Origin IGS: acctaaaaatcacataatgaatacttgtccgaatcgttcctgaaaaaaagaagaaatcaggaaaacggacgtgctaaggtgacaaaaacattccatcctcacaaattgtagccctattggaaagggtaatcagcgaactaaagaggtaaatcgca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgcgatttacctctttagttcgctgattaccctttccaatagggctacaatttgtgaggatggaatgtttttgtcaccttagcacgtccgttttcctgatttcttctttttttcaggaacgattcggacaagtattcattatgtgatttttaggt Mask Tandem Repeat Region ================================================ acctaaaaatcacataatgaatacttgtccgaatcgttcctgaaaaaaagaagaaatcaggaaaacggacgtgctaaggtgacaaaaacattccatcctcacaaattgtagccctattggaaagggtaatcagcgaactaaagaggtaaatcgca Find is-nt database================================================ Query_seq: PG0745:PG0746|PG0745:PG0746:lactoylglutathione lyase, putative:sensor histidine kinase:<-<-:792471..792625 155 acctaaaaatcacataatgaatacttgtccgaatcgttcctgaaaaaaagaagaaatcaggaaaacggacgtgctaaggtgacaaaaacattccatcctcacaaattgtagccctattggaaagggtaatcagcgaactaaagaggtaaatcgca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0745:PG0746|PG0745:PG0746:lactoylglutathione lyase, putative:sensor histidine kinase:<-<-:792471..792625 155 acctaaaaatcacataatgaatacttgtccgaatcgttcctgaaaaaaagaagaaatcaggaaaacggacgtgctaaggtgacaaaaacattccatcctcacaaattgtagccctattggaaagggtaatcagcgaactaaagaggtaaatcgca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0745:PG0746|PG0745:PG0746:lactoylglutathione lyase, putative:sensor histidine kinase:<-<-:792471..792625 155 acctaaaaatcacataatgaatacttgtccgaatcgttcctgaaaaaaagaagaaatcaggaaaacggacgtgctaaggtgacaaaaacattccatcctcacaaattgtagccctattggaaagggtaatcagcgaactaaagaggtaaatcgca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acctaaaaatcacataatgaatacttgtccgaatcgttcctgaaaaaaagaagaaatcaggaaaacggacgtgctaaggtgacaaaaacattccatcctcacaaattgtagccctattggaaagggtaatcagcgaactaaagaggtaaatcgca Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 13 End: 117 ............ M I F V Q G F R E Q F F S S I L F V S T S L H C F C E M R V F Q L G M ...................................... acctaaaaatcacataatgaatacttgtccgaatcgttcctgaaaaaaagaagaaatcaggaaaacggacgtgctaaggtgacaaaaacattccatcctcacaaattgtagccctattggaaagggtaatcagcgaactaaagaggtaaatcgca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acctaaaaatcacataatgaatacttgtccgaatcgttcctgaaaaaaagaagaaatcaggaaaacggacgtgctaaggtgacaaaaacattccatcctcacaaattgtagccctattggaaagggtaatcagcgaactaaagaggtaaatcgca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0745:PG0746|PG0745:PG0746:lactoylglutathione lyase, putative:sensor histidine kinase:<-<-:792471..792625 155 acctaaaaatcacataatgaatacttgtccgaatcgttcctgaaaaaaagaagaaatcaggaaaacggacgtgctaaggtgacaaaaacattccatcctcacaaattgtagccctattggaaagggtaatcagcgaactaaagaggtaaatcgca Intra-Species Hit: Count: 1 Min: 1 Max: 155 Len: 155 Subject: NC_002950_PG0745_PG0746|lactoylglutathione lyase, putative:sensor histidine kinase|NEGATIVE:NEGATIVE|[792471,792625]|155 HSP 1 e-value: 2.0E-70 bit: 266.0 Len: 155 Query Start:1 Query End:155 Subject Strand: POSITIVE Subject Start: 1 Subject End: 155 acctaaaaatcacataatgaatacttgtccgaatcgttcctgnnnnnnngaagaaatcaggaaaacggacgtgctaaggtgacaaaaacattccatcctcacaaattgtagccctattggaaagggtaatcagcgaactaaagaggtaaatcgca acctaaaaatcacataatgaatacttgtccgaatcgttcctgaaaaaaagaagaaatcaggaaaacggacgtgctaaggtgacaaaaacattccatcctcacaaattgtagccctattggaaagggtaatcagcgaactaaagaggtaaatcgca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACCUAAAAAUCACAUAAUGAAUACUUGUCCGAAUCGUUCCUGAAAAAAAGAAGAAAUCAGGAAAACGGACGUGCUAAGGUGACAAAAACAUUCCAUCCUCACAAAUUGUAGCCCUAUUGGAAAGGGUAAUCAGCGAACUAAAGAGGUAAAUCGCA ((((......................(((((.....(((((((.............)))))))..)))))..(((..(....)...........................(((((.......)))))....))).........))))........ (-30.32) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGCGAUUUACCUCUUUAGUUCGCUGAUUACCCUUUCCAAUAGGGCUACAAUUUGUGAGGAUGGAAUGUUUUUGUCACCUUAGCACGUCCGUUUUCCUGAUUUCUUCUUUUUUUCAGGAACGAUUCGGACAAGUAUUCAUUAUGUGAUUUUUAGGU ........((((.........(((((...((((.......)))).........((((.((..........)).)))).)))))..(((((..(((((((.............))))))).....)))))......................)))) (-32.22) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 904 140 91 231 182 NC_002950:PG0745|5end_lactoylglutathione_lyase,_putative_792380..792610_NEGATIVE 372 69 24 64 22 NC_002950:PG0076|5end_N-acetylmuramoyl-L-alanine_amidase,_family_4_91333..91563_NEGATIVE 352 148 111 209 174 NC_002950:PG0224|5end_hypothetical_protein_258252..258482_NEGATIVE 301 108 61 65 24 NC_002950:PG1277|5end_UDP-glucose-6_dehydrogenase,_putative_1353754..1353984_POSITIVE 299 149 105 88 38 NC_002950:PG0684|5end_ABC_transporter,_permease_protein,_putative_737648..737878_POSITIVE 298 100 58 83 47 NC_002950:PG2037|5end_hypothetical_protein_2137812..2138042_NEGATIVE 282 69 24 183 134 NC_002950:PG1676|5end_phosphoenolpyruvate_carboxykinase_1762096..1762326_NEGATIVE 278 120 71 175 121 NC_002950:PG1311|5end_hypothetical_protein_1389709..1389939_POSITIVE 273 147 102 173 127 NC_002950:PG0311|5end_glycosyl_transferase,_group_2_family_protein_346512..346742_NEGATIVE 272 140 92 54 5 NC_002950:PG0583|5end_cell_division_protein_FtsA_640425..640655_POSITIVE 269 95 58 114 68 NC_002950:PG0412|5end_DNA_mismatch_repair_protein_MutL_451440..451670_NEGATIVE 263 145 105 68 26 NC_002950:PG0670|5end_lipoprotein,_putative_720752..720982_POSITIVE 260 140 98 199 147 NC_002950:PG1136|5end_hypothetical_protein_1218327..1218557_NEGATIVE 259 99 57 171 124 NC_002950:PG0334|5end_glycosyl_transferase,_group_2_family_protein_369751..369981_NEGATIVE 254 108 65 166 124 NC_002950:PG1332|5end_NAD(P)_transhydrogenase,_beta_subunit_1412042..1412272_POSITIVE 250 70 26 165 117 NC_002950:PG0011|5end_glycosyl_hydrolase,_family_3_11494..11724_POSITIVE 249 148 103 158 116 NC_002950:PG1592|5end_HDIG_domain_protein_1669383..1669613_POSITIVE 248 137 92 53 3 NC_002950:PG0559|5end_chlorohydrolase_family_protein_617496..617726_NEGATIVE 248 99 58 94 47 NC_002950:PG0035|5end_DNA_polymerase_III,_alpha_subunit_46284..46514_NEGATIVE 246 95 60 42 5 NC_002950:PG0873|5end_mobilizable_transposon,_tnpC_protein_936808..937038_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 342 79 33 84 36 NC_002950:PG1511|3end_hypothetical_protein_1587850..1588000_POSITIVE 299 149 105 61 11 NC_002950:PG0683|3end_ABC_transporter,_permease_protein,_putative_737701..737851_POSITIVE 298 100 58 86 50 NC_002950:PG2038|3end_N-acetylmuramoyl-L-alanine_amidase,_putative_2137809..2137959_NEGATIVE 284 127 88 80 40 NC_002950:PG0769|3end_fibronectin_type_III_domain_protein_822555..822705_NEGATIVE 278 134 94 95 54 NC_002950:PG1321|3end_formate--tetrahydrofolate_ligase_1399656..1399806_NEGATIVE 269 95 58 117 71 NC_002950:PG0413|3end_hypothetical_protein_451437..451587_NEGATIVE 259 99 57 118 71 NC_002950:PG0335|3end_tRNA_delta(2)-isopentenylpyrophosphate_transferase_369804..369954_NEGATIVE 249 120 91 125 100 NC_002950:PG1408|3end_heavy_metal_efflux_pump,_CzcD_family_1492906..1493056_POSITIVE 242 68 23 101 57 NC_002950:PG0165|3end_heat_shock_protein_15_187370..187520_NEGATIVE 238 135 95 137 87 NC_002950:PG2106|3end_hypothetical_protein_2215457..2215607_POSITIVE 237 148 107 67 20 NC_002950:PG0464|3end_adenylosuccinate_synthetase_507603..507753_NEGATIVE 236 135 92 142 100 NC_002950:PG0843|3end_hypothetical_protein_904871..905021_NEGATIVE 232 80 36 74 17 NC_002950:PG2146|3end_hypothetical_protein_2254650..2254800_POSITIVE 232 79 35 139 95 NC_002950:PG2119|3end_oxidoreductase,_Gfo/Idh/MocA_family_2227598..2227748_POSITIVE 232 149 108 77 30 NC_002950:PG1085|3end_hypothetical_protein_1153531..1153681_POSITIVE 232 146 111 43 7 NC_002950:PG0368|3end_DNA_topoisomerase_IV_subunit_B_403259..403409_POSITIVE 231 146 106 147 114 NC_002950:PG1482|3end_conjugative_transposon_protein_TraF_1558444..1558594_NEGATIVE 230 145 105 151 121 NC_002950:PG2040|3end_DNA-binding_protein,_histone-like_family_2138398..2138548_NEGATIVE 229 68 21 101 46 NC_002950:PG2006|3end_hypothetical_protein_2095026..2095176_POSITIVE 225 148 104 142 81 NC_002950:PG1340|3end_L-lactate_permease_1420298..1420448_NEGATIVE