Origin IGS: atgtgtccgcagctacttcgaatgcgaagagaacgggacattaattctctttcggttgaagccggaacggcattggtttattttccaatcttcttgaagtatagacacaaaagcattgaaaattcttcgaaatactcctaagctttctgcggaatactttgaactattcggtgatgcggtttgaactgttcggggaaatagtttgaactatccggcaaaatagttataactatccggtgaaatagttataactattcgacgaaatagttataactattcagtcaaatactttgatctattccgcgaagagcacagaagtatttgagagaaggcttattcggagccgccttgaaggaaaagttttcagatttggaaaactaaaattcctactgatcatatgctcaaacagcatacgattctctttataagaacgaggatgtttcattcttttcgatacaccctcgtttgttttttgcacccgttgaaagagctataggagaattggcttgatccgt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 acggatcaagccaattctcctatagctctttcaacgggtgcaaaaaacaaacgagggtgtatcgaaaagaatgaaacatcctcgttcttataaagagaatcgtatgctgtttgagcatatgatcagtaggaattttagttttccaaatctgaaaacttttccttcaaggcggctccgaataagccttctctcaaatacttctgtgctcttcgcggaatagatcaaagtatttgactgaatagttataactatttcgtcgaatagttataactatttcaccggatagttataactattttgccggatagttcaaactatttccccgaacagttcaaaccgcatcaccgaatagttcaaagtattccgcagaaagcttaggagtatttcgaagaattttcaatgcttttgtgtctatacttcaagaagattggaaaataaaccaatgccgttccggcttcaaccgaaagagaattaatgtcccgttctcttcgcattcgaagtagctgcggacacat Mask Tandem Repeat Region ================================================ atgtgtccgcagctacttcgaatgcgaagagaacgggacattaattctctttcggttgaagccggaacggcattggtttattttccaatcttcttgaagtatagacacaaaagcattgaaaattcttcgaaatactcctaagctttctgcggaatactttgaactattcggtgatgcggtttgaactgttcgggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntccgcgaagagcacagaagtatttgagagaaggcttattcggagccgccttgaaggaaaagttttcagatttggaaaactaaaattcctactgatcatatgctcaaacagcatacgattctctttataagaacgaggatgtttcattcttttcgatacaccctcgtttgttttttgcacccgttgaaagagctataggagaattggcttgatccgt Find is-nt database================================================ Query_seq: PG0896:PG0897|PG0896:PG0897:beta-galactosidase:alpha-amylase family protein:-><-:960443..960957 515 atgtgtccgcagctacttcgaatgcgaagagaacgggacattaattctctttcggttgaagccggaacggcattggtttattttccaatcttcttgaagtatagacacaaaagcattgaaaattcttcgaaatactcctaagctttctgcggaatactttgaactattcggtgatgcggtttgaactgttcggggaaatagtttgaactatccggcaaaatagttataactatccggtgaaatagttataactattcgacgaaatagttataactattcagtcaaatactttgatctattccgcgaagagcacagaagtatttgagagaaggcttattcggagccgccttgaaggaaaagttttcagatttggaaaactaaaattcctactgatcatatgctcaaacagcatacgattctctttataagaacgaggatgtttcattcttttcgatacaccctcgtttgttttttgcacccgttgaaagagctataggagaattggcttgatccgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0896:PG0897|PG0896:PG0897:beta-galactosidase:alpha-amylase family protein:-><-:960443..960957 515 atgtgtccgcagctacttcgaatgcgaagagaacgggacattaattctctttcggttgaagccggaacggcattggtttattttccaatcttcttgaagtatagacacaaaagcattgaaaattcttcgaaatactcctaagctttctgcggaatactttgaactattcggtgatgcggtttgaactgttcggggaaatagtttgaactatccggcaaaatagttataactatccggtgaaatagttataactattcgacgaaatagttataactattcagtcaaatactttgatctattccgcgaagagcacagaagtatttgagagaaggcttattcggagccgccttgaaggaaaagttttcagatttggaaaactaaaattcctactgatcatatgctcaaacagcatacgattctctttataagaacgaggatgtttcattcttttcgatacaccctcgtttgttttttgcacccgttgaaagagctataggagaattggcttgatccgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0896:PG0897|PG0896:PG0897:beta-galactosidase:alpha-amylase family protein:-><-:960443..960957 515 atgtgtccgcagctacttcgaatgcgaagagaacgggacattaattctctttcggttgaagccggaacggcattggtttattttccaatcttcttgaagtatagacacaaaagcattgaaaattcttcgaaatactcctaagctttctgcggaatactttgaactattcggtgatgcggtttgaactgttcggggaaatagtttgaactatccggcaaaatagttataactatccggtgaaatagttataactattcgacgaaatagttataactattcagtcaaatactttgatctattccgcgaagagcacagaagtatttgagagaaggcttattcggagccgccttgaaggaaaagttttcagatttggaaaactaaaattcctactgatcatatgctcaaacagcatacgattctctttataagaacgaggatgtttcattcttttcgatacaccctcgtttgttttttgcacccgttgaaagagctataggagaattggcttgatccgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atgtgtccgcagctacttcgaatgcgaagagaacgggacattaattctctttcggttgaagccggaacggcattggtttattttccaatcttcttgaagtatagacacaaaagcattgaaaattcttcgaaatactcctaagctttctgcggaatactttgaactattcggtgatgcggtttgaactgttcggggaaatagtttgaactatccggcaaaatagttataactatccggtgaaatagttataactattcgacgaaatagttataactattcagtcaaatactttgatctattccgcgaagagcacagaagtatttgagagaaggcttattcggagccgccttgaaggaaaagttttcagatttggaaaactaaaattcctactgatcatatgctcaaacagcatacgattctctttataagaacgaggatgtttcattcttttcgatacaccctcgtttgttttttgcacccgttgaaagagctataggagaattggcttgatccgt Predict ORF larger than 30AA ================================================ Protein_Len: 46 Strand: + Start: 1 End: 138 M C P Q L L R M R R E R D I N S L S V E A G T A L V Y F P I F L K Y R H K S I E N S S K Y S ......................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 54 Strand: + Start: 264 End: 425 ....................................................................................................................................................................................................................................................................... M V I T I Q S N T L I Y S A K S T E V F E R R L I R S R L E G K V F R F G K L K F L L I I C S N S I R F S L .......................................................................................... Protein_Len: 42 Strand: + Start: 368 End: 493 ............................................................................................................................................................................................................................................................................................................................................................................... M W K T K I P T D H M L K Q H T I L F I R T R M F H S F R Y T L V C F L H P L K E L ...................... Protein_Len: 60 Strand: - Start: 299 End: 478 .......................................................................................................................................................................................................................................................................................................... E A F L V S T N S L L S I R L R R S P F T K L N P F S F N R S I M H E F L M R N E K Y S R P H K M R K S V G E N T K Q M ..................................... Protein_Len: 31 Strand: - Start: 141 End: 233 ............................................................................................................................................ A K Q P I S Q V I R H H P K F Q E P F Y N S S D P L I T I V M .......................................................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: - Start: 44 End: 133 ........................................... N E K P Q L R F P M P K N E L R R S T Y V C F C Q F N K S M .............................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 36 Strand: - Start: 16 End: 123 ............... K S H S S F P V N I R K R N F G S R C Q N I K W D E Q L I S V F A N F M ........................................................................................................................................................................................................................................................................................................................................................................................................ atgtgtccgcagctacttcgaatgcgaagagaacgggacattaattctctttcggttgaagccggaacggcattggtttattttccaatcttcttgaagtatagacacaaaagcattgaaaattcttcgaaatactcctaagctttctgcggaatactttgaactattcggtgatgcggtttgaactgttcggggaaatagtttgaactatccggcaaaatagttataactatccggtgaaatagttataactattcgacgaaatagttataactattcagtcaaatactttgatctattccgcgaagagcacagaagtatttgagagaaggcttattcggagccgccttgaaggaaaagttttcagatttggaaaactaaaattcctactgatcatatgctcaaacagcatacgattctctttataagaacgaggatgtttcattcttttcgatacaccctcgtttgttttttgcacccgttgaaagagctataggagaattggcttgatccgt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 115 End: 144 ..................................................................................................................ATTGAAAATTCTTCGAAATACTCCTAAGCT................................................................................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atgtgtccgcagctacttcgaatgcgaagagaacgggacattaattctctttcggttgaagccggaacggcattggtttattttccaatcttcttgaagtatagacacaaaagcattgaaaattcttcgaaatactcctaagctttctgcggaatactttgaactattcggtgatgcggtttgaactgttcggggaaatagtttgaactatccggcaaaatagttataactatccggtgaaatagttataactattcgacgaaatagttataactattcagtcaaatactttgatctattccgcgaagagcacagaagtatttgagagaaggcttattcggagccgccttgaaggaaaagttttcagatttggaaaactaaaattcctactgatcatatgctcaaacagcatacgattctctttataagaacgaggatgtttcattcttttcgatacaccctcgtttgttttttgcacccgttgaaagagctataggagaattggcttgatccgt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 90 HP_score: -11.6 Tail_Score: -4.10429 Start: 221 End: 276 Full_Region: aagtatttgactgaa tagttataactatttcgtcgaatagt tata actatttcaccggatagttataacta ttttgccggatagtt ............................................................................................................................................................................................................................tagttataactatttcgtcgaatagttataactatttcaccggatagttataacta............................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 58 HP_score: -6.7 Tail_Score: -3.02144 Start: 223 End: 252 Full_Region: CTATCCGGCAAAATA GTTATAACTAT CCGGTGAA ATAGTTATAAC TATTCGACGAAATAG ..............................................................................................................................................................................................................................GTTATAACTATCCGGTGAAATAGTTATAAC....................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 83 HP_score: -11.4 Tail_Score: -3.07291 Start: 243 End: 276 Full_Region: AACTATCCGGTGAAA TAGTTATAACTATT CGACGA AATAGTTATAACTA TTCAGTCAAATACTT ..................................................................................................................................................................................................................................................TAGTTATAACTATTCGACGAAATAGTTATAACTA............................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 90 HP_score: -12.1 Tail_Score: -3.54919 Start: 243 End: 276 Full_Region: aagtatttgactgaa tagttataactattt cgtc gaatagttataacta tttcaccggatagtt ..................................................................................................................................................................................................................................................tagttataactatttcgtcgaatagttataacta............................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 74 HP_score: -9.6 Tail_Score: -3.09327 Start: 245 End: 274 Full_Region: CTATCCGGTGAAATA GTTATAACTATT CGACGA AATAGTTATAAC TATTCAGTCAAATAC ....................................................................................................................................................................................................................................................GTTATAACTATTCGACGAAATAGTTATAAC................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 71 HP_score: -3.4 Tail_Score: -5.04249 Start: 432 End: 464 Full_Region: TTCTCTTTATAAGAA CGAGGATGTTTC ATTCTTTTC GATACACCCTCG TTTGTTTTTTGCACC ...............................................................................................................................................................................................................................................................................................................................................................................................................................................CGAGGATGTTTCATTCTTTTCGATACACCCTCG................................................... TransTerm Strand: - Conf: 64 HP_score: -6.3 Tail_Score: -3.78934 Start: 432 End: 464 Full_Region: ggtgcaaaaaacaaa cgagggtgtatcg aaaagaa tgaaacatcctcg ttcttataaagagaa ...............................................................................................................................................................................................................................................................................................................................................................................................................................................cgagggtgtatcgaaaagaatgaaacatcctcg................................................... Find igs database================================================ Query_seq: PG0896:PG0897|PG0896:PG0897:beta-galactosidase:alpha-amylase family protein:-><-:960443..960957 515 atgtgtccgcagctacttcgaatgcgaagagaacgggacattaattctctttcggttgaagccggaacggcattggtttattttccaatcttcttgaagtatagacacaaaagcattgaaaattcttcgaaatactcctaagctttctgcggaatactttgaactattcggtgatgcggtttgaactgttcggggaaatagtttgaactatccggcaaaatagttataactatccggtgaaatagttataactattcgacgaaatagttataactattcagtcaaatactttgatctattccgcgaagagcacagaagtatttgagagaaggcttattcggagccgccttgaaggaaaagttttcagatttggaaaactaaaattcctactgatcatatgctcaaacagcatacgattctctttataagaacgaggatgtttcattcttttcgatacaccctcgtttgttttttgcacccgttgaaagagctataggagaattggcttgatccgt Intra-Species Hit: Count: 1 Min: 1 Max: 515 Len: 515 Subject: NC_002950_PG0896_PG0897|beta-galactosidase:alpha-amylase family protein|POSITIVE:NEGATIVE|[960443,960957]|515 HSP 1 e-value: 0.0 bit: 1021.0 Len: 515 Query Start:1 Query End:515 Subject Strand: POSITIVE Subject Start: 1 Subject End: 515 atgtgtccgcagctacttcgaatgcgaagagaacgggacattaattctctttcggttgaagccggaacggcattggtttattttccaatcttcttgaagtatagacacaaaagcattgaaaattcttcgaaatactcctaagctttctgcggaatactttgaactattcggtgatgcggtttgaactgttcggggaaatagtttgaactatccggcaaaatagttataactatccggtgaaatagttataactattcgacgaaatagttataactattcagtcaaatactttgatctattccgcgaagagcacagaagtatttgagagaaggcttattcggagccgccttgaaggaaaagttttcagatttggaaaactaaaattcctactgatcatatgctcaaacagcatacgattctctttataagaacgaggatgtttcattcttttcgatacaccctcgtttgttttttgcacccgttgaaagagctataggagaattggcttgatccgt atgtgtccgcagctacttcgaatgcgaagagaacgggacattaattctctttcggttgaagccggaacggcattggtttattttccaatcttcttgaagtatagacacaaaagcattgaaaattcttcgaaatactcctaagctttctgcggaatactttgaactattcggtgatgcggtttgaactgttcggggaaatagtttgaactatccggcaaaatagttataactatccggtgaaatagttataactattcgacgaaatagttataactattcagtcaaatactttgatctattccgcgaagagcacagaagtatttgagagaaggcttattcggagccgccttgaaggaaaagttttcagatttggaaaactaaaattcctactgatcatatgctcaaacagcatacgattctctttataagaacgaggatgtttcattcttttcgatacaccctcgtttgttttttgcacccgttgaaagagctataggagaattggcttgatccgt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUGUGUCCGCAGCUACUUCGAAUGCGAAGAGAACGGGACAUUAAUUCUCUUUCGGUUGAAGCCGGAACGGCAUUGGUUUAUUUUCCAAUCUUCUUGAAGUAUAGACACAAAAGCAUUGAAAAUUCUUCGAAAUACUCCUAAGCUUUCUGCGGAAUACUUUGAACUAUUCGGUGAUGCGGUUUGAACUGUUCGGGGAAAUAGUUUGAACUAUCCGGCAAAAUAGUUAUAACUAUCCGGUGAAAUAGUUAUAACUAUUCGACGAAAUAGUUAUAACUAUUCAGUCAAAUACUUUGAUCUAUUCCGCGAAGAGCACAGAAGUAUUUGAGAGAAGGCUUAUUCGGAGCCGCCUUGAAGGAAAAGUUUUCAGAUUUGGAAAACUAAAAUUCCUACUGAUCAUAUGCUCAAACAGCAUACGAUUCUCUUUAUAAGAACGAGGAUGUUUCAUUCUUUUCGAUACACCCUCGUUUGUUUUUUGCACCCGUUGAAAGAGCUAUAGGAGAAUUGGCUUGAUCCGU .((((((......((((((((....(((((....((((.((((((.((.(((((((....))))))).)).)))))).....))))..)))))))))))))..)))))).............(((((..(((((((.((..(((((.(((((((((..(..((.((((...((((..((((..(((((((......)))))))..))))..((((....(((((....))))))))).(((.((((((((((((((......))))))))))))))))).)))))))).))..)..))))))))).)))))..)).)))))))..)))))(((((.....))))).......(((((.(((((((.......)))))))....)))))...(((((((((((.....))))))((((((((((.....((((((((.(((.((.........)).))).))))))))(((((((.((.....)))))))))...))))))))))...)))))... (-152.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACGGAUCAAGCCAAUUCUCCUAUAGCUCUUUCAACGGGUGCAAAAAACAAACGAGGGUGUAUCGAAAAGAAUGAAACAUCCUCGUUCUUAUAAAGAGAAUCGUAUGCUGUUUGAGCAUAUGAUCAGUAGGAAUUUUAGUUUUCCAAAUCUGAAAACUUUUCCUUCAAGGCGGCUCCGAAUAAGCCUUCUCUCAAAUACUUCUGUGCUCUUCGCGGAAUAGAUCAAAGUAUUUGACUGAAUAGUUAUAACUAUUUCGUCGAAUAGUUAUAACUAUUUCACCGGAUAGUUAUAACUAUUUUGCCGGAUAGUUCAAACUAUUUCCCCGAACAGUUCAAACCGCAUCACCGAAUAGUUCAAAGUAUUCCGCAGAAAGCUUAGGAGUAUUUCGAAGAAUUUUCAAUGCUUUUGUGUCUAUACUUCAAGAAGAUUGGAAAAUAAACCAAUGCCGUUCCGGCUUCAACCGAAAGAGAAUUAAUGUCCCGUUCUCUUCGCAUUCGAAGUAGCUGCGGACACAU .((((....(((..(((((.....((.(((.....))).))........(((((((((((.(((.......))).)))))))))))........)))))(((((((((.....)))))))))...(.(((((....(((((((.......))))))).))))).)..)))...))))........((((.(((((((((((((.(((.(((((((((((..((((.....))))..((((((((..(((((((..((.(((((((((((((((((......))))))))))))))))).))..)))))))..))))))))....((((.((((.............)))).))))....)))))))).)))))).))))))))))).)))))).............(((((((.((((((..((((.((((((.((......))....))))))))))...((((.((((((.........)))))))))).....)))))).....))))))). (-157.32) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 368 350 302 97 32 NC_002950:PG1388|5end_hypothetical_protein_1470889..1471119_NEGATIVE 356 198 155 68 20 NC_002950:PG0618|5end_alkyl_hydroperoxide_reductase,_C_subunit_670372..670602_POSITIVE 343 469 429 101 64 NC_002950:PG0608|5end_hypothetical_protein_662945..663175_POSITIVE 338 79 35 155 122 NC_002950:PG1536|5end_cell_division_protein_FtsX,_putative_1616362..1616592_POSITIVE 338 70 21 45 3 NC_002950:PG1521|5end_O-succinylbenzoic_acid--CoA_ligase_1599103..1599333_NEGATIVE 335 70 24 231 183 NC_002950:PG1402|5end_AP_endonuclease_domain_protein_1485963..1486193_NEGATIVE 330 209 167 217 173 NC_002950:PG0285|5end_hypothetical_protein_318459..318689_POSITIVE 329 49 1 161 110 NC_002950:PG0739|5end_ribonuclease_Z_786947..787177_POSITIVE 329 79 34 210 164 NC_002950:PG0474|5end_low-specificity_L-threonine_aldolase_516048..516278_NEGATIVE 326 476 432 229 184 NC_002950:PG1739|5end_hypothetical_protein_1825709..1825939_NEGATIVE 326 492 451 223 162 NC_002950:PG1281|5end_hypothetical_protein_1358848..1359078_POSITIVE 324 499 451 224 159 NC_002950:PG0351|5end_hypothetical_protein_386774..387004_POSITIVE 323 190 148 59 19 NC_002950:PG1294|5end_ferrous_iron_transport_protein_B_1372047..1372277_POSITIVE 321 216 171 87 40 NC_002950:PG0052|5end_sensor_histidine_kinase_63419..63649_POSITIVE 320 348 303 214 156 NC_002950:PG1939|5end_30S_ribosomal_protein_S10_2025081..2025311_NEGATIVE 318 350 304 178 125 NC_002950:PG0650|5end_hypothetical_protein_702964..703194_NEGATIVE 316 48 2 57 8 NC_002950:PG1859|5end_glycerate_kinase_family_protein_1958334..1958564_NEGATIVE 314 350 302 72 34 NC_002950:PG0324|5end_histidine_ammonia-lyase_359082..359312_NEGATIVE 313 77 31 103 56 NC_002950:PG0140|5end_hypothetical_protein_163858..164088_NEGATIVE 310 350 302 100 37 NC_002950:PG0789|5end_hypothetical_protein_841418..841648_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 368 350 302 116 51 NC_002950:PG1389|3end_DNA-binding_protein,_histone-like_family_1470870..1471020_NEGATIVE 357 349 303 109 54 NC_002950:PG0412|3end_DNA_mismatch_repair_protein_MutL_449584..449734_NEGATIVE 356 198 155 54 6 NC_002950:PG0617|3end_hypothetical_protein_670438..670588_POSITIVE 351 367 325 150 103 NC_002950:PG0199|3end_TatD_family_protein_237424..237574_NEGATIVE 344 470 421 63 1 NC_002950:PG0553|3end_extracellular_protease,_putative_608367..608517_NEGATIVE 343 469 429 70 33 NC_002950:PG0607|3end_hypothetical_protein_662994..663144_POSITIVE 341 206 168 128 91 NC_002950:PG1741|3end_aspartate_ammonia-lyase_1825856..1826006_NEGATIVE 338 70 21 48 6 NC_002950:PG1522|3end_mandelate_racemase/muconate_lactonizing_enzyme_family_protein_1599100..1599250_NEGATIVE 329 49 1 129 78 NC_002950:PG0738|3end_cytidine/deoxycytidylate_deaminase_family_protein_786995..787145_POSITIVE 326 492 451 96 35 NC_002950:PG1280|3end_hypothetical_protein_1358801..1358951_POSITIVE 321 356 315 51 1 NC_002950:PG1118|3end_clpB_protein_1192083..1192233_NEGATIVE 319 70 22 150 79 NC_002950:PG0229|3end_hypothetical_protein_265350..265500_POSITIVE 318 350 304 71 18 NC_002950:PG0651|3end_HDIG_domain_protein_703071..703221_NEGATIVE 317 348 301 72 21 NC_002950:PG1943|3end_hypothetical_protein_2028974..2029124_NEGATIVE 317 196 151 110 54 NC_002950:PG1720|3end_hypothetical_protein_1810224..1810374_POSITIVE 314 350 302 69 31 NC_002950:PG0325|3end_hypothetical_protein_359085..359235_NEGATIVE 313 77 31 83 36 NC_002950:PG0141|3end_spoOJ_protein_163878..164028_NEGATIVE 312 87 45 58 13 NC_002950:PG1330|3end_large_conductance_mechanosensitive_channel_protein_1410165..1410315_POSITIVE 310 350 302 119 56 NC_002950:PG0790|3end_GTP-binding_protein_Obg_841399..841549_NEGATIVE 309 55 11 77 32 NC_002950:PG0261|3end_ISPg3,_transposase_293862..294012_NEGATIVE