Origin IGS: taagcatcaactgttcgtagaccacgtatattctactatttgttctttaacattggtgttgagcaagtaatgtcaagaagttttccgagacgtattgcctaggattgtcaaacctgattgtcccaaagctcagtgaagtcggctcttacgtgtcatagatcgtttacctttgtcactgtatgaaccacagacgatcaaaaacc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ggtttttgatcgtctgtggttcatacagtgacaaaggtaaacgatctatgacacgtaagagccgacttcactgagctttgggacaatcaggtttgacaatcctaggcaatacgtctcggaaaacttcttgacattacttgctcaacaccaatgttaaagaacaaatagtagaatatacgtggtctacgaacagttgatgctta Mask Tandem Repeat Region ================================================ taagcatcaactgttcgtagaccacgtatattctactatttgttctttaacattggtgttgagcaagtaatgtcaagaagttttccgagacgtattgcctaggattgtcaaacctgattgtcccaaagctcagtgaagtcggctcttacgtgtcatagatcgtttacctttgtcactgtatgaaccacagacgatcaaaaacc Find is-nt database================================================ Query_seq: PG0972:PG0973|PG0972:PG0973:hypothetical protein:alpha-1,2-mannosidase family protein:->->:1034104..1034306 203 taagcatcaactgttcgtagaccacgtatattctactatttgttctttaacattggtgttgagcaagtaatgtcaagaagttttccgagacgtattgcctaggattgtcaaacctgattgtcccaaagctcagtgaagtcggctcttacgtgtcatagatcgtttacctttgtcactgtatgaaccacagacgatcaaaaacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0972:PG0973|PG0972:PG0973:hypothetical protein:alpha-1,2-mannosidase family protein:->->:1034104..1034306 203 taagcatcaactgttcgtagaccacgtatattctactatttgttctttaacattggtgttgagcaagtaatgtcaagaagttttccgagacgtattgcctaggattgtcaaacctgattgtcccaaagctcagtgaagtcggctcttacgtgtcatagatcgtttacctttgtcactgtatgaaccacagacgatcaaaaacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0972:PG0973|PG0972:PG0973:hypothetical protein:alpha-1,2-mannosidase family protein:->->:1034104..1034306 203 taagcatcaactgttcgtagaccacgtatattctactatttgttctttaacattggtgttgagcaagtaatgtcaagaagttttccgagacgtattgcctaggattgtcaaacctgattgtcccaaagctcagtgaagtcggctcttacgtgtcatagatcgtttacctttgtcactgtatgaaccacagacgatcaaaaacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagcatcaactgttcgtagaccacgtatattctactatttgttctttaacattggtgttgagcaagtaatgtcaagaagttttccgagacgtattgcctaggattgtcaaacctgattgtcccaaagctcagtgaagtcggctcttacgtgtcatagatcgtttacctttgtcactgtatgaaccacagacgatcaaaaacc Predict ORF larger than 30AA ================================================ taagcatcaactgttcgtagaccacgtatattctactatttgttctttaacattggtgttgagcaagtaatgtcaagaagttttccgagacgtattgcctaggattgtcaaacctgattgtcccaaagctcagtgaagtcggctcttacgtgtcatagatcgtttacctttgtcactgtatgaaccacagacgatcaaaaacc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagcatcaactgttcgtagaccacgtatattctactatttgttctttaacattggtgttgagcaagtaatgtcaagaagttttccgagacgtattgcctaggattgtcaaacctgattgtcccaaagctcagtgaagtcggctcttacgtgtcatagatcgtttacctttgtcactgtatgaaccacagacgatcaaaaacc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0972:PG0973|PG0972:PG0973:hypothetical protein:alpha-1,2-mannosidase family protein:->->:1034104..1034306 203 taagcatcaactgttcgtagaccacgtatattctactatttgttctttaacattggtgttgagcaagtaatgtcaagaagttttccgagacgtattgcctaggattgtcaaacctgattgtcccaaagctcagtgaagtcggctcttacgtgtcatagatcgtttacctttgtcactgtatgaaccacagacgatcaaaaacc Intra-Species Hit: Count: 1 Min: 1 Max: 203 Len: 203 Subject: NC_002950_PG0972_PG0973|hypothetical protein:alpha-1,2-mannosidase family protein|POSITIVE:POSITIVE|[1034104,1034306]|203 HSP 1 e-value: 1.0E-111 bit: 402.0 Len: 203 Query Start:1 Query End:203 Subject Strand: POSITIVE Subject Start: 1 Subject End: 203 taagcatcaactgttcgtagaccacgtatattctactatttgttctttaacattggtgttgagcaagtaatgtcaagaagttttccgagacgtattgcctaggattgtcaaacctgattgtcccaaagctcagtgaagtcggctcttacgtgtcatagatcgtttacctttgtcactgtatgaaccacagacgatcaaaaacc taagcatcaactgttcgtagaccacgtatattctactatttgttctttaacattggtgttgagcaagtaatgtcaagaagttttccgagacgtattgcctaggattgtcaaacctgattgtcccaaagctcagtgaagtcggctcttacgtgtcatagatcgtttacctttgtcactgtatgaaccacagacgatcaaaaacc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGCAUCAACUGUUCGUAGACCACGUAUAUUCUACUAUUUGUUCUUUAACAUUGGUGUUGAGCAAGUAAUGUCAAGAAGUUUUCCGAGACGUAUUGCCUAGGAUUGUCAAACCUGAUUGUCCCAAAGCUCAGUGAAGUCGGCUCUUACGUGUCAUAGAUCGUUUACCUUUGUCACUGUAUGAACCACAGACGAUCAAAAACC ..((((((((.((((...(((.((.(((........))).)).)))..))))))))))))...................(((((..((.(((((..(((..((((.((((....)))).))))....(((......))).)))...))))).))...((((((((.......(((.....))).....)))))))).))))). (-43.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUUUUUGAUCGUCUGUGGUUCAUACAGUGACAAAGGUAAACGAUCUAUGACACGUAAGAGCCGACUUCACUGAGCUUUGGGACAAUCAGGUUUGACAAUCCUAGGCAAUACGUCUCGGAAAACUUCUUGACAUUACUUGCUCAACACCAAUGUUAAAGAACAAAUAGUAGAAUAUACGUGGUCUACGAACAGUUGAUGCUUA (((...(((..(((...(((((.(((.(((.((.((((.....)))).)).)))))).)))))))).))).(((((...((.....(((((........(((.((((.....)))).)))......))))).....)).)))))..)))..((((..(((.((....(((.....))).)).)))...))))........... (-43.94) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 347 104 61 103 58 NC_002950:PG1773|5end_PAP2_superfamily_protein_1859815..1860045_POSITIVE 335 109 64 150 89 NC_002950:PG1026|5end_hypothetical_protein_1088177..1088407_POSITIVE 327 158 112 120 72 NC_002950:PG0026|5end_hypothetical_protein_29234..29464_POSITIVE 318 103 61 170 130 NC_002950:PG1904|5end_hypothetical_protein_2004761..2004991_NEGATIVE 317 140 91 182 117 NC_002950:PG0148|5end_sigma-54-dependent_transcriptional_regulator_170907..171137_NEGATIVE 316 130 82 196 132 NC_002950:PG1098|5end_hypothetical_protein_1167009..1167239_NEGATIVE 312 158 114 219 168 NC_002950:PG2075|5end_hypothetical_protein_2176466..2176696_NEGATIVE 312 169 127 182 128 NC_002950:PG0678|5end_pyrazinamidase/nicotinamidase,_putative_728879..729109_POSITIVE 309 130 82 110 54 NC_002950:PG2189|5end_aspartate_kinase_2297151..2297381_NEGATIVE 304 185 141 216 174 NC_002950:PG1310|5end_exsB_protein_1389084..1389314_POSITIVE 304 163 127 120 71 NC_002950:PG0039|5end_hypothetical_protein_47637..47867_POSITIVE 300 158 113 223 180 NC_002950:PG1640|5end_DNA-damage-inducible_protein_F_1721245..1721475_NEGATIVE 300 152 117 52 14 NC_002950:PG0927|5end_conserved_hypothetical_protein_TIGR00150_985050..985280_NEGATIVE 297 165 121 53 4 NC_002950:PG0880|5end_bacterioferritin_comigratory_protein_942539..942769_POSITIVE 296 144 102 204 164 NC_002950:PG1947|5end_TPR_domain_protein_2032932..2033162_POSITIVE 296 110 63 196 142 NC_002950:PG0962|5end_prolyl-tRNA_synthetase_1023808..1024038_NEGATIVE 296 150 113 182 145 NC_002950:PG0158|5end_competence_protein_F-related_protein_180175..180405_POSITIVE 295 130 82 220 160 NC_002950:PG1343|5end_lipoate-protein_ligase_B_1423628..1423858_POSITIVE 295 150 101 75 33 NC_002950:PG0785|5end_tonB_protein,_putative_837592..837822_NEGATIVE 294 130 86 191 148 NC_002950:PG2170|5end_sugar_transporter_2277974..2278204_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 392 150 102 127 73 NC_002950:PG0384|3end_MutS2_family_protein_418697..418847_POSITIVE 322 158 114 47 10 NC_002950:PG1121|3end_asparaginyl-tRNA_synthetase_1196146..1196296_NEGATIVE 312 169 121 53 6 NC_002950:PG1005|3end_lipoprotein,_putative_1063023..1063173_NEGATIVE 312 169 127 149 95 NC_002950:PG0677|3end_saccharopine_dehydrogenase_728926..729076_POSITIVE 310 140 92 148 101 NC_002950:PG1943|3end_hypothetical_protein_2028974..2029124_NEGATIVE 309 130 82 92 36 NC_002950:PG2190|3end_cell-division_ATP-binding_protein_2297169..2297319_NEGATIVE 300 152 117 40 2 NC_002950:PG0928|3end_response_regulator_985062..985212_NEGATIVE 298 138 94 130 84 NC_002950:PG0969|3end_S-adenosylmethionine:tRNA_ribosyltransferase-isomerase,_putative_1028201..1028351_NEGATIVE 295 150 101 97 55 NC_002950:PG0786|3end_hypothetical_protein_837570..837720_NEGATIVE 294 104 62 41 1 NC_002950:PG1772|3end_endonuclease_III_1859833..1859983_POSITIVE 293 110 74 128 82 NC_002950:PG0840|3end_hypothetical_protein_902483..902633_POSITIVE 286 158 115 134 88 NC_002950:PG0445|3end_peptidase_T_486549..486699_POSITIVE 284 150 101 89 37 NC_002950:PG1433|3end_hydrolase_1519126..1519276_POSITIVE 284 150 111 140 103 NC_002950:PG0597|3end_ribosomal_protein_L9_654281..654431_POSITIVE 281 150 101 142 87 NC_002950:PG1743|3end_2-dehydro-3-deoxyphosphooctonate_aldolase_1827530..1827680_NEGATIVE 280 180 137 95 47 NC_002950:PG0285|3end_hypothetical_protein_320044..320194_POSITIVE 279 158 118 118 82 NC_002950:PG1315|3end_peptidyl-prolyl_cis-trans_isomerase_SlyD,_FKBP-type_1395618..1395768_NEGATIVE 275 129 86 151 109 NC_002950:PG2171|3end_D-isomer_specific_2-hydroxyacid_dehydrogenase_family_protein_2278013..2278163_NEGATIVE 275 136 92 57 15 NC_002950:PG0271|3end_single-stranded_binding_protein_304392..304542_POSITIVE 271 143 102 141 88 NC_002950:PG1232|3end_glutamate_dehydrogenase_1308274..1308424_NEGATIVE