Origin IGS: taagccttctcgtataaaacaacccttacttaccacgatgcaatatatttacctctctatcaattaggcgattagccttttaatcggtacaaataccccttaaataaatggggaaacagttttatctcactgttttataaggatttacatacttgtaccgcatggcttagttctcagtcttttaatcttcttttgggtgttgcactctataaatcaataga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tctattgatttatagagtgcaacacccaaaagaagattaaaagactgagaactaagccatgcggtacaagtatgtaaatccttataaaacagtgagataaaactgtttccccatttatttaaggggtatttgtaccgattaaaaggctaatcgcctaattgatagagaggtaaatatattgcatcgtggtaagtaagggttgttttatacgagaaggctta Mask Tandem Repeat Region ================================================ taagccttctcgtataaaacaacccttacttaccacgatgcaatatatttacctctctatcaattaggcgattagccttttaatcggtacaaataccccttaaataaatggggaaacagttttatctcactgttttataaggatttacatacttgtaccgcatggcttagttctcagtcttttaatcttcttttgggtgttgcactctataaatcaataga Find is-nt database================================================ Query_seq: PG0854:PG0855|PG0854:PG0855:hypothetical protein:hypothetical protein:-><-:915829..916049 221 taagccttctcgtataaaacaacccttacttaccacgatgcaatatatttacctctctatcaattaggcgattagccttttaatcggtacaaataccccttaaataaatggggaaacagttttatctcactgttttataaggatttacatacttgtaccgcatggcttagttctcagtcttttaatcttcttttgggtgttgcactctataaatcaataga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0854:PG0855|PG0854:PG0855:hypothetical protein:hypothetical protein:-><-:915829..916049 221 taagccttctcgtataaaacaacccttacttaccacgatgcaatatatttacctctctatcaattaggcgattagccttttaatcggtacaaataccccttaaataaatggggaaacagttttatctcactgttttataaggatttacatacttgtaccgcatggcttagttctcagtcttttaatcttcttttgggtgttgcactctataaatcaataga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0854:PG0855|PG0854:PG0855:hypothetical protein:hypothetical protein:-><-:915829..916049 221 taagccttctcgtataaaacaacccttacttaccacgatgcaatatatttacctctctatcaattaggcgattagccttttaatcggtacaaataccccttaaataaatggggaaacagttttatctcactgttttataaggatttacatacttgtaccgcatggcttagttctcagtcttttaatcttcttttgggtgttgcactctataaatcaataga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagccttctcgtataaaacaacccttacttaccacgatgcaatatatttacctctctatcaattaggcgattagccttttaatcggtacaaataccccttaaataaatggggaaacagttttatctcactgttttataaggatttacatacttgtaccgcatggcttagttctcagtcttttaatcttcttttgggtgttgcactctataaatcaataga Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 124 End: 219 ........................................................................................................................... M S L F Y K D L H T C T A W L S S Q S F N L L L G V A L Y K S I .. Protein_Len: 39 Strand: + Start: 104 End: 217 ....................................................................................................... M N G E T V L S H C F I R I Y I L V P H G L V L S L L I F F W V L H S I N Q * .... taagccttctcgtataaaacaacccttacttaccacgatgcaatatatttacctctctatcaattaggcgattagccttttaatcggtacaaataccccttaaataaatggggaaacagttttatctcactgttttataaggatttacatacttgtaccgcatggcttagttctcagtcttttaatcttcttttgggtgttgcactctataaatcaataga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagccttctcgtataaaacaacccttacttaccacgatgcaatatatttacctctctatcaattaggcgattagccttttaatcggtacaaataccccttaaataaatggggaaacagttttatctcactgttttataaggatttacatacttgtaccgcatggcttagttctcagtcttttaatcttcttttgggtgttgcactctataaatcaataga Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0854:PG0855|PG0854:PG0855:hypothetical protein:hypothetical protein:-><-:915829..916049 221 taagccttctcgtataaaacaacccttacttaccacgatgcaatatatttacctctctatcaattaggcgattagccttttaatcggtacaaataccccttaaataaatggggaaacagttttatctcactgttttataaggatttacatacttgtaccgcatggcttagttctcagtcttttaatcttcttttgggtgttgcactctataaatcaataga Intra-Species Hit: Count: 1 Min: 1 Max: 221 Len: 221 Subject: NC_002950_PG0854_PG0855|hypothetical protein:hypothetical protein|POSITIVE:NEGATIVE|[915829,916049]|221 HSP 1 e-value: 1.0E-122 bit: 438.0 Len: 221 Query Start:1 Query End:221 Subject Strand: POSITIVE Subject Start: 1 Subject End: 221 taagccttctcgtataaaacaacccttacttaccacgatgcaatatatttacctctctatcaattaggcgattagccttttaatcggtacaaataccccttaaataaatggggaaacagttttatctcactgttttataaggatttacatacttgtaccgcatggcttagttctcagtcttttaatcttcttttgggtgttgcactctataaatcaataga taagccttctcgtataaaacaacccttacttaccacgatgcaatatatttacctctctatcaattaggcgattagccttttaatcggtacaaataccccttaaataaatggggaaacagttttatctcactgttttataaggatttacatacttgtaccgcatggcttagttctcagtcttttaatcttcttttgggtgttgcactctataaatcaataga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGCCUUCUCGUAUAAAACAACCCUUACUUACCACGAUGCAAUAUAUUUACCUCUCUAUCAAUUAGGCGAUUAGCCUUUUAAUCGGUACAAAUACCCCUUAAAUAAAUGGGGAAACAGUUUUAUCUCACUGUUUUAUAAGGAUUUACAUACUUGUACCGCAUGGCUUAGUUCUCAGUCUUUUAAUCUUCUUUUGGGUGUUGCACUCUAUAAAUCAAUAGA ((((((.....(((............)))........((((........................((((.....)))).......(((((((...((((..........)))).((((((........))))))..................))))))))))))))))).........................(((((...))))).............. (-38.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUAUUGAUUUAUAGAGUGCAACACCCAAAAGAAGAUUAAAAGACUGAGAACUAAGCCAUGCGGUACAAGUAUGUAAAUCCUUAUAAAACAGUGAGAUAAAACUGUUUCCCCAUUUAUUUAAGGGGUAUUUGUACCGAUUAAAAGGCUAAUCGCCUAAUUGAUAGAGAGGUAAAUAUAUUGCAUCGUGGUAAGUAAGGGUUGUUUUAUACGAGAAGGCUUA (((((......)))))(((.(((((((.........(((......)))..(((..(((((((((((((((((..............(((((((........)))))))((((..........)))))))))))))))......((((.....)))).............((((.....))))..)))))).)))..))).)))).)))............. (-46.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 334 97 51 137 96 NC_002950:PG0002|5end_hexapeptide_transferase_family_protein_1295..1525_POSITIVE 331 209 171 217 168 NC_002950:PG0282|5end_ABC_transporter,_ATP-binding_protein_316915..317145_NEGATIVE 285 200 158 86 44 NC_002950:PG2140|5end_ribosomal_protein_L32_2246134..2246364_POSITIVE 284 46 3 75 32 NC_002950:PG1992|5end_glucose-inhibited_division_protein_A_2081410..2081640_POSITIVE 284 198 158 180 137 NC_002950:PG1778|5end_hypothetical_protein_1867054..1867284_POSITIVE 283 208 162 147 103 NC_002950:PG1226|5end_peptidyl-prolyl_cis-trans_isomerase,_cyclophilin-type_1303264..1303494_POSITIVE 283 207 165 169 119 NC_002950:PG0351|5end_hypothetical_protein_386774..387004_POSITIVE 281 199 152 203 158 NC_002950:PG0816|5end_hypothetical_protein_876859..877089_NEGATIVE 281 49 2 196 148 NC_002950:PG0790|5end_GTP-binding_protein_Obg_842583..842813_NEGATIVE 276 209 165 204 151 NC_002950:PG1185|5end_hypothetical_protein_1267752..1267982_NEGATIVE 273 206 162 131 95 NC_002950:PG2075|5end_hypothetical_protein_2176466..2176696_NEGATIVE 273 206 162 131 95 NC_002950:PG1671|5end_hypothetical_protein_1757658..1757888_NEGATIVE 273 206 162 131 95 NC_002950:PG1266|5end_hypothetical_protein_1346987..1347217_NEGATIVE 273 206 162 131 95 NC_002950:PG0101|5end_hypothetical_protein_119198..119428_POSITIVE 266 207 163 201 158 NC_002950:PG1809|5end_2-oxoglutarate_oxidoreductase,_gamma_subunit_1906036..1906266_NEGATIVE 265 200 159 70 30 NC_002950:PG1346|5end_glycosyl_transferase,_group_1_family_protein_1426708..1426938_POSITIVE 264 88 48 96 60 NC_002950:PG1619|5end_biotin_synthesis_protein_BioC,_putative_1698568..1698798_POSITIVE 260 200 158 75 27 NC_002950:PG1297|5end_ribosomal_protein_S1_1375831..1376061_POSITIVE 259 200 158 204 156 NC_002950:PG0165|5end_heat_shock_protein_15_187807..188037_NEGATIVE 258 196 152 133 85 NC_002950:PG1510|5end_hypothetical_protein_1587536..1587766_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 334 97 51 124 83 NC_002950:PG0001|3end_chromosomal_replication_initiation_protein_1362..1512_POSITIVE 331 209 171 137 88 NC_002950:PG0283|3end_efflux_transporter,_MFP_component,_RND_family_316995..317145_NEGATIVE 285 200 158 79 37 NC_002950:PG2139|3end_hypothetical_protein_2246207..2246357_POSITIVE 284 198 158 151 108 NC_002950:PG1777|3end_hypothetical_protein_1867105..1867255_POSITIVE 283 208 162 139 95 NC_002950:PG1225|3end_ABC_transporter,_ATP-binding_protein_1303336..1303486_POSITIVE 275 200 165 122 89 NC_002950:PG0548|3end_pyruvate_ferredoxin/flavodoxin_oxidoreductase_family_protein_605759..605909_POSITIVE 265 97 51 144 82 NC_002950:PG2216|3end_hypothetical_protein_2329530..2329680_POSITIVE 265 200 159 73 33 NC_002950:PG1345|3end_glycosyl_transferase,_group_1_family_protein_1426791..1426941_POSITIVE 264 88 48 117 81 NC_002950:PG1618|3end_hypothetical_protein_1698669..1698819_POSITIVE 263 196 158 52 13 NC_002950:PG1676|3end_phosphoenolpyruvate_carboxykinase_1760489..1760639_NEGATIVE 262 89 52 124 79 NC_002950:PG0403|3end_hypothetical_protein_439453..439603_NEGATIVE 258 196 151 89 48 NC_002950:PG1483|3end_conjugative_transposon_protein_TraE_1558783..1558933_NEGATIVE 257 90 51 48 17 NC_002950:PG1388|3end_hypothetical_protein_1470095..1470245_NEGATIVE 255 203 161 126 78 NC_002950:PG0124|3end_hypothetical_protein_145614..145764_NEGATIVE 254 200 159 121 67 NC_002950:PG1234|3end_hypothetical_protein_1310320..1310470_POSITIVE 254 196 152 89 49 NC_002950:PG1004|3end_prolyl_oligopeptidase_family_protein_1060433..1060583_NEGATIVE 254 207 163 133 87 NC_002950:PG0242|3end_conserved_hypothetical_protein_TIGR00096_278259..278409_NEGATIVE 252 199 152 115 48 NC_002950:PG1162|3end_ATP:cob(I)alamin_adenosyltransferase,_putative_1241473..1241623_NEGATIVE 250 169 121 116 60 NC_002950:PG1470|3end_hypothetical_protein_1548431..1548581_POSITIVE 248 168 121 145 97 NC_002950:PG0391|3end_50S_ribosomal_protein_L1_424058..424208_POSITIVE