Origin IGS: cgttggatcaggataaaatgagataaaacaaaaaacaaccatgatttacgcctacctcaaagcaggagaaaaccatggctgtatgctaagctgcttgggaatgcagacagctatgctgtgggagga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tcctcccacagcatagctgtctgcattcccaagcagcttagcatacagccatggttttctcctgctttgaggtaggcgtaaatcatggttgttttttgttttatctcattttatcctgatccaacg Mask Tandem Repeat Region ================================================ cgttggatcaggataaaatgagataaaacaaaaaacaaccatgatttacgcctacctcaaagcaggagaaaaccatggctgtatgctaagctgcttgggaatgcagacagctatgctgtgggagga Find is-nt database================================================ Query_seq: PG0902:PG0903|PG0902:PG0903:alpha-1,2-mannosidase family protein:hypothetical protein:<-<-:969062..969187 126 cgttggatcaggataaaatgagataaaacaaaaaacaaccatgatttacgcctacctcaaagcaggagaaaaccatggctgtatgctaagctgcttgggaatgcagacagctatgctgtgggagga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0902:PG0903|PG0902:PG0903:alpha-1,2-mannosidase family protein:hypothetical protein:<-<-:969062..969187 126 cgttggatcaggataaaatgagataaaacaaaaaacaaccatgatttacgcctacctcaaagcaggagaaaaccatggctgtatgctaagctgcttgggaatgcagacagctatgctgtgggagga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0902:PG0903|PG0902:PG0903:alpha-1,2-mannosidase family protein:hypothetical protein:<-<-:969062..969187 126 cgttggatcaggataaaatgagataaaacaaaaaacaaccatgatttacgcctacctcaaagcaggagaaaaccatggctgtatgctaagctgcttgggaatgcagacagctatgctgtgggagga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cgttggatcaggataaaatgagataaaacaaaaaacaaccatgatttacgcctacctcaaagcaggagaaaaccatggctgtatgctaagctgcttgggaatgcagacagctatgctgtgggagga Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 23 End: 124 ...................... M K Q K T T M I Y A Y L K A G E N H G C M L S C L G M Q T A M L W E .. cgttggatcaggataaaatgagataaaacaaaaaacaaccatgatttacgcctacctcaaagcaggagaaaaccatggctgtatgctaagctgcttgggaatgcagacagctatgctgtgggagga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cgttggatcaggataaaatgagataaaacaaaaaacaaccatgatttacgcctacctcaaagcaggagaaaaccatggctgtatgctaagctgcttgggaatgcagacagctatgctgtgggagga Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -10.5 Tail_Score: -5.8901 Start: 36 End: 81 Full_Region: aagcagcttagcata cagccatggttttctcctgct ttga ggtaggcgtaaatcatggttg ttttttgttttatct ...................................cagccatggttttctcctgctttgaggtaggcgtaaatcatggttg............................................. Find igs database================================================ Query_seq: PG0902:PG0903|PG0902:PG0903:alpha-1,2-mannosidase family protein:hypothetical protein:<-<-:969062..969187 126 cgttggatcaggataaaatgagataaaacaaaaaacaaccatgatttacgcctacctcaaagcaggagaaaaccatggctgtatgctaagctgcttgggaatgcagacagctatgctgtgggagga Intra-Species Hit: Count: 1 Min: 1 Max: 126 Len: 126 Subject: NC_002950_PG0902_PG0903|alpha-1,2-mannosidase family protein:hypothetical protein|NEGATIVE:NEGATIVE|[969062,969187]|126 HSP 1 e-value: 9.0E-66 bit: 250.0 Len: 126 Query Start:1 Query End:126 Subject Strand: POSITIVE Subject Start: 1 Subject End: 126 cgttggatcaggataaaatgagataaaacaaaaaacaaccatgatttacgcctacctcaaagcaggagaaaaccatggctgtatgctaagctgcttgggaatgcagacagctatgctgtgggagga cgttggatcaggataaaatgagataaaacaaaaaacaaccatgatttacgcctacctcaaagcaggagaaaaccatggctgtatgctaagctgcttgggaatgcagacagctatgctgtgggagga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGUUGGAUCAGGAUAAAAUGAGAUAAAACAAAAAACAACCAUGAUUUACGCCUACCUCAAAGCAGGAGAAAACCAUGGCUGUAUGCUAAGCUGCUUGGGAAUGCAGACAGCUAUGCUGUGGGAGGA (((.((((((((..........................)).)))))))))....((((...((((........(((((((((.(((....((.....))...))).)))))))))))))..)))). (-31.17) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCCUCCCACAGCAUAGCUGUCUGCAUUCCCAAGCAGCUUAGCAUACAGCCAUGGUUUUCUCCUGCUUUGAGGUAGGCGUAAAUCAUGGUUGUUUUUUGUUUUAUCUCAUUUUAUCCUGAUCCAACG ..........((..(((((..((......))..)))))..))..(((((((((((((.(.((((((....)))))).).))))))))))))).....(((..(((............)))..))). (-37.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1010 120 71 211 162 NC_002950:PG0902|5end_alpha-1,2-mannosidase_family_protein_968971..969201_NEGATIVE 436 87 41 48 5 NC_002950:PG1770|5end_hypothetical_protein_1857284..1857514_POSITIVE 372 99 51 145 104 NC_002950:PG2159|5end_protoporphyrinogen_oxidase_2264397..2264627_POSITIVE 344 108 61 171 113 NC_002950:PG0018|5end_hypothetical_protein_19648..19878_POSITIVE 342 120 76 223 183 NC_002950:PG0634|5end_ThiJ/PfpI_family_protein_686143..686373_POSITIVE 339 120 74 200 149 NC_002950:PG1169|5end_hypothetical_protein_1249181..1249411_NEGATIVE 337 88 49 186 140 NC_002950:PG2021|5end_hypothetical_protein_2114852..2115082_NEGATIVE 336 117 76 197 150 NC_002950:PG1253|5end_DNA_ligase,_NAD-dependent_1331606..1331836_NEGATIVE 335 120 77 49 1 NC_002950:PG0161|5end_hypothetical_protein_184416..184646_NEGATIVE 326 109 68 181 142 NC_002950:PG1811|5end_hypothetical_protein_1906803..1907033_POSITIVE 320 109 61 185 144 NC_002950:PG0597|5end_ribosomal_protein_L9_653662..653892_POSITIVE 318 119 74 81 39 NC_002950:PG0486|5end_methylated-DNA--protein-cysteine_S-methyltransferase_525934..526164_NEGATIVE 313 118 73 68 23 NC_002950:PG1480|5end_conjugative_transposon_protein_TraI_1555895..1556125_NEGATIVE 309 117 73 123 70 NC_002950:PG0458|5end_ISPg5,_transposase_Orf2_501463..501693_NEGATIVE 307 120 72 194 150 NC_002950:PG1805|5end_V-type_ATP_synthase_subunit_D_1899589..1899819_POSITIVE 307 118 73 165 117 NC_002950:PG0201|5end_ribonuclease_P_238770..239000_NEGATIVE 305 118 73 127 88 NC_002950:PG1028|5end_TPR_domain_protein_1089670..1089900_POSITIVE 305 81 41 152 112 NC_002950:PG0508|5end_HAD-superfamily_subfamily_IB_hydrolase,_TIGR01490_548738..548968_NEGATIVE 305 98 52 51 3 NC_002950:PG0223|5end_exonuclease_257610..257840_NEGATIVE 304 116 79 66 20 NC_002950:PG2029|5end_hypothetical_protein_2126543..2126773_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 372 99 51 141 100 NC_002950:PG2158|3end_hypothetical_protein_2264473..2264623_POSITIVE 352 120 71 118 75 NC_002950:PG0085|3end_alpha-galactosidase_100556..100706_POSITIVE 351 116 72 100 37 NC_002950:PG1152|3end_hypothetical_protein_1234674..1234824_NEGATIVE 351 120 74 151 103 NC_002950:PG0167|3end_ribosomal_protein_L25_188512..188662_NEGATIVE 344 107 64 42 1 NC_002950:PG0509|3end_hypothetical_protein_548728..548878_NEGATIVE 316 99 56 101 63 NC_002950:PG2227|3end_hypothetical_protein_2342744..2342894_POSITIVE 314 114 74 86 42 NC_002950:PG0378|3end_elongation_factor_Ts_408808..408958_POSITIVE 309 117 73 69 16 NC_002950:PG0459|3end_ISPg5,_transposase_Orf1_501517..501667_NEGATIVE 304 116 79 59 13 NC_002950:PG2028|3end_ebsC_protein_2126616..2126766_POSITIVE 299 118 77 41 2 NC_002950:PG1481|3end_conjugative_transposon_protein_TraG_1555922..1556072_NEGATIVE 296 104 61 140 101 NC_002950:PG0214|3end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_251543..251693_POSITIVE 295 100 57 145 96 NC_002950:PG0356|3end_hypothetical_protein_391473..391623_POSITIVE 291 103 62 144 106 NC_002950:PG0259|3end_hypothetical_protein_293701..293851_POSITIVE 291 94 57 148 104 NC_002950:PG0017|3end_sensor_histidine_kinase_19724..19874_POSITIVE 286 87 49 59 9 NC_002950:PG1587|3end_PAP2_superfamily_protein_1665779..1665929_POSITIVE 283 98 60 100 61 NC_002950:PG1841|3end_hypothetical_protein_1938313..1938463_POSITIVE 283 119 73 97 52 NC_002950:PG1759|3end_adhesion_protein,_putative_1846597..1846747_POSITIVE 283 118 76 147 92 NC_002950:PG0144|3end_hypothetical_protein_166643..166793_NEGATIVE 282 109 61 141 74 NC_002950:PG1110|3end_hypothetical_protein_1183237..1183387_POSITIVE 282 119 74 126 84 NC_002950:PG1078|3end_electron_transfer_flavoprotein,_alpha_subunit_1146525..1146675_POSITIVE