Origin IGS: agttttttcgattcaaaaatgaaaaaccgtgcaggtctcgatttttttgctgtggtgattcagctttcttacaaatataggatgggaattgtttttggggacgcgtttcctctctaaaatatcacattcggctcaatggagtcgttgttttgtttgtttttgaaagatcatcaagtaccaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttggtacttgatgatctttcaaaaacaaacaaaacaacgactccattgagccgaatgtgatattttagagaggaaacgcgtccccaaaaacaattcccatcctatatttgtaagaaagctgaatcaccacagcaaaaaaatcgagacctgcacggtttttcatttttgaatcgaaaaaact Mask Tandem Repeat Region ================================================ agttttttcgattcaaaaatgaaaaaccgtgcaggtctcgatttttttgctgtggtgattcagctttcttacaaatataggatgggaattgtttttggggacgcgtttcctctctaaaatatcacattcggctcaatggagtcgttgttttgtttgtttttgaaagatcatcaagtaccaaa Find is-nt database================================================ Query_seq: PG0901:PG0902|PG0901:PG0902:hypothetical protein:alpha-1,2-mannosidase family protein:<-<-:966654..966835 182 agttttttcgattcaaaaatgaaaaaccgtgcaggtctcgatttttttgctgtggtgattcagctttcttacaaatataggatgggaattgtttttggggacgcgtttcctctctaaaatatcacattcggctcaatggagtcgttgttttgtttgtttttgaaagatcatcaagtaccaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0901:PG0902|PG0901:PG0902:hypothetical protein:alpha-1,2-mannosidase family protein:<-<-:966654..966835 182 agttttttcgattcaaaaatgaaaaaccgtgcaggtctcgatttttttgctgtggtgattcagctttcttacaaatataggatgggaattgtttttggggacgcgtttcctctctaaaatatcacattcggctcaatggagtcgttgttttgtttgtttttgaaagatcatcaagtaccaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0901:PG0902|PG0901:PG0902:hypothetical protein:alpha-1,2-mannosidase family protein:<-<-:966654..966835 182 agttttttcgattcaaaaatgaaaaaccgtgcaggtctcgatttttttgctgtggtgattcagctttcttacaaatataggatgggaattgtttttggggacgcgtttcctctctaaaatatcacattcggctcaatggagtcgttgttttgtttgtttttgaaagatcatcaagtaccaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agttttttcgattcaaaaatgaaaaaccgtgcaggtctcgatttttttgctgtggtgattcagctttcttacaaatataggatgggaattgtttttggggacgcgtttcctctctaaaatatcacattcggctcaatggagtcgttgttttgtttgtttttgaaagatcatcaagtaccaaa Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 19 End: 114 .................. M K N R A G L D F F A V V I Q L S Y K Y R M G I V F G D A F P L .................................................................... Protein_Len: 35 Strand: - Start: 16 End: 120 ............... F H F V T C T E I K K S H H N L K R V F I P H S N N K P V R K R E L M .............................................................. agttttttcgattcaaaaatgaaaaaccgtgcaggtctcgatttttttgctgtggtgattcagctttcttacaaatataggatgggaattgtttttggggacgcgtttcctctctaaaatatcacattcggctcaatggagtcgttgttttgtttgtttttgaaagatcatcaagtaccaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agttttttcgattcaaaaatgaaaaaccgtgcaggtctcgatttttttgctgtggtgattcagctttcttacaaatataggatgggaattgtttttggggacgcgtttcctctctaaaatatcacattcggctcaatggagtcgttgttttgtttgtttttgaaagatcatcaagtaccaaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 77 HP_score: -6.8 Tail_Score: -4.64595 Start: 129 End: 144 Full_Region: CTAAAATATCACATT CGGCTC AATG GAGTCG TTGTTTTGTTTGTTT ................................................................................................................................CGGCTCAATGGAGTCG...................................... TransTerm Strand: + Conf: 75 HP_score: -3.1 Tail_Score: -5.3167 Start: 134 End: 147 Full_Region: ATATCACATTCGGCT CAATG GAGT CGTTG TTTTGTTTGTTTTTG .....................................................................................................................................CAATGGAGTCGTTG................................... Find igs database================================================ Query_seq: PG0901:PG0902|PG0901:PG0902:hypothetical protein:alpha-1,2-mannosidase family protein:<-<-:966654..966835 182 agttttttcgattcaaaaatgaaaaaccgtgcaggtctcgatttttttgctgtggtgattcagctttcttacaaatataggatgggaattgtttttggggacgcgtttcctctctaaaatatcacattcggctcaatggagtcgttgttttgtttgtttttgaaagatcatcaagtaccaaa Intra-Species Hit: Count: 1 Min: 1 Max: 182 Len: 182 Subject: NC_002950_PG0901_PG0902|hypothetical protein:alpha-1,2-mannosidase family protein|NEGATIVE:NEGATIVE|[966654,966835]|182 HSP 1 e-value: 2.0E-86 bit: 319.0 Len: 182 Query Start:1 Query End:182 Subject Strand: POSITIVE Subject Start: 1 Subject End: 182 agttttttcgattcaaaaatgaaaaaccgtgcaggtctcgannnnnnngctgtggtgattcagctttcttacaaatataggatgggaattgtttttggggacgcgtttcctctctaaaatatcacattcggctcaatggagtcgttgttttgtttgtttttgaaagatcatcaagtaccaaa agttttttcgattcaaaaatgaaaaaccgtgcaggtctcgatttttttgctgtggtgattcagctttcttacaaatataggatgggaattgtttttggggacgcgtttcctctctaaaatatcacattcggctcaatggagtcgttgttttgtttgtttttgaaagatcatcaagtaccaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUUUUUUCGAUUCAAAAAUGAAAAACCGUGCAGGUCUCGAUUUUUUUGCUGUGGUGAUUCAGCUUUCUUACAAAUAUAGGAUGGGAAUUGUUUUUGGGGACGCGUUUCCUCUCUAAAAUAUCACAUUCGGCUCAAUGGAGUCGUUGUUUUGUUUGUUUUUGAAAGAUCAUCAAGUACCAAA .......((((..(....(((......)))....)..))))......((((.(((((((....(((((..(((((((..(((.(((((.(((....(....)))).))))).)))........(((..((((((....)))))).)))..)))))))....))))))))))))))))..... (-41.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUGGUACUUGAUGAUCUUUCAAAAACAAACAAAACAACGACUCCAUUGAGCCGAAUGUGAUAUUUUAGAGAGGAAACGCGUCCCCAAAAACAAUUCCCAUCCUAUAUUUGUAAGAAAGCUGAAUCACCACAGCAAAAAAAUCGAGACCUGCACGGUUUUUCAUUUUUGAAUCGAAAAAACU ...(((.((((((..((((.((((..............((.(((....))).))..(((...........(.(....).)..........)))..............)))).))))..((((........))))......)))))))))....((((((.........))))))........ (-28.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 912 140 91 231 182 NC_002950:PG0901|5end_hypothetical_protein_966563..966793_NEGATIVE 359 106 62 206 156 NC_002950:PG2067|5end_pyridoxal_phosphate_biosynthetic_protein_PdxA_2163215..2163445_POSITIVE 334 70 30 99 46 NC_002950:PG0645|5end_hypothetical_protein_697959..698189_NEGATIVE 331 148 102 73 20 NC_002950:PG1987|5end_CRISPR-associated_TM1811_family_protein_2077432..2077662_NEGATIVE 318 63 27 64 16 NC_002950:PG2060|5end_thymidylate_synthase_2157573..2157803_POSITIVE 317 146 101 183 118 NC_002950:PG1009|5end_hypothetical_protein_1068495..1068725_NEGATIVE 314 145 107 87 40 NC_002950:PG2139|5end_hypothetical_protein_2245648..2245878_POSITIVE 312 64 28 76 40 NC_002950:PG2202|5end_Holliday_junction_resolvase-like_protein_2309933..2310163_NEGATIVE 309 68 28 157 117 NC_002950:PG0164|5end_hypothetical_protein_187395..187625_NEGATIVE 308 64 23 214 167 NC_002950:PG0080|5end_hypothetical_protein_93832..94062_NEGATIVE 301 70 28 46 1 NC_002950:PG0167|5end_ribosomal_protein_L25_189090..189320_NEGATIVE 298 69 28 69 33 NC_002950:PG0068|5end_hypothetical_protein_81748..81978_POSITIVE 292 64 28 87 46 NC_002950:PG2162|5end_lipid_A_disaccharide_synthase_2268764..2268994_NEGATIVE 292 96 51 86 43 NC_002950:PG1253|5end_DNA_ligase,_NAD-dependent_1331606..1331836_NEGATIVE 291 109 80 208 182 NC_002950:PG0349|5end_hydrolase,_haloacid_dehalogenase-like_family_384459..384689_POSITIVE 288 149 101 126 59 NC_002950:PG0488|5end_Holliday_junction_DNA_helicase_RuvB_528169..528399_POSITIVE 287 84 49 227 188 NC_002950:PG1709|5end_ISPg5,_transposase_Orf1_1794914..1795144_POSITIVE 286 110 62 62 11 NC_002950:PG1232|5end_glutamate_dehydrogenase_1309611..1309841_NEGATIVE 285 150 106 77 31 NC_002950:PG1100|5end_hypothetical_protein_1169012..1169242_NEGATIVE 284 139 93 93 49 NC_002950:PG1351|5end_hypothetical_protein_1432744..1432974_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 331 148 102 80 27 NC_002950:PG1988|3end_hypothetical_protein_2077425..2077575_NEGATIVE 323 113 80 139 99 NC_002950:PG1747|3end_ribose_5-phosphate_isomerase_B,_putative_1832378..1832528_NEGATIVE 320 64 26 142 95 NC_002950:PG1296|3end_hypothetical_protein_1375329..1375479_POSITIVE 316 66 30 126 84 NC_002950:PG1743|3end_2-dehydro-3-deoxyphosphooctonate_aldolase_1827530..1827680_NEGATIVE 313 115 71 47 6 NC_002950:PG1685|3end_hypothetical_protein_1772216..1772366_NEGATIVE 311 113 73 53 12 NC_002950:PG0932|3end_DNA_polymerase_III,_delta_prime_subunit,_putative_989833..989983_POSITIVE 304 68 27 54 8 NC_002950:PG0507|3end_hypothetical_protein_548190..548340_POSITIVE 302 57 27 66 33 NC_002950:PG1351|3end_hypothetical_protein_1432238..1432388_NEGATIVE 300 115 79 79 49 NC_002950:PG0143|3end_hydrolase,_carbon-nitrogen_family_165747..165897_NEGATIVE 292 64 28 50 9 NC_002950:PG2163|3end_acid_phosphatase_2268801..2268951_NEGATIVE 292 96 51 98 55 NC_002950:PG1254|3end_acetyltransferase,_GNAT_family_1331594..1331744_NEGATIVE 289 64 26 89 47 NC_002950:PG1745|3end_phosphoribulokinase_family_protein_1829435..1829585_NEGATIVE 285 150 106 80 34 NC_002950:PG1101|3end_sodium:solute_symporter_family_protein_1169009..1169159_NEGATIVE 284 139 93 100 56 NC_002950:PG1352|3end_hypothetical_protein_1432737..1432887_NEGATIVE 278 116 79 136 104 NC_002950:PG0706|3end_hypothetical_protein_760882..761032_POSITIVE 277 64 27 103 64 NC_002950:PG2217|3end_1-deoxy-D-xylulose-5-phosphate_synthase_2331607..2331757_POSITIVE 277 100 54 125 82 NC_002950:PG1906|3end_ISPg1,_transposase_2006572..2006722_POSITIVE 277 100 54 125 82 NC_002950:PG1624|3end_ISPg1,_transposase_1706188..1706338_NEGATIVE 277 100 54 125 82 NC_002950:PG1448|3end_ISPg1,_transposase_1533449..1533599_POSITIVE 277 100 54 125 82 NC_002950:PG1197|3end_ISPg1,_transposase_1275475..1275625_NEGATIVE