Origin IGS: tatataatggtattcattatttgtgtttatattattcgaaagctcccattctaaggttatttacctcctgttcattcagatagcgccattttccacggggcagattcttcttggtcagtccggcaaaataaacgcgatccagtttgacaacacgatagccaaggtgttcaaacaaacggcgcacaatacgatttcgtccgctatgaatctctattcccacctgcgaaagatcgtcttcttttacgtaactgatggcatcggcatgtatctcaccatcatccaattccacgccgtcggctattttctgcatgtcttcaatgcttacatctttattgagccacacgtggtagatcttcttcttcttgaaagacggatgggtcagcttagaggcaagatcgccatcatttgtaatcagaagtacaccggtagtattccgatccaaacgtcctaccggatatattctctcaggacaggcatatcgtactatatccattactgtggtacggcattcggggtcatcagaagtggtcacacaatttttaggcttattgagcagtacataaactttactctctatttccactctcttatccttgtagattacctcatcctgacgtgtaattcttctaccgagttcggtcacgacttctccattgaccgtgatctctcccttttcgatcagcagatccgcttcccttcgcgagcaaacaccggcattgctcaagaacttgttcagtctgatcggctcattcggatcggccagtacctcacgatactttactggcacaatcggagctttcgactgtttctttttcttcttttgctgtggaagtgtcagtgttctattgccacccgaatacatcggcctttccgtatcccgaaactgacggggataagatggtcccgaaccacgtatacgctcgctgtccatagcatgattggcatcgtactctttctcaaaatgtctgagagaatcgcgtctctgcccacgctcttcattttggcggtatgaagccggataagcagcgcgctgcccttcccgactatatcgattgttgtacgaaggagcttcagagcggttatttccttcccctccggagaaatcatcacgtctgtacgattgtgatcgactgctctgctgctcaccgatacgtactcgtcgtttacgctgttgcaattgatccgtcggttcgggtttggatgtagcccctactctgggagtaggtctgaaacctccgcctgaactcattcttgacgaacggaaaggcgtaggtttttctatgtgcttttccgaaggagaaatatcatccttgctcgtatctgcgaaaatatcgcgttcttcagttgtcatctttgatagattattgcttagattttacacatacaacctcacggttgcaaactatggatttgtcttttttgactctttgggttacgaaagtatgaaaatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aattttcatactttcgtaacccaaagagtcaaaaaagacaaatccatagtttgcaaccgtgaggttgtatgtgtaaaatctaagcaataatctatcaaagatgacaactgaagaacgcgatattttcgcagatacgagcaaggatgatatttctccttcggaaaagcacatagaaaaacctacgcctttccgttcgtcaagaatgagttcaggcggaggtttcagacctactcccagagtaggggctacatccaaacccgaaccgacggatcaattgcaacagcgtaaacgacgagtacgtatcggtgagcagcagagcagtcgatcacaatcgtacagacgtgatgatttctccggaggggaaggaaataaccgctctgaagctccttcgtacaacaatcgatatagtcgggaagggcagcgcgctgcttatccggcttcataccgccaaaatgaagagcgtgggcagagacgcgattctctcagacattttgagaaagagtacgatgccaatcatgctatggacagcgagcgtatacgtggttcgggaccatcttatccccgtcagtttcgggatacggaaaggccgatgtattcgggtggcaatagaacactgacacttccacagcaaaagaagaaaaagaaacagtcgaaagctccgattgtgccagtaaagtatcgtgaggtactggccgatccgaatgagccgatcagactgaacaagttcttgagcaatgccggtgtttgctcgcgaagggaagcggatctgctgatcgaaaagggagagatcacggtcaatggagaagtcgtgaccgaactcggtagaagaattacacgtcaggatgaggtaatctacaaggataagagagtggaaatagagagtaaagtttatgtactgctcaataagcctaaaaattgtgtgaccacttctgatgaccccgaatgccgtaccacagtaatggatatagtacgatatgcctgtcctgagagaatatatccggtaggacgtttggatcggaatactaccggtgtacttctgattacaaatgatggcgatcttgcctctaagctgacccatccgtctttcaagaagaagaagatctaccacgtgtggctcaataaagatgtaagcattgaagacatgcagaaaatagccgacggcgtggaattggatgatggtgagatacatgccgatgccatcagttacgtaaaagaagacgatctttcgcaggtgggaatagagattcatagcggacgaaatcgtattgtgcgccgtttgtttgaacaccttggctatcgtgttgtcaaactggatcgcgtttattttgccggactgaccaagaagaatctgccccgtggaaaatggcgctatctgaatgaacaggaggtaaataaccttagaatgggagctttcgaataatataaacacaaataatgaataccattatata Mask Tandem Repeat Region ================================================ tatataatggtattcattatttgtgtttatattattcgaaagctcccattctaaggttatttacctcctgttcattcagatagcgccattttccacggggcagattcttcttggtcagtccggcaaaataaacgcgatccagtttgacaacacgatagccaaggtgttcaaacaaacggcgcacaatacgatttcgtccgctatgaatctctattcccacctgcgaaagatcgtcttcttttacgtaactgatggcatcggcatgtatctcaccatcatccaattccacgccgtcggctattttctgcatgtcttcaatgcttacatctttattgagccacacgtggtagatcttcttcttcttgaaagacggatgggtcagcttagaggcaagatcgccatcatttgtaatcagaagtacaccggtagtattccgatccaaacgtcctaccggatatattctctcaggacaggcatatcgtactatatccattactgtggtacggcattcggggtcatcagaagtggtcacacaatttttaggcttattgagcagtacataaactttactctctatttccactctcttatccttgtagattacctcatcctgacgtgtaattcttctaccgagttcggtcacgacttctccattgaccgtgatctctcccttttcgatcagcagatccgcttcccttcgcgagcaaacaccggcattgctcaagaacttgttcagtctgatcggctcattcggatcggccagtacctcacgatactttactggcacaatcggagctttcgactgtttctttttcttcttttgctgtggaagtgtcagtgttctattgccacccgaatacatcggcctttccgtatcccgaaactgacggggataagatggtcccgaaccacgtatacgctcgctgtccatagcatgattggcatcgtactctttctcaaaatgtctgagagaatcgcgtctctgcccacgctcttcattttggcggtatgaagccggataagcagcgcgctgcccttcccgactatatcgattgttgtacgaaggagcttcagagcggttatttccttcccctccggagaaatcatcacgtctgtacgattgtgatcgactgctctgctgctcaccgatacgtactcgtcgtttacgctgttgcaattgatccgtcggttcgggtttggatgtagcccctactctgggagtaggtctgaaacctccgcctgaactcattcttgacgaacggaaaggcgtaggtttttctatgtgcttttccgaaggagaaatatcatccttgctcgtatctgcgaaaatatcgcgttcttcagttgtcatctttgatagattattgcttagattttacacatacaacctcacggttgcaaactatggatttgtcttttttgactctttgggttacgaaagtatgaaaatt Find is-nt database================================================ Query_seq: PG1121:PG1123|PG1121:PG1123:asparaginyl-tRNA synthetase:adenylosuccinate lyase:<-<-:1197646..1199096 1451 tatataatggtattcattatttgtgtttatattattcgaaagctcccattctaaggttatttacctcctgttcattcagatagcgccattttccacggggcagattcttcttggtcagtccggcaaaataaacgcgatccagtttgacaacacgatagccaaggtgttcaaacaaacggcgcacaatacgatttcgtccgctatgaatctctattcccacctgcgaaagatcgtcttcttttacgtaactgatggcatcggcatgtatctcaccatcatccaattccacgccgtcggctattttctgcatgtcttcaatgcttacatctttattgagccacacgtggtagatcttcttcttcttgaaagacggatgggtcagcttagaggcaagatcgccatcatttgtaatcagaagtacaccggtagtattccgatccaaacgtcctaccggatatattctctcaggacaggcatatcgtactatatccattactgtggtacggcattcggggtcatcagaagtggtcacacaatttttaggcttattgagcagtacataaactttactctctatttccactctcttatccttgtagattacctcatcctgacgtgtaattcttctaccgagttcggtcacgacttctccattgaccgtgatctctcccttttcgatcagcagatccgcttcccttcgcgagcaaacaccggcattgctcaagaacttgttcagtctgatcggctcattcggatcggccagtacctcacgatactttactggcacaatcggagctttcgactgtttctttttcttcttttgctgtggaagtgtcagtgttctattgccacccgaatacatcggcctttccgtatcccgaaactgacggggataagatggtcccgaaccacgtatacgctcgctgtccatagcatgattggcatcgtactctttctcaaaatgtctgagagaatcgcgtctctgcccacgctcttcattttggcggtatgaagccggataagcagcgcgctgcccttcccgactatatcgattgttgtacgaaggagcttcagagcggttatttccttcccctccggagaaatcatcacgtctgtacgattgtgatcgactgctctgctgctcaccgatacgtactcgtcgtttacgctgttgcaattgatccgtcggttcgggtttggatgtagcccctactctgggagtaggtctgaaacctccgcctgaactcattcttgacgaacggaaaggcgtaggtttttctatgtgcttttccgaaggagaaatatcatccttgctcgtatctgcgaaaatatcgcgttcttcagttgtcatctttgatagattattgcttagattttacacatacaacctcacggttgcaaactatggatttgtcttttttgactctttgggttacgaaagtatgaaaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG1121:PG1123|PG1121:PG1123:asparaginyl-tRNA synthetase:adenylosuccinate lyase:<-<-:1197646..1199096 1451 tatataatggtattcattatttgtgtttatattattcgaaagctcccattctaaggttatttacctcctgttcattcagatagcgccattttccacggggcagattcttcttggtcagtccggcaaaataaacgcgatccagtttgacaacacgatagccaaggtgttcaaacaaacggcgcacaatacgatttcgtccgctatgaatctctattcccacctgcgaaagatcgtcttcttttacgtaactgatggcatcggcatgtatctcaccatcatccaattccacgccgtcggctattttctgcatgtcttcaatgcttacatctttattgagccacacgtggtagatcttcttcttcttgaaagacggatgggtcagcttagaggcaagatcgccatcatttgtaatcagaagtacaccggtagtattccgatccaaacgtcctaccggatatattctctcaggacaggcatatcgtactatatccattactgtggtacggcattcggggtcatcagaagtggtcacacaatttttaggcttattgagcagtacataaactttactctctatttccactctcttatccttgtagattacctcatcctgacgtgtaattcttctaccgagttcggtcacgacttctccattgaccgtgatctctcccttttcgatcagcagatccgcttcccttcgcgagcaaacaccggcattgctcaagaacttgttcagtctgatcggctcattcggatcggccagtacctcacgatactttactggcacaatcggagctttcgactgtttctttttcttcttttgctgtggaagtgtcagtgttctattgccacccgaatacatcggcctttccgtatcccgaaactgacggggataagatggtcccgaaccacgtatacgctcgctgtccatagcatgattggcatcgtactctttctcaaaatgtctgagagaatcgcgtctctgcccacgctcttcattttggcggtatgaagccggataagcagcgcgctgcccttcccgactatatcgattgttgtacgaaggagcttcagagcggttatttccttcccctccggagaaatcatcacgtctgtacgattgtgatcgactgctctgctgctcaccgatacgtactcgtcgtttacgctgttgcaattgatccgtcggttcgggtttggatgtagcccctactctgggagtaggtctgaaacctccgcctgaactcattcttgacgaacggaaaggcgtaggtttttctatgtgcttttccgaaggagaaatatcatccttgctcgtatctgcgaaaatatcgcgttcttcagttgtcatctttgatagattattgcttagattttacacatacaacctcacggttgcaaactatggatttgtcttttttgactctttgggttacgaaagtatgaaaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 4 Min: 50 Max: 754 Len: 705 Subject: gi|89110743|ref|AP_004523.1| 23S rRNA pseudouridine synthase [Escherichia coli W3110] HSP 1 e-value: 2.0E-32 bit: 137.0 Len: 702 Query Start:53 Query End:754 Subject Strand: null Subject Start: 3 Subject End: 232 .................................................... P N E P I R L N K F L S N A G V C S R R E A D L L I E K G E I T V N G E V V T E L G R R I T R Q D E V I Y K D K R V - - - E I E S K V Y V L L N K P K N C V T T S D D P E C R T T V M D I V R Y A C P E R I Y P V G R L D R N T T G V L L I T N D G D L A S K L T H P S F K K K K I Y H V W L N K D V S I E D M Q K I A D G V E L D D G E I H A D A I S Y V K E D D L S Q V G I E I H S G R N R I V R R L F E H L G Y R V V K L D R V Y F A G L T K K N L P R G K W R Y L N E Q E V N N L ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... .................................................... P D S S V R L N K Y I S E S G I C S R R E A D R Y I E Q G N V F L N G K R A T - I G D Q V K P G D V V K V N G Q L I E P R E A E D L V L I A L N K P V G I V S T T E D G E - R D N I V D F V N H S - - K R V F P I G R L D K D S Q G L I F L T N H G D L V N K I L R A G N D H E K E Y L V T V D K P I T E E F I R G M S A G V P I - L G T V T K K C - - K V K K E A P F V F R I T L V Q G L N R Q I R R M C E H F G Y E V K K L E R T R I M N V S L S G I P L G E W R D L T D D E L I D L ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: gi|89108115|ref|AP_001895.1| 23S rRNA pseudouridylate synthase [Escherichia coli W3110] HSP 1 e-value: 3.0E-27 bit: 120.0 Len: 690 Query Start:50 Query End:739 Subject Strand: null Subject Start: 4 Subject End: 241 ................................................. R L N K F L S N A G V C S R R E A D L L I E K G E I T V N G E V V T E L G R R I T - - - - - - - R Q D E V I Y K D K R V E I E S K V Y V L L N K P K N C V T T S D D P E C R T T V M D I V R Y A C P E R I Y P V G R L D R N T T G V L L I T N D G D L A S K L T H P S F K K K K I Y H V W L N K D V S I E D M Q K I A D G V E L D D G E I H A D A I S Y V K E D D L S Q - V G I E I H S G R N R I V R R L F E H L G Y R V V K L D R V Y F A G L - T K K N L P R G K W R Y L N E Q E V N N L R ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ ................................................. K L Q K V L A R A G H G S R R E I E S I I E A G R V S V D G K I A - K L G D R V E V T P G L K I R I D G H L I S V R E S A E Q I C R V L A Y Y K P E G E L C T R N D P E G R P T V F D R L P K L R G A R W I A V G R L D V N T C G L L L F T T D G E L A N R L M H P S R E V E R E Y A V R V F G Q V D D A K L R D L S R G V Q L E D G P A A F K T I K F S G G E G I N Q W Y N V T L T E G R N R E V R R L W E A V G V Q V S R L I R V R Y G D I P L P K G L P R G G W T E L D L A Q T N Y L R ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Subject: gi|89109001|ref|AP_002781.1| 16S rRNA pseudouridylate 516 synthase [Escherichia coli W3110] HSP 1 e-value: 8.0E-22 bit: 102.0 Len: 681 Query Start:62 Query End:742 Subject Strand: null Subject Start: 1 Subject End: 227 ............................................................. I R L N K F L S N A G V C S R R E A D L L I E K G E I T V N G E V V T E L G R R I T R Q D E V I Y K D K R V - E I E S K V Y V L L N K P K N C V T T S D D P E C R T T V M D I V R Y A C P E R I Y P V G R L D R N T T G V L L I T N D G D L A S K L T H P S F K K K K I Y H V W L N K D V S I E D M Q K I A D G V E L - D D G E I H A D A I S Y V K E D D L S Q V G I E I H S G R N R I V R R L F E H L G Y R V V K L D R V Y F A G L T - K K N L P R G K W R Y L N E Q E V ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ............................................................. M R L D K F I A Q Q L G V S R A I A G R E I R G N R V T V D G E I V R N A A F K L L P E H D V A Y D G N P L A Q Q H G P R Y F M L N K P Q G Y V C S T D D P D - H P T V L Y F L D E P V A W K L H A A G R L D I D T T G L V L M T D D G Q W S H R I T S P R H H C E K T Y L V T L E S P V A D D T A E Q F A K G V Q L H N E K D L T K P A V L E V I T P - - T Q V R L T I S E G R Y H Q V K R M F A A V G N H V V E L H R E R I G G I T L D A D L A P G E Y R P L T E E E I ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: gi|89107981|ref|AP_001761.1| 23S rRNA pseudouridine synthase [Escherichia coli W3110] HSP 1 e-value: 5.0E-16 bit: 82.8 Len: 489 Query Start:71 Query End:559 Subject Strand: null Subject Start: 42 Subject End: 217 ...................................................................... V L L N K P K N C V T T S D D P E C R T T V M D I V R Y A C P E R I Y P V G R L D R N T T G V L L I T N D G D L A S K L T H P S F K K K K I Y H V W L N K D V S I E D M Q K I A D G V E L D D G E I H A D A I S Y V K E D - - - - - - - - - - - - - - - - D L S Q V G I E I H S G R N R I V R R L F E H L G Y R V V K L D R V Y F A G L T K K N L P R G K W R Y L N E ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ ...................................................................... I L F N K P Y D V L P Q F T D E A G R K T L K E F I P V - - - Q G V Y A A G R L D R D S E G L L V L T N N G A L Q A R L T Q P G K R T G K I Y Y V Q V E G I P T Q D A L E A L R N G V T L N D G P T L P A G A E L V D E P A W L W P R N P P I R E R K S I P T S W L K I T L Y E G R N R Q V R R M T A H V G F P T L R L I R Y A M G D Y S L D N L A N G E W R E V T D ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Find nr database================================================ Query_seq: PG1121:PG1123|PG1121:PG1123:asparaginyl-tRNA synthetase:adenylosuccinate lyase:<-<-:1197646..1199096 1451 tatataatggtattcattatttgtgtttatattattcgaaagctcccatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatcggccagtacctcacgatactttactggcacaatcggagctttcgactgtttctttttcttcttttgctgtggaagtgtcagtgttctattgccacccgaatacatcggcctttccgtatcccgaaactgacggggataagatggtcccgaaccacgtatacgctcgctgtccatagcatgattggcatcgtactctttctcaaaatgtctgagagaatcgcgtctctgcccacgctcttcattttggcggtatgaagccggataagcagcgcgctgcccttcccgactatatcgattgttgtacgaaggagcttcagagcggttatttccttcccctccggagaaatcatcacgtctgtacgattgtgatcgactgctctgctgctcaccgatacgtactcgtcgtttacgctgttgcaattgatccgtcggttcgggtttggatgtagcccctactctgggagtaggtctgaaacctccgcctgaactcattcttgacgaacggaaaggcgtaggtttttctatgtgcttttccgaaggagaaatatcatccttgctcgtatctgcgaaaatatcgcgttcttcagttgtcatctttgatagattattgcttagattttacacatacaacctcacggttgcaaactatggatttgtcttttttgactctttgggttacgaaagtatgaaaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tatataatggtattcattatttgtgtttatattattcgaaagctcccatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatcggccagtacctcacgatactttactggcacaatcggagctttcgactgtttctttttcttcttttgctgtggaagtgtcagtgttctattgccacccgaatacatcggcctttccgtatcccgaaactgacggggataagatggtcccgaaccacgtatacgctcgctgtccatagcatgattggcatcgtactctttctcaaaatgtctgagagaatcgcgtctctgcccacgctcttcattttggcggtatgaagccggataagcagcgcgctgcccttcccgactatatcgattgttgtacgaaggagcttcagagcggttatttccttcccctccggagaaatcatcacgtctgtacgattgtgatcgactgctctgctgctcaccgatacgtactcgtcgtttacgctgttgcaattgatccgtcggttcgggtttggatgtagcccctactctgggagtaggtctgaaacctccgcctgaactcattcttgacgaacggaaaggcgtaggtttttctatgtgcttttccgaaggagaaatatcatccttgctcgtatctgcgaaaatatcgcgttcttcagttgtcatctttgatagattattgcttagattttacacatacaacctcacggttgcaaactatggatttgtcttttttgactctttgggttacgaaagtatgaaaatt Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 249 End: 347 ........................................................................................................................................................................................................................................................ M M A S A C I S P S S N S T P S A I F C M S S M L T S L L S H T W ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 351 End: 560 .............................................................................................................................................................................................................................................................................................................................................................. M F F F L K D G W V S L E A R S P S F V I R S T P V V F R S K R P T G Y I L S G Q A Y R T I S I T V V R H S G S S E V V ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 621 End: 884 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M L L P S S V T T S P L T V I S P F S I S R S A S L R E Q T P A L L K N L F S L I G S F G S A S T S R Y F T G T I G A F ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 50 Strand: + Start: 781 End: 930 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M A Q S E L S T V S F S S F A V E V S V F Y C H P N T S A F P Y P E T D G D K M V P N H V Y A R C P ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 23 End: 1009 ...................... M F I L F E S S H S K V I Y L L F I Q I A P F S T G Q I L L G Q S G K I N A I Q F D N T I A K V F K Q T A H N T I S S A .......................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 35 Strand: + Start: 1019 End: 1123 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M S S A L P F P T I S I V V R R S F R A V I S F P S G E I I T S V R L ........................................................................................................................................................................................................................................................................................................................................ Protein_Len: 42 Strand: + Start: 1053 End: 1178 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M L Y E G A S E R L F P S P P E K S S R L Y D C D R L L C C S P I R T R R L R C C N ................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 1009 End: 1203 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M K P D K Q R A A L P D Y I D C C T K E L Q S G Y F L P L R R N H H V C T I V I D C S A A H R Y V L V V Y A V A I D P S ........................................................................................................................................................................................................................................................ Protein_Len: 35 Strand: + Start: 1136 End: 1240 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M L L T D T Y S S F T L L Q L I R R F G F G C S P Y S G S R S E T S A ................................................................................................................................................................................................................... Protein_Len: 41 Strand: + Start: 1321 End: 1443 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M R K Y R V L Q L S S L I D Y C L D F T H T T S R L Q T M D L S F L T L W V T K V ........ Protein_Len: 60 Strand: + Start: 1197 End: 1451 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M D V A P T L G V G L K P P P E L I L D E R K G V G F S M C F S E G E I S S L L V S A K I S R S S V V I F D R L L L R F Protein_Len: 60 Strand: - Start: 838 End: 1182 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... A T H L R I L L A S G K G V I D I T T R L L K L A T I E K G E P S I M V D T R N H D V A R S S V S V Y E D N V S N C N M ............................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 35 End: 1351 .................................. Q L Q D T P E P K S T A G V R P T P R F G G G S S M R S S R F P T P K E I H K E S P S I D D K S T D A F I D R E E T T M .................................................................................................... tatataatggtattcattatttgtgtttatattattcgaaagctcccatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatcggccagtacctcacgatactttactggcacaatcggagctttcgactgtttctttttcttcttttgctgtggaagtgtcagtgttctattgccacccgaatacatcggcctttccgtatcccgaaactgacggggataagatggtcccgaaccacgtatacgctcgctgtccatagcatgattggcatcgtactctttctcaaaatgtctgagagaatcgcgtctctgcccacgctcttcattttggcggtatgaagccggataagcagcgcgctgcccttcccgactatatcgattgttgtacgaaggagcttcagagcggttatttccttcccctccggagaaatcatcacgtctgtacgattgtgatcgactgctctgctgctcaccgatacgtactcgtcgtttacgctgttgcaattgatccgtcggttcgggtttggatgtagcccctactctgggagtaggtctgaaacctccgcctgaactcattcttgacgaacggaaaggcgtaggtttttctatgtgcttttccgaaggagaaatatcatccttgctcgtatctgcgaaaatatcgcgttcttcagttgtcatctttgatagattattgcttagattttacacatacaacctcacggttgcaaactatggatttgtcttttttgactctttgggttacgaaagtatgaaaatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 82 Start: 1373 End: 1402 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTGCAACCGTGAGGTTGTATGTGTAAAAT................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tatataatggtattcattatttgtgtttatattattcgaaagctcccatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatcggccagtacctcacgatactttactggcacaatcggagctttcgactgtttctttttcttcttttgctgtggaagtgtcagtgttctattgccacccgaatacatcggcctttccgtatcccgaaactgacggggataagatggtcccgaaccacgtatacgctcgctgtccatagcatgattggcatcgtactctttctcaaaatgtctgagagaatcgcgtctctgcccacgctcttcattttggcggtatgaagccggataagcagcgcgctgcccttcccgactatatcgattgttgtacgaaggagcttcagagcggttatttccttcccctccggagaaatcatcacgtctgtacgattgtgatcgactgctctgctgctcaccgatacgtactcgtcgtttacgctgttgcaattgatccgtcggttcgggtttggatgtagcccctactctgggagtaggtctgaaacctccgcctgaactcattcttgacgaacggaaaggcgtaggtttttctatgtgcttttccgaaggagaaatatcatccttgctcgtatctgcgaaaatatcgcgttcttcagttgtcatctttgatagattattgcttagattttacacatacaacctcacggttgcaaactatggatttgtcttttttgactctttgggttacgaaagtatgaaaatt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 79 HP_score: -2.2 Tail_Score: -5.4593 Start: 783 End: 809 Full_Region: TCACGATACTTTACT GGCACAATCGGA GCT TTCGACTGTTTC TTTTTCTTCTTTTGC ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GGCACAATCGGAGCTTTCGACTGTTTC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 77 HP_score: -3.8 Tail_Score: -5.11254 Start: 787 End: 805 Full_Region: GATACTTTACTGGCA CAATCGGA GCT TTCGACTG TTTCTTTTTCTTCTT ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CAATCGGAGCTTTCGACTG...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: PG1121:PG1123|PG1121:PG1123:asparaginyl-tRNA synthetase:adenylosuccinate lyase:<-<-:1197646..1199096 1451 tatataatggtattcattatttgtgtttatattattcgaaagctcccatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatcggccagtacctcacgatactttactggcacaatcggagctttcgactgtttctttttcttcttttgctgtggaagtgtcagtgttctattgccacccgaatacatcggcctttccgtatcccgaaactgacggggataagatggtcccgaaccacgtatacgctcgctgtccatagcatgattggcatcgtactctttctcaaaatgtctgagagaatcgcgtctctgcccacgctcttcattttggcggtatgaagccggataagcagcgcgctgcccttcccgactatatcgattgttgtacgaaggagcttcagagcggttatttccttcccctccggagaaatcatcacgtctgtacgattgtgatcgactgctctgctgctcaccgatacgtactcgtcgtttacgctgttgcaattgatccgtcggttcgggtttggatgtagcccctactctgggagtaggtctgaaacctccgcctgaactcattcttgacgaacggaaaggcgtaggtttttctatgtgcttttccgaaggagaaatatcatccttgctcgtatctgcgaaaatatcgcgttcttcagttgtcatctttgatagattattgcttagattttacacatacaacctcacggttgcaaactatggatttgtcttttttgactctttgggttacgaaagtatgaaaatt Intra-Species Hit: Count: 1 Min: 1 Max: 1451 Len: 1451 Subject: NC_002950_PG1121_PG1123|asparaginyl-tRNA synthetase:adenylosuccinate lyase|NEGATIVE:NEGATIVE|[1197646,1199096]|1451 HSP 1 e-value: 0.0 bit: 1382.0 Len: 697 Query Start:755 Query End:1451 Subject Strand: POSITIVE Subject Start: 755 Subject End: 1451 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................atcggccagtacctcacgatactttactggcacaatcggagctttcgactgtttctttttcttcttttgctgtggaagtgtcagtgttctattgccacccgaatacatcggcctttccgtatcccgaaactgacggggataagatggtcccgaaccacgtatacgctcgctgtccatagcatgattggcatcgtactctttctcaaaatgtctgagagaatcgcgtctctgcccacgctcttcattttggcggtatgaagccggataagcagcgcgctgcccttcccgactatatcgattgttgtacgaaggagcttcagagcggttatttccttcccctccggagaaatcatcacgtctgtacgattgtgatcgactgctctgctgctcaccgatacgtactcgtcgtttacgctgttgcaattgatccgtcggttcgggtttggatgtagcccctactctgggagtaggtctgaaacctccgcctgaactcattcttgacgaacggaaaggcgtaggtttttctatgtgcttttccgaaggagaaatatcatccttgctcgtatctgcgaaaatatcgcgttcttcagttgtcatctttgatagattattgcttagattttacacatacaacctcacggttgcaaactatggatttgtcttttttgactctttgggttacgaaagtatgaaaatt ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................atcggccagtacctcacgatactttactggcacaatcggagctttcgactgtttctttttcttcttttgctgtggaagtgtcagtgttctattgccacccgaatacatcggcctttccgtatcccgaaactgacggggataagatggtcccgaaccacgtatacgctcgctgtccatagcatgattggcatcgtactctttctcaaaatgtctgagagaatcgcgtctctgcccacgctcttcattttggcggtatgaagccggataagcagcgcgctgcccttcccgactatatcgattgttgtacgaaggagcttcagagcggttatttccttcccctccggagaaatcatcacgtctgtacgattgtgatcgactgctctgctgctcaccgatacgtactcgtcgtttacgctgttgcaattgatccgtcggttcgggtttggatgtagcccctactctgggagtaggtctgaaacctccgcctgaactcattcttgacgaacggaaaggcgtaggtttttctatgtgcttttccgaaggagaaatatcatccttgctcgtatctgcgaaaatatcgcgttcttcagttgtcatctttgatagattattgcttagattttacacatacaacctcacggttgcaaactatggatttgtcttttttgactctttgggttacgaaagtatgaaaatt HSP 2 e-value: 1.0E-18 bit: 97.6 Len: 49 Query Start:1 Query End:49 Subject Strand: POSITIVE Subject Start: 1 Subject End: 49 tatataatggtattcattatttgtgtttatattattcgaaagctcccat.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... tatataatggtattcattatttgtgtttatattattcgaaagctcccat.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUAUAAUGGUAUUCAUUAUUUGUGUUUAUAUUAUUCGAAAGCUCCCAUUCUAAGGUUAUUUACCUCCUGUUCAUUCAGAUAGCGCCAUUUUCCACGGGGCAGAUUCUUCUUGGUCAGUCCGGCAAAAUAAACGCGAUCCAGUUUGACAACACGAUAGCCAAGGUGUUCAAACAAACGGCGCACAAUACGAUUUCGUCCGCUAUGAAUCUCUAUUCCCACCUGCGAAAGAUCGUCUUCUUUUACGUAACUGAUGGCAUCGGCAUGUAUCUCACCAUCAUCCAAUUCCACGCCGUCGGCUAUUUUCUGCAUGUCUUCAAUGCUUACAUCUUUAUUGAGCCACACGUGGUAGAUCUUCUUCUUCUUGAAAGACGGAUGGGUCAGCUUAGAGGCAAGAUCGCCAUCAUUUGUAAUCAGAAGUACACCGGUAGUAUUCCGAUCCAAACGUCCUACCGGAUAUAUUCUCUCAGGACAGGCAUAUCGUACUAUAUCCAUUACUGUGGUACGGCAUUCGGGGUCAUCAGAAGUGGUCACACAAUUUUUAGGCUUAUUGAGCAGUACAUAAACUUUACUCUCUAUUUCCACUCUCUUAUCCUUGUAGAUUACCUCAUCCUGACGUGUAAUUCUUCUACCGAGUUCGGUCACGACUUCUCCAUUGACCGUGAUCUCUCCCUUUUCGAUCAGCAGAUCCGCUUCCCUUCGCGAGCAAACACCGGCAUUGCUCAAGAACUUGUUCAGUCUGAUCGGCUCAUUCGGAUCGGCCAGUACCUCACGAUACUUUACUGGCACAAUCGGAGCUUUCGACUGUUUCUUUUUCUUCUUUUGCUGUGGAAGUGUCAGUGUUCUAUUGCCACCCGAAUACAUCGGCCUUUCCGUAUCCCGAAACUGACGGGGAUAAGAUGGUCCCGAACCACGUAUACGCUCGCUGUCCAUAGCAUGAUUGGCAUCGUACUCUUUCUCAAAAUGUCUGAGAGAAUCGCGUCUCUGCCCACGCUCUUCAUUUUGGCGGUAUGAAGCCGGAUAAGCAGCGCGCUGCCCUUCCCGACUAUAUCGAUUGUUGUACGAAGGAGCUUCAGAGCGGUUAUUUCCUUCCCCUCCGGAGAAAUCAUCACGUCUGUACGAUUGUGAUCGACUGCUCUGCUGCUCACCGAUACGUACUCGUCGUUUACGCUGUUGCAAUUGAUCCGUCGGUUCGGGUUUGGAUGUAGCCCCUACUCUGGGAGUAGGUCUGAAACCUCCGCCUGAACUCAUUCUUGACGAACGGAAAGGCGUAGGUUUUUCUAUGUGCUUUUCCGAAGGAGAAAUAUCAUCCUUGCUCGUAUCUGCGAAAAUAUCGCGUUCUUCAGUUGUCAUCUUUGAUAGAUUAUUGCUUAGAUUUUACACAUACAACCUCACGGUUGCAAACUAUGGAUUUGUCUUUUUUGACUCUUUGGGUUACGAAAGUAUGAAAAUU ...((((((.....)))))).....((((((((.((((...((.(((......((((.....)))).(((......))).................))))).(((((.....((((((...(((((....((((((((...((((((..((((..........))))))))))......((((..((((((..(((((....((((.............((((.((......(((((((.(((((....((((((((((.((.(.((.((.(((..(((((....(((((..((.((((.......((((.......))))..............)))))).)))))....(((((........).)))).)))))))).))..)).).))..))).)))))))..)))))..))))).)).(((((((......(((......))))))))))........)).)))).(((((........(((((((((........(((((...((((((..((((....(((....)))........((...((.(((.((((.........))))))).))..)).......(((((((((.(((((((((.....)).).))))))........(((...(((((((...((......)).))))))).)))...(((((..(((((((...............((((((.........)))))).((((...(..(((((.((..(((((.....(((.((((((((.............))))))).).))).))))).)))))))...)...))))...))))))).(((((.(((.((((((...........))))))...))).)))))......)))))...))))))))).))))..))))))...)))))..((.((((((.(((...))).(((((.(((((((.....((((((((...(((((....(((....))).....))))))))))))).))))))).)))))....)))))).)).((((...(((((...(((((((((..((((...((((..((((((((((.(((((.........)))))....((((((((.....)).)))))).))))))))))..))))..((((........))))....)).))..)))))))))..)))))...)))).)))))))))..(((((((...)))))))............)))))...))))....))))).((((((((((((((....)))))..)))))))))(((((((....)).)))))..)))))).)))).....))))))))......((((((....))))))....))))).((((......(((((((((....))))).....)))).....))))...))))))....))))).)))))))))))).... (-405.02) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUUUCAUACUUUCGUAACCCAAAGAGUCAAAAAAGACAAAUCCAUAGUUUGCAACCGUGAGGUUGUAUGUGUAAAAUCUAAGCAAUAAUCUAUCAAAGAUGACAACUGAAGAACGCGAUAUUUUCGCAGAUACGAGCAAGGAUGAUAUUUCUCCUUCGGAAAAGCACAUAGAAAAACCUACGCCUUUCCGUUCGUCAAGAAUGAGUUCAGGCGGAGGUUUCAGACCUACUCCCAGAGUAGGGGCUACAUCCAAACCCGAACCGACGGAUCAAUUGCAACAGCGUAAACGACGAGUACGUAUCGGUGAGCAGCAGAGCAGUCGAUCACAAUCGUACAGACGUGAUGAUUUCUCCGGAGGGGAAGGAAAUAACCGCUCUGAAGCUCCUUCGUACAACAAUCGAUAUAGUCGGGAAGGGCAGCGCGCUGCUUAUCCGGCUUCAUACCGCCAAAAUGAAGAGCGUGGGCAGAGACGCGAUUCUCUCAGACAUUUUGAGAAAGAGUACGAUGCCAAUCAUGCUAUGGACAGCGAGCGUAUACGUGGUUCGGGACCAUCUUAUCCCCGUCAGUUUCGGGAUACGGAAAGGCCGAUGUAUUCGGGUGGCAAUAGAACACUGACACUUCCACAGCAAAAGAAGAAAAAGAAACAGUCGAAAGCUCCGAUUGUGCCAGUAAAGUAUCGUGAGGUACUGGCCGAUCCGAAUGAGCCGAUCAGACUGAACAAGUUCUUGAGCAAUGCCGGUGUUUGCUCGCGAAGGGAAGCGGAUCUGCUGAUCGAAAAGGGAGAGAUCACGGUCAAUGGAGAAGUCGUGACCGAACUCGGUAGAAGAAUUACACGUCAGGAUGAGGUAAUCUACAAGGAUAAGAGAGUGGAAAUAGAGAGUAAAGUUUAUGUACUGCUCAAUAAGCCUAAAAAUUGUGUGACCACUUCUGAUGACCCCGAAUGCCGUACCACAGUAAUGGAUAUAGUACGAUAUGCCUGUCCUGAGAGAAUAUAUCCGGUAGGACGUUUGGAUCGGAAUACUACCGGUGUACUUCUGAUUACAAAUGAUGGCGAUCUUGCCUCUAAGCUGACCCAUCCGUCUUUCAAGAAGAAGAAGAUCUACCACGUGUGGCUCAAUAAAGAUGUAAGCAUUGAAGACAUGCAGAAAAUAGCCGACGGCGUGGAAUUGGAUGAUGGUGAGAUACAUGCCGAUGCCAUCAGUUACGUAAAAGAAGACGAUCUUUCGCAGGUGGGAAUAGAGAUUCAUAGCGGACGAAAUCGUAUUGUGCGCCGUUUGUUUGAACACCUUGGCUAUCGUGUUGUCAAACUGGAUCGCGUUUAUUUUGCCGGACUGACCAAGAAGAAUCUGCCCCGUGGAAAAUGGCGCUAUCUGAAUGAACAGGAGGUAAAUAACCUUAGAAUGGGAGCUUUCGAAUAAUAUAAACACAAAUAAUGAAUACCAUUAUAUA ........(((((((...(((......(((......)))..((((......(((((((....))))))).((((((..(((.((.....((((.....)))).....))..)))..((((.....)))).....((((..........((((((((((.(((..(((........((((((.(((((..((((((.....))))).)...))))).))))))....(((((((...))))))).)))...)))...(((...(((((..(((.((((......(((.....))).(((((....((.((((..((((((.(((.(((((..((.....)).))))))))((((((...))))))..........))))))..)))))).)))))..)))).)))...)))))...)))(((((..((((((.((((((........))).........((((((((((......(((((((((((((...)))))))..)))).)).))))...)))))).......(((((((.((((.((((..((((........))))..((((((((((....(((.....)))...((((((((((((........((((...((((..........))))........(((((((.......)))))))..))))..((((((....)))))).))).)))))))))..))))...))))))....(((....)))...)))))))).)))))))...)))))))))...))))).......))))))))))..((((((.(((.....)))))))))..)))).........))))))....))))..)))..(((((............)))))....)))))))..((((((((...(((.....))).......(((.((..(((.((((((.(.....((((((.((....((((....((((...(((((((.(((......((((((......(((((((..((.......)).....))))))).((.(((((..((((...(((((((.(((..((.......((.((((((((.(((((......))))).......((((((.(((((..(((....)))..((((.......)))).......)))))...))))))....)))))..))).))......)).)))))))))).....)))).)))))))..))))))..(((((.((((....))))....((((((((..(((.....)))...))))))))..))))).)))))))))).))))..)))).)).))))))..((((((...(((..((...(((....((.((((.....)))).))..)))...))..)))..))))))...).)))))))))..))...)))...))))))))....((((((.....))))))... (-433.42) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1004 140 91 231 182 NC_002950:PG1121|5end_asparaginyl-tRNA_synthetase_1197555..1197785_NEGATIVE 491 317 271 77 15 NC_002950:PG1521|5end_O-succinylbenzoic_acid--CoA_ligase_1599103..1599333_NEGATIVE 456 1199 1152 53 11 NC_002950:PG1675|5end_hypothetical_protein_1760182..1760412_POSITIVE 434 1030 985 188 136 NC_002950:PG2187|5end_1,4-dihydroxy-2-naphthoate_octaprenyltransferase_2294643..2294873_NEGATIVE 432 1050 1004 50 11 NC_002950:PG1639|5end_hypothetical_protein_1719919..1720149_NEGATIVE 429 706 662 51 1 NC_002950:PG0738|5end_cytidine/deoxycytidylate_deaminase_family_protein_786463..786693_POSITIVE 411 122 84 57 14 NC_002950:PG0253|5end_hypothetical_protein_284607..284837_POSITIVE 410 719 671 229 174 NC_002950:PG1498|5end_hypothetical_protein_1574656..1574886_POSITIVE 410 1043 1001 69 24 NC_002950:PG0802|5end_alpha_keto_acid_dehydrogenase_complex,_E3_component,_lipoamide_dehydrogenase_859722..859952_NEGATIVE 409 705 661 57 1 NC_002950:PG1728|5end_cytidine/deoxycytidylate_deaminase_family_protein_1817644..1817874_POSITIVE 402 1047 1001 185 121 NC_002950:PG0423|5end_hypothetical_protein_462251..462481_POSITIVE 397 1171 1132 173 133 NC_002950:PG1370|5end_lysyl-tRNA_synthetase_1452229..1452459_NEGATIVE 394 720 671 53 3 NC_002950:PG1583|5end_batB_protein_1660315..1660545_POSITIVE 393 1230 1183 222 155 NC_002950:PG0211|5end_cobalamin_biosynthesis_protein_CbiG/precorrin-4_C11-methyltransferase_247810..248040_NEGATIVE 392 1017 977 231 189 NC_002950:PG1945|5end_hypothetical_protein_2031697..2031927_POSITIVE 391 721 684 170 126 NC_002950:PG2080|5end_adenosylmethionine--8-amino-7-oxononanoate_transaminase_2180679..2180909_NEGATIVE 391 902 865 190 154 NC_002950:PG1093|5end_hypothetical_protein_1158121..1158351_POSITIVE 390 1042 1002 217 167 NC_002950:PG1815|5end_3-deoxy-manno-octulosonate_cytidylyltransferase_1911433..1911663_NEGATIVE 390 1257 1213 62 4 NC_002950:PG0790|5end_GTP-binding_protein_Obg_842583..842813_NEGATIVE 389 722 682 56 24 NC_002950:PG1992|5end_glucose-inhibited_division_protein_A_2081410..2081640_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 491 317 271 80 18 NC_002950:PG1522|3end_mandelate_racemase/muconate_lactonizing_enzyme_family_protein_1599100..1599250_NEGATIVE 456 1199 1152 151 109 NC_002950:PG1675|3end_hypothetical_protein_1760360..1760510_POSITIVE 429 706 662 54 4 NC_002950:PG0737|3end_hypothetical_protein_786546..786696_POSITIVE 410 719 671 128 73 NC_002950:PG1497|3end_DNA-binding_protein,_histone-like_family_1574635..1574785_POSITIVE 410 1043 1001 64 19 NC_002950:PG0803|3end_glucosamine-6-phosphate_deaminase_859727..859877_NEGATIVE 408 890 842 131 84 NC_002950:PG0068|3end_hypothetical_protein_82079..82229_POSITIVE 397 1171 1132 50 10 NC_002950:PG1371|3end_phosphorylase_family_protein_1452352..1452502_NEGATIVE 396 760 711 146 96 NC_002950:PG0162|3end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_184505..184655_NEGATIVE 391 902 865 81 45 NC_002950:PG1091|3end_DHH_subfamily_1_protein_1158092..1158242_POSITIVE 386 907 863 142 96 NC_002950:PG1105|3end_RNA_polymerase_sigma-54_factor_1175716..1175866_NEGATIVE 386 1194 1155 137 97 NC_002950:PG0443|3end_hemagglutinin-related_protein_483147..483297_POSITIVE 384 1230 1181 72 21 NC_002950:PG2124|3end_glyceraldehyde_3-phosphate_dehydrogenase,_type_I_2231264..2231414_POSITIVE 380 1059 1014 94 44 NC_002950:PG1613|3end_glyoxalase_family_protein_1693164..1693314_NEGATIVE 377 1047 1002 94 50 NC_002950:PG1270|3end_hypothetical_protein_1349278..1349428_NEGATIVE 377 769 728 141 90 NC_002950:PG0152|3end_carboxynorspermidine_decarboxylase_174521..174671_NEGATIVE 373 1159 1125 133 96 NC_002950:PG2040|3end_DNA-binding_protein,_histone-like_family_2138398..2138548_NEGATIVE 373 1029 983 66 19 NC_002950:PG0296|3end_phosphoribosylformylglycinamidine_synthase_331293..331443_NEGATIVE 371 120 74 144 94 NC_002950:PG2142|3end_GTP-binding_protein_Era_2248564..2248714_POSITIVE 368 759 711 78 14 NC_002950:PG2158|3end_hypothetical_protein_2264473..2264623_POSITIVE 367 1030 981 84 21 NC_002950:PG2224|3end_hypothetical_protein_2339500..2339650_POSITIVE