Origin IGS: aatatccaaaaagcttaagcgtcgataccggcacaaagaggctatggtaacaatcgactgcaaaaatatcctttaccggctaaaactcagataaaatgtgtttttcattgcaaggatatgcgccactgacctgtcgaaaagagcgtgacaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttgtcacgctcttttcgacaggtcagtggcgcatatccttgcaatgaaaaacacattttatctgagttttagccggtaaaggatatttttgcagtcgattgttaccatagcctctttgtgccggtatcgacgcttaagctttttggatatt Mask Tandem Repeat Region ================================================ aatatccaaaaagcttaagcgtcgataccggcacaaagaggctatggtaacaatcgactgcaaaaatatcctttaccggctaaaactcagataaaatgtgtttttcattgcaaggatatgcgccactgacctgtcgaaaagagcgtgacaa Find is-nt database================================================ Query_seq: PG0750:PG0751|PG0750:PG0751:glycosyl transferase, group 2 family protein:porT protein:<-<-:796783..796933 151 aatatccaaaaagcttaagcgtcgataccggcacaaagaggctatggtaacaatcgactgcaaaaatatcctttaccggctaaaactcagataaaatgtgtttttcattgcaaggatatgcgccactgacctgtcgaaaagagcgtgacaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0750:PG0751|PG0750:PG0751:glycosyl transferase, group 2 family protein:porT protein:<-<-:796783..796933 151 aatatccaaaaagcttaagcgtcgataccggcacaaagaggctatggtaacaatcgactgcaaaaatatcctttaccggctaaaactcagataaaatgtgtttttcattgcaaggatatgcgccactgacctgtcgaaaagagcgtgacaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0750:PG0751|PG0750:PG0751:glycosyl transferase, group 2 family protein:porT protein:<-<-:796783..796933 151 aatatccaaaaagcttaagcgtcgataccggcacaaagaggctatggtaacaatcgactgcaaaaatatcctttaccggctaaaactcagataaaatgtgtttttcattgcaaggatatgcgccactgacctgtcgaaaagagcgtgacaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatatccaaaaagcttaagcgtcgataccggcacaaagaggctatggtaacaatcgactgcaaaaatatcctttaccggctaaaactcagataaaatgtgtttttcattgcaaggatatgcgccactgacctgtcgaaaagagcgtgacaa Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 2 End: 91 . M S K K L K R R Y R H K E A M V T I D C K N I L Y R L K L R ............................................................ Protein_Len: 34 Strand: + Start: 25 End: 126 ........................ M P A Q R G Y G N N R L Q K Y P L P A K T Q I K C V F H C K D M R H ......................... aatatccaaaaagcttaagcgtcgataccggcacaaagaggctatggtaacaatcgactgcaaaaatatcctttaccggctaaaactcagataaaatgtgtttttcattgcaaggatatgcgccactgacctgtcgaaaagagcgtgacaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatatccaaaaagcttaagcgtcgataccggcacaaagaggctatggtaacaatcgactgcaaaaatatcctttaccggctaaaactcagataaaatgtgtttttcattgcaaggatatgcgccactgacctgtcgaaaagagcgtgacaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0750:PG0751|PG0750:PG0751:glycosyl transferase, group 2 family protein:porT protein:<-<-:796783..796933 151 aatatccaaaaagcttaagcgtcgataccggcacaaagaggctatggtaacaatcgactgcaaaaatatcctttaccggctaaaactcagataaaatgtgtttttcattgcaaggatatgcgccactgacctgtcgaaaagagcgtgacaa Intra-Species Hit: Count: 1 Min: 1 Max: 151 Len: 151 Subject: NC_002950_PG0750_PG0751|glycosyl transferase, group 2 family protein:porT protein|NEGATIVE:NEGATIVE|[796783,796933]|151 HSP 1 e-value: 1.0E-80 bit: 299.0 Len: 151 Query Start:1 Query End:151 Subject Strand: POSITIVE Subject Start: 1 Subject End: 151 aatatccaaaaagcttaagcgtcgataccggcacaaagaggctatggtaacaatcgactgcaaaaatatcctttaccggctaaaactcagataaaatgtgtttttcattgcaaggatatgcgccactgacctgtcgaaaagagcgtgacaa aatatccaaaaagcttaagcgtcgataccggcacaaagaggctatggtaacaatcgactgcaaaaatatcctttaccggctaaaactcagataaaatgtgtttttcattgcaaggatatgcgccactgacctgtcgaaaagagcgtgacaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAUCCAAAAAGCUUAAGCGUCGAUACCGGCACAAAGAGGCUAUGGUAACAAUCGACUGCAAAAAUAUCCUUUACCGGCUAAAACUCAGAUAAAAUGUGUUUUUCAUUGCAAGGAUAUGCGCCACUGACCUGUCGAAAAGAGCGUGACAA ............((((..((((((((((((((........)))..))))....))))).))....((((((((........(((((.((.......)).))))).......)))))))).....................))))....... (-29.16) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGUCACGCUCUUUUCGACAGGUCAGUGGCGCAUAUCCUUGCAAUGAAAAACACAUUUUAUCUGAGUUUUAGCCGGUAAAGGAUAUUUUUGCAGUCGAUUGUUACCAUAGCCUCUUUGUGCCGGUAUCGACGCUUAAGCUUUUUGGAUAUU ..(((((...(((......)))...)))))..((((((..((.((((((......)))))).(((((.((.(((((((.(((.(((....((((....))))....))).))).....)))))))...)).))))).)).....)))))). (-31.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 972 60 11 151 102 NC_002950:PG0750|5end_glycosyl_transferase,_group_2_family_protein_796692..796922_NEGATIVE 347 57 13 70 20 NC_002950:PG1599|5end_hypothetical_protein_1679087..1679317_POSITIVE 334 62 23 231 191 NC_002950:PG1635|5end_hypothetical_protein_1715861..1716091_NEGATIVE 323 150 101 182 136 NC_002950:PG0007|5end_hypothetical_protein_6913..7143_NEGATIVE 309 60 20 146 113 NC_002950:PG1654|5end_D-alanyl-D-alanine_dipeptidase_1736458..1736688_POSITIVE 308 48 6 79 27 NC_002950:PG0159|5end_endopeptidase_PepO_183164..183394_NEGATIVE 307 100 55 224 176 NC_002950:PG0770|5end_hypothetical_protein_824575..824805_NEGATIVE 304 43 3 50 2 NC_002950:PG1409|5end_hypothetical_protein_1493265..1493495_NEGATIVE 300 59 13 178 127 NC_002950:PG1145|5end_long-chain-fatty-acid--CoA_ligase,_putative_1230305..1230535_NEGATIVE 300 61 23 72 30 NC_002950:PG1143|5end_sugar_dehydrogenase,_UDP-glucose/GDP-mannose_dehydrogenase_family_1227198..1227428_NEGATIVE 299 146 110 67 28 NC_002950:PG0874|5end_mobilizable_transposon,_int_protein_938013..938243_NEGATIVE 298 62 22 225 174 NC_002950:PG1872|5end_urocanate_hydratase_1968911..1969141_NEGATIVE 297 60 11 85 25 NC_002950:PG1576|5end_L-aspartate_oxidase_1653016..1653246_POSITIVE 297 61 21 222 183 NC_002950:PG0952|5end_4-hydroxy-3-methylbut-2-en-1-yl_diphosphate_synthase_1010922..1011152_POSITIVE 295 59 13 206 156 NC_002950:PG1709|5end_ISPg5,_transposase_Orf1_1794914..1795144_POSITIVE 292 60 20 202 158 NC_002950:PG1911|5end_DNA-directed_RNA_polymerase_alpha_subunit_2011762..2011992_NEGATIVE 289 144 101 79 35 NC_002950:PG2145|5end_polysaccharide_deacetylase_2253253..2253483_POSITIVE 287 149 103 141 94 NC_002950:PG1473|5end_conjugative_transposon_protein_TraQ_1550225..1550455_NEGATIVE 287 148 114 170 130 NC_002950:PG1007|5end_transcriptional_regulator,_GntR_family_1066889..1067119_NEGATIVE 285 49 1 69 21 NC_002950:PG1553|5end_CobN/magnesium_chelatase_family_protein_1630858..1631088_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 347 57 13 73 23 NC_002950:PG1598|3end_lipoprotein_signal_peptidase,_putative_1679170..1679320_POSITIVE 336 45 5 94 46 NC_002950:PG0085|3end_alpha-galactosidase_100556..100706_POSITIVE 334 145 110 46 9 NC_002950:PG1274|3end_elongation_factor_P_1352489..1352639_POSITIVE 320 60 13 98 51 NC_002950:PG2140|3end_ribosomal_protein_L32_2246399..2246549_POSITIVE 317 150 103 140 89 NC_002950:PG0572|3end_hypothetical_protein_627434..627584_NEGATIVE 313 148 108 87 46 NC_002950:PG0155|3end_riboflavin_biosynthesis_protein_RibD_177861..178011_NEGATIVE 309 60 20 66 33 NC_002950:PG1653|3end_hypothetical_protein_1736458..1736608_POSITIVE 308 48 6 60 8 NC_002950:PG0160|3end_hypothetical_protein_183183..183333_NEGATIVE 300 58 13 69 12 NC_002950:PG0530|3end_carbamoyl-phosphate_synthase,_large_subunit_579036..579186_POSITIVE 298 59 12 137 88 NC_002950:PG0750|3end_glycosyl_transferase,_group_2_family_protein_795946..796096_NEGATIVE 298 42 2 150 98 NC_002950:PG0308|3end_electron_transport_complex,_RnfABCDGE_type,_A_subunit_343827..343977_POSITIVE 297 61 21 48 9 NC_002950:PG0951|3end_phosphoribosylaminoimidazole_carboxylase,_PurE_protein_1010828..1010978_POSITIVE 288 43 13 34 1 NC_002950:PG0775|3end_acyl-CoA_dehydrogenase_family_protein_827432..827582_NEGATIVE 287 145 110 119 75 NC_002950:PG1761|3end_acetyltransferase,_GNAT_family_1847739..1847889_POSITIVE 287 149 103 128 81 NC_002950:PG1474|3end_conjugative_transposon_protein_TraO_1550238..1550388_NEGATIVE 277 70 22 69 21 NC_002950:PG1250|3end_hypothetical_protein_1327184..1327334_NEGATIVE 277 60 14 146 90 NC_002950:PG1043|3end_ferrous_iron_transport_protein_B_1110675..1110825_NEGATIVE 275 80 31 50 7 NC_002950:PG1776|3end_dnaJ_protein_1866702..1866852_POSITIVE 274 58 12 141 93 NC_002950:PG0744|3end_RNA_methyltransferase,_TrmH_family_791133..791283_NEGATIVE 273 57 15 81 27 NC_002950:PG1850|3end_hypothetical_protein_1949049..1949199_NEGATIVE