Origin IGS: taaatggcgcgcttcttcatctatctggcatacaacggcactcgctacagcggctggcaaacgcagcctaatgcccccggtgtgcagcagaccgtagaggaagccatatctaccatcgtgcgccaacctgtcggggtcgtcggagcaggacgaaccgatgcaggagttcatgcccacgagatggtagcacatgccgatcttccctgcgatacgccggcggaagctgccaccctgacagaaaggctcaataaactcctcccacgcgacatcgtcatataccgaatggctcctgtcaaagccgatgcccacgcgcgcttcgatgccatctcccgaacttaccactactatctgaccgagcagaaagatccttttatggaaggcttgatgctgaagacctatcgtaagctcgacttcggacgcatgaatgaagcggcggctcttcttccgcgatatatcgacttcacgagcttcagcaaactgcataccgacgtcaaaacgaacaactgccgtataacggaagcccgatggatgccacttgcacagaccggtcaatgggtcttttccatcacggcggacagattcctgcgcaatatggtacgtgccatagtcggcacgctcttccatgtggggacgggcaagctatccatcacagactttcgggacatcatcgaatcgcaggatcgcagccgtgccggctcttcggctccggctcatgccctctatctggagagggtagtttacccgtcggatctgtttctctgacagcaccgccgtatcttcgatacgaaggcagctttccatccggcattacttttttctctgtcgcttcgccggcatccgtttcgcttctgtcaatatctattctttgactgacattttattagtacacctcagatggctttgcgcaaaattgttataatacacatggagatgtacggttggcattatatcccggagtgcg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cgcactccgggatataatgccaaccgtacatctccatgtgtattataacaattttgcgcaaagccatctgaggtgtactaataaaatgtcagtcaaagaatagatattgacagaagcgaaacggatgccggcgaagcgacagagaaaaaagtaatgccggatggaaagctgccttcgtatcgaagatacggcggtgctgtcagagaaacagatccgacgggtaaactaccctctccagatagagggcatgagccggagccgaagagccggcacggctgcgatcctgcgattcgatgatgtcccgaaagtctgtgatggatagcttgcccgtccccacatggaagagcgtgccgactatggcacgtaccatattgcgcaggaatctgtccgccgtgatggaaaagacccattgaccggtctgtgcaagtggcatccatcgggcttccgttatacggcagttgttcgttttgacgtcggtatgcagtttgctgaagctcgtgaagtcgatatatcgcggaagaagagccgccgcttcattcatgcgtccgaagtcgagcttacgataggtcttcagcatcaagccttccataaaaggatctttctgctcggtcagatagtagtggtaagttcgggagatggcatcgaagcgcgcgtgggcatcggctttgacaggagccattcggtatatgacgatgtcgcgtgggaggagtttattgagcctttctgtcagggtggcagcttccgccggcgtatcgcagggaagatcggcatgtgctaccatctcgtgggcatgaactcctgcatcggttcgtcctgctccgacgaccccgacaggttggcgcacgatggtagatatggcttcctctacggtctgctgcacaccgggggcattaggctgcgtttgccagccgctgtagcgagtgccgttgtatgccagatagatgaagaagcgcgccattta Mask Tandem Repeat Region ================================================ taaatggcgcgcttcttcatctatctggcatacaacggcactcgctacagcggctggcaaacgcagcctaatgcccccggtgtgcagcagaccgtagaggaagccatatctaccatcgtgcgccaacctgtcggggtcgtcggagcaggacgaaccgatgcaggagttcatgcccacgagatggtagcacatgccgatcttccctgcgatacgccggcggaagctgccaccctgacagaaaggctcaataaactcctcccacgcgacatcgtcatataccgaatggctcctgtcaaagccgatgcccacgcgcgcttcgatgccatctcccgaacttaccactactatctgaccgagcagaaagatccttttatggaaggcttgatgctgaagacctatcgtaagctcgacttcggacgcatgaatgaagcggcggctcttcttccgcgatatatcgacttcacgagcttcagcaaactgcataccgacgtcaaaacgaacaactgccgtataacggaagcccgatggatgccacttgcacagaccggtcaatgggtcttttccatcacggcggacagattcctgcgcaatatggtacgtgccatagtcggcacgctcttccatgtggggacgggcaagctatccatcacagactttcgggacatcatcgaatcgcaggatcgcagccgtgccggctcttcggctccggctcatgccctctatctggagagggtagtttacccgtcggatctgtttctctgacagcaccgccgtatcttcgatacgaaggcagctttccatccggcattacttttttctctgtcgcttcgccggcatccgtttcgcttctgtcaatatctattctttgactgacattttattagtacacctcagatggctttgcgcaaaattgttataatacacatggagatgtacggttggcattatatcccggagtgcg Find is-nt database================================================ Query_seq: PG0754:PG0756|PG0754:PG0756:DNA topoisomerase I:hypothetical protein:-><-:803450..804410 961 taaatggcgcgcttcttcatctatctggcatacaacggcactcgctacagcggctggcaaacgcagcctaatgcccccggtgtgcagcagaccgtagaggaagccatatctaccatcgtgcgccaacctgtcggggtcgtcggagcaggacgaaccgatgcaggagttcatgcccacgagatggtagcacatgccgatcttccctgcgatacgccggcggaagctgccaccctgacagaaaggctcaataaactcctcccacgcgacatcgtcatataccgaatggctcctgtcaaagccgatgcccacgcgcgcttcgatgccatctcccgaacttaccactactatctgaccgagcagaaagatccttttatggaaggcttgatgctgaagacctatcgtaagctcgacttcggacgcatgaatgaagcggcggctcttcttccgcgatatatcgacttcacgagcttcagcaaactgcataccgacgtcaaaacgaacaactgccgtataacggaagcccgatggatgccacttgcacagaccggtcaatgggtcttttccatcacggcggacagattcctgcgcaatatggtacgtgccatagtcggcacgctcttccatgtggggacgggcaagctatccatcacagactttcgggacatcatcgaatcgcaggatcgcagccgtgccggctcttcggctccggctcatgccctctatctggagagggtagtttacccgtcggatctgtttctctgacagcaccgccgtatcttcgatacgaaggcagctttccatccggcattacttttttctctgtcgcttcgccggcatccgtttcgcttctgtcaatatctattctttgactgacattttattagtacacctcagatggctttgcgcaaaattgttataatacacatggagatgtacggttggcattatatcccggagtgcg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0754:PG0756|PG0754:PG0756:DNA topoisomerase I:hypothetical protein:-><-:803450..804410 961 taaatggcgcgcttcttcatctatctggcatacaacggcactcgctacagcggctggcaaacgcagcctaatgcccccggtgtgcagcagaccgtagaggaagccatatctaccatcgtgcgccaacctgtcggggtcgtcggagcaggacgaaccgatgcaggagttcatgcccacgagatggtagcacatgccgatcttccctgcgatacgccggcggaagctgccaccctgacagaaaggctcaataaactcctcccacgcgacatcgtcatataccgaatggctcctgtcaaagccgatgcccacgcgcgcttcgatgccatctcccgaacttaccactactatctgaccgagcagaaagatccttttatggaaggcttgatgctgaagacctatcgtaagctcgacttcggacgcatgaatgaagcggcggctcttcttccgcgatatatcgacttcacgagcttcagcaaactgcataccgacgtcaaaacgaacaactgccgtataacggaagcccgatggatgccacttgcacagaccggtcaatgggtcttttccatcacggcggacagattcctgcgcaatatggtacgtgccatagtcggcacgctcttccatgtggggacgggcaagctatccatcacagactttcgggacatcatcgaatcgcaggatcgcagccgtgccggctcttcggctccggctcatgccctctatctggagagggtagtttacccgtcggatctgtttctctgacagcaccgccgtatcttcgatacgaaggcagctttccatccggcattacttttttctctgtcgcttcgccggcatccgtttcgcttctgtcaatatctattctttgactgacattttattagtacacctcagatggctttgcgcaaaattgttataatacacatggagatgtacggttggcattatatcccggagtgcg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 25 Max: 744 Len: 720 Subject: gi|89109138|ref|AP_002918.1| pseudouridylate synthase I [Escherichia coli W3110] HSP 1 e-value: 1.0E-28 bit: 123.0 Len: 720 Query Start:25 Query End:744 Subject Strand: null Subject Start: 16 Subject End: 253 ........................ L A Y N G T R Y S G W Q T Q P N A P G V Q Q T V E E A I S T I V R Q P V G V V G A G R T D A G V H A H E M V A H A D L P C D T P A E A A T L T E R L N K L L P R D I V I Y R M A P V K A D A H A R F D A I S R T Y H Y - - Y L T E Q K D P F M E G L M L K T Y R K L D F G R M N E A A A L L P R Y I D F T S F S K L H T D V K T - - - N N C R I T E A R W M P L A Q T G Q W V F S I T A D R F L R N M V R A I V G T L F H V G T G K L S I T D F R D I I E S Q D R S R A G S S A P A H A L Y L E R V V Y P ......................................................................................................................................................................................................................... ........................ I E Y D G S K Y Y G W Q R Q N E V R S V Q E K L E K A L S Q V A N E P I T V F C A G R T D A G V H G T G Q V V H F E T T A L R K D A A W T L - - G V N A N L P G D I A V R W V K T V P D D F H A R F S A T A R R Y R Y I I Y N H R L R P A V L S K G V T H F Y E P L D A E R M H R A A Q C L L G E N D F T S F R A V Q C Q S R T P W R N V M H I N V T R H G P Y V - - - - - V V D I K A N A F V H H M V R N I V G S L M E V G A H N Q P E S W I A E L L A A K D R T L A A A T A K A E G L Y L V A V D Y P ......................................................................................................................................................................................................................... Find nr database================================================ Query_seq: PG0754:PG0756|PG0754:PG0756:DNA topoisomerase I:hypothetical protein:-><-:803450..804410 961 taaatggcgcgcttcttcatctatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcggatctgtttctctgacagcaccgccgtatcttcgatacgaaggcagctttccatccggcattacttttttctctgtcgcttcgccggcatccgtttcgcttctgtcaatatctattctttgactgacattttattagtacacctcagatggctttgcgcaaaattgttataatacacatggagatgtacggttggcattatatcccggagtgcg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaatggcgcgcttcttcatctatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcggatctgtttctctgacagcaccgccgtatcttcgatacgaaggcagctttccatccggcattacttttttctctgtcgcttcgccggcatccgtttcgcttctgtcaatatctattctttgactgacattttattagtacacctcagatggctttgcgcaaaattgttataatacacatggagatgtacggttggcattatatcccggagtgcg Predict ORF larger than 30AA ================================================ Protein_Len: 47 Strand: + Start: 108 End: 248 ........................................................................................................... M Y H R A P T C R G R R S R T N R C R S S C P R D G S T C R S S L R Y A G G S C H P D R K A Q ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 34 Strand: + Start: 248 End: 349 ....................................................................................................................................................................................................................................................... M N S S H A T S S Y T E W L L S K P M P T R A S M P S P E L T T T I .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 42 Strand: + Start: 276 End: 401 ................................................................................................................................................................................................................................................................................... M P N G S C Q S R C P R A L R C H L P N L P L L S D R A E R S F Y G R L D A E D L S ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 37 Strand: + Start: 623 End: 733 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M W G R A S Y P S Q T F G T S S N R R I A A V P A L R L R L M P S I W R G .................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 4 End: 759 ... M A R F F I Y L A Y N G T R Y S G W Q T Q P N A P G V Q Q T V E E A I S T I V R Q P V G V V G A G R T D A G V H A H E M .......................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 450 End: 866 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M Y R L H E L Q Q T A Y R R Q N E Q L P Y N G S P M D A T C T D R S M G L F H H G G Q I P A Q Y G T C H S R H A L P C G ............................................................................................... Protein_Len: 32 Strand: + Start: 775 End: 870 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M F D T K A A F H P A L L F S L S L R R H P F R F C Q Y L F F D ........................................................................................... Protein_Len: 56 Strand: + Start: 749 End: 916 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M C F S D S T A V S S I R R Q L S I R H Y F F L C R F A G I R F A S V N I Y S L T D I L L V H L R W L C A K L L ............................................. Protein_Len: 60 Strand: - Start: 668 End: 862 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... C G H R S K P E P E H G R D P S P L K G T P D T E R V A G G Y R R Y S P L K G D P M V K K E T A E G A D T E S R D I D M ................................................................................................... Protein_Len: 53 Strand: - Start: 643 End: 801 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. G D C V K P V D D F R L I A A T G A R R S R S M G E I Q L P Y N V R R I Q K E S L V A T D E I R L C S E M ................................................................................................................................................................ Protein_Len: 45 Strand: - Start: 552 End: 686 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... H T K E M V A S L N R R L I T R A M T P V S K W T P V P L S D M V S K R S M M S D C S R M ................................................................................................................................................................................................................................................................................... Protein_Len: 44 Strand: - Start: 399 End: 530 .............................................................................................................................................................................................................................................................................................................................................................................................................. R L S S K P R M F S A A A R R G R Y I S K V L K L L S C V S T L V F L Q R I V S A R H M ............................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 52 Strand: - Start: 171 End: 326 .......................................................................................................................................................................... A W S I T A C A S R G Q S V G A S A A V R V S L S L L S R G R S M T M Y R I A G T L A S A W A R K S A M ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 42 Strand: - Start: 113 End: 238 ................................................................................................................ W R A G V Q R P R R L L V F R H L L E H G R S P L V H R D E R R Y A P P L Q W G S M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 1 End: 624 R S I D V E R A E A F Q M G V D F R V V A T Y R F G S P H W K C L G T L P D K G D R R V S E Q A I H Y T G Y D A R E E M ................................................................................................................................................................................................................................................................................................................................................. taaatggcgcgcttcttcatctatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcggatctgtttctctgacagcaccgccgtatcttcgatacgaaggcagctttccatccggcattacttttttctctgtcgcttcgccggcatccgtttcgcttctgtcaatatctattctttgactgacattttattagtacacctcagatggctttgcgcaaaattgttataatacacatggagatgtacggttggcattatatcccggagtgcg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaatggcgcgcttcttcatctatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcggatctgtttctctgacagcaccgccgtatcttcgatacgaaggcagctttccatccggcattacttttttctctgtcgcttcgccggcatccgtttcgcttctgtcaatatctattctttgactgacattttattagtacacctcagatggctttgcgcaaaattgttataatacacatggagatgtacggttggcattatatcccggagtgcg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0754:PG0756|PG0754:PG0756:DNA topoisomerase I:hypothetical protein:-><-:803450..804410 961 taaatggcgcgcttcttcatctatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcggatctgtttctctgacagcaccgccgtatcttcgatacgaaggcagctttccatccggcattacttttttctctgtcgcttcgccggcatccgtttcgcttctgtcaatatctattctttgactgacattttattagtacacctcagatggctttgcgcaaaattgttataatacacatggagatgtacggttggcattatatcccggagtgcg Intra-Species Hit: Count: 1 Min: 745 Max: 961 Len: 217 Subject: NC_002950_PG0754_PG0756|DNA topoisomerase I:hypothetical protein|POSITIVE:NEGATIVE|[803450,804410]|961 HSP 1 e-value: 1.0E-119 bit: 430.0 Len: 217 Query Start:745 Query End:961 Subject Strand: POSITIVE Subject Start: 745 Subject End: 961 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tcggatctgtttctctgacagcaccgccgtatcttcgatacgaaggcagctttccatccggcattacttttttctctgtcgcttcgccggcatccgtttcgcttctgtcaatatctattctttgactgacattttattagtacacctcagatggctttgcgcaaaattgttataatacacatggagatgtacggttggcattatatcccggagtgcg ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tcggatctgtttctctgacagcaccgccgtatcttcgatacgaaggcagctttccatccggcattacttttttctctgtcgcttcgccggcatccgtttcgcttctgtcaatatctattctttgactgacattttattagtacacctcagatggctttgcgcaaaattgttataatacacatggagatgtacggttggcattatatcccggagtgcg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCGGAUCUGUUUCUCUGACAGCACCGCCGUAUCUUCGAUACGAAGGCAGCUUUCCAUCCGGCAUUACUUUUUUCUCUGUCGCUUCGCCGGCAUCCGUUUCGCUUCUGUCAAUAUCUAUUCUUUGACUGACAUUUUAUUAGUACACCUCAGAUGGCUUUGCGCAAAAUUGUUAUAAUACACAUGGAGAUGUACGGUUGGCAUUAUAUCCCGGAGUGCG .(((..(((((.....)))))..))).((((((...))))))......((.((((...(((((.............)))))....((((((............((.(((((..........))))).))..........(((((.(((.....((.....)).....(((......)))....))).))))).))))))..........)))).)). (-48.22) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGCACUCCGGGAUAUAAUGCCAACCGUACAUCUCCAUGUGUAUUAUAACAAUUUUGCGCAAAGCCAUCUGAGGUGUACUAAUAAAAUGUCAGUCAAAGAAUAGAUAUUGACAGAAGCGAAACGGAUGCCGGCGAAGCGACAGAGAAAAAAGUAAUGCCGGAUGGAAAGCUGCCUUCGUAUCGAAGAUACGGCGGUGCUGUCAGAGAAACAGAUCCGA (((.....((.((...)).))....(((((((((..((((((..((....))..)))))).........)))))))))........((((((((........)))..)))))...)))...(((((.((((((..((.............))..))))))...((.(((.(((.(((((((...))))))).)))))).))..........))))). (-57.62) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 440 89 43 224 162 NC_002950:PG0987|5end_hypothetical_protein_1048002..1048232_POSITIVE 430 130 81 105 52 NC_002950:PG2114|5end_hypothetical_protein_2224158..2224388_NEGATIVE 413 90 41 106 53 NC_002950:PG0897|5end_alpha-amylase_family_protein_962472..962702_NEGATIVE 396 50 4 64 11 NC_002950:PG0481|5end_2-amino-3-ketobutyrate_coenzyme_A_ligase_522622..522852_NEGATIVE 388 98 51 174 112 NC_002950:PG1301|5end_hypothetical_protein_1379921..1380151_NEGATIVE 383 49 3 203 151 NC_002950:PG1285|5end_glucosamine-6-phosphate_deaminase_1362894..1363124_POSITIVE 377 59 11 56 2 NC_002950:PG1879|5end_hypothetical_protein_1974451..1974681_POSITIVE 375 62 21 62 27 NC_002950:PG0553|5end_extracellular_protease,_putative_611189..611419_NEGATIVE 369 50 2 197 145 NC_002950:PG0744|5end_RNA_methyltransferase,_TrmH_family_791897..792127_NEGATIVE 368 95 56 194 160 NC_002950:PG1945|5end_hypothetical_protein_2031697..2031927_POSITIVE 368 60 12 69 4 NC_002950:PG0738|5end_cytidine/deoxycytidylate_deaminase_family_protein_786463..786693_POSITIVE 364 90 41 205 169 NC_002950:PG1719|5end_ABC_transporter,_ATP-binding_protein,_MsbA_family_1806749..1806979_POSITIVE 359 108 69 169 122 NC_002950:PG2029|5end_hypothetical_protein_2126543..2126773_POSITIVE 358 90 44 217 174 NC_002950:PG0221|5end_hypothetical_protein_255834..256064_NEGATIVE 354 55 14 214 167 NC_002950:PG1355|5end_acyltransferase,_putative_1435292..1435522_NEGATIVE 354 108 61 97 57 NC_002950:PG1095|5end_RNA_methyltransferase,_TrmA_family_1160225..1160455_POSITIVE 351 104 77 60 24 NC_002950:PG2040|5end_DNA-binding_protein,_histone-like_family_2138874..2139104_NEGATIVE 351 97 54 222 152 NC_002950:PG0634|5end_ThiJ/PfpI_family_protein_686143..686373_POSITIVE 349 63 25 45 4 NC_002950:PG1481|5end_conjugative_transposon_protein_TraG_1558447..1558677_NEGATIVE 349 109 64 140 88 NC_002950:PG1226|5end_peptidyl-prolyl_cis-trans_isomerase,_cyclophilin-type_1303264..1303494_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 387 90 43 61 1 NC_002950:PG2107|3end_thiamine_biosynthesis_protein_ThiH_2215546..2215696_NEGATIVE 369 50 2 112 60 NC_002950:PG0745|3end_lactoylglutathione_lyase,_putative_791982..792132_NEGATIVE 368 60 12 72 7 NC_002950:PG0737|3end_hypothetical_protein_786546..786696_POSITIVE 368 109 76 132 98 NC_002950:PG0443|3end_hemagglutinin-related_protein_483147..483297_POSITIVE 362 89 45 109 54 NC_002950:PG2126|3end_conserved_hypothetical_protein_TIGR00044_2232438..2232588_NEGATIVE 358 90 44 138 95 NC_002950:PG0222|3end_DNA-binding_protein,_histone-like_family_255913..256063_NEGATIVE 354 55 14 145 98 NC_002950:PG1356|3end_hypothetical_protein_1435361..1435511_NEGATIVE 351 62 21 114 70 NC_002950:PG0799|3end_hypothetical_protein_854599..854749_POSITIVE 349 63 25 48 7 NC_002950:PG1482|3end_conjugative_transposon_protein_TraF_1558444..1558594_NEGATIVE 349 109 64 132 80 NC_002950:PG1225|3end_ABC_transporter,_ATP-binding_protein_1303336..1303486_POSITIVE 343 63 23 68 29 NC_002950:PG1112|3end_hypothetical_protein_1185571..1185721_POSITIVE 342 50 2 73 6 NC_002950:PG1854|3end_5-formyltetrahydrofolate_cyclo-ligase_family_protein_1953325..1953475_NEGATIVE 342 88 45 125 69 NC_002950:PG1162|3end_ATP:cob(I)alamin_adenosyltransferase,_putative_1241473..1241623_NEGATIVE 341 117 76 83 26 NC_002950:PG0224|3end_hypothetical_protein_257614..257764_NEGATIVE 340 118 73 38 2 NC_002950:PG0366|3end_hypothetical_protein_400255..400405_NEGATIVE 339 50 2 151 104 NC_002950:PG1687|3end_HIT_family_protein_1773812..1773962_NEGATIVE 339 63 25 151 119 NC_002950:PG1353|3end_orotate_phosphoribosyltransferase_1433201..1433351_NEGATIVE 338 110 66 101 30 NC_002950:PG2140|3end_ribosomal_protein_L32_2246399..2246549_POSITIVE 338 110 65 136 65 NC_002950:PG1675|3end_hypothetical_protein_1760360..1760510_POSITIVE 337 93 51 149 94 NC_002950:PG1302|3end_hypothetical_protein_1379939..1380089_NEGATIVE