Origin IGS: ctgagagaataatcttcaattctttatttggtgtgtaagatcaacgccttatgcagcaaagatattgtatttttgcgcgaatcggtatttagaacatctcgttcgtagatttgcataacctacctcacaacaataatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aattattgttgtgaggtaggttatgcaaatctacgaacgagatgttctaaataccgattcgcgcaaaaatacaatatctttgctgcataaggcgttgatcttacacaccaaataaagaattgaagattattctctcag Mask Tandem Repeat Region ================================================ ctgagagaataatcttcaattctttatttggtgtgtaagatcaacgccttatgcagcaaagatattgtatttttgcgcgaatcggtatttagaacatctcgttcgtagatttgcataacctacctcacaacaataatt Find is-nt database================================================ Query_seq: PG0751:PG0752|PG0751:PG0752:porT protein:uracil phosphoribosyltransferase:<-->:797669..797806 138 ctgagagaataatcttcaattctttatttggtgtgtaagatcaacgccttatgcagcaaagatattgtatttttgcgcgaatcggtatttagaacatctcgttcgtagatttgcataacctacctcacaacaataatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0751:PG0752|PG0751:PG0752:porT protein:uracil phosphoribosyltransferase:<-->:797669..797806 138 ctgagagaataatcttcaattctttatttggtgtgtaagatcaacgccttatgcagcaaagatattgtatttttgcgcgaatcggtatttagaacatctcgttcgtagatttgcataacctacctcacaacaataatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0751:PG0752|PG0751:PG0752:porT protein:uracil phosphoribosyltransferase:<-->:797669..797806 138 ctgagagaataatcttcaattctttatttggtgtgtaagatcaacgccttatgcagcaaagatattgtatttttgcgcgaatcggtatttagaacatctcgttcgtagatttgcataacctacctcacaacaataatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctgagagaataatcttcaattctttatttggtgtgtaagatcaacgccttatgcagcaaagatattgtatttttgcgcgaatcggtatttagaacatctcgttcgtagatttgcataacctacctcacaacaataatt Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 26 End: 115 ......................... M W C V R S T P Y A A K I L Y F C A N R Y L E H L V R R F A ....................... Protein_Len: 30 Strand: - Start: 27 End: 116 .......................... K T H L I L A K H L L S I T N K R S D T N L V D R E Y I Q M ...................... ctgagagaataatcttcaattctttatttggtgtgtaagatcaacgccttatgcagcaaagatattgtatttttgcgcgaatcggtatttagaacatctcgttcgtagatttgcataacctacctcacaacaataatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctgagagaataatcttcaattctttatttggtgtgtaagatcaacgccttatgcagcaaagatattgtatttttgcgcgaatcggtatttagaacatctcgttcgtagatttgcataacctacctcacaacaataatt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0751:PG0752|PG0751:PG0752:porT protein:uracil phosphoribosyltransferase:<-->:797669..797806 138 ctgagagaataatcttcaattctttatttggtgtgtaagatcaacgccttatgcagcaaagatattgtatttttgcgcgaatcggtatttagaacatctcgttcgtagatttgcataacctacctcacaacaataatt Intra-Species Hit: Count: 1 Min: 1 Max: 138 Len: 138 Subject: NC_002950_PG0751_PG0752|porT protein:uracil phosphoribosyltransferase|NEGATIVE:POSITIVE|[797669, 797806]|138 HSP 1 e-value: 7.0E-73 bit: 274.0 Len: 138 Query Start:1 Query End:138 Subject Strand: POSITIVE Subject Start: 1 Subject End: 138 ctgagagaataatcttcaattctttatttggtgtgtaagatcaacgccttatgcagcaaagatattgtatttttgcgcgaatcggtatttagaacatctcgttcgtagatttgcataacctacctcacaacaataatt ctgagagaataatcttcaattctttatttggtgtgtaagatcaacgccttatgcagcaaagatattgtatttttgcgcgaatcggtatttagaacatctcgttcgtagatttgcataacctacctcacaacaataatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUGAGAGAAUAAUCUUCAAUUCUUUAUUUGGUGUGUAAGAUCAACGCCUUAUGCAGCAAAGAUAUUGUAUUUUUGCGCGAAUCGGUAUUUAGAACAUCUCGUUCGUAGAUUUGCAUAACCUACCUCACAACAAUAAUU .((((.((((........)))).......((((((((.((((...(((...(((.(((((((((...))))))))))))....))).....((((.....))))...))))))))).)))...))))........... (-28.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUAUUGUUGUGAGGUAGGUUAUGCAAAUCUACGAACGAGAUGUUCUAAAUACCGAUUCGCGCAAAAAUACAAUAUCUUUGCUGCAUAAGGCGUUGAUCUUACACACCAAAUAAAGAAUUGAAGAUUAUUCUCUCAG ..(((((...(((..(((((..((((........((((.....)))).............(((((((.(((...))).))))).)).....))))....))))).)))..))))).....((((((....))).))). (-23.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 878 80 31 171 122 NC_002950:PG0751|5end_porT_protein_797578..797808_NEGATIVE 318 88 45 210 171 NC_002950:PG0893|5end_hydroxylamine_reductase_955853..956083_NEGATIVE 301 49 3 204 150 NC_002950:PG1498|5end_hypothetical_protein_1574656..1574886_POSITIVE 297 107 70 168 131 NC_002950:PG2136|5end_hypothetical_protein_2243360..2243590_POSITIVE 285 79 31 173 125 NC_002950:PG0653|5end_phosphoserine_phosphatase_705454..705684_NEGATIVE 281 120 72 225 170 NC_002950:PG2020|5end_CRISPR-associated_TM1814_family_protein_2112273..2112503_NEGATIVE 279 64 30 184 145 NC_002950:PG1082|5end_phosphotransacetylase_1150683..1150913_NEGATIVE 278 109 61 187 138 NC_002950:PG0356|5end_hypothetical_protein_390611..390841_POSITIVE 275 115 75 187 147 NC_002950:PG0729|5end_D-alanylalanine_synthetase_780818..781048_NEGATIVE 272 117 72 222 172 NC_002950:PG1581|5end_hypothetical_protein_1658368..1658598_POSITIVE 271 117 71 86 42 NC_002950:PG0627|5end_RNA-binding_protein_681435..681665_NEGATIVE 267 98 61 46 16 NC_002950:PG1703|5end_MazG_family_protein_1790753..1790983_NEGATIVE 265 80 31 80 35 NC_002950:PG0297|5end_hypothetical_protein_335518..335748_NEGATIVE 260 86 42 72 22 NC_002950:PG1175|5end_ABC_transporter,_ATP-binding_protein,_putative_1255876..1256106_NEGATIVE 259 130 81 208 152 NC_002950:PG1592|5end_HDIG_domain_protein_1669383..1669613_POSITIVE 258 84 41 44 6 NC_002950:PG1858|5end_flavodoxin_1957035..1957265_NEGATIVE 258 127 81 90 39 NC_002950:PG0132|5end_hypothetical_protein_152879..153109_POSITIVE 256 110 61 80 20 NC_002950:PG0531|5end_NAD(+)_synthetase_581139..581369_NEGATIVE 256 120 74 190 140 NC_002950:PG0002|5end_hexapeptide_transferase_family_protein_1295..1525_POSITIVE 254 76 44 88 60 NC_002950:PG0510|5end_hypothetical_protein_550060..550290_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 301 49 3 103 49 NC_002950:PG1497|3end_DNA-binding_protein,_histone-like_family_1574635..1574785_POSITIVE 297 107 70 141 104 NC_002950:PG2135|3end_lipoprotein,_putative_2243413..2243563_POSITIVE 290 49 1 83 33 NC_002950:PG1112|3end_hypothetical_protein_1185571..1185721_POSITIVE 285 79 31 114 66 NC_002950:PG0654|3end_hypothetical_protein_705513..705663_NEGATIVE 265 105 63 150 109 NC_002950:PG0700|3end_hypothetical_protein_756083..756233_NEGATIVE 261 108 61 64 19 NC_002950:PG0380|3end_excinuclease_ABC_subunit_B_411218..411368_POSITIVE 261 87 43 49 3 NC_002950:PG0202|3end_uroporphyrinogen-III_synthase_HemD,_putative_238767..238917_NEGATIVE 254 76 44 52 24 NC_002950:PG0511|3end_spore_maturation_protein_A/spore_maturation_protein_B_550096..550246_NEGATIVE 253 65 38 76 43 NC_002950:PG0157|3end_regulatory_protein_RecX_180271..180421_POSITIVE 250 49 1 128 80 NC_002950:PG0477|3end_pantoate--beta-alanine_ligase_518752..518902_NEGATIVE 249 80 31 124 73 NC_002950:PG0647|3end_iron_compound_ABC_transporter,_permease_protein_699079..699229_NEGATIVE 248 85 45 38 2 NC_002950:PG1019|3end_lipoprotein,_putative_1080476..1080626_POSITIVE 246 47 4 117 78 NC_002950:PG0958|3end_ribonuclease_BN,_putative_1018135..1018285_NEGATIVE 245 126 83 59 27 NC_002950:PG1910|3end_ribosomal_protein_L17_2010282..2010432_NEGATIVE 243 54 18 116 81 NC_002950:PG1786|3end_hypothetical_protein_1875322..1875472_POSITIVE 242 97 53 126 74 NC_002950:PG1235|3end_epimerase/reductase,_putative_1310300..1310450_NEGATIVE 241 116 74 142 105 NC_002950:PG0358|3end_aspartate_carbamoyltransferase_regulatory_subunit_392902..393052_POSITIVE 240 115 71 67 21 NC_002950:PG2216|3end_hypothetical_protein_2329530..2329680_POSITIVE 240 110 65 52 4 NC_002950:PG0290|3end_hypothetical_protein_325534..325684_POSITIVE 237 86 46 30 2 NC_002950:PG1663|3end_ABC_transporter,_ATP-binding_protein_1745868..1746018_POSITIVE