Origin IGS: attgctatatggggcatagattttagggggattttccacaaagaatatctgctcactgccaaaaataacgaaatgctttttatcggatttctttgctcctttctttttcgccatttcccctgctcatgaaagaaagtagcggtggacaaatctgccgaccgctttactctgtctgttcttcttgaacggttacttgttccgtccttacttcggaataaatcatcttgtctgattttatgcggattccagaggtgagggagttgagtttcaaccttttcttttttccctgcttcttgcatatccaacattttttatgcgttttttccgtcggggcggtcgttctcgtttcaggggcgaaaaaaggtagggcttaggacaaacaaggttttacggtgagaatactacctgcaataaaatggaagtgtggagattgtcgtctaaacggcttgctgccccgaccttttttgtccgtagaagcctggaactaccttcgccgctgacaacgaacaaccgatcccgacatgatacacggcataagttgaggaagtgcagggagagaagcagggcgaacacaaaaaacgcagtctttaaagactgcatttaccataagaatagaatacacagaaaacaaaaagccgccctaacggacggttcatacgttcaaattgaatatagtatgagatgtttgtgaaaaagaagaaatgtacaaaaaaggttatacatttgcctaacattcagaggcaatagtcttatcgtctataccttggatgtgttcgaaacctttggatactatgatac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gtatcatagtatccaaaggtttcgaacacatccaaggtatagacgataagactattgcctctgaatgttaggcaaatgtataaccttttttgtacatttcttctttttcacaaacatctcatactatattcaatttgaacgtatgaaccgtccgttagggcggctttttgttttctgtgtattctattcttatggtaaatgcagtctttaaagactgcgttttttgtgttcgccctgcttctctccctgcacttcctcaacttatgccgtgtatcatgtcgggatcggttgttcgttgtcagcggcgaaggtagttccaggcttctacggacaaaaaaggtcggggcagcaagccgtttagacgacaatctccacacttccattttattgcaggtagtattctcaccgtaaaaccttgtttgtcctaagccctaccttttttcgcccctgaaacgagaacgaccgccccgacggaaaaaacgcataaaaaatgttggatatgcaagaagcagggaaaaaagaaaaggttgaaactcaactccctcacctctggaatccgcataaaatcagacaagatgatttattccgaagtaaggacggaacaagtaaccgttcaagaagaacagacagagtaaagcggtcggcagatttgtccaccgctactttctttcatgagcaggggaaatggcgaaaaagaaaggagcaaagaaatccgataaaaagcatttcgttatttttggcagtgagcagatattctttgtggaaaatccccctaaaatctatgccccatatagcaat Mask Tandem Repeat Region ================================================ attgctatatggggcatagattttagggggattttccacaaagaatatctgctcactgccaaaaataacgaaatgctttttatcggatttctttgctcctttctttttcgccatttcccctgctcatgaaagaaagtagcggtggacaaatctgccgaccgctttactctgtctgttcttcttgaacggttacttgttccgtccttacttcggaataaatcatcttgtctgattttatgcggattccagaggtgagggagttgagtttcaaccttttcttttttccctgcttcttgcatatccaacattttttatgcgttttttccgtcggggcggtcgttctcgtttcaggggcgaaaaaaggtagggcttaggacaaacaaggttttacggtgagaatactacctgcaataaaatggaagtgtggagattgtcgtctaaacggcttgctgccccgaccttttttgtccgtagaagcctggaactaccttcgccgctgacaacgaacaaccgatcccgacatgatacacggcataagttgaggaagtgcagggagagaagcagggcgaacacaaaaaacgcagtctttaaagactgcatttaccataagaatagaatacacagaaaacaaaaagccgccctaacggacggttcatacgttcaaattgaatatagtatgagatgtttgtgaaaaagaagaaatgtacaaaaaaggttatacatttgcctaacattcagaggcaatagtcttatcgtctataccttggatgtgttcgaaacctttggatactatgatac Find is-nt database================================================ Query_seq: PG0831:PG0832|PG0831:PG0832:hypothetical protein:hypothetical protein:-><-:887972..888769 798 attgctatatggggcatagattttagggggattttccacaaagaatatctgctcactgccaaaaataacgaaatgctttttatcggatttctttgctcctttctttttcgccatttcccctgctcatgaaagaaagtagcggtggacaaatctgccgaccgctttactctgtctgttcttcttgaacggttacttgttccgtccttacttcggaataaatcatcttgtctgattttatgcggattccagaggtgagggagttgagtttcaaccttttcttttttccctgcttcttgcatatccaacattttttatgcgttttttccgtcggggcggtcgttctcgtttcaggggcgaaaaaaggtagggcttaggacaaacaaggttttacggtgagaatactacctgcaataaaatggaagtgtggagattgtcgtctaaacggcttgctgccccgaccttttttgtccgtagaagcctggaactaccttcgccgctgacaacgaacaaccgatcccgacatgatacacggcataagttgaggaagtgcagggagagaagcagggcgaacacaaaaaacgcagtctttaaagactgcatttaccataagaatagaatacacagaaaacaaaaagccgccctaacggacggttcatacgttcaaattgaatatagtatgagatgtttgtgaaaaagaagaaatgtacaaaaaaggttatacatttgcctaacattcagaggcaatagtcttatcgtctataccttggatgtgttcgaaacctttggatactatgatac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0831:PG0832|PG0831:PG0832:hypothetical protein:hypothetical protein:-><-:887972..888769 798 attgctatatggggcatagattttagggggattttccacaaagaatatctgctcactgccaaaaataacgaaatgctttttatcggatttctttgctcctttctttttcgccatttcccctgctcatgaaagaaagtagcggtggacaaatctgccgaccgctttactctgtctgttcttcttgaacggttacttgttccgtccttacttcggaataaatcatcttgtctgattttatgcggattccagaggtgagggagttgagtttcaaccttttcttttttccctgcttcttgcatatccaacattttttatgcgttttttccgtcggggcggtcgttctcgtttcaggggcgaaaaaaggtagggcttaggacaaacaaggttttacggtgagaatactacctgcaataaaatggaagtgtggagattgtcgtctaaacggcttgctgccccgaccttttttgtccgtagaagcctggaactaccttcgccgctgacaacgaacaaccgatcccgacatgatacacggcataagttgaggaagtgcagggagagaagcagggcgaacacaaaaaacgcagtctttaaagactgcatttaccataagaatagaatacacagaaaacaaaaagccgccctaacggacggttcatacgttcaaattgaatatagtatgagatgtttgtgaaaaagaagaaatgtacaaaaaaggttatacatttgcctaacattcagaggcaatagtcttatcgtctataccttggatgtgttcgaaacctttggatactatgatac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0831:PG0832|PG0831:PG0832:hypothetical protein:hypothetical protein:-><-:887972..888769 798 attgctatatggggcatagattttagggggattttccacaaagaatatctgctcactgccaaaaataacgaaatgctttttatcggatttctttgctcctttctttttcgccatttcccctgctcatgaaagaaagtagcggtggacaaatctgccgaccgctttactctgtctgttcttcttgaacggttacttgttccgtccttacttcggaataaatcatcttgtctgattttatgcggattccagaggtgagggagttgagtttcaaccttttcttttttccctgcttcttgcatatccaacattttttatgcgttttttccgtcggggcggtcgttctcgtttcaggggcgaaaaaaggtagggcttaggacaaacaaggttttacggtgagaatactacctgcaataaaatggaagtgtggagattgtcgtctaaacggcttgctgccccgaccttttttgtccgtagaagcctggaactaccttcgccgctgacaacgaacaaccgatcccgacatgatacacggcataagttgaggaagtgcagggagagaagcagggcgaacacaaaaaacgcagtctttaaagactgcatttaccataagaatagaatacacagaaaacaaaaagccgccctaacggacggttcatacgttcaaattgaatatagtatgagatgtttgtgaaaaagaagaaatgtacaaaaaaggttatacatttgcctaacattcagaggcaatagtcttatcgtctataccttggatgtgttcgaaacctttggatactatgatac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attgctatatggggcatagattttagggggattttccacaaagaatatctgctcactgccaaaaataacgaaatgctttttatcggatttctttgctcctttctttttcgccatttcccctgctcatgaaagaaagtagcggtggacaaatctgccgaccgctttactctgtctgttcttcttgaacggttacttgttccgtccttacttcggaataaatcatcttgtctgattttatgcggattccagaggtgagggagttgagtttcaaccttttcttttttccctgcttcttgcatatccaacattttttatgcgttttttccgtcggggcggtcgttctcgtttcaggggcgaaaaaaggtagggcttaggacaaacaaggttttacggtgagaatactacctgcaataaaatggaagtgtggagattgtcgtctaaacggcttgctgccccgaccttttttgtccgtagaagcctggaactaccttcgccgctgacaacgaacaaccgatcccgacatgatacacggcataagttgaggaagtgcagggagagaagcagggcgaacacaaaaaacgcagtctttaaagactgcatttaccataagaatagaatacacagaaaacaaaaagccgccctaacggacggttcatacgttcaaattgaatatagtatgagatgtttgtgaaaaagaagaaatgtacaaaaaaggttatacatttgcctaacattcagaggcaatagtcttatcgtctataccttggatgtgttcgaaacctttggatactatgatac Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: + Start: 1 End: 126 M A I W G I D F R G I F H K E Y L L T A K N N E M L F I G F L C S F L F R H F P C S ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 39 Strand: + Start: 20 End: 136 ................... M L G G F S T K N I C S L P K I T K C F L S D F F A P F F F A I S P A H E R K ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 32 Strand: + Start: 87 End: 182 ...................................................................................... M S L L L S F S P F P L L M K E S S G G Q I C R P L Y S V C S S ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 37 Strand: + Start: 142 End: 252 ............................................................................................................................................. M D K S A D R F T L S V L L E R L L V P S L L R N K S S C L I L C G F Q R .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 51 Strand: + Start: 219 End: 371 .......................................................................................................................................................................................................................... M I L S D F M R I P E V R E L S F N L F F F P C F L H I Q H F L C V F S V G A V V L V S G A K K G R A ........................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 32 Strand: + Start: 298 End: 393 ......................................................................................................................................................................................................................................................................................................... M S N I F Y A F F P S G R S F S F Q G R K K V G L R T N K V L R ..................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 35 Strand: + Start: 393 End: 497 ........................................................................................................................................................................................................................................................................................................................................................................................................ M R I L P A I K W K C G D C R L N G L L P R P F L S V E A W N Y L R R ............................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 416 End: 688 ............................................................................................................................................................................................................................................................................................................................................................................................................................... M E V W R L S S K R L A A P T F F V R R S L E L P S P L T T N N R S R H D T R H K L R K C R E R S R A N T K N A V F K D .............................................................................................................. Protein_Len: 30 Strand: - Start: 664 End: 753 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... I S Y L I L H K H F L L F T C F L N Y M Q R V N L P L L R M ............................................. Protein_Len: 48 Strand: - Start: 591 End: 734 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. L S C K G Y S Y F V C F V F L R G L P R N M R E F Q I Y Y S I N T F F F F H V F F T I C K G L M ................................................................ Protein_Len: 48 Strand: - Start: 488 End: 631 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... R R R Q C R V V S G S M I C P M L Q P L A P L S A P R V C F V C D K F V A N V M L I S Y V S F M ....................................................................................................................................................................... Protein_Len: 48 Strand: - Start: 441 End: 584 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................ V A Q Q G S R K Q G Y F G P V V K A A S L S C G I G V H Y V A Y T S S T C P S F C P S C L F R M ...................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 391 End: 600 ...................................................................................................................................................................................................................................................................................................................................................................................................... T H L N D D L R S A A G V K K T R L L R S S G E G S V V F L R D R C S V R C L N L F H L S L L L A F V F F A T K L S Q M ...................................................................................................................................................................................................... Protein_Len: 45 Strand: - Start: 167 End: 301 ...................................................................................................................................................................... E T Q E E Q V T V Q E T R V E S Y I M K D S K I R I G S T L S N L K L R K K K G Q K K C M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 3 End: 308 .. F F T A T S L D A S R K V R D T R R S R N S T G D K S R F L D D Q R I K H P N W L H P L Q T E V K E K K G A E Q M D L M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 38 Strand: - Start: 1 End: 114 N S Y P A Y I K P P N E V F F I Q E S G F I V F H K K D S K K A G K K K A M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ attgctatatggggcatagattttagggggattttccacaaagaatatctgctcactgccaaaaataacgaaatgctttttatcggatttctttgctcctttctttttcgccatttcccctgctcatgaaagaaagtagcggtggacaaatctgccgaccgctttactctgtctgttcttcttgaacggttacttgttccgtccttacttcggaataaatcatcttgtctgattttatgcggattccagaggtgagggagttgagtttcaaccttttcttttttccctgcttcttgcatatccaacattttttatgcgttttttccgtcggggcggtcgttctcgtttcaggggcgaaaaaaggtagggcttaggacaaacaaggttttacggtgagaatactacctgcaataaaatggaagtgtggagattgtcgtctaaacggcttgctgccccgaccttttttgtccgtagaagcctggaactaccttcgccgctgacaacgaacaaccgatcccgacatgatacacggcataagttgaggaagtgcagggagagaagcagggcgaacacaaaaaacgcagtctttaaagactgcatttaccataagaatagaatacacagaaaacaaaaagccgccctaacggacggttcatacgttcaaattgaatatagtatgagatgtttgtgaaaaagaagaaatgtacaaaaaaggttatacatttgcctaacattcagaggcaatagtcttatcgtctataccttggatgtgttcgaaacctttggatactatgatac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attgctatatggggcatagattttagggggattttccacaaagaatatctgctcactgccaaaaataacgaaatgctttttatcggatttctttgctcctttctttttcgccatttcccctgctcatgaaagaaagtagcggtggacaaatctgccgaccgctttactctgtctgttcttcttgaacggttacttgttccgtccttacttcggaataaatcatcttgtctgattttatgcggattccagaggtgagggagttgagtttcaaccttttcttttttccctgcttcttgcatatccaacattttttatgcgttttttccgtcggggcggtcgttctcgtttcaggggcgaaaaaaggtagggcttaggacaaacaaggttttacggtgagaatactacctgcaataaaatggaagtgtggagattgtcgtctaaacggcttgctgccccgaccttttttgtccgtagaagcctggaactaccttcgccgctgacaacgaacaaccgatcccgacatgatacacggcataagttgaggaagtgcagggagagaagcagggcgaacacaaaaaacgcagtctttaaagactgcatttaccataagaatagaatacacagaaaacaaaaagccgccctaacggacggttcatacgttcaaattgaatatagtatgagatgtttgtgaaaaagaagaaatgtacaaaaaaggttatacatttgcctaacattcagaggcaatagtcttatcgtctataccttggatgtgttcgaaacctttggatactatgatac Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 80 HP_score: -2.8 Tail_Score: -5.43553 Start: 259 End: 273 Full_Region: TTCCAGAGGTGAGGG AGTTGA GTT TCAACC TTTTCTTTTTTCCCT ..................................................................................................................................................................................................................................................................AGTTGAGTTTCAACC............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 100 HP_score: -8.6 Tail_Score: -5.47159 Start: 580 End: 599 Full_Region: tattcttatggtaaa tgcagtct ttaa agactgcg ttttttgtgttcgcc ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgcagtctttaaagactgcg....................................................................................................................................................................................................... TransTerm Strand: + Conf: 72 HP_score: -9.9 Tail_Score: -2.75963 Start: 581 End: 598 Full_Region: GCGAACACAAAAAAC GCAGTCT TTAA AGACTGC ATTTACCATAAGAAT ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GCAGTCTTTAAAGACTGC........................................................................................................................................................................................................ TransTerm Strand: - Conf: 84 HP_score: -9.9 Tail_Score: -3.87211 Start: 581 End: 598 Full_Region: attcttatggtaaat gcagtct ttaa agactgc gttttttgtgttcgc ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gcagtctttaaagactgc........................................................................................................................................................................................................ TransTerm Strand: - Conf: 91 HP_score: -4.7 Tail_Score: -5.58522 Start: 635 End: 652 Full_Region: aatttgaacgtatga accgtcc gtta gggcggc tttttgttttctgtg ..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................accgtccgttagggcggc.................................................................................................................................................. TransTerm Strand: - Conf: 77 HP_score: -8.2 Tail_Score: -3.92673 Start: 636 End: 651 Full_Region: atttgaacgtatgaa ccgtcc gtta gggcgg ctttttgttttctgt ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ccgtccgttagggcgg................................................................................................................................................... Find igs database================================================ Query_seq: PG0831:PG0832|PG0831:PG0832:hypothetical protein:hypothetical protein:-><-:887972..888769 798 attgctatatggggcatagattttagggggattttccacaaagaatatctgctcactgccaaaaataacgaaatgctttttatcggatttctttgctcctttctttttcgccatttcccctgctcatgaaagaaagtagcggtggacaaatctgccgaccgctttactctgtctgttcttcttgaacggttacttgttccgtccttacttcggaataaatcatcttgtctgattttatgcggattccagaggtgagggagttgagtttcaaccttttcttttttccctgcttcttgcatatccaacattttttatgcgttttttccgtcggggcggtcgttctcgtttcaggggcgaaaaaaggtagggcttaggacaaacaaggttttacggtgagaatactacctgcaataaaatggaagtgtggagattgtcgtctaaacggcttgctgccccgaccttttttgtccgtagaagcctggaactaccttcgccgctgacaacgaacaaccgatcccgacatgatacacggcataagttgaggaagtgcagggagagaagcagggcgaacacaaaaaacgcagtctttaaagactgcatttaccataagaatagaatacacagaaaacaaaaagccgccctaacggacggttcatacgttcaaattgaatatagtatgagatgtttgtgaaaaagaagaaatgtacaaaaaaggttatacatttgcctaacattcagaggcaatagtcttatcgtctataccttggatgtgttcgaaacctttggatactatgatac Intra-Species Hit: Count: 2 Min: 1 Max: 798 Len: 798 Subject: NC_002950_PG0831_PG0832|hypothetical protein:hypothetical protein|POSITIVE:NEGATIVE|[887972,888769]|798 HSP 1 e-value: 0.0 bit: 938.0 Len: 473 Query Start:326 Query End:798 Subject Strand: POSITIVE Subject Start: 326 Subject End: 798 .....................................................................................................................................................................................................................................................................................................................................cgtcggggcggtcgttctcgtttcaggggcgaaaaaaggtagggcttaggacaaacaaggttttacggtgagaatactacctgcaataaaatggaagtgtggagattgtcgtctaaacggcttgctgccccgaccttttttgtccgtagaagcctggaactaccttcgccgctgacaacgaacaaccgatcccgacatgatacacggcataagttgaggaagtgcagggagagaagcagggcgaacacaaaaaacgcagtctttaaagactgcatttaccataagaatagaatacacagaaaacaaaaagccgccctaacggacggttcatacgttcaaattgaatatagtatgagatgtttgtgaaaaagaagaaatgtacaaaaaaggttatacatttgcctaacattcagaggcaatagtcttatcgtctataccttggatgtgttcgaaacctttggatactatgatac .....................................................................................................................................................................................................................................................................................................................................cgtcggggcggtcgttctcgtttcaggggcgaaaaaaggtagggcttaggacaaacaaggttttacggtgagaatactacctgcaataaaatggaagtgtggagattgtcgtctaaacggcttgctgccccgaccttttttgtccgtagaagcctggaactaccttcgccgctgacaacgaacaaccgatcccgacatgatacacggcataagttgaggaagtgcagggagagaagcagggcgaacacaaaaaacgcagtctttaaagactgcatttaccataagaatagaatacacagaaaacaaaaagccgccctaacggacggttcatacgttcaaattgaatatagtatgagatgtttgtgaaaaagaagaaatgtacaaaaaaggttatacatttgcctaacattcagaggcaatagtcttatcgtctataccttggatgtgttcgaaacctttggatactatgatac HSP 2 e-value: 1.0E-148 bit: 525.0 Len: 265 Query Start:1 Query End:265 Subject Strand: POSITIVE Subject Start: 1 Subject End: 265 attgctatatggggcatagattttagggggattttccacaaagaatatctgctcactgccaaaaataacgaaatgctttttatcggatttctttgctcctttctttttcgccatttcccctgctcatgaaagaaagtagcggtggacaaatctgccgaccgctttactctgtctgttcttcttgaacggttacttgttccgtccttacttcggaataaatcatcttgtctgattttatgcggattccagaggtgagggagttgag..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... attgctatatggggcatagattttagggggattttccacaaagaatatctgctcactgccaaaaataacgaaatgctttttatcggatttctttgctcctttctttttcgccatttcccctgctcatgaaagaaagtagcggtggacaaatctgccgaccgctttactctgtctgttcttcttgaacggttacttgttccgtccttacttcggaataaatcatcttgtctgattttatgcggattccagaggtgagggagttgag..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_002950_PG1436_PG1439|ATPase, putative:hypothetical protein|POSITIVE:NEGATIVE|[1523815,1524674]|860 HSP 1 e-value: 8.0E-12 bit: 73.8 Len: 93 Query Start:121 Query End:213 Subject Strand: NEGATIVE Subject Start: 655 Subject End: 747 ........................................................................................................................tgctcatgaaagaaagtagcggtggacaaatctgccgaccgctttactctgtctgttcttcttgaacggttacttgttccgtccttacttcgg......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ........................................................................................................................tgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcgg......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 8.0E-9 bit: 63.9 Len: 36 Query Start:230 Query End:265 Subject Strand: NEGATIVE Subject Start: 346 Subject End: 381 .....................................................................................................................................................................................................................................tgattttatgcggattccagaggtgagggagttgag..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... .....................................................................................................................................................................................................................................tgattttatgcggattccagaggagagggagttgag..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 3 Min: 121 Max: 651 Len: 531 Subject: pint_PI0282_PI0283|hypothetical protein:Zn-dependent peptidase|POSITIVE:POSITIVE|[266887,267693]|807 HSP 1 e-value: 1.0E-109 bit: 396.0 Len: 326 Query Start:326 Query End:651 Subject Strand: POSITIVE Subject Start: 108 Subject End: 434 .....................................................................................................................................................................................................................................................................................................................................cgtcggggcggtcgttctcgtttcaggggcgaaaaaaggtagggcttaggacaaacaaggttttacggtgagaatactacctgcaataaaatggaagtgtggagattgtcgtctaaacggcttgctgccccgaccttttttgtccgtagaagcctggaactaccttcgccgctgacaacgaacaaccgatcccgacatgatacac-ggcataagttgaggaagtgcagggagagaagcagggcgaacacaaaaaacgcagtctttaaagactgcat-ttaccataagaatagaatacacagaaaacaaaaagccgccctaacggacgg................................................................................................................................................... .....................................................................................................................................................................................................................................................................................................................................cgtcgggccggtcgttttcgtttcagtggcgtaaaaaggtagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtctaaacggcttgccgccccgaccttttttgtccgtagaagcccggaactacctttgccgctgacaacgaacaaccgatcccgacataatacacgggcataagtggaggatgtgcagacggagaagcggggcgaaaacaaaaaacgcagtcttcggggactgcatcttatcataa-aacggaatacacggaaaacaaaaagccgccccaacggacgg................................................................................................................................................... Subject: tfor_TF0934_TF0936|hypothetical protein:subtilisin-like serine protease|POSITIVE:POSITIVE|[975268,976007]|740 HSP 1 e-value: 3.0E-79 bit: 297.0 Len: 316 Query Start:326 Query End:641 Subject Strand: POSITIVE Subject Start: 112 Subject End: 427 .....................................................................................................................................................................................................................................................................................................................................cgtcggggcggtcgttctcgtttcaggggcg-aaaaaaggtagggcttaggacaaacaaggttttacggtgagaatactacctgcaataaaatggaagtgtggagattgtcgtctaaacggcttgctgccccgaccttttttgtccgtagaagcctggaactaccttcgccgctgacaacgaacaaccgatcccgacatgatacac-ggcataagttgaggaagtgcagggagagaagcagggcgaacacaaaaaacgcagtctttaaagactgcatttaccataagaatagaatacacagaaaacaaaaagccgccc............................................................................................................................................................. .....................................................................................................................................................................................................................................................................................................................................cgtcggggaagtctttttcgtttcagtggcgtaaaaaaaggagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtcgtaacggcttgccgctccgaccttttttgtccgtgaaagcccaaaactacctttgtcactgacaacgaacaaccgagcccgacatgatatacgggcataagtggaggatgtgcagggagagaagcaggac-aaaacaaaaaacacagtctttaaggactcattttatcataagaatagaatacaca-aaaacaaaaagctgccc............................................................................................................................................................. Subject: pint_PI0401_PI0402|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[367168,368031]|864 HSP 1 e-value: 8.0E-12 bit: 73.8 Len: 93 Query Start:121 Query End:213 Subject Strand: POSITIVE Subject Start: 117 Subject End: 209 ........................................................................................................................tgctcatgaaagaaagtagcggtggacaaatctgccgaccgctttactctgtctgttcttcttgaacggttacttgttccgtccttacttcgg......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ........................................................................................................................tgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcgg......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 8.0E-9 bit: 63.9 Len: 36 Query Start:230 Query End:265 Subject Strand: POSITIVE Subject Start: 481 Subject End: 516 .....................................................................................................................................................................................................................................tgattttatgcggattccagaggtgagggagttgag..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... .....................................................................................................................................................................................................................................tgattttatgcggattccagaggagagggagttgag..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGCUCAUGAAAGAAAGUAGCGGUGGACAAAUCUGCCGACCGCUUUACUCUGUCUGUUCUUCUUGAACGGUUACUUGUUCCGUCCUUACUUCGGAAUAAAUCAUCUUGUCUGAUUUUAUGCGGAUUCCAGAGGUGAGGGAGUUGAGUUUCAACCUUUUCUUUUUUCCCUGCUUCUUGCAUAUCCAACAUUUUUUAUGCGUUUUUUCCGUCGGGGCGGUCGUUCUCGUUUCAGGGGCGAAAAAAGGUAGGGCUUAGGACAAACAAGGUUUUACGGUGAGAAUACUACCUGCAAUAAAAUGGAAGUGUGGAGAUUGUCGUCUAAACGGCUUGCUGCCCCGACCUUUUUUGUCCGUAGAAGCCUGGAACUACCUUCGCCGCUGACAACGAACAACCGAUCCCGACAUGAUACACGGCAUAAGUUGAGGAAGUGCAGGGAGAGAAGCAGGGCGAACACAAAAAACGCAGUCUUUAAAGACUGCAUUUACCAUAAGAAUAGAAUACACAGAAAACAAAAAGCCGCCCUAACGGACGG ............((((..((((..((....))..))).)..))))..(((((.(((((((((((...(((....(((((..(((((((((((((((((((((.......)))))).......))))).)))))))))).((((.....))))............(((((((((((((((.(((..((...(((((((((....((.(((((((((((.((..(((((((((.(((((....(((((((((((.(((((((.((((((..((((..(..(((((.((...........)).))))).....)..))))......(((.....)))..)))))).)))))))....))))).....))))))))))).)))).))))))).)))).)))))))..))...)).))))))).)).))).))))).....)))))))))).)))))........(((((((....)))))))....))).)))))..)))))).)))))..........(((.((....)).))) (-188.81) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CCGUCCGUUAGGGCGGCUUUUUGUUUUCUGUGUAUUCUAUUCUUAUGGUAAAUGCAGUCUUUAAAGACUGCGUUUUUUGUGUUCGCCCUGCUUCUCUCCCUGCACUUCCUCAACUUAUGCCGUGUAUCAUGUCGGGAUCGGUUGUUCGUUGUCAGCGGCGAAGGUAGUUCCAGGCUUCUACGGACAAAAAAGGUCGGGGCAGCAAGCCGUUUAGACGACAAUCUCCACACUUCCAUUUUAUUGCAGGUAGUAUUCUCACCGUAAAACCUUGUUUGUCCUAAGCCCUACCUUUUUUCGCCCCUGAAACGAGAACGACCGCCCCGACGGAAAAAACGCAUAAAAAAUGUUGGAUAUGCAAGAAGCAGGGAAAAAAGAAAAGGUUGAAACUCAACUCCCUCACCUCUGGAAUCCGCAUAAAAUCAGACAAGAUGAUUUAUUCCGAAGUAAGGACGGAACAAGUAACCGUUCAAGAAGAACAGACAGAGUAAAGCGGUCGGCAGAUUUGUCCACCGCUACUUUCUUUCAUGAGCA ((((((....)))))).....((((((((((.(.((((..((((((((((((((((((((....)))))))))))....(((((.((((((((((.....((((..(((.((..((((((.((......(((((((..(((((((((....((((.(((((((((((.....(((((....(((((((.(((((((((((.....)))..................((((((.((......)).)))).)).......)))....))))).))))))).)))))))))))....))))).)))).....))))))))).)))))))......))))))))....))..)))..))))))))))))))...........(((((.....))))).(((.((.((.(((((.......((((((.......))))))))))))).)).)))...)))))....)))))..)))))))).).)))))....((((((.(((......))).))))))............))))) (-182.61) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 409 250 203 73 19 NC_002950:PG0920|5end_glycosyl_transferase,_group_2_family_protein_981025..981255_NEGATIVE 399 369 323 191 134 NC_002950:PG1688|5end_transcription_elongation_factor_GreA_1774686..1774916_NEGATIVE 391 419 372 110 54 NC_002950:PG2145|5end_polysaccharide_deacetylase_2253253..2253483_POSITIVE 385 59 12 93 29 NC_002950:PG0253|5end_hypothetical_protein_284607..284837_POSITIVE 380 237 191 191 136 NC_002950:PG0541|5end_hypothetical_protein_594757..594987_POSITIVE 377 249 201 57 5 NC_002950:PG1888|5end_hypothetical_protein_1985596..1985826_POSITIVE 370 235 195 192 139 NC_002950:PG1697|5end_type_II_restriction_endonuclease,_putative_1781020..1781250_POSITIVE 367 360 311 178 120 NC_002950:PG0307|5end_NADH-ubiquinone_oxidoreductase_342560..342790_POSITIVE 365 380 333 218 168 NC_002950:PG1947|5end_TPR_domain_protein_2032932..2033162_POSITIVE 363 237 197 82 41 NC_002950:PG0724|5end_prolyl_oligopeptidase_family_protein_775229..775459_POSITIVE 363 419 371 57 7 NC_002950:PG0441|5end_hypothetical_protein_478734..478964_POSITIVE 363 449 408 193 155 NC_002950:PG0401|5end_hypothetical_protein_439306..439536_NEGATIVE 362 262 222 56 3 NC_002950:PG1361|5end_dipeptidyl_aminopeptidase_IV,_putative_1442108..1442338_NEGATIVE 362 363 323 50 3 NC_002950:PG0789|5end_hypothetical_protein_841418..841648_NEGATIVE 361 349 301 183 130 NC_002950:PG2154|5end_hypothetical_protein_2260945..2261175_NEGATIVE 361 376 332 168 114 NC_002950:PG1497|5end_DNA-binding_protein,_histone-like_family_1574085..1574315_POSITIVE 361 449 402 131 83 NC_002950:PG0061|5end_yngK_protein_72145..72375_POSITIVE 360 236 197 225 178 NC_002950:PG0156|5end_modification_methylase,_HemK_family_178828..179058_POSITIVE 360 249 207 55 8 NC_002950:PG0140|5end_hypothetical_protein_163858..164088_NEGATIVE 359 347 301 68 15 NC_002950:PG1937|5end_50S_ribosomal_protein_L4_2024136..2024366_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 391 419 372 67 11 NC_002950:PG2144|3end_hypothetical_protein_2253290..2253440_POSITIVE 377 249 201 60 8 NC_002950:PG1887|3end_rhodanese-like_domain_protein_1985679..1985829_POSITIVE 375 359 311 81 27 NC_002950:PG0245|3end_universal_stress_protein_family_279773..279923_NEGATIVE 374 366 323 149 106 NC_002950:PG0599|3end_3,4-dihydroxy-2-butanone_4-phosphate_synthase/GTP_cyclohydrolase_II_657732..657882_POSITIVE 373 364 326 148 108 NC_002950:PG1706|3end_hypothetical_protein_1793849..1793999_NEGATIVE 372 340 296 129 80 NC_002950:PG2040|3end_DNA-binding_protein,_histone-like_family_2138398..2138548_NEGATIVE 367 238 195 55 6 NC_002950:PG1661|3end_hypothetical_protein_1744835..1744985_POSITIVE 365 237 193 128 84 NC_002950:PG1697|3end_type_II_restriction_endonuclease,_putative_1785074..1785224_POSITIVE 363 237 197 128 87 NC_002950:PG0723|3end_hypothetical_protein_775355..775505_POSITIVE 363 419 371 53 3 NC_002950:PG0439|3end_hypothetical_protein_478810..478960_POSITIVE 363 449 408 46 8 NC_002950:PG0403|3end_hypothetical_protein_439453..439603_NEGATIVE 362 363 323 69 22 NC_002950:PG0790|3end_GTP-binding_protein_Obg_841399..841549_NEGATIVE 361 449 402 84 36 NC_002950:PG0060|3end_hypothetical_protein_72178..72328_POSITIVE 360 340 296 129 76 NC_002950:PG1975|3end_hemagglutinin_protein_HagC_2064350..2064500_POSITIVE 360 80 32 128 76 NC_002950:PG0344|3end_purple_acid_phosphatase_378543..378693_NEGATIVE 359 347 301 68 15 NC_002950:PG1938|3end_50S_ribosomal_protein_L3_2024136..2024286_NEGATIVE 357 366 323 146 100 NC_002950:PG1676|3end_phosphoenolpyruvate_carboxykinase_1760489..1760639_NEGATIVE 355 360 311 74 24 NC_002950:PG1397|3end_bifunctional_phosphoribosylaminoimidazolecarboxamide_formyltransferase/IMP_cyclohydrolase_1478829..1478979_NEGATIVE 355 400 351 151 106 NC_002950:PG0363|3end_hypothetical_protein_398377..398527_POSITIVE 353 237 191 73 27 NC_002950:PG1138|3end_pigmentation_and_extracellular_proteinase_regulator_1219594..1219744_NEGATIVE