 6.0E-7 bit: 58.0 Len: 57 Query Start:694 Query End:750 Subject Strand: NEGATIVE Subject Start: 370 Subject End: 426 .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tatatataaatggaaaacgatctgtatatttattgcaaatgatttatatataaatcg...................................................................................................................................................................................................... .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tatatataaatcgaaaacgatctgtatataaattgaaactgattaatatatacatcg...................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAUGAAAUCGAUAUUUUGUCGGCAUCUGUUUUAUUGCCGCCGUUUCCGAGCACAAAGGUAGUCUUAUUUGGCAACUGACCGUCAACUAAGAAAAAAGAUAAUAUACUUGUUGUACAGCACAAACCUUAUGCUUAUCAAGUUACUUUUUGUAUGAUUACGUUUGUAUAAUUACACAUAGAGAGAUUUGUAUACGUCAAUAAAAUAAGCUAAGCUUUGAAAGCUCAAUCAAUUUAAAUAGUCCACAUUAACAACUAAUUUGAACAAAAAAGGUGUCCUAAAAAUUUUUGCUACCUUUGCAAGGUCUGAUAAGGAUCUCUUCCAUAGAGCCGAGAGAUGCAUAGGCCAAAGCAAGCGUUUCUUCUUGCUGGACACAAGUAAAUAGAAGAUAUAUUGUACUGAUAAUGAUUGGACGAUUAAAGACUUCGGCUUUUGCUUUUAAUUUACACUUAAAAGAAAGCCCAACGCCCCAACUCUCCUGCUUUACGCCUGAUAAUGAUCCGAUAUGCCACAAGGAUUUCGGACAAAGGGGCUUACUAUGCUAUGACGAGUAGAGUGGAAUAAGACUUCGACACCACCUAAAAUUUCAUAAGGCACCCGCUUGAUUUGUAGGCGAAUCAGGCGGGUGCCUCUUUUAUUUCGGCUCUCGAAAAAGCCUUUUUCGGCCUGCGAUUUAUAUACAAAUCACUUUCAAGCUAUAUAUAAAUGGAAAACGAUCUGUAUAUUUAUUGCAAAUGAUUUAUAUAUAAAUCGUUUCCAGAUCUCUUUUAGAUCAAAACUCAAACUGAAAAAGUCCGUUUUCUCAUUUCUUCGACUUCAAAAUCCGCUUCCAUUCAGGUGCUUAUUUGGAAUCAUUCCAAACUCGUCUUCGUUGUUGAAAACUUUUUUGGCUCGUAUUUGGGUAUUGCGAAAACAUAAAGGAGAAUUGCAAGCCAAAGAGAUAAAAAAGGC .(((((((........((((((..(((((((((((.(..(((((....((((....((((.(((((.(((((........))))).)))))............(((((((.((.(((((((((.((((.((((((((((((.((((.(((.((((((........)))))).))).)))).))))))..............)))))))))).))))...((((......((((((((((...........))).))))))).....(((..(((((......((((((.......)))))).......)))))..))).....)))).(((((((((....((....))..)))))))))..))))).)))))))))....((((....(((((.(..((....))..))))))......))))(((((...(((((((.......))))))).)))))....(((((.....((..((.....))..))...((.(((((....((....))...)))))))..))))).)))).))))..)))).)..))))))))).)))..)))))).........))))))).((((((((((((((((((....)))))))))))))))))).(((((((((((((...(((((...(((((((((...((((((((((((.((((((.((.(((...(((((((.((.(....).)).)))))))...))).)).)))))).)))))))))))).....(((((.....)))))..........)))))))))....))))).((.((((((............(((....((((((((((...((((((.....))))))............((..(((....)))..))..))))))))))...)))..........)))))).))..))))...)))))))))..... (-249.03) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCCUUUUUUAUCUCUUUGGCUUGCAAUUCUCCUUUAUGUUUUCGCAAUACCCAAAUACGAGCCAAAAAAGUUUUCAACAACGAAGACGAGUUUGGAAUGAUUCCAAAUAAGCACCUGAAUGGAAGCGGAUUUUGAAGUCGAAGAAAUGAGAAAACGGACUUUUUCAGUUUGAGUUUUGAUCUAAAAGAGAUCUGGAAACGAUUUAUAUAUAAAUCAUUUGCAAUAAAUAUACAGAUCGUUUUCCAUUUAUAUAUAGCUUGAAAGUGAUUUGUAUAUAAAUCGCAGGCCGAAAAAGGCUUUUUCGAGAGCCGAAAUAAAAGAGGCACCCGCCUGAUUCGCCUACAAAUCAAGCGGGUGCCUUAUGAAAUUUUAGGUGGUGUCGAAGUCUUAUUCCACUCUACUCGUCAUAGCAUAGUAAGCCCCUUUGUCCGAAAUCCUUGUGGCAUAUCGGAUCAUUAUCAGGCGUAAAGCAGGAGAGUUGGGGCGUUGGGCUUUCUUUUAAGUGUAAAUUAAAAGCAAAAGCCGAAGUCUUUAAUCGUCCAAUCAUUAUCAGUACAAUAUAUCUUCUAUUUACUUGUGUCCAGCAAGAAGAAACGCUUGCUUUGGCCUAUGCAUCUCUCGGCUCUAUGGAAGAGAUCCUUAUCAGACCUUGCAAAGGUAGCAAAAAUUUUUAGGACACCUUUUUUGUUCAAAUUAGUUGUUAAUGUGGACUAUUUAAAUUGAUUGAGCUUUCAAAGCUUAGCUUAUUUUAUUGACGUAUACAAAUCUCUCUAUGUGUAAUUAUACAAACGUAAUCAUACAAAAAGUAACUUGAUAAGCAUAAGGUUUGUGCUGUACAACAAGUAUAUUAUCUUUUUUCUUAGUUGACGGUCAGUUGCCAAAUAAGACUACCUUUGUGCUCGGAAACGGCGGCAAUAAAACAGAUGCCGACAAAAUAUCGAUUUCAUG ...((((((((.((((((((((.(((.(((.((((...((...((........................((((((......))))))..(((((((.....)))))))..))....))...)))).)))..))))))))))))).))))))))..((((((((((.(((((..((..(((((.....)))))..))..(((((((((((((((((((((.(((...(((((.((((.(....).)))).)))))...))).)))))))))))))))))))))))))).))))))).((((((((((((((((.......(((((((((((.(((((........))))).)))))))))))...........(((((.((.(((......))).)).))))).......((((((...(((.....((((((.((((....)).)).))))))......(((((((......(((((...(((((.((.(((((((((((((.......)))))))..)))))).)))))))...)).))).....................((((((.......(((.....))))))))).)))))))....))))))))).....)))))))).)))))))).)))..(((.......(((((((((((...........(((((.......)))))....((..((....(((.(((((...(((..(((..(((...((((.((((((((((((((((...(....)...........(((((.(((((......))))).)))))...)))))...))))))).)))).)))).)))((((.....))))...)))..)))...))))).)))..))..))........).))))))))))...((....)).(((((..........)))))..........)))...... (-256.21) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 960 80 31 171 122 NC_002950:PG1145|5end_long-chain-fatty-acid--CoA_ligase,_putative_1230305..1230535_NEGATIVE 705 890 841 69 20 NC_002950:PG0773|5end_hypothetical_protein_825131..825361_POSITIVE 703 888 841 122 75 NC_002950:PG0609|5end_hypothetical_protein_663183..663413_POSITIVE 685 890 841 87 38 NC_002950:PG1357|5end_hypothetical_protein_1436458..1436688_NEGATIVE 648 670 621 121 71 NC_002950:PG1223|5end_hypothetical_protein_1299461..1299691_POSITIVE 639 880 834 47 1 NC_002950:PG0995|5end_hypothetical_protein_1055585..1055815_NEGATIVE 479 670 621 75 26 NC_002950:PG1574|5end_hypothetical_protein_1652613..1652843_NEGATIVE 479 670 621 81 32 NC_002950:PG0371|5end_hypothetical_protein_404043..404273_POSITIVE 450 625 585 135 93 NC_002950:PG1695|5end_hypothetical_protein_1779440..1779670_POSITIVE 440 519 471 68 12 NC_002950:PG0661|5end_hypothetical_protein_709853..710083_NEGATIVE 440 519 471 226 170 NC_002950:PG0660|5end_nitroreductase_family_protein_709695..709925_NEGATIVE 405 637 595 87 36 NC_002950:PG0065|5end_efflux_transporter,_RND_family,_MFP_subunit_81187..81417_NEGATIVE 403 549 501 77 26 NC_002950:PG0214|5end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_250963..251193_POSITIVE 395 364 323 231 178 NC_002950:PG0762|5end_trigger_factor,_putative_812759..812989_POSITIVE 389 369 321 60 5 NC_002950:PG1878|5end_cysteinyl-tRNA_synthetase_1972928..1973158_POSITIVE 379 837 793 97 55 NC_002950:PG1619|5end_biotin_synthesis_protein_BioC,_putative_1698568..1698798_POSITIVE 374 356 314 67 23 NC_002950:PG0083|5end_hypothetical_protein_96952..97182_POSITIVE 369 369 321 57 2 NC_002950:PG1022|5end_hypothetical_protein_1083537..1083767_POSITIVE 362 648 605 194 142 NC_002950:PG1482|5end_conjugative_transposon_protein_TraF_1558779..1559009_NEGATIVE 362 838 792 152 94 NC_002950:PG0203|5end_hypothetical_protein_240211..240441_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 729 880 833 48 1 NC_002950:PG1868|3end_hypothetical_protein_1965098..1965248_NEGATIVE 705 890 841 62 13 NC_002950:PG0772|3end_hypothetical_protein_825204..825354_POSITIVE 571 870 821 147 98 NC_002950:PG1167|3end_hypothetical_protein_1248644..1248794_POSITIVE 529 868 821 151 96 NC_002950:PG0995|3end_hypothetical_protein_1055469..1055619_NEGATIVE 450 630 585 53 10 NC_002950:PG0568|3end_elongation_factor_P_623999..624149_NEGATIVE 425 760 711 88 39 NC_002950:PG1357|3end_hypothetical_protein_1436327..1436477_NEGATIVE 418 628 585 53 10 NC_002950:PG1274|3end_elongation_factor_P_1352489..1352639_POSITIVE 418 48 7 136 97 NC_002950:PG0443|3end_hemagglutinin-related_protein_483147..483297_POSITIVE 416 635 591 147 102 NC_002950:PG0371|3end_hypothetical_protein_404224..404374_POSITIVE 400 906 863 50 1 NC_002950:PG0095|3end_DNA_mismatch_repair_protein_112088..112238_NEGATIVE 383 350 310 110 73 NC_002950:PG1391|3end_hypothetical_protein_1472961..1473111_POSITIVE 379 837 793 118 76 NC_002950:PG1618|3end_hypothetical_protein_1698669..1698819_POSITIVE 374 356 314 61 17 NC_002950:PG0082|3end_hypothetical_protein_97026..97176_POSITIVE 362 838 792 101 43 NC_002950:PG0204|3end_hypothetical_protein_240262..240412_NEGATIVE 355 740 694 56 10 NC_002950:PG0773|3end_hypothetical_protein_825348..825498_POSITIVE 354 65 21 44 1 NC_002950:PG2161|3end_transcriptional_regulator,_AraC_family_2266717..2266867_NEGATIVE 353 628 589 55 16 NC_002950:PG1866|3end_hypothetical_protein_1964736..1964886_POSITIVE 353 639 591 71 26 NC_002950:PG1482|3end_conjugative_transposon_protein_TraF_1558444..1558594_NEGATIVE 347 54 15 148 109 NC_002950:PG1589|3end_dihydropteroate_synthase_1666593..1666743_NEGATIVE 344 50 17 146 109 NC_002950:PG0003|3end_hypothetical_protein_1919..2069_NEGATIVE