Origin IGS: acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaacctttgcaaaggggagattcaatggacggagaggggaaaaaatgtatcagaactgctctttgtcattgacacggagcttgaattactgaaaatgcgaatgcagggacttcttcctgcattgccagtcagacctgctaagaaactctgttggacaggcaaagcaacagatttagttgaactcctttatgctctcgacacttgcaacagtatcaacaacggagaaataggtgtagaggaactggcagacgctctctcggagatcttcggagtggcgataaagaacagctacaacgtctatatgaacatgaaacgtcgcaaagatgatagccgcacctattttcttgatgaactccgtgagaaacttaataagcgtatggtggaaagcgatctgaaaggcggcaagttcaaaaagcgataatcgcattggttaatcaggcttcctcaaataaaagggaagtctgatttttacatccatgcaaggagcaatatgatataatatgt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 acatattatatcatattgctccttgcatggatgtaaaaatcagacttcccttttatttgaggaagcctgattaaccaatgcgattatcgctttttgaacttgccgcctttcagatcgctttccaccatacgcttattaagtttctcacggagttcatcaagaaaataggtgcggctatcatctttgcgacgtttcatgttcatatagacgttgtagctgttctttatcgccactccgaagatctccgagagagcgtctgccagttcctctacacctatttctccgttgttgatactgttgcaagtgtcgagagcataaaggagttcaactaaatctgttgctttgcctgtccaacagagtttcttagcaggtctgactggcaatgcaggaagaagtccctgcattcgcattttcagtaattcaagctccgtgtcaatgacaaagagcagttctgatacattttttcccctctccgtccattgaatctcccctttgcaaaggttgcaggcaaaagcgtgtataacggagggtccgaaacaatgagactaaatctctttctgcatcacatagggcaataatcttcagaataaagttgtctgacgctttttgtatctgtccttttgtagagcataaagagattattgtcttaaagtcttcagtttgagtaagtcttagcataatcatatcttttatgt Mask Tandem Repeat Region ================================================ acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaacctttgcaaaggggagattcaatggacggagaggggaaaaaatgtatcagaactgctctttgtcattgacacggagcttgaattactgaaaatgcgaatgcagggacttcttcctgcattgccagtcagacctgctaagaaactctgttggacaggcaaagcaacagatttagttgaactcctttatgctctcgacacttgcaacagtatcaacaacggagaaataggtgtagaggaactggcagacgctctctcggagatcttcggagtggcgataaagaacagctacaacgtctatatgaacatgaaacgtcgcaaagatgatagccgcacctattttcttgatgaactccgtgagaaacttaataagcgtatggtggaaagcgatctgaaaggcggcaagttcaaaaagcgataatcgcattggttaatcaggcttcctcaaataaaagggaagtctgatttttacatccatgcaaggagcaatatgatataatatgt Find is-nt database================================================ Query_seq: PG0827:PG0829|PG0827:PG0829:MATE efflux family protein:hypothetical protein:-><-:885665..886359 695 acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaacctttgcaaaggggagattcaatggacggagaggggaaaaaatgtatcagaactgctctttgtcattgacacggagcttgaattactgaaaatgcgaatgcagggacttcttcctgcattgccagtcagacctgctaagaaactctgttggacaggcaaagcaacagatttagttgaactcctttatgctctcgacacttgcaacagtatcaacaacggagaaataggtgtagaggaactggcagacgctctctcggagatcttcggagtggcgataaagaacagctacaacgtctatatgaacatgaaacgtcgcaaagatgatagccgcacctattttcttgatgaactccgtgagaaacttaataagcgtatggtggaaagcgatctgaaaggcggcaagttcaaaaagcgataatcgcattggttaatcaggcttcctcaaataaaagggaagtctgatttttacatccatgcaaggagcaatatgatataatatgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0827:PG0829|PG0827:PG0829:MATE efflux family protein:hypothetical protein:-><-:885665..886359 695 acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaacctttgcaaaggggagattcaatggacggagaggggaaaaaatgtatcagaactgctctttgtcattgacacggagcttgaattactgaaaatgcgaatgcagggacttcttcctgcattgccagtcagacctgctaagaaactctgttggacaggcaaagcaacagatttagttgaactcctttatgctctcgacacttgcaacagtatcaacaacggagaaataggtgtagaggaactggcagacgctctctcggagatcttcggagtggcgataaagaacagctacaacgtctatatgaacatgaaacgtcgcaaagatgatagccgcacctattttcttgatgaactccgtgagaaacttaataagcgtatggtggaaagcgatctgaaaggcggcaagttcaaaaagcgataatcgcattggttaatcaggcttcctcaaataaaagggaagtctgatttttacatccatgcaaggagcaatatgatataatatgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0827:PG0829|PG0827:PG0829:MATE efflux family protein:hypothetical protein:-><-:885665..886359 695 acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaacctttgcaaaggggagattcaatggacggagaggggaaaaaatgtatcagaactgctctttgtcattgacacggagcttgaattactgaaaatgcgaatgcagggacttcttcctgcattgccagtcagacctgctaagaaactctgttggacaggcaaagcaacagatttagttgaactcctttatgctctcgacacttgcaacagtatcaacaacggagaaataggtgtagaggaactggcagacgctctctcggagatcttcggagtggcgataaagaacagctacaacgtctatatgaacatgaaacgtcgcaaagatgatagccgcacctattttcttgatgaactccgtgagaaacttaataagcgtatggtggaaagcgatctgaaaggcggcaagttcaaaaagcgataatcgcattggttaatcaggcttcctcaaataaaagggaagtctgatttttacatccatgcaaggagcaatatgatataatatgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 11 Min: 241 Max: 588 Len: 348 Subject: UniRef90_Q45784 Cluster: RteC protein; n=3; Bacteroides|Rep: RteC protein - Bacteroides thetaiotaomicron HSP 1 e-value: 2.0E-18 bit: 94.7 Len: 246 Query Start:337 Query End:582 Subject Strand: null Subject Start: 129 Subject End: 210 ................................................................................................................................................................................................................................................................................................................................................ L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D ................................................................................................................. ................................................................................................................................................................................................................................................................................................................................................ L Q W T G N A I D L V E L I Y G I D E M G C I N N G N M P L K Q L A P L L Y K I F G V E S K D C Y R F Y T D I K R R K N E S R T Y F L D R M Q E K L N E R M L R D D ................................................................................................................. Subject: UniRef90_Q8A5D2 Cluster: Tetracycline resistance element mobilization regulatory protein rteC; n=1; Bacteroides thetaiotaomicron|Rep: Tetracycline resistance element mobilization regulatory protein rteC - Bacteroides thetaiotaomicron HSP 1 e-value: 6.0E-12 bit: 73.6 Len: 246 Query Start:337 Query End:582 Subject Strand: null Subject Start: 117 Subject End: 198 ................................................................................................................................................................................................................................................................................................................................................ L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D ................................................................................................................. ................................................................................................................................................................................................................................................................................................................................................ V C W T A E V I C L M E L L Y A C H E L K M F N G G E V T L G R V V E Y V C H V L G V N V K N A S S Y Y A R M R R R K A G D R T Y F I D R L R E V L L K R M E D D D ................................................................................................................. Subject: UniRef90_A3UA55 Cluster: Putative regulatory protein; n=1; Croceibacter atlanticus HTCC2559|Rep: Putative regulatory protein - Croceibacter atlanticus HTCC2559 HSP 1 e-value: 2.0E-10 bit: 68.2 Len: 315 Query Start:274 Query End:588 Subject Strand: null Subject Start: 178 Subject End: 284 ................................................................................................................................................................................................................................................................................. E L L K M R M Q G L - L P A L P V R P A - - K K L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D L K ........................................................................................................... ................................................................................................................................................................................................................................................................................. E L D R L R K K E L N L S I E P D S P A I L N N L S W T A S K T D L V E I I Y A L Q A S G A I R N G Q A E I N K M A L V C E K L F D L E L G N F Y R T Y L E I K E R K N D - R T R F L T K L K R D L E Q K M K N D D E K ........................................................................................................... Subject: UniRef90_Q64WJ7 Cluster: Putative regulatory protein RteC homolog; n=1; Bacteroides fragilis|Rep: Putative regulatory protein RteC homolog - Bacteroides fragilis HSP 1 e-value: 7.0E-10 bit: 66.6 Len: 252 Query Start:331 Query End:582 Subject Strand: null Subject Start: 218 Subject End: 300 .......................................................................................................................................................................................................................................................................................................................................... K K L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D ................................................................................................................. .......................................................................................................................................................................................................................................................................................................................................... K V L R W T G T K R A L V E L I Y A L D A C G C L N K G T V D I K E I V A Y F E Y V F D I D L G D F Y H T Y M E L K A K T K D - R T G F L S T L K D R L L M R M R E Q D ................................................................................................................. Subject: UniRef90_A3XRA5 Cluster: Putative regulatory protein RteC-like protein; n=1; Leeuwenhoekiella blandensis MED217|Rep: Putative regulatory protein RteC-like protein - Leeuwenhoekiella blandensis MED217 HSP 1 e-value: 7.0E-10 bit: 66.6 Len: 252 Query Start:331 Query End:582 Subject Strand: null Subject Start: 195 Subject End: 277 .......................................................................................................................................................................................................................................................................................................................................... K K L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D ................................................................................................................. .......................................................................................................................................................................................................................................................................................................................................... K V L V W T G S K T A L V E L I Y A L Y S D Q V I N H G A L D L K Q L T S G F E D F F N I K L Q H P Y K T Y T E L K E R K - G S R T K F L N E L I L K L E Q K M L A E D ................................................................................................................. Subject: UniRef90_Q5LFW1 Cluster: Hypothetical protein; n=1; Bacteroides fragilis NCTC 9343|Rep: Hypothetical protein - Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) HSP 1 e-value: 1.0E-8 bit: 62.8 Len: 246 Query Start:343 Query End:588 Subject Strand: null Subject Start: 202 Subject End: 281 ...................................................................................................................................................................................................................................................................................................................................................... W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D L K ........................................................................................................... ...................................................................................................................................................................................................................................................................................................................................................... F T G K K A Y L I E L G Y S L V S S G D I N N G N V E I K E M M N F L S T I F H I D L G D Y Y A S Y I A M K E R K D - - R T A Y L H H L I D S L I K R M N E D D M K ........................................................................................................... Subject: UniRef90_Q8A7U7 Cluster: Tetracycline resistance element mobilization regulatory protein rteC; n=1; Bacteroides thetaiotaomicron|Rep: Tetracycline resistance element mobilization regulatory protein rteC - Bacteroides thetaiotaomicron HSP 1 e-value: 1.0E-8 bit: 62.4 Len: 342 Query Start:241 Query End:582 Subject Strand: null Subject Start: 168 Subject End: 278 ................................................................................................................................................................................................................................................ S E L L F V I D T E L E L L K M R M Q G L L P A L P V R P A K K L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D ................................................................................................................. ................................................................................................................................................................................................................................................ S H L I E Y V S D E I D S L S D K L H - - L K F T S I V D S K L L Q W T D S K V A L V E F I Y A I Y A G K C F N N G N T S L K D I A F C C E I L F N I E I G D F Y R I F L E I R N R K - K S R T Q F L D K L K D K I Q K M M E E L D ................................................................................................................. Subject: UniRef90_A3UA76 Cluster: Putative regulatory protein; n=1; Croceibacter atlanticus HTCC2559|Rep: Putative regulatory protein - Croceibacter atlanticus HTCC2559 HSP 1 e-value: 1.0E-7 bit: 59.3 Len: 240 Query Start:343 Query End:582 Subject Strand: null Subject Start: 204 Subject End: 282 ...................................................................................................................................................................................................................................................................................................................................................... W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D ................................................................................................................. ...................................................................................................................................................................................................................................................................................................................................................... W T S N K I D L V E L I Y A L Q T S R A I Q N G K I S I T T L A N V F G T V F N T D L S N I H R T Y V E L K S R K K D - R C K F M K Y L M V A L E N R V I T E E ................................................................................................................. Subject: UniRef90_Q1XQ17 Cluster: Putative regulatory protein RteC-like; n=1; Flavobacterium johnsoniae UW101|Rep: Putative regulatory protein RteC-like - Flavobacterium johnsoniae UW101 HSP 1 e-value: 1.0E-7 bit: 58.9 Len: 252 Query Start:337 Query End:588 Subject Strand: null Subject Start: 198 Subject End: 280 ................................................................................................................................................................................................................................................................................................................................................ L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D L K ........................................................................................................... ................................................................................................................................................................................................................................................................................................................................................ L V W T A S K T A L I E L I Y A L H T Q G V F G N G T I D I K V I A T Y F E Q T F N V D L G D F Y H T F L E L R N R K - T N R T K F I D T L K E G L L R R M D D Q E E K ........................................................................................................... Subject: UniRef90_A0M154 Cluster: Regulatory protein RteC; n=1; Gramella forsetii KT0803|Rep: Regulatory protein RteC - Gramella forsetii (strain KT0803) HSP 1 e-value: 1.0E-6 bit: 55.8 Len: 309 Query Start:274 Query End:582 Subject Strand: null Subject Start: 191 Subject End: 292 ................................................................................................................................................................................................................................................................................. E L L K M R M Q G L L P A L P V R P A K K L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D ................................................................................................................. ................................................................................................................................................................................................................................................................................. E A M E T L R P G K Q S G L M D K K H K V M L W S G S K T S L V E L I Y A L Y A N G D L N H G T V D I S T I T S S F E D F F N I K L E N I Y K T Y S E I K S R K - G T R A K Y L H N L I L R L E A K M N Q D D ................................................................................................................. Subject: UniRef90_A3XIY9 Cluster: Putative regulatory protein RteC-like protein; n=1; Leeuwenhoekiella blandensis MED217|Rep: Putative regulatory protein RteC-like protein - Leeuwenhoekiella blandensis MED217 HSP 1 e-value: 1.0E-6 bit: 55.8 Len: 264 Query Start:319 Query End:582 Subject Strand: null Subject Start: 191 Subject End: 277 .............................................................................................................................................................................................................................................................................................................................. V R P A K K L C W T G K A T D L V E L L Y A L D T C N S I N N G E I G V E E L A D A L S E I F G V A I K N S Y N V Y M N M K R R K D D S R T Y F L D E L R E K L N K R M V E S D ................................................................................................................. .............................................................................................................................................................................................................................................................................................................................. I K P M E K I D W P F S N T D Y V E L L Y A L C S K G L G K R N N Q G I M Q V S K K L E Q I F N I E P K D I Y K T F Q D I K N R K - K S R T L F L D D L S T S L L S E M N R S E ................................................................................................................. acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaacctttgcaaaggggagattcaatggacggagaggggaaaaaatgtannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggcggcaagttcaaaaagcgataatcgcattggttaatcaggcttcctcaaataaaagggaagtctgatttttacatccatgcaaggagcaatatgatataatatgt Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 3 End: 260 .. M K D M I M L R L T Q T E D F K T I I S L C S T K G Q I Q K A S D N F I L K I I A L C D A E R D L V S L F R T L R Y T R ................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 42 Strand: + Start: 155 End: 280 .......................................................................................................................................................... M V S D P P L Y T L L P A T F A K G R F N G R R G E K M Y Q N C S L S L T R S L N Y ............................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 41 Strand: + Start: 393 End: 515 ........................................................................................................................................................................................................................................................................................................................................................................................................ M Q Q Y Q Q R R N R C R G T G R R S L G D L R S G D K E Q L Q R L Y E H E T S Q R .................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 175 End: 609 .............................................................................................................................................................................. M H A F A C N L C K G E I Q W T E R G K N V S E L L F V I D T E L E L L K M R M Q G L L P A L P V R P A K K L C W T G K ...................................................................................... Protein_Len: 33 Strand: - Start: 531 End: 629 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. K R S S S R S F S L L R I T S L S R F P P L N L F R Y D C Q N I M .................................................................. Protein_Len: 33 Strand: - Start: 381 End: 479 ............................................................................................................................................................................................................................................................................................................................................................................................ A R S V Q L L I L L P S I P T S S S A S A R E S I K P T A I F F M ........................................................................................................................................................................................................................ Protein_Len: 60 Strand: - Start: 367 End: 618 .............................................................................................................................................................................................................................................................................................................................................................................. V S E R L D E S H R Y L V A V V D I H V H F T A F I I A A G I K K I F E T L F K I L T H H F A I Q F A A L E F L S L R M ............................................................................. Protein_Len: 46 Strand: - Start: 216 End: 353 ....................................................................................................................................................................................................................... H V S L P F F T D S S S K T M S V S S S N S F I R I C P S R G A N G T L G A L F S Q Q V P M ...................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 39 Strand: - Start: 121 End: 237 ........................................................................................................................ Q G I H H L F L N L R M T E S G G N Y V S K G A V K A F P L N L P R L P S F M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................... acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaacctttgcaaaggggagattcaatggacggagaggggaaaaaatgtannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggcggcaagttcaaaaagcgataatcgcattggttaatcaggcttcctcaaataaaagggaagtctgatttttacatccatgcaaggagcaatatgatataatatgt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaacctttgcaaaggggagattcaatggacggagaggggaaaaaatgtannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggcggcaagttcaaaaagcgataatcgcattggttaatcaggcttcctcaaataaaagggaagtctgatttttacatccatgcaaggagcaatatgatataatatgt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 56 HP_score: -3.4 Tail_Score: -3.92373 Start: 142 End: 154 Full_Region: agggtccgaaacaat gaga ctaaa tctc tttctgcatcacata .............................................................................................................................................gagactaaatctc............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 79 HP_score: -6.8 Tail_Score: -4.66833 Start: 606 End: 616 Full_Region: gcctgattaaccaat gcga tta tcgc tttttgaacttgccg .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gcgattatcgc............................................................................... TransTerm Strand: + Conf: 73 HP_score: -5.5 Tail_Score: -4.67353 Start: 626 End: 656 Full_Region: AATCGCATTGGTTAA TCAGGCTTCCT CAAATAAAA GGGAAGTCTGA TTTTTACATCCATGC .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TCAGGCTTCCTCAAATAAAAGGGAAGTCTGA....................................... Find igs database================================================ Query_seq: PG0827:PG0829|PG0827:PG0829:MATE efflux family protein:hypothetical protein:-><-:885665..886359 695 acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaacctttgcaaaggggagattcaatggacggagaggggaaaaaatgtannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggcggcaagttcaaaaagcgataatcgcattggttaatcaggcttcctcaaataaaagggaagtctgatttttacatccatgcaaggagcaatatgatataatatgt Intra-Species Hit: Count: 2 Min: 1 Max: 695 Len: 695 Subject: NC_002950_PG1444_PG1446|hypothetical protein:MATE efflux family protein|POSITIVE:NEGATIVE|[1529303,1529997]|695 HSP 1 e-value: 1.0E-133 bit: 476.0 Len: 240 Query Start:1 Query End:240 Subject Strand: NEGATIVE Subject Start: 456 Subject End: 695 acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaacctttgcaaaggggagattcaatggacggagaggggaaaaaatgta....................................................................................................................................................................................................................................................................................................................................................................................................................................................................... acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaacctttgcaaaggggagattcaatggacggagaggggaaaaaatgta....................................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-52 bit: 208.0 Len: 105 Query Start:591 Query End:695 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 105 ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cggcaagttcaaaaagcgataatcgcattggttaatcaggcttcctcaaataaaagggaagtctgatttttacatccatgcaaggagcaatatgatataatatgt ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cggcaagttcaaaaagcgataatcgcattggttaatcaggcttcctcaaataaaagggaagtctgatttttacatccatgcaaggagcaatatgatataatatgt Subject: NC_002950_PG0827_PG0829|MATE efflux family protein:hypothetical protein|POSITIVE:NEGATIVE|[885665,886359]|695 HSP 1 e-value: 1.0E-133 bit: 476.0 Len: 240 Query Start:1 Query End:240 Subject Strand: POSITIVE Subject Start: 1 Subject End: 240 acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaacctttgcaaaggggagattcaatggacggagaggggaaaaaatgta....................................................................................................................................................................................................................................................................................................................................................................................................................................................................... acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaacctttgcaaaggggagattcaatggacggagaggggaaaaaatgta....................................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-52 bit: 208.0 Len: 105 Query Start:591 Query End:695 Subject Strand: POSITIVE Subject Start: 591 Subject End: 695 ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cggcaagttcaaaaagcgataatcgcattggttaatcaggcttcctcaaataaaagggaagtctgatttttacatccatgcaaggagcaatatgatataatatgt ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cggcaagttcaaaaagcgataatcgcattggttaatcaggcttcctcaaataaaagggaagtctgatttttacatccatgcaaggagcaatatgatataatatgt Inter-species Hit: Count: 2 Min: 1 Max: 240 Len: 240 Subject: pint_PI0395_PI0396|Na+-driven multidrug efflux pump:possible tetracycline resistance element mobilization regulatory protein, C-terminal fragment|POSITIVE:POSITIVE|[361845,362132]|288 HSP 1 e-value: 1.0E-130 bit: 468.0 Len: 240 Query Start:1 Query End:240 Subject Strand: POSITIVE Subject Start: 4 Subject End: 243 acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaacctttgcaaaggggagattcaatggacggagaggggaaaaaatgta....................................................................................................................................................................................................................................................................................................................................................................................................................................................................... acataaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatacacgcttttgcctgcaaccgttgcaaaggggagattcaatggacggagaggggaaaaaatgta....................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF0922_TF0923|MATE efflux family protein:tetracycline resistance element mobilization regulatory protein|POSITIVE:POSITIVE|[961147,961395]|249 HSP 1 e-value: 6.0E-37 bit: 157.0 Len: 175 Query Start:4 Query End:178 Subject Strand: POSITIVE Subject Start: 13 Subject End: 187 ...taaaagatatgattatgctaagacttactcaaactgaagactttaagacaataatctctttatgctctacaaaaggacagatacaaaaagcgtcagacaactttattctgaagattattgccctatgtgatgcagaaagagatttagtctcattgtttcggaccctccgttatac..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ...taaaagatatgattatgctaagacttactcaaacagaaaactttaagataatgctctctttttgttctacaaaagaacagatacaaaaggcgtcagacaactttatattgaaggttgttgctctatgtgatacagaagaagatactgtctcgttgtttcgtatcctccgctatac..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACAUAAAAGAUAUGAUUAUGCUAAGACUUACUCAAACUGAAGACUUUAAGACAAUAAUCUCUUUAUGCUCUACAAAAGGACAGAUACAAAAAGCGUCAGACAACUUUAUUCUGAAGAUUAUUGCCCUAUGUGAUGCAGAAAGAGAUUUAGUCUCAUUGUUUCGGACCCUCCGUUAUACACGCUUUUGCCUGCAACCUUUGCAAAGGGGAGAUUCAAUGGACGGAGAGGGGAAAAAAUGUA ((((................................((((.(((..(.((((...(((((((((.(((..((((..(((...((((.........(((((.........)))))....))))..))).))))..))).)))))))))..)))).)..))))))).(((((((((........((((.((((((.....)))..))).)))).......))))))).)).......)))). (-53.88) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACAUUUUUUCCCCUCUCCGUCCAUUGAAUCUCCCCUUUGCAAAGGUUGCAGGCAAAAGCGUGUAUAACGGAGGGUCCGAAACAAUGAGACUAAAUCUCUUUCUGCAUCACAUAGGGCAAUAAUCUUCAGAAUAAAGUUGUCUGACGCUUUUUGUAUCUGUCCUUUUGUAGAGCAUAAAGAGAUUAUUGUCUUAAAGUCUUCAGUUUGAGUAAGUCUUAGCAUAAUCAUAUCUUUUAUGU ..((((.((((..((((((((.............((((....)))).((((.((....)).))))..))))))))...)))).)))).((((.(((((((((.(((.(((((.((((((.......(((((.........))))).((.....))...))))))..))).))))).))))))))).....((((((.(....).))))))..))))...((((((.........)))))) (-60.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 394 230 181 120 62 NC_002950:PG0145|5end_hypothetical_protein_167831..168061_POSITIVE 368 230 181 131 78 NC_002950:PG1176|5end_ABC_transporter,_ATP-binding_protein,_putative_1257644..1257874_NEGATIVE 363 230 182 204 148 NC_002950:PG0199|5end_TatD_family_protein_238137..238367_NEGATIVE 350 230 181 63 7 NC_002950:PG0171|5end_5'-nucleotidase_family_protein_191724..191954_POSITIVE 349 228 184 231 197 NC_002950:PG0221|5end_hypothetical_protein_255834..256064_NEGATIVE 330 229 187 132 81 NC_002950:PG0031|5end_hypothetical_protein_37579..37809_NEGATIVE 329 210 162 191 149 NC_002950:PG0400|5end_hypothetical_protein_437528..437758_NEGATIVE 328 210 161 162 102 NC_002950:PG0026|5end_hypothetical_protein_29234..29464_POSITIVE 325 230 181 62 10 NC_002950:PG0285|5end_hypothetical_protein_318459..318689_POSITIVE 324 225 181 65 24 NC_002950:PG1861|5end_hypothetical_protein_1960057..1960287_NEGATIVE 320 230 181 224 162 NC_002950:PG1641|5end_phosphotyrosine_protein_phosphatase_1721763..1721993_NEGATIVE 318 208 161 57 13 NC_002950:PG1813|5end_ferredoxin,_4Fe-4S_1908374..1908604_NEGATIVE 314 187 142 55 9 NC_002950:PG1840|5end_hypothetical_protein_1937102..1937332_POSITIVE 314 210 167 146 95 NC_002950:PG1521|5end_O-succinylbenzoic_acid--CoA_ligase_1599103..1599333_NEGATIVE 310 239 191 169 108 NC_002950:PG1654|5end_D-alanyl-D-alanine_dipeptidase_1736458..1736688_POSITIVE 310 238 191 217 159 NC_002950:PG0401|5end_hypothetical_protein_439306..439536_NEGATIVE 310 230 181 57 4 NC_002950:PG0027|5end_hypothetical_protein_32681..32911_POSITIVE 309 225 181 145 110 NC_002950:PG0984|5end_hypothetical_protein_1046919..1047149_NEGATIVE 307 228 187 131 88 NC_002950:PG1982|5end_CRISPR-associated_protein_Cas1_2072620..2072850_NEGATIVE 307 224 181 138 79 NC_002950:PG0428|5end_hypothetical_protein_465977..466207_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 403 229 181 147 100 NC_002950:PG1497|3end_DNA-binding_protein,_histone-like_family_1574635..1574785_POSITIVE 335 209 161 106 63 NC_002950:PG1619|3end_biotin_synthesis_protein_BioC,_putative_1699415..1699565_POSITIVE 332 210 162 75 20 NC_002950:PG1392|3end_rod_shape-determining_protein_RodA,_putative_1472959..1473109_NEGATIVE 330 229 187 84 33 NC_002950:PG0032|3end_beta-mannosidase,_putative_37627..37777_NEGATIVE 324 225 181 52 11 NC_002950:PG1862|3end_hypothetical_protein_1960070..1960220_NEGATIVE 319 225 181 56 5 NC_002950:PG1896|3end_S-adenosylmethionine_synthetase_1995955..1996105_POSITIVE 319 176 131 94 41 NC_002950:PG0199|3end_TatD_family_protein_237424..237574_NEGATIVE 318 225 181 151 103 NC_002950:PG2040|3end_DNA-binding_protein,_histone-like_family_2138398..2138548_NEGATIVE 316 227 184 151 118 NC_002950:PG0222|3end_DNA-binding_protein,_histone-like_family_255913..256063_NEGATIVE 315 170 123 146 104 NC_002950:PG1840|3end_hypothetical_protein_1937295..1937445_POSITIVE 315 210 161 129 77 NC_002950:PG1024|3end_hypothetical_protein_1086876..1087026_POSITIVE 314 210 167 149 98 NC_002950:PG1522|3end_mandelate_racemase/muconate_lactonizing_enzyme_family_protein_1599100..1599250_NEGATIVE 314 229 181 143 103 NC_002950:PG0934|3end_hypothetical_protein_992414..992564_NEGATIVE 310 239 191 89 28 NC_002950:PG1653|3end_hypothetical_protein_1736458..1736608_POSITIVE 310 238 191 70 12 NC_002950:PG0403|3end_hypothetical_protein_439453..439603_NEGATIVE 307 228 187 70 27 NC_002950:PG1983|3end_CRISPR-associated_TM1791_family_protein_2072681..2072831_NEGATIVE 307 210 163 128 80 NC_002950:PG1684|3end_hypothetical_protein_1771483..1771633_POSITIVE 307 209 164 59 13 NC_002950:PG0915|3end_hypothetical_protein_977057..977207_POSITIVE 305 171 134 73 34 NC_002950:PG0651|3end_HDIG_domain_protein_703071..703221_NEGATIVE 302 228 181 45 11 NC_002950:PG1879|3end_hypothetical_protein_1975550..1975700_POSITIVE