Origin IGS: atccttttccgtttggttgattaataagagttgtcgaattctgaatgaaaaatttcgtttagagaggtcaaattaattttcagatcagcggttgtatccgaagcgaatcgggtaatagttttattaccaccaatgggaaatgatgccggataaggcgaaccctacaacttaatttgtccataagcttgccaagcttgtttcctctgatgcaaatttg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 caaatttgcatcagaggaaacaagcttggcaagcttatggacaaattaagttgtagggttcgccttatccggcatcatttcccattggtggtaataaaactattacccgattcgcttcggatacaaccgctgatctgaaaattaatttgacctctctaaacgaaatttttcattcagaattcgacaactcttattaatcaaccaaacggaaaaggat Mask Tandem Repeat Region ================================================ atccttttccgtttggttgattaataagagttgtcgaattctgaatgaaaaatttcgtttagagaggtcaaattaattttcagatcagcggttgtatccgaagcgaatcgggtaatagttttattaccaccaatgggaaatgatgccggataaggcgaaccctacaacttaatttgtccataagcttgccaagcttgtttcctctgatgcaaatttg Find is-nt database================================================ Query_seq: PG0914:PG0915|PG0914:PG0915:hypothetical protein:hypothetical protein:<-->:976508..976724 217 atccttttccgtttggttgattaataagagttgtcgaattctgaatgaaaaatttcgtttagagaggtcaaattaattttcagatcagcggttgtatccgaagcgaatcgggtaatagttttattaccaccaatgggaaatgatgccggataaggcgaaccctacaacttaatttgtccataagcttgccaagcttgtttcctctgatgcaaatttg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0914:PG0915|PG0914:PG0915:hypothetical protein:hypothetical protein:<-->:976508..976724 217 atccttttccgtttggttgattaataagagttgtcgaattctgaatgaaaaatttcgtttagagaggtcaaattaattttcagatcagcggttgtatccgaagcgaatcgggtaatagttttattaccaccaatgggaaatgatgccggataaggcgaaccctacaacttaatttgtccataagcttgccaagcttgtttcctctgatgcaaatttg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0914:PG0915|PG0914:PG0915:hypothetical protein:hypothetical protein:<-->:976508..976724 217 atccttttccgtttggttgattaataagagttgtcgaattctgaatgaaaaatttcgtttagagaggtcaaattaattttcagatcagcggttgtatccgaagcgaatcgggtaatagttttattaccaccaatgggaaatgatgccggataaggcgaaccctacaacttaatttgtccataagcttgccaagcttgtttcctctgatgcaaatttg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atccttttccgtttggttgattaataagagttgtcgaattctgaatgaaaaatttcgtttagagaggtcaaattaattttcagatcagcggttgtatccgaagcgaatcgggtaatagttttattaccaccaatgggaaatgatgccggataaggcgaaccctacaacttaatttgtccataagcttgccaagcttgtttcctctgatgcaaatttg Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 52 End: 150 ................................................... M S F R E V K L I F R S A V V S E A N R V I V L L P P M G N D A G ................................................................... Protein_Len: 44 Strand: - Start: 83 End: 214 .................................................................................. I L P Q I R L S D P L L K I V V L P F H H R I L R V R C S L K D M L K G L K N G R I C M ... Protein_Len: 37 Strand: - Start: 24 End: 134 ....................... Y S N D F E S H F I E N L S T L N I K L D A T T D S A F R T I T K N G G M ................................................................................... atccttttccgtttggttgattaataagagttgtcgaattctgaatgaaaaatttcgtttagagaggtcaaattaattttcagatcagcggttgtatccgaagcgaatcgggtaatagttttattaccaccaatgggaaatgatgccggataaggcgaaccctacaacttaatttgtccataagcttgccaagcttgtttcctctgatgcaaatttg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atccttttccgtttggttgattaataagagttgtcgaattctgaatgaaaaatttcgtttagagaggtcaaattaattttcagatcagcggttgtatccgaagcgaatcgggtaatagttttattaccaccaatgggaaatgatgccggataaggcgaaccctacaacttaatttgtccataagcttgccaagcttgtttcctctgatgcaaatttg Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 47 HP_score: -4.3 Tail_Score: -2.54841 Start: 95 End: 114 Full_Region: TCAGATCAGCGGTTG TATCCGA AGCGAA TCGGGTA ATAGTTTTATTACCA ..............................................................................................TATCCGAAGCGAATCGGGTA....................................................................................................... Find igs database================================================ Query_seq: PG0914:PG0915|PG0914:PG0915:hypothetical protein:hypothetical protein:<-->:976508..976724 217 atccttttccgtttggttgattaataagagttgtcgaattctgaatgaaaaatttcgtttagagaggtcaaattaattttcagatcagcggttgtatccgaagcgaatcgggtaatagttttattaccaccaatgggaaatgatgccggataaggcgaaccctacaacttaatttgtccataagcttgccaagcttgtttcctctgatgcaaatttg Intra-Species Hit: Count: 1 Min: 1 Max: 217 Len: 217 Subject: NC_002950_PG0914_PG0915|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[976508,976724]|217 HSP 1 e-value: 1.0E-120 bit: 430.0 Len: 217 Query Start:1 Query End:217 Subject Strand: POSITIVE Subject Start: 1 Subject End: 217 atccttttccgtttggttgattaataagagttgtcgaattctgaatgaaaaatttcgtttagagaggtcaaattaattttcagatcagcggttgtatccgaagcgaatcgggtaatagttttattaccaccaatgggaaatgatgccggataaggcgaaccctacaacttaatttgtccataagcttgccaagcttgtttcctctgatgcaaatttg atccttttccgtttggttgattaataagagttgtcgaattctgaatgaaaaatttcgtttagagaggtcaaattaattttcagatcagcggttgtatccgaagcgaatcgggtaatagttttattaccaccaatgggaaatgatgccggataaggcgaaccctacaacttaatttgtccataagcttgccaagcttgtttcctctgatgcaaatttg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCCUUUUCCGUUUGGUUGAUUAAUAAGAGUUGUCGAAUUCUGAAUGAAAAAUUUCGUUUAGAGAGGUCAAAUUAAUUUUCAGAUCAGCGGUUGUAUCCGAAGCGAAUCGGGUAAUAGUUUUAUUACCACCAAUGGGAAAUGAUGCCGGAUAAGGCGAACCCUACAACUUAAUUUGUCCAUAAGCUUGCCAAGCUUGUUUCCUCUGAUGCAAAUUUG ........(((((.((((((..(.(((...(((((...(((((((((((....))))))))))).)).))).))).)..))).))))))))(((((((.((.((((((..(((..(((..(((((.(((....)))..))))).(((......))).....)))..)))..))))))..(((((((.....)))))))....)).)))))))..... (-49.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAAAUUUGCAUCAGAGGAAACAAGCUUGGCAAGCUUAUGGACAAAUUAAGUUGUAGGGUUCGCCUUAUCCGGCAUCAUUUCCCAUUGGUGGUAAUAAAACUAUUACCCGAUUCGCUUCGGAUACAACCGCUGAUCUGAAAAUUAAUUUGACCUCUCUAAACGAAAUUUUUCAUUCAGAAUUCGACAACUCUUAUUAAUCAACCAAACGGAAAAGGAU ............(((((....(((((.....))))).....(((((((.(((((((((....)))))..))))....((((..((..(((((((((....))))..((((......)))).....)))))..))..))))..))))))).))))).....((((.((((......))))))))..................((....))........ (-39.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 915 130 81 221 172 NC_002950:PG0914|5end_hypothetical_protein_976417..976647_NEGATIVE 365 100 51 151 99 NC_002950:PG0352|5end_sialidase,_putative_387187..387417_POSITIVE 328 180 135 230 176 NC_002950:PG2047|5end_helicase,_putative_2148043..2148273_NEGATIVE 320 118 80 83 37 NC_002950:PG0480|5end_precorrin-2_C20-methyltransferase,_putative_521319..521549_NEGATIVE 309 114 76 207 171 NC_002950:PG2027|5end_hypothetical_protein_2125494..2125724_POSITIVE 300 210 174 197 147 NC_002950:PG0293|5end_secretion_activator_protein,_putative_328671..328901_NEGATIVE 292 170 127 176 131 NC_002950:PG1947|5end_TPR_domain_protein_2032932..2033162_POSITIVE 290 189 142 43 2 NC_002950:PG0381|5end_cell_volume_regulation_protein_CvrA_412705..412935_NEGATIVE 290 121 85 67 33 NC_002950:PG0022|5end_sulfate_permease_family_protein_26796..27026_NEGATIVE 289 120 81 161 119 NC_002950:PG1479|5end_conjugative_transposon_protein_TraJ_1555234..1555464_NEGATIVE 287 114 80 187 143 NC_002950:PG1180|5end_hypothetical_protein_1263615..1263845_NEGATIVE 286 112 81 76 50 NC_002950:PG1582|5end_batA_protein_1659321..1659551_POSITIVE 283 112 75 44 4 NC_002950:PG0702|5end_nicotinate-nucleotide--dimethylbenzimidazole_phosphoribosyltransferase,_putative_757100..757330_POSITIVE 283 170 126 188 148 NC_002950:PG0387|5end_elongation_factor_Tu_420643..420873_POSITIVE 282 129 82 101 39 NC_002950:PG1333|5end_hypothetical_protein_1415425..1415655_NEGATIVE 281 189 143 138 90 NC_002950:PG1383|5end_amino_acid_exporter,_putative_1462669..1462899_POSITIVE 280 190 144 225 179 NC_002950:PG1057|5end_hypothetical_protein_1123419..1123649_POSITIVE 278 128 87 52 13 NC_002950:PG1561|5end_dTDP-4-dehydrorhamnose_reductase_1640995..1641225_NEGATIVE 274 190 144 206 157 NC_002950:PG1068|5end_hypothetical_protein_1134636..1134866_POSITIVE 274 129 83 135 81 NC_002950:PG0975|5end_PhoH_family_protein_1037284..1037514_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 362 190 146 145 96 NC_002950:PG0800|3end_hypothetical_protein_854719..854869_NEGATIVE 328 180 135 139 85 NC_002950:PG2048|3end_hypothetical_protein_2148134..2148284_NEGATIVE 295 118 81 71 33 NC_002950:PG1509|3end_HAD-superfamily_hydrolase,_subfamily_IA,_variant_1_family_protein_1587439..1587589_POSITIVE 293 115 83 149 118 NC_002950:PG1303|3end_helicase,_putative_1382147..1382297_POSITIVE 289 120 81 129 87 NC_002950:PG1480|3end_conjugative_transposon_protein_TraI_1555266..1555416_NEGATIVE 287 114 80 146 102 NC_002950:PG1181|3end_transcriptional_regulator,_tetR_family_1263656..1263806_NEGATIVE 286 112 81 79 53 NC_002950:PG1581|3end_hypothetical_protein_1659404..1659554_POSITIVE 282 129 82 104 42 NC_002950:PG1334|3end_band_7/Mec-2_family_protein_1415422..1415572_NEGATIVE 278 128 82 105 49 NC_002950:PG1943|3end_hypothetical_protein_2028974..2029124_NEGATIVE 278 128 87 55 16 NC_002950:PG1562|3end_dTDP-4-dehydrorhamnose_3,5-epimerase_1640992..1641142_NEGATIVE 275 188 142 83 39 NC_002950:PG0231|3end_hypothetical_protein_267409..267559_POSITIVE 274 110 63 60 16 NC_002950:PG0245|3end_universal_stress_protein_family_279773..279923_NEGATIVE 273 190 141 144 99 NC_002950:PG1470|3end_hypothetical_protein_1548431..1548581_POSITIVE 273 123 82 59 10 NC_002950:PG1417|3end_fumarate_hydratase_class_I,_anaerobic_1503021..1503171_POSITIVE 272 130 87 117 87 NC_002950:PG1012|3end_tRNA-i(6)A37_modification_enzyme_MiaB_1070089..1070239_NEGATIVE 271 118 86 100 64 NC_002950:PG1829|3end_long-chain-fatty-acid--CoA_ligase,_putative_1921307..1921457_NEGATIVE 271 149 101 80 31 NC_002950:PG1782|3end_hypothetical_protein_1871961..1872111_POSITIVE 271 124 82 70 30 NC_002950:PG1278|3end_phosphoserine_aminotransferase_1356514..1356664_POSITIVE 271 149 108 137 96 NC_002950:PG0759|3end_TPR_domain_protein_808632..808782_NEGATIVE 268 162 130 147 118 NC_002950:PG0327|3end_hypothetical_protein_360907..361057_NEGATIVE