Origin IGS: ttgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaagagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgtttcccaagccatgaaataccgacttatcgcttactgcaaagttacgatctcttgtacccaaagctattaggtagaacgaagccacaattatccaaatcgatgccacaagtacgcactaaaggaaatcacgctgattacagcatcaccgaaccatatatttgcactgtcgaatatgtcggtgatggtatgtgtcttcgataacatcacgtttcgatgagcgatataggcgagggtgtatgtcaaagaacatgtccttgattctttcactcgaacgtgggaatgtgcgaaggcagatacaatcgaacgattgtattgatggacactaatgaacatctccacagaaagggtatgaatgaaaggatgtagccctactggtacaagccgattattgggcatatcttcgatagcggatgtattcatcttcatcggtaaatatactggcaacgaaatgaacaatgaattgtgtaataataaaaattaagaaagt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 actttcttaatttttattattacacaattcattgttcatttcgttgccagtatatttaccgatgaagatgaatacatccgctatcgaagatatgcccaataatcggcttgtaccagtagggctacatcctttcattcataccctttctgtggagatgttcattagtgtccatcaatacaatcgttcgattgtatctgccttcgcacattcccacgttcgagtgaaagaatcaaggacatgttctttgacatacaccctcgcctatatcgctcatcgaaacgtgatgttatcgaagacacataccatcaccgacatattcgacagtgcaaatatatggttcggtgatgctgtaatcagcgtgatttcctttagtgcgtacttgtggcatcgatttggataattgtggcttcgttctacctaatagctttgggtacaagagatcgtaactttgcagtaagcgataagtcggtatttcatggcttgggaaacatacatttcgagagaataacatatcgttcttcggaacgaatttatttgcgtcagtgcaaatagcaaggtatgtcttgcgttacccgaaattatttcggtaactcaaaacccttgctcttgcagagggcagaacacctccgaagtcggtgttcttggatagaagttttcgttgtcttgattggctattttatcgtgaacgctctctaatggatttcaa Mask Tandem Repeat Region ================================================ ttgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaagagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgtttcccaagccatgaaataccgacttatcgcttactgcaaagttacgatctcttgtacccaaagctattaggtagaacgaagccacaattatccaaatcgatgccacaagtacgcactaaaggaaatcacgctgattacagcatcaccgaaccatatatttgcactgtcgaatatgtcggtgatggtatgtgtcttcgataacatcacgtttcgatgagcgatataggcgagggtgtatgtcaaagaacatgtccttgattctttcactcgaacgtgggaatgtgcgaaggcagatacaatcgaacgattgtattgatggacactaatgaacatctccacagaaagggtatgaatgaaaggatgtagccctactggtacaagccgattattgggcatatcttcgatagcggatgtattcatcttcatcggtaaatatactggcaacgaaatgaacaatgaattgtgtaataataaaaattaagaaagt Find is-nt database================================================ Query_seq: PG0829:PG0831|PG0829:PG0831:hypothetical protein:hypothetical protein:<-->:886837..887542 706 ttgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaagagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgtttcccaagccatgaaataccgacttatcgcttactgcaaagttacgatctcttgtacccaaagctattaggtagaacgaagccacaattatccaaatcgatgccacaagtacgcactaaaggaaatcacgctgattacagcatcaccgaaccatatatttgcactgtcgaatatgtcggtgatggtatgtgtcttcgataacatcacgtttcgatgagcgatataggcgagggtgtatgtcaaagaacatgtccttgattctttcactcgaacgtgggaatgtgcgaaggcagatacaatcgaacgattgtattgatggacactaatgaacatctccacagaaagggtatgaatgaaaggatgtagccctactggtacaagccgattattgggcatatcttcgatagcggatgtattcatcttcatcggtaaatatactggcaacgaaatgaacaatgaattgtgtaataataaaaattaagaaagt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0829:PG0831|PG0829:PG0831:hypothetical protein:hypothetical protein:<-->:886837..887542 706 ttgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaagagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgtttcccaagccatgaaataccgacttatcgcttactgcaaagttacgatctcttgtacccaaagctattaggtagaacgaagccacaattatccaaatcgatgccacaagtacgcactaaaggaaatcacgctgattacagcatcaccgaaccatatatttgcactgtcgaatatgtcggtgatggtatgtgtcttcgataacatcacgtttcgatgagcgatataggcgagggtgtatgtcaaagaacatgtccttgattctttcactcgaacgtgggaatgtgcgaaggcagatacaatcgaacgattgtattgatggacactaatgaacatctccacagaaagggtatgaatgaaaggatgtagccctactggtacaagccgattattgggcatatcttcgatagcggatgtattcatcttcatcggtaaatatactggcaacgaaatgaacaatgaattgtgtaataataaaaattaagaaagt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0829:PG0831|PG0829:PG0831:hypothetical protein:hypothetical protein:<-->:886837..887542 706 ttgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaagagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgtttcccaagccatgaaataccgacttatcgcttactgcaaagttacgatctcttgtacccaaagctattaggtagaacgaagccacaattatccaaatcgatgccacaagtacgcactaaaggaaatcacgctgattacagcatcaccgaaccatatatttgcactgtcgaatatgtcggtgatggtatgtgtcttcgataacatcacgtttcgatgagcgatataggcgagggtgtatgtcaaagaacatgtccttgattctttcactcgaacgtgggaatgtgcgaaggcagatacaatcgaacgattgtattgatggacactaatgaacatctccacagaaagggtatgaatgaaaggatgtagccctactggtacaagccgattattgggcatatcttcgatagcggatgtattcatcttcatcggtaaatatactggcaacgaaatgaacaatgaattgtgtaataataaaaattaagaaagt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaagagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgtttcccaagccatgaaataccgacttatcgcttactgcaaagttacgatctcttgtacccaaagctattaggtagaacgaagccacaattatccaaatcgatgccacaagtacgcactaaaggaaatcacgctgattacagcatcaccgaaccatatatttgcactgtcgaatatgtcggtgatggtatgtgtcttcgataacatcacgtttcgatgagcgatataggcgagggtgtatgtcaaagaacatgtccttgattctttcactcgaacgtgggaatgtgcgaaggcagatacaatcgaacgattgtattgatggacactaatgaacatctccacagaaagggtatgaatgaaaggatgtagccctactggtacaagccgattattgggcatatcttcgatagcggatgtattcatcttcatcggtaaatatactggcaacgaaatgaacaatgaattgtgtaataataaaaattaagaaagt Predict ORF larger than 30AA ================================================ Protein_Len: 54 Strand: + Start: 125 End: 286 ............................................................................................................................ M I S G N A R H T L L F A L T Q I N S F R R T I C Y S L E M Y V S Q A M K Y R L I A Y C K V T I S C T Q S Y .................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 157 End: 417 ............................................................................................................................................................ M C T D A N K F V P K N D M L F S R N V C F P S H E I P T Y R L L Q S Y D L L Y P K L L G R T K P Q L S K S M P Q V R T ................................................................................................................................................................................................................................................................................................. Protein_Len: 30 Strand: + Start: 344 End: 433 ....................................................................................................................................................................................................................................................................................................................................................... M T L I T A S P N H I F A L S N M S V M V C V F D N I T F R ................................................................................................................................................................................................................................................................................. Protein_Len: 45 Strand: + Start: 408 End: 542 ....................................................................................................................................................................................................................................................................................................................................................................................................................... M S S I T S R F D E R Y R R G C M S K N M S L I L S L E R G N V R R Q I Q S N D C I D G H .................................................................................................................................................................... Protein_Len: 30 Strand: + Start: 493 End: 582 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M G M C E G R Y N R T I V L M D T N E H L H R K G M N E R M ............................................................................................................................ Protein_Len: 42 Strand: + Start: 572 End: 697 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M K G C S P T G T S R L L G I S S I A D V F I F I G K Y T G N E M N N E L C N N K N ......... Protein_Len: 30 Strand: - Start: 608 End: 697 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Q A Y R R Y R I Y E D E D T F I S A V F H V I F Q T I I F M ......... Protein_Len: 32 Strand: - Start: 486 End: 581 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E F T P I H S P L Y L R V I T N I S V L S C R W L F P I F S L M ............................................................................................................................. Protein_Len: 56 Strand: - Start: 365 End: 532 ............................................................................................................................................................................................................................................................................................................................................................................ R V M Y K C Q R I H R H H Y T D E I V D R K S S R Y L R P H I D F F M D K I R E S S R P F T R L C I C D F S Q M .............................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 310 End: 675 ..................................................................................................................................................................................................................................................................................................................... S H A F A S V I S R N Y Q H V S I F M E V S L T H I F P H L G V P V L R N N P M D E I A S T N M K M P L Y V P L S I F M ............................... Protein_Len: 56 Strand: - Start: 158 End: 325 ............................................................................................................................................................. K C Q R L Y I R E S S R Y T I R E F H I N G L G H F V S K D S V A F N R D R T G L A I L Y F S A V I I W I S A M ............................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 39 Strand: - Start: 120 End: 236 ....................................................................................................................... R F L K P Y R L V Y R A I Q V S A F L N T G F F S I N N E R F T H K G L W S M ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 45 Strand: - Start: 61 End: 195 ............................................................ G L V G V E S T N Q G R C S C P N Q T V S I I E P L A L C V K S N A S V C I F E N R L V M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 45 Strand: - Start: 14 End: 148 ............. L A N V I F Y G I L V V F V E I W S C R S R L H E A R Q L L L P K S N G F Y N R T V C S M .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. ttgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaagagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgtttcccaagccatgaaataccgacttatcgcttactgcaaagttacgatctcttgtacccaaagctattaggtagaacgaagccacaattatccaaatcgatgccacaagtacgcactaaaggaaatcacgctgattacagcatcaccgaaccatatatttgcactgtcgaatatgtcggtgatggtatgtgtcttcgataacatcacgtttcgatgagcgatataggcgagggtgtatgtcaaagaacatgtccttgattctttcactcgaacgtgggaatgtgcgaaggcagatacaatcgaacgattgtattgatggacactaatgaacatctccacagaaagggtatgaatgaaaggatgtagccctactggtacaagccgattattgggcatatcttcgatagcggatgtattcatcttcatcggtaaatatactggcaacgaaatgaacaatgaattgtgtaataataaaaattaagaaagt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaagagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgtttcccaagccatgaaataccgacttatcgcttactgcaaagttacgatctcttgtacccaaagctattaggtagaacgaagccacaattatccaaatcgatgccacaagtacgcactaaaggaaatcacgctgattacagcatcaccgaaccatatatttgcactgtcgaatatgtcggtgatggtatgtgtcttcgataacatcacgtttcgatgagcgatataggcgagggtgtatgtcaaagaacatgtccttgattctttcactcgaacgtgggaatgtgcgaaggcagatacaatcgaacgattgtattgatggacactaatgaacatctccacagaaagggtatgaatgaaaggatgtagccctactggtacaagccgattattgggcatatcttcgatagcggatgtattcatcttcatcggtaaatatactggcaacgaaatgaacaatgaattgtgtaataataaaaattaagaaagt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 53 HP_score: -4.7 Tail_Score: -3.38046 Start: 140 End: 155 Full_Region: ATAATTTCGGGTAAC GCAAG ACATAC CTTGC TATTTGCACTGACGC ...........................................................................................................................................GCAAGACATACCTTGC....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 70 HP_score: -5.4 Tail_Score: -4.46514 Start: 178 End: 188 Full_Region: aataacatatcgttc ttcg gaa cgaa tttatttgcgtcagt .................................................................................................................................................................................ttcggaacgaa...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: PG0829:PG0831|PG0829:PG0831:hypothetical protein:hypothetical protein:<-->:886837..887542 706 ttgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaagagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgtttcccaagccatgaaataccgacttatcgcttactgcaaagttacgatctcttgtacccaaagctattaggtagaacgaagccacaattatccaaatcgatgccacaagtacgcactaaaggaaatcacgctgattacagcatcaccgaaccatatatttgcactgtcgaatatgtcggtgatggtatgtgtcttcgataacatcacgtttcgatgagcgatataggcgagggtgtatgtcaaagaacatgtccttgattctttcactcgaacgtgggaatgtgcgaaggcagatacaatcgaacgattgtattgatggacactaatgaacatctccacagaaagggtatgaatgaaaggatgtagccctactggtacaagccgattattgggcatatcttcgatagcggatgtattcatcttcatcggtaaatatactggcaacgaaatgaacaatgaattgtgtaataataaaaattaagaaagt Intra-Species Hit: Count: 2 Min: 1 Max: 706 Len: 706 Subject: NC_002950_PG0829_PG0831|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[886837,887542]|706 HSP 1 e-value: 0.0 bit: 1400.0 Len: 706 Query Start:1 Query End:706 Subject Strand: POSITIVE Subject Start: 1 Subject End: 706 ttgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaagagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgtttcccaagccatgaaataccgacttatcgcttactgcaaagttacgatctcttgtacccaaagctattaggtagaacgaagccacaattatccaaatcgatgccacaagtacgcactaaaggaaatcacgctgattacagcatcaccgaaccatatatttgcactgtcgaatatgtcggtgatggtatgtgtcttcgataacatcacgtttcgatgagcgatataggcgagggtgtatgtcaaagaacatgtccttgattctttcactcgaacgtgggaatgtgcgaaggcagatacaatcgaacgattgtattgatggacactaatgaacatctccacagaaagggtatgaatgaaaggatgtagccctactggtacaagccgattattgggcatatcttcgatagcggatgtattcatcttcatcggtaaatatactggcaacgaaatgaacaatgaattgtgtaataataaaaattaagaaagt ttgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaagagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgtttcccaagccatgaaataccgacttatcgcttactgcaaagttacgatctcttgtacccaaagctattaggtagaacgaagccacaattatccaaatcgatgccacaagtacgcactaaaggaaatcacgctgattacagcatcaccgaaccatatatttgcactgtcgaatatgtcggtgatggtatgtgtcttcgataacatcacgtttcgatgagcgatataggcgagggtgtatgtcaaagaacatgtccttgattctttcactcgaacgtgggaatgtgcgaaggcagatacaatcgaacgattgtattgatggacactaatgaacatctccacagaaagggtatgaatgaaaggatgtagccctactggtacaagccgattattgggcatatcttcgatagcggatgtattcatcttcatcggtaaatatactggcaacgaaatgaacaatgaattgtgtaataataaaaattaagaaagt Subject: NC_002950_PG1442_PG1444|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[1528121,1528885]|765 HSP 1 e-value: 1.0E-140 bit: 500.0 Len: 264 Query Start:1 Query End:264 Subject Strand: NEGATIVE Subject Start: 442 Subject End: 705 ttgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaagagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgtttcccaagccatgaaataccgacttatcgcttactgcaaagttac.......................................................................................................................................................................................................................................................................................................................................................................................................................................................... ttgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaggagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgttttcaaagccatgaaataccgacttatcgcttactgcaaagttac.......................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-136 bit: 486.0 Len: 401 Query Start:295 Query End:695 Subject Strand: NEGATIVE Subject Start: 11 Subject End: 411 ......................................................................................................................................................................................................................................................................................................acgaagccacaattatccaaatcgatgccacaagtacgcactaaaggaaatcacgctgattacagcatcaccgaaccatatatttgcactgtcgaatatgtcggtgatggtatgtgtcttcgataacatcacgtttcgatgagcgatataggcgagggtgtatgtcaaagaacatgtccttgattctttcactcgaacgtgggaatgtgcgaaggcagatacaatcgaacgattgtattgatggacactaatgaacatctccacagaaagggtatgaatgaaaggatgtagccctactggtacaagccgattattgggcatatcttcgatagcggatgtattcatcttcatcggtaaatatactggcaacgaaatgaacaatgaattgtgtaataataaaa........... ......................................................................................................................................................................................................................................................................................................acgaagccacaactatccaaatcgaagccacaagtacgcactaaagtaaatcacgctgattacagcatcaccgagccatatatttgcaccgtcgaatatgtcggtggaggaatgtgtcttcgatgatatcacgattcgatgagcgatataagcgagggggtatatcgaagaacacgtccttgattctttcacttgaacgtggaaatgtgtgaaagcagatacaatcgaacgattgtattgatggacatcaatgaacatctccacaaaaagggtatgaacgaaaggatgtagccctgctggtacaaccccattatcgggcatatcttagatagtggatgtattcatctttgcaggtaaatataccggcaaagaaatgaacagtgaattgtataacaataaaa........... Inter-species Hit: Count: 2 Min: 1 Max: 695 Len: 695 Subject: pint_PI0397_PI0398|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[362960,363517]|558 HSP 1 e-value: 1.0E-142 bit: 507.0 Len: 264 Query Start:1 Query End:264 Subject Strand: POSITIVE Subject Start: 61 Subject End: 324 ttgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaagagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgtttcccaagccatgaaataccgacttatcgcttactgcaaagttac.......................................................................................................................................................................................................................................................................................................................................................................................................................................................... ttgaaatccattagagagcgttcacgataaaatagccaatcaagacaacgaaaacttctatccaagaacaccgacttcggaggtgttctgccctctgcaagagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaattcgttccgaagaacgatatgttattctctcgaaatgtatgttttcaaagccatgaaataccgacttatcgcttactgcaaagttac.......................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 4.0E-72 bit: 274.0 Len: 202 Query Start:295 Query End:496 Subject Strand: POSITIVE Subject Start: 355 Subject End: 556 ......................................................................................................................................................................................................................................................................................................acgaagccacaattatccaaatcgatgccacaagtacgcactaaaggaaatcacgctgattacagcatcaccgaaccatatatttgcactgtcgaatatgtcggtgatggtatgtgtcttcgataacatcacgtttcgatgagcgatataggcgagggtgtatgtcaaagaacatgtccttgattctttcactcgaacgtgg.................................................................................................................................................................................................................. ......................................................................................................................................................................................................................................................................................................acgaagccacaactatccaaatcgaagccacaagtacgcactaaagtaaatcacgctgattacagcatcaccgagccatatatttgcaccgtcgaatatgtcggtggaggaatgtgtcttcgatgatatcacgattcgatgagcgatataagcgagggggtatatcaaagaacacgtccttgattctttcacttgaacgtgg.................................................................................................................................................................................................................. Subject: pint_PI0398_PI0399|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[363602,363724]|123 HSP 1 e-value: 2.0E-21 bit: 105.0 Len: 113 Query Start:583 Query End:695 Subject Strand: POSITIVE Subject Start: 1 Subject End: 113 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tagccctactggtacaagccgattattgggcatatcttcgatagcggatgtattcatcttcatcggtaaatatactggcaacgaaatgaacaatgaattgtgtaataataaaa........... ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tagccctgctggtacaaccccattatcgggcatatcttagatagtggatgtattcatctttgcaggtaaatataccggcaaagaaatgaacagtgaattgtataacaataaaa........... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGAAAUCCAUUAGAGAGCGUUCACGAUAAAAUAGCCAAUCAAGACAACGAAAACUUCUAUCCAAGAACACCGACUUCGGAGGUGUUCUGCCCUCUGCAAGAGCAAGGGUUUUGAGUUACCGAAAUAAUUUCGGGUAACGCAAGACAUACCUUGCUAUUUGCACUGACGCAAAUAAAUUCGUUCCGAAGAACGAUAUGUUAUUCUCUCGAAAUGUAUGUUUCCCAAGCCAUGAAAUACCGACUUAUCGCUUACUGCAAAGUUACGAUCUCUUGUACCCAAAGCUAUUAGGUAGAACGAAGCCACAAUUAUCCAAAUCGAUGCCACAAGUACGCACUAAAGGAAAUCACGCUGAUUACAGCAUCACCGAACCAUAUAUUUGCACUGUCGAAUAUGUCGGUGAUGGUAUGUGUCUUCGAUAACAUCACGUUUCGAUGAGCGAUAUAGGCGAGGGUGUAUGUCAAAGAACAUGUCCUUGAUUCUUUCACUCGAACGUGGGAAUGUGCGAAGGCAGAUACAAUCGAACGAUUGUAUUGAUGGACACUAAUGAACAUCUCCACAGAAAGGGUAUGAAUGAAAGGAUGUAGCCCUACUGGUACAAGCCGAUUAUUGGGCAUAUCUUCGAUAGCGGAUGUAUUCAUCUUCAUCGGUAAAUAUACUGGCAACGAAAUGAACAAUGAAUUGUGUAAUAAUAAAA ............((((.((......(((..........))).......................((((((((..(....).)))))))))).))))((((((((((((((((((.(((((((((.....))).)))))).))))))...))))))).)))))....((((((....(((((((.((((((..((((((((...(((((((((((((((((...............((((.....((.((((..(((((((.......)).)))))....)))).))..))))...(((((.((((.....(((....(((.((...(((....)))...))..)))..((((....)))).(((((((....((((((((......))))))))))))))))))..)))).)))))..))))).))))))))).))).((((((.((....)).))))))....))))))))....(((((((((.(((.((((((..((((((....)).(((((((((....)))))))))...............))))..)))).......)).))).)))))))))...((((...((((....))))......))))...))))))..)))))))...(((((.(((.((((((....))))))....)))))))).......)))))).......... (-190.71) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUAUUAUUACACAAUUCAUUGUUCAUUUCGUUGCCAGUAUAUUUACCGAUGAAGAUGAAUACAUCCGCUAUCGAAGAUAUGCCCAAUAAUCGGCUUGUACCAGUAGGGCUACAUCCUUUCAUUCAUACCCUUUCUGUGGAGAUGUUCAUUAGUGUCCAUCAAUACAAUCGUUCGAUUGUAUCUGCCUUCGCACAUUCCCACGUUCGAGUGAAAGAAUCAAGGACAUGUUCUUUGACAUACACCCUCGCCUAUAUCGCUCAUCGAAACGUGAUGUUAUCGAAGACACAUACCAUCACCGACAUAUUCGACAGUGCAAAUAUAUGGUUCGGUGAUGCUGUAAUCAGCGUGAUUUCCUUUAGUGCGUACUUGUGGCAUCGAUUUGGAUAAUUGUGGCUUCGUUCUACCUAAUAGCUUUGGGUACAAGAGAUCGUAACUUUGCAGUAAGCGAUAAGUCGGUAUUUCAUGGCUUGGGAAACAUACAUUUCGAGAGAAUAACAUAUCGUUCUUCGGAACGAAUUUAUUUGCGUCAGUGCAAAUAGCAAGGUAUGUCUUGCGUUACCCGAAAUUAUUUCGGUAACUCAAAACCCUUGCUCUUGCAGAGGGCAGAACACCUCCGAAGUCGGUGUUCUUGGAUAGAAGUUUUCGUUGUCUUGAUUGGCUAUUUUAUCGUGAACGCUCUCUAAUGGAUUUCAA ...........((((((....((((((..(((.((((.((((((((..(((((..((((....))))...))))).))))))))..........((..((((((.((.(((......(((((((((..((.......((((..((((......(((.......((((((((....)))))))).......)))))))..))))))..)))))))))..(((((((.....)))))))...........))).(((((((..........)))))))....((((.(((.....(((((((((((((..............)))))..))))))))((((....))))))).))))...)).)).))))..)))))).)))..)))))))))))).........(((((((.....)))))))...((((((........((.((..((((((((..(((...(((.(((...((((((......(((....))).......((((((....)))))).(((((((..(((..(((((..(((((((...((.(((.((((((.(((.....))))))))).))).))))))))).)))))...))).(((((((.........)))))))..))))))).))))))...))).)))...)))..))))))))..)))).........)))))).. (-195.42) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1060 110 61 201 152 NC_002950:PG0829|5end_hypothetical_protein_886746..886976_NEGATIVE 889 80 31 231 182 NC_002950:PG1444|5end_hypothetical_protein_1528746..1528976_POSITIVE 418 110 62 209 150 NC_002950:PG2112|5end_hypothetical_protein_2221471..2221701_POSITIVE 385 122 81 164 124 NC_002950:PG2076|5end_hypothetical_protein_2176445..2176675_POSITIVE 385 122 81 164 124 NC_002950:PG1267|5end_hypothetical_protein_1346966..1347196_POSITIVE 377 116 71 73 31 NC_002950:PG2032|5end_primosomal_protein_N'_2133381..2133611_NEGATIVE 376 122 81 164 124 NC_002950:PG1672|5end_hypothetical_protein_1757637..1757867_POSITIVE 376 122 81 164 124 NC_002950:PG0100|5end_hypothetical_protein_119219..119449_NEGATIVE 372 108 62 180 124 NC_002950:PG0607|5end_hypothetical_protein_662552..662782_POSITIVE 371 629 581 230 179 NC_002950:PG0758|5end_peptidyl-dipeptidase_Dcp_808554..808784_NEGATIVE 370 640 594 199 142 NC_002950:PG1381|5end_ABC_transporter,_permease_protein_1460622..1460852_POSITIVE 370 453 413 207 164 NC_002950:PG0918|5end_hypothetical_protein_978818..979048_NEGATIVE 356 646 602 167 112 NC_002950:PG0002|5end_hexapeptide_transferase_family_protein_1295..1525_POSITIVE 353 123 82 123 64 NC_002950:PG1655|5end_hypothetical_protein_1737095..1737325_POSITIVE 352 119 74 56 10 NC_002950:PG1088|5end_acetyltransferase,_GNAT_family_1155426..1155656_NEGATIVE 346 630 586 200 149 NC_002950:PG1125|5end_hypothetical_protein_1201727..1201957_NEGATIVE 345 647 601 231 184 NC_002950:PG0447|5end_hypothetical_protein_487278..487508_POSITIVE 341 110 61 203 145 NC_002950:PG0093|5end_HlyD_family_secretion_protein_109638..109868_NEGATIVE 340 99 73 38 8 NC_002950:PG1988|5end_hypothetical_protein_2078867..2079097_NEGATIVE 338 110 62 219 153 NC_002950:PG1616|5end_hypothetical_protein_1697250..1697480_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 365 103 66 68 24 NC_002950:PG1949|3end_malate_dehydrogenase_2038184..2038334_NEGATIVE 353 123 82 109 50 NC_002950:PG1654|3end_D-alanyl-D-alanine_dipeptidase_1737161..1737311_POSITIVE 344 120 71 85 34 NC_002950:PG1330|3end_large_conductance_mechanosensitive_channel_protein_1410165..1410315_POSITIVE 342 116 77 39 1 NC_002950:PG2033|3end_glutamate_synthase,_small_subunit_2133415..2133565_NEGATIVE 341 513 473 143 98 NC_002950:PG1221|3end_oxidoreductase,_short_chain_dehydrogenase/reductase_family_1298934..1299084_POSITIVE 338 628 581 151 101 NC_002950:PG0759|3end_TPR_domain_protein_808632..808782_NEGATIVE 331 538 493 97 55 NC_002950:PG1595|3end_ribulose-phosphate_3-epimerase_1673594..1673744_NEGATIVE 330 630 581 146 102 NC_002950:PG1824|3end_phosphopyruvate_hydratase_1918153..1918303_NEGATIVE 330 629 584 147 109 NC_002950:PG1541|3end_2-amino-4-hydroxy-6-_hydroxymethyldihydropteridine_pyrophosphokinase_1620855..1621005_POSITIVE 329 256 214 78 30 NC_002950:PG0799|3end_hypothetical_protein_854599..854749_POSITIVE 328 108 71 149 113 NC_002950:PG1677|3end_phosphoglycerate_kinase_1763678..1763828_POSITIVE 325 118 72 99 48 NC_002950:PG0328|3end_imidazolonepropionase_362155..362305_NEGATIVE 324 100 55 146 89 NC_002950:PG0257|3end_hypothetical_protein_291559..291709_POSITIVE 320 105 61 143 95 NC_002950:PG0094|3end_outer_membrane_efflux_protein,_putative_109688..109838_NEGATIVE 319 627 584 85 30 NC_002950:PG1835|3end_lipoprotein,_putative_1928520..1928670_POSITIVE 319 268 222 64 22 NC_002950:PG1474|3end_conjugative_transposon_protein_TraO_1550238..1550388_NEGATIVE 318 108 72 144 105 NC_002950:PG1487|3end_hypothetical_protein_1561799..1561949_POSITIVE 317 111 71 92 39 NC_002950:PG1494|3end_hypothetical_protein_1571234..1571384_POSITIVE 313 115 76 66 15 NC_002950:PG0461|3end_ISPg7,_transposase_503439..503589_NEGATIVE 311 119 71 93 31 NC_002950:PG0240|3end_hydrolase,_haloacid_dehalogenase-like_family_276395..276545_NEGATIVE