Origin IGS: gcataagaaagagaatgagtagacttggatagtttagagttaagtggcacgattgttaagtattgatgccatggatacacagacgagtatagccaaacggattgccgaaaccgattaggtattgtatctgaaggggagatagtccatacagcgattggcaacgatcatggcagagtcgaggagtggtgctacacagcttcgtcggtcgaataaattttgccggatttggagagttatcgccggagcaggcttttgaggctcattcgagtagattgcaaagtgcatcaggagtaaagagagcaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttgctctctttactcctgatgcactttgcaatctactcgaatgagcctcaaaagcctgctccggcgataactctccaaatccggcaaaatttattcgaccgacgaagctgtgtagcaccactcctcgactctgccatgatcgttgccaatcgctgtatggactatctccccttcagatacaatacctaatcggtttcggcaatccgtttggctatactcgtctgtgtatccatggcatcaatacttaacaatcgtgccacttaactctaaactatccaagtctactcattctctttcttatgc Mask Tandem Repeat Region ================================================ gcataagaaagagaatgagtagacttggatagtttagagttaagtggcacgattgttaagtattgatgccatggatacacagacgagtatagccaaacggattgccgaaaccgattaggtattgtatctgaaggggagatagtccatacagcgattggcaacgatcatggcagagtcgaggagtggtgctacacagcttcgtcggtcgaataaattttgccggatttggagagttatcgccggagcaggcttttgaggctcattcgagtagattgcaaagtgcatcaggagtaaagagagcaa Find is-nt database================================================ Query_seq: PG0886:PG0888|PG0886:PG0888:hypothetical protein:hypothetical protein:->->:949747..950049 303 gcataagaaagagaatgagtagacttggatagtttagagttaagtggcacgattgttaagtattgatgccatggatacacagacgagtatagccaaacggattgccgaaaccgattaggtattgtatctgaaggggagatagtccatacagcgattggcaacgatcatggcagagtcgaggagtggtgctacacagcttcgtcggtcgaataaattttgccggatttggagagttatcgccggagcaggcttttgaggctcattcgagtagattgcaaagtgcatcaggagtaaagagagcaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0886:PG0888|PG0886:PG0888:hypothetical protein:hypothetical protein:->->:949747..950049 303 gcataagaaagagaatgagtagacttggatagtttagagttaagtggcacgattgttaagtattgatgccatggatacacagacgagtatagccaaacggattgccgaaaccgattaggtattgtatctgaaggggagatagtccatacagcgattggcaacgatcatggcagagtcgaggagtggtgctacacagcttcgtcggtcgaataaattttgccggatttggagagttatcgccggagcaggcttttgaggctcattcgagtagattgcaaagtgcatcaggagtaaagagagcaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0886:PG0888|PG0886:PG0888:hypothetical protein:hypothetical protein:->->:949747..950049 303 gcataagaaagagaatgagtagacttggatagtttagagttaagtggcacgattgttaagtattgatgccatggatacacagacgagtatagccaaacggattgccgaaaccgattaggtattgtatctgaaggggagatagtccatacagcgattggcaacgatcatggcagagtcgaggagtggtgctacacagcttcgtcggtcgaataaattttgccggatttggagagttatcgccggagcaggcttttgaggctcattcgagtagattgcaaagtgcatcaggagtaaagagagcaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gcataagaaagagaatgagtagacttggatagtttagagttaagtggcacgattgttaagtattgatgccatggatacacagacgagtatagccaaacggattgccgaaaccgattaggtattgtatctgaaggggagatagtccatacagcgattggcaacgatcatggcagagtcgaggagtggtgctacacagcttcgtcggtcgaataaattttgccggatttggagagttatcgccggagcaggcttttgaggctcattcgagtagattgcaaagtgcatcaggagtaaagagagcaa Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 121 End: 210 ........................................................................................................................ M V S E G E I V H T A I G N D H G R V E E W C Y T A S S V E ............................................................................................. Protein_Len: 36 Strand: + Start: 146 End: 253 ................................................................................................................................................. M Q R L A T I M A E S R S G A T Q L R R S N K F C R I W R V I A G A G F .................................................. Protein_Len: 40 Strand: + Start: 183 End: 302 ...................................................................................................................................................................................... M V L H S F V G R I N F A G F G E L S P E Q A F E A H S S R L Q S A S G V K R A . Protein_Len: 60 Strand: - Start: 118 End: 303 ..................................................................................................................... Y R F P L Y D M C R N A V I M A S D L L P A V C S R R D F L N Q R I Q L T I A P A P K Q P E N S Y I A F H M L L L S L M Protein_Len: 51 Strand: - Start: 59 End: 211 .......................................................... T N I G H I C L R T Y G F P N G F G I L Y Q I Q L P S L G Y L S Q C R D H C L R P T T S C L K T P R M ............................................................................................ Protein_Len: 53 Strand: - Start: 3 End: 161 .. Y S L S H T S K S L K S N L P V I T L Y Q H W P Y V S S Y L W V S Q R F R N P I T D S P S I T W V A I P M .............................................................................................................................................. gcataagaaagagaatgagtagacttggatagtttagagttaagtggcacgattgttaagtattgatgccatggatacacagacgagtatagccaaacggattgccgaaaccgattaggtattgtatctgaaggggagatagtccatacagcgattggcaacgatcatggcagagtcgaggagtggtgctacacagcttcgtcggtcgaataaattttgccggatttggagagttatcgccggagcaggcttttgaggctcattcgagtagattgcaaagtgcatcaggagtaaagagagcaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 31 End: 60 ..............................CTTAACAATCGTGCCACTTAACTCTAAACT................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gcataagaaagagaatgagtagacttggatagtttagagttaagtggcacgattgttaagtattgatgccatggatacacagacgagtatagccaaacggattgccgaaaccgattaggtattgtatctgaaggggagatagtccatacagcgattggcaacgatcatggcagagtcgaggagtggtgctacacagcttcgtcggtcgaataaattttgccggatttggagagttatcgccggagcaggcttttgaggctcattcgagtagattgcaaagtgcatcaggagtaaagagagcaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0886:PG0888|PG0886:PG0888:hypothetical protein:hypothetical protein:->->:949747..950049 303 gcataagaaagagaatgagtagacttggatagtttagagttaagtggcacgattgttaagtattgatgccatggatacacagacgagtatagccaaacggattgccgaaaccgattaggtattgtatctgaaggggagatagtccatacagcgattggcaacgatcatggcagagtcgaggagtggtgctacacagcttcgtcggtcgaataaattttgccggatttggagagttatcgccggagcaggcttttgaggctcattcgagtagattgcaaagtgcatcaggagtaaagagagcaa Intra-Species Hit: Count: 1 Min: 1 Max: 303 Len: 303 Subject: NC_002950_PG0886_PG0888|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[949747,950049]|303 HSP 1 e-value: 1.0E-171 bit: 601.0 Len: 303 Query Start:1 Query End:303 Subject Strand: POSITIVE Subject Start: 1 Subject End: 303 gcataagaaagagaatgagtagacttggatagtttagagttaagtggcacgattgttaagtattgatgccatggatacacagacgagtatagccaaacggattgccgaaaccgattaggtattgtatctgaaggggagatagtccatacagcgattggcaacgatcatggcagagtcgaggagtggtgctacacagcttcgtcggtcgaataaattttgccggatttggagagttatcgccggagcaggcttttgaggctcattcgagtagattgcaaagtgcatcaggagtaaagagagcaa gcataagaaagagaatgagtagacttggatagtttagagttaagtggcacgattgttaagtattgatgccatggatacacagacgagtatagccaaacggattgccgaaaccgattaggtattgtatctgaaggggagatagtccatacagcgattggcaacgatcatggcagagtcgaggagtggtgctacacagcttcgtcggtcgaataaattttgccggatttggagagttatcgccggagcaggcttttgaggctcattcgagtagattgcaaagtgcatcaggagtaaagagagcaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCAUAAGAAAGAGAAUGAGUAGACUUGGAUAGUUUAGAGUUAAGUGGCACGAUUGUUAAGUAUUGAUGCCAUGGAUACACAGACGAGUAUAGCCAAACGGAUUGCCGAAACCGAUUAGGUAUUGUAUCUGAAGGGGAGAUAGUCCAUACAGCGAUUGGCAACGAUCAUGGCAGAGUCGAGGAGUGGUGCUACACAGCUUCGUCGGUCGAAUAAAUUUUGCCGGAUUUGGAGAGUUAUCGCCGGAGCAGGCUUUUGAGGCUCAUUCGAGUAGAUUGCAAAGUGCAUCAGGAGUAAAGAGAGCAA ((..........((((((((...((..((.((((((..((...((((((((((........)))).))))))....))...(((.......((((...((((((.(....((..(((((((...))))))).))...).)))))).......(((((....))))).))))...(.(((((.(((......)))..))))).).)))...))))))..(((...(((((.((....)).)))))...))).))..)))))))))).....(((.((.....))))).............)).. (-83.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGCUCUCUUUACUCCUGAUGCACUUUGCAAUCUACUCGAAUGAGCCUCAAAAGCCUGCUCCGGCGAUAACUCUCCAAAUCCGGCAAAAUUUAUUCGACCGACGAAGCUGUGUAGCACCACUCCUCGACUCUGCCAUGAUCGUUGCCAAUCGCUGUAUGGACUAUCUCCCCUUCAGAUACAAUACCUAAUCGGUUUCGGCAAUCCGUUUGGCUAUACUCGUCUGUGUAUCCAUGGCAUCAAUACUUAACAAUCGUGCCACUUAACUCUAAACUAUCCAAGUCUACUCAUUCUCUUUCUUAUGC .................(((((((............(((((((((........(((......))).........((......)).....)))))))))..(((((....(((((((...((....(((.((......)))))(((((.....(((...(((.....)))....))).......(((.....)))...)))))...))...)))))))))))).)))))))..((((((...............))))))............................................ (-55.86) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 399 180 139 230 183 NC_002950:PG1555|5end_hypothetical_protein_1636011..1636241_POSITIVE 389 286 243 160 106 NC_002950:PG2164|5end_peptidyl-prolyl_cis-trans_isomerase,_FKBP-type_2270125..2270355_NEGATIVE 388 234 200 28 1 NC_002950:PG1007|5end_transcriptional_regulator,_GntR_family_1066889..1067119_NEGATIVE 377 250 201 173 120 NC_002950:PG0223|5end_exonuclease_257610..257840_NEGATIVE 376 197 151 46 3 NC_002950:PG0842|5end_mobilizable_transposon,_hypothetical_protein,_putative_904874..905104_NEGATIVE 373 256 215 229 176 NC_002950:PG1383|5end_amino_acid_exporter,_putative_1462669..1462899_POSITIVE 372 240 194 88 43 NC_002950:PG1145|5end_long-chain-fatty-acid--CoA_ligase,_putative_1230305..1230535_NEGATIVE 371 208 161 200 156 NC_002950:PG2100|5end_immunoreactive_63_kDa_antigen_PG102_2210191..2210421_NEGATIVE 370 259 217 68 16 NC_002950:PG0753|5end_protease_798301..798531_POSITIVE 367 210 162 55 8 NC_002950:PG1414|5end_hypothetical_protein_1497009..1497239_POSITIVE 367 186 145 179 135 NC_002950:PG0401|5end_hypothetical_protein_439306..439536_NEGATIVE 366 266 221 190 145 NC_002950:PG2107|5end_thiamine_biosynthesis_protein_ThiH_2216658..2216888_NEGATIVE 365 266 221 144 101 NC_002950:PG1635|5end_hypothetical_protein_1715861..1716091_NEGATIVE 363 210 165 182 142 NC_002950:PG0724|5end_prolyl_oligopeptidase_family_protein_775229..775459_POSITIVE 360 209 161 185 122 NC_002950:PG1993|5end_excinuclease_ABC,_C_subunit_2083321..2083551_POSITIVE 360 209 162 123 80 NC_002950:PG1351|5end_hypothetical_protein_1432744..1432974_NEGATIVE 356 193 151 44 3 NC_002950:PG1556|5end_hypothetical_protein_1636595..1636825_POSITIVE 355 209 167 196 147 NC_002950:PG1959|5end_ribosomal_protein_L33_2048405..2048635_NEGATIVE 354 176 132 86 40 NC_002950:PG1982|5end_CRISPR-associated_protein_Cas1_2072620..2072850_NEGATIVE 354 268 221 226 172 NC_002950:PG0691|5end_NifU-related_protein_747469..747699_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 413 234 198 31 2 NC_002950:PG1008|3end_hypothetical_protein_1066886..1067036_NEGATIVE 379 139 91 134 67 NC_002950:PG0532|3end_hypothetical_protein_581249..581399_NEGATIVE 376 197 151 49 6 NC_002950:PG0843|3end_hypothetical_protein_904871..905021_NEGATIVE 376 240 194 125 83 NC_002950:PG0628|3end_ABC_transporter,_ATP-binding_protein_681684..681834_NEGATIVE 371 208 161 113 69 NC_002950:PG2101|3end_hypothetical_protein_2210278..2210428_NEGATIVE 370 259 217 84 32 NC_002950:PG0752|3end_uracil_phosphoribosyltransferase_798397..798547_POSITIVE 370 178 133 141 83 NC_002950:PG0740|3end_NLP/P60_family_protein_788485..788635_POSITIVE 367 277 243 148 109 NC_002950:PG2165|3end_glycyl-tRNA_synthetase_2270122..2270272_NEGATIVE 365 266 221 130 87 NC_002950:PG1636|3end_FtsK/SpoIIIE_family_protein_1715875..1716025_NEGATIVE 364 250 201 144 101 NC_002950:PG1012|3end_tRNA-i(6)A37_modification_enzyme_MiaB_1070089..1070239_NEGATIVE 360 260 219 144 97 NC_002950:PG2149|3end_hypothetical_protein_2256936..2257086_NEGATIVE 360 209 161 151 88 NC_002950:PG1992|3end_glucose-inhibited_division_protein_A_2083367..2083517_POSITIVE 360 209 162 130 87 NC_002950:PG1352|3end_hypothetical_protein_1432737..1432887_NEGATIVE 355 251 217 151 109 NC_002950:PG0957|3end_riboflavin_biosynthesis_protein_RibF_1018131..1018281_POSITIVE 354 209 163 59 12 NC_002950:PG2212|3end_hypothetical_protein_2324483..2324633_NEGATIVE 352 209 161 141 86 NC_002950:PG1403|3end_rhomboid_family_protein_1486173..1486323_NEGATIVE 352 270 222 131 73 NC_002950:PG1230|3end_hypothetical_protein_1305960..1306110_NEGATIVE 351 266 223 87 35 NC_002950:PG0380|3end_excinuclease_ABC_subunit_B_411218..411368_POSITIVE 350 208 162 112 49 NC_002950:PG0222|3end_DNA-binding_protein,_histone-like_family_255913..256063_NEGATIVE 347 208 170 85 41 NC_002950:PG0459|3end_ISPg5,_transposase_Orf1_501517..501667_NEGATIVE