Origin IGS: tttggccacgttctcccgctcattgtaggtagggataataactatactgtctgtcatcgtgtactttctccgagttcgtttgctgaacatctaatcggcatagccggcactcctttgctacaaagatacgaactttgatatacgcaaacaccttattccaaagcttttgcacatggtcctcattctcgaatgaggcctttcg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cgaaaggcctcattcgagaatgaggaccatgtgcaaaagctttggaataaggtgtttgcgtatatcaaagttcgtatctttgtagcaaaggagtgccggctatgccgattagatgttcagcaaacgaactcggagaaagtacacgatgacagacagtatagttattatccctacctacaatgagcgggagaacgtggccaaa Mask Tandem Repeat Region ================================================ tttggccacgttctcccgctcattgtaggtagggataataactatactgtctgtcatcgtgtactttctccgagttcgtttgctgaacatctaatcggcatagccggcactcctttgctacaaagatacgaactttgatatacgcaaacaccttattccaaagcttttgcacatggtcctcattctcgaatgaggcctttcg Find is-nt database================================================ Query_seq: PG0920:PG0922|PG0920:PG0922:glycosyl transferase, group 2 family protein:hypothetical protein:<-<-:981116..981317 202 tttggccacgttctcccgctcattgtaggtagggataataactatactgtctgtcatcgtgtactttctccgagttcgtttgctgaacatctaatcggcatagccggcactcctttgctacaaagatacgaactttgatatacgcaaacaccttattccaaagcttttgcacatggtcctcattctcgaatgaggcctttcg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0920:PG0922|PG0920:PG0922:glycosyl transferase, group 2 family protein:hypothetical protein:<-<-:981116..981317 202 tttggccacgttctcccgctcattgtaggtagggataataactatactgtctgtcatcgtgtactttctccgagttcgtttgctgaacatctaatcggcatagccggcactcctttgctacaaagatacgaactttgatatacgcaaacaccttattccaaagcttttgcacatggtcctcattctcgaatgaggcctttcg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0920:PG0922|PG0920:PG0922:glycosyl transferase, group 2 family protein:hypothetical protein:<-<-:981116..981317 202 tttggccacgttctcccgctcattgtaggtagggataataactatactgtctgtcatcgtgtactttctccgagttcgtttgctgaacatctaatcggcatagccggcactcctttgctacaaagatacgaactttgatatacgcaaacaccttattccaaagcttttgcacatggtcctcattctcgaatgaggcctttcg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttggccacgttctcccgctcattgtaggtagggataataactatactgtctgtcatcgtgtactttctccgagttcgtttgctgaacatctaatcggcatagccggcactcctttgctacaaagatacgaactttgatatacgcaaacaccttattccaaagcttttgcacatggtcctcattctcgaatgaggcctttcg Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 22 End: 135 ..................... M V G R D N N Y T V C H R V L S P S S F A E H L I G I A G T P L L Q R Y E L ................................................................... Protein_Len: 50 Strand: - Start: 23 End: 172 ...................... Q L Y P Y Y S Y Q R D D H V K E S N T Q Q V D L R C L R C E K A V F I R V K I Y A F V K N W L K Q M .............................. Protein_Len: 30 Strand: - Start: 1 End: 90 K A V N E R E N Y T P I I V I S D T M T Y K R R T R K S F M ................................................................................................................ tttggccacgttctcccgctcattgtaggtagggataataactatactgtctgtcatcgtgtactttctccgagttcgtttgctgaacatctaatcggcatagccggcactcctttgctacaaagatacgaactttgatatacgcaaacaccttattccaaagcttttgcacatggtcctcattctcgaatgaggcctttcg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttggccacgttctcccgctcattgtaggtagggataataactatactgtctgtcatcgtgtactttctccgagttcgtttgctgaacatctaatcggcatagccggcactcctttgctacaaagatacgaactttgatatacgcaaacaccttattccaaagcttttgcacatggtcctcattctcgaatgaggcctttcg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0920:PG0922|PG0920:PG0922:glycosyl transferase, group 2 family protein:hypothetical protein:<-<-:981116..981317 202 tttggccacgttctcccgctcattgtaggtagggataataactatactgtctgtcatcgtgtactttctccgagttcgtttgctgaacatctaatcggcatagccggcactcctttgctacaaagatacgaactttgatatacgcaaacaccttattccaaagcttttgcacatggtcctcattctcgaatgaggcctttcg Intra-Species Hit: Count: 1 Min: 1 Max: 202 Len: 202 Subject: NC_002950_PG0920_PG0922|glycosyl transferase, group 2 family protein:hypothetical protein|NEGATIVE:NEGATIVE|[981116,981317]|202 HSP 1 e-value: 1.0E-111 bit: 400.0 Len: 202 Query Start:1 Query End:202 Subject Strand: POSITIVE Subject Start: 1 Subject End: 202 tttggccacgttctcccgctcattgtaggtagggataataactatactgtctgtcatcgtgtactttctccgagttcgtttgctgaacatctaatcggcatagccggcactcctttgctacaaagatacgaactttgatatacgcaaacaccttattccaaagcttttgcacatggtcctcattctcgaatgaggcctttcg tttggccacgttctcccgctcattgtaggtagggataataactatactgtctgtcatcgtgtactttctccgagttcgtttgctgaacatctaatcggcatagccggcactcctttgctacaaagatacgaactttgatatacgcaaacaccttattccaaagcttttgcacatggtcctcattctcgaatgaggcctttcg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUGGCCACGUUCUCCCGCUCAUUGUAGGUAGGGAUAAUAACUAUACUGUCUGUCAUCGUGUACUUUCUCCGAGUUCGUUUGCUGAACAUCUAAUCGGCAUAGCCGGCACUCCUUUGCUACAAAGAUACGAACUUUGAUAUACGCAAACACCUUAUUCCAAAGCUUUUGCACAUGGUCCUCAUUCUCGAAUGAGGCCUUUCG ...((((.(((((.((..(.....)..))..(((((((..((......)).(((...((((((.....((.((((((((.((((((........)))))).....((((......))))........)))))))).))))))))...)))..)))))))...((....)).................))))).))))..... (-47.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGAAAGGCCUCAUUCGAGAAUGAGGACCAUGUGCAAAAGCUUUGGAAUAAGGUGUUUGCGUAUAUCAAAGUUCGUAUCUUUGUAGCAAAGGAGUGCCGGCUAUGCCGAUUAGAUGUUCAGCAAACGAACUCGGAGAAAGUACACGAUGACAGACAGUAUAGUUAUUAUCCCUACCUACAAUGAGCGGGAGAACGUGGCCAAA (....).(((((((....))))))).((((((......((((.......(((((.....((((.((..(((((((......(((((...((....)).)))))((.((........)).))..)))))))..))....))))..(((((..(((......))).)))))..))))).....))))......))))))..... (-50.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 996 120 71 211 162 NC_002950:PG0920|5end_glycosyl_transferase,_group_2_family_protein_981025..981255_NEGATIVE 372 100 54 135 88 NC_002950:PG2186|5end_transcriptional_regulator,_putative_2293619..2293849_NEGATIVE 359 108 66 145 93 NC_002950:PG0423|5end_hypothetical_protein_462251..462481_POSITIVE 356 118 71 160 93 NC_002950:PG1852|5end_exonuclease_1951973..1952203_NEGATIVE 338 107 64 211 169 NC_002950:PG0450|5end_hypothetical_protein_490812..491042_POSITIVE 332 108 71 103 61 NC_002950:PG2010|5end_phosphomannomutase,_putative_2099220..2099450_POSITIVE 327 119 72 180 148 NC_002950:PG0867|5end_hypothetical_protein_930933..931163_NEGATIVE 324 116 72 197 158 NC_002950:PG1945|5end_hypothetical_protein_2031697..2031927_POSITIVE 318 109 64 155 115 NC_002950:PG2120|5end_metallo-beta-lactamase_superfamily_protein_2228492..2228722_NEGATIVE 318 100 55 98 61 NC_002950:PG1837|5end_hemagglutinin_protein_HagA_1930337..1930567_POSITIVE 318 117 71 207 159 NC_002950:PG1163|5end_cobyrinic_acid_a,c-diamide_synthase_1243381..1243611_NEGATIVE 317 110 66 51 18 NC_002950:PG0282|5end_ABC_transporter,_ATP-binding_protein_316915..317145_NEGATIVE 315 118 74 161 107 NC_002950:PG1967|5end_TPR_domain_protein_2057168..2057398_POSITIVE 315 105 64 212 175 NC_002950:PG0415|5end_peptidyl-prolyl_cis-trans_isomerase,_PPIC-type_455096..455326_NEGATIVE 314 37 2 66 36 NC_002950:PG2225|5end_hypothetical_protein_2339440..2339670_POSITIVE 312 200 158 65 29 NC_002950:PG2152|5end_DNA-binding_protein,_histone-like_family_2259750..2259980_POSITIVE 312 200 158 65 29 NC_002950:PG0853|5end_DNA-binding_protein,_histone-like_family_914384..914614_POSITIVE 311 130 88 49 17 NC_002950:PG1596|5end_isoleucyl-tRNA_synthetase,_putative_1674543..1674773_POSITIVE 311 119 80 228 190 NC_002950:PG1156|5end_S4_domain_protein_1236735..1236965_POSITIVE 309 91 51 41 1 NC_002950:PG1217|5end_hypothetical_protein_1295575..1295805_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 358 119 71 62 19 NC_002950:PG2207|3end_hypothetical_protein_2319142..2319292_NEGATIVE 356 118 71 147 80 NC_002950:PG1853|3end_DNA_polymerase_III,_beta_subunit_1951986..1952136_NEGATIVE 349 118 71 117 62 NC_002950:PG0799|3end_hypothetical_protein_854599..854749_POSITIVE 335 36 9 131 102 NC_002950:PG0816|3end_hypothetical_protein_876386..876536_NEGATIVE 330 110 62 147 92 NC_002950:PG0173|3end_transcriptional_regulator,_putative_194388..194538_NEGATIVE 327 117 82 66 32 NC_002950:PG1786|3end_hypothetical_protein_1875322..1875472_POSITIVE 321 109 61 128 80 NC_002950:PG2208|3end_hypothetical_protein_2320332..2320482_NEGATIVE 319 107 64 60 27 NC_002950:PG1391|3end_hypothetical_protein_1472961..1473111_POSITIVE 318 109 64 102 62 NC_002950:PG2121|3end_L-asparaginase_2228545..2228695_NEGATIVE 317 84 41 61 7 NC_002950:PG1247|3end_3-dehydroquinate_synthase_1325585..1325735_POSITIVE 315 100 51 77 32 NC_002950:PG0563|3end_hypothetical_protein_620935..621085_NEGATIVE 314 37 2 46 16 NC_002950:PG2224|3end_hypothetical_protein_2339500..2339650_POSITIVE 309 91 51 51 11 NC_002950:PG1216|3end_hypothetical_protein_1295665..1295815_POSITIVE 307 119 71 62 8 NC_002950:PG1366|3end_UDP-N-acetylglucosamine_1-carboxyvinyltransferase_1446076..1446226_NEGATIVE 306 119 71 137 72 NC_002950:PG1942|3end_30S_ribosomal_protein_S12_2027946..2028096_NEGATIVE 306 177 132 54 9 NC_002950:PG1805|3end_V-type_ATP_synthase_subunit_D_1900283..1900433_POSITIVE 304 98 51 142 88 NC_002950:PG0737|3end_hypothetical_protein_786546..786696_POSITIVE 302 109 62 134 92 NC_002950:PG0347|3end_UDP-glucose_4-epimerase_382382..382532_POSITIVE 300 199 156 119 69 NC_002950:PG2085|3end_tryptophanyl-tRNA_synthetase_2186595..2186745_POSITIVE 297 131 93 69 28 NC_002950:PG1723|3end_ribosomal_protein_S20_1813403..1813553_POSITIVE