Origin IGS: tctccgccgagttcctcttccacaaaaatccaatttcaccttttgactggcaaaaacacaaagaaacttgttcggtattggtgagctggatttgtgcaatggtctcattatccagagtcagtcgtttgtactttgttctttccatttcaacatcaaagctatgaacgatttttgacagctggcgggctaacccgccagctgtcgttgttataaccatagtgttgcgttagaaaatggaaagtgcagatatggacggcttga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tcaagccgtccatatctgcactttccattttctaacgcaacactatggttataacaacgacagctggcgggttagcccgccagctgtcaaaaatcgttcatagctttgatgttgaaatggaaagaacaaagtacaaacgactgactctggataatgagaccattgcacaaatccagctcaccaataccgaacaagtttctttgtgtttttgccagtcaaaaggtgaaattggatttttgtggaagaggaactcggcggaga Mask Tandem Repeat Region ================================================ tctccgccgagttcctcttccacaaaaatccaatttcaccttttgactggcaaaaacacaaagaaacttgttcggtattggtgagctggatttgtgcaatggtctcattatccagagtcagtcgtttgtactttgttctttccatttcaacatcaaagctatgaacgatttttgacagctggcgggctaacccgccagctgtcgttgttataaccatagtgttgcgttagaaaatggaaagtgcagatatggacggcttga Find is-nt database================================================ Query_seq: PG0740:PG0741|PG0740:PG0741:NLP/P60 family protein:hypothetical protein:-><-:788546..788806 261 tctccgccgagttcctcttccacaaaaatccaatttcaccttttgactggcaaaaacacaaagaaacttgttcggtattggtgagctggatttgtgcaatggtctcattatccagagtcagtcgtttgtactttgttctttccatttcaacatcaaagctatgaacgatttttgacagctggcgggctaacccgccagctgtcgttgttataaccatagtgttgcgttagaaaatggaaagtgcagatatggacggcttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0740:PG0741|PG0740:PG0741:NLP/P60 family protein:hypothetical protein:-><-:788546..788806 261 tctccgccgagttcctcttccacaaaaatccaatttcaccttttgactggcaaaaacacaaagaaacttgttcggtattggtgagctggatttgtgcaatggtctcattatccagagtcagtcgtttgtactttgttctttccatttcaacatcaaagctatgaacgatttttgacagctggcgggctaacccgccagctgtcgttgttataaccatagtgttgcgttagaaaatggaaagtgcagatatggacggcttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0740:PG0741|PG0740:PG0741:NLP/P60 family protein:hypothetical protein:-><-:788546..788806 261 tctccgccgagttcctcttccacaaaaatccaatttcaccttttgactggcaaaaacacaaagaaacttgttcggtattggtgagctggatttgtgcaatggtctcattatccagagtcagtcgtttgtactttgttctttccatttcaacatcaaagctatgaacgatttttgacagctggcgggctaacccgccagctgtcgttgttataaccatagtgttgcgttagaaaatggaaagtgcagatatggacggcttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tctccgccgagttcctcttccacaaaaatccaatttcaccttttgactggcaaaaacacaaagaaacttgttcggtattggtgagctggatttgtgcaatggtctcattatccagagtcagtcgtttgtactttgttctttccatttcaacatcaaagctatgaacgatttttgacagctggcgggctaacccgccagctgtcgttgttataaccatagtgttgcgttagaaaatggaaagtgcagatatggacggcttga Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 33 End: 161 ................................ M S P F D W Q K H K E T C S V L V S W I C A M V S L S R V S R L Y F V L S I S T S K L .................................................................................................... Protein_Len: 42 Strand: + Start: 47 End: 172 .............................................. M A K T Q R N L F G I G E L D L C N G L I I Q S Q S F V L C S F H F N I K A M N D F ......................................................................................... Protein_Len: 39 Strand: + Start: 94 End: 210 ............................................................................................. M Q W S H Y P E S V V C T L F F P F Q H Q S Y E R F L T A G G L T R Q L S L L ................................................... Protein_Len: 60 Strand: - Start: 2 End: 217 . G K S Q C F C L S V Q E T N T L Q I Q A I T E N D L T L R K Y K T R E M E V D F S H V I K S L Q R A L G G A T T T I V M ............................................ Protein_Len: 37 Strand: - Start: 1 End: 111 R R R T G R G C F D L K V K Q S A F V C L F K N P I P S S S K H L P R M M ...................................................................................................................................................... tctccgccgagttcctcttccacaaaaatccaatttcaccttttgactggcaaaaacacaaagaaacttgttcggtattggtgagctggatttgtgcaatggtctcattatccagagtcagtcgtttgtactttgttctttccatttcaacatcaaagctatgaacgatttttgacagctggcgggctaacccgccagctgtcgttgttataaccatagtgttgcgttagaaaatggaaagtgcagatatggacggcttga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tctccgccgagttcctcttccacaaaaatccaatttcaccttttgactggcaaaaacacaaagaaacttgttcggtattggtgagctggatttgtgcaatggtctcattatccagagtcagtcgtttgtactttgttctttccatttcaacatcaaagctatgaacgatttttgacagctggcgggctaacccgccagctgtcgttgttataaccatagtgttgcgttagaaaatggaaagtgcagatatggacggcttga Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -22.4 Tail_Score: -3.79906 Start: 173 End: 204 Full_Region: GCTATGAACGATTTT TGACAGCTGGCGGG CTAA CCCGCCAGCTGTCG TTGTTATAACCATAG ............................................................................................................................................................................TGACAGCTGGCGGGCTAACCCGCCAGCTGTCG......................................................... TransTerm Strand: + Conf: 100 HP_score: -23.7 Tail_Score: -2.87622 Start: 174 End: 203 Full_Region: CTATGAACGATTTTT GACAGCTGGCGGG CTAA CCCGCCAGCTGTC GTTGTTATAACCATA .............................................................................................................................................................................GACAGCTGGCGGGCTAACCCGCCAGCTGTC.......................................................... Find igs database================================================ Query_seq: PG0740:PG0741|PG0740:PG0741:NLP/P60 family protein:hypothetical protein:-><-:788546..788806 261 tctccgccgagttcctcttccacaaaaatccaatttcaccttttgactggcaaaaacacaaagaaacttgttcggtattggtgagctggatttgtgcaatggtctcattatccagagtcagtcgtttgtactttgttctttccatttcaacatcaaagctatgaacgatttttgacagctggcgggctaacccgccagctgtcgttgttataaccatagtgttgcgttagaaaatggaaagtgcagatatggacggcttga Intra-Species Hit: Count: 1 Min: 1 Max: 261 Len: 261 Subject: NC_002950_PG0740_PG0741|NLP/P60 family protein:hypothetical protein|POSITIVE:NEGATIVE|[788546,788806]|261 HSP 1 e-value: 1.0E-146 bit: 517.0 Len: 261 Query Start:1 Query End:261 Subject Strand: POSITIVE Subject Start: 1 Subject End: 261 tctccgccgagttcctcttccacaaaaatccaatttcaccttttgactggcaaaaacacaaagaaacttgttcggtattggtgagctggatttgtgcaatggtctcattatccagagtcagtcgtttgtactttgttctttccatttcaacatcaaagctatgaacgatttttgacagctggcgggctaacccgccagctgtcgttgttataaccatagtgttgcgttagaaaatggaaagtgcagatatggacggcttga tctccgccgagttcctcttccacaaaaatccaatttcaccttttgactggcaaaaacacaaagaaacttgttcggtattggtgagctggatttgtgcaatggtctcattatccagagtcagtcgtttgtactttgttctttccatttcaacatcaaagctatgaacgatttttgacagctggcgggctaacccgccagctgtcgttgttataaccatagtgttgcgttagaaaatggaaagtgcagatatggacggcttga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUCCGCCGAGUUCCUCUUCCACAAAAAUCCAAUUUCACCUUUUGACUGGCAAAAACACAAAGAAACUUGUUCGGUAUUGGUGAGCUGGAUUUGUGCAAUGGUCUCAUUAUCCAGAGUCAGUCGUUUGUACUUUGUUCUUUCCAUUUCAACAUCAAAGCUAUGAACGAUUUUUGACAGCUGGCGGGCUAACCCGCCAGCUGUCGUUGUUAUAACCAUAGUGUUGCGUUAGAAAAUGGAAAGUGCAGAUAUGGACGGCUUGA ........(((..((.....(((((..(((((..((((((.....((((((((..............))).)))))...)))))).))))))))))....)).)))...........(((((((((..((.(((((.((((((((((((((..(((.((((((((((((....(((((((((((((....))))))))))))))))))).....)))))).))).))).).)))))))))).)))))))..)))))).))) (-93.94) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCAAGCCGUCCAUAUCUGCACUUUCCAUUUUCUAACGCAACACUAUGGUUAUAACAACGACAGCUGGCGGGUUAGCCCGCCAGCUGUCAAAAAUCGUUCAUAGCUUUGAUGUUGAAAUGGAAAGAACAAAGUACAAACGACUGACUCUGGAUAAUGAGACCAUUGCACAAAUCCAGCUCACCAAUACCGAACAAGUUUCUUUGUGUUUUUGCCAGUCAAAAGGUGAAAUUGGAUUUUUGUGGAAGAGGAACUCGGCGGAGA ....(((((((...(((...(((((((((((..(((((((..((((((..........(((((((((((((....))))))))))))).........))))))..))).)))))))))))))))..((.(((.((......))))).)).......)))...(..((.((((((((.(((((........(((((....)))))..(((((.....))))))))))..)))))))).))..)....)))...))))..... (-84.71) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 412 117 71 97 48 NC_002950:PG1172|5end_iron-sulfur_cluster_binding_protein,_putative_1251173..1251403_POSITIVE 372 209 166 202 148 NC_002950:PG0146|5end_hypothetical_protein_169115..169345_NEGATIVE 359 200 159 168 138 NC_002950:PG0684|5end_ABC_transporter,_permease_protein,_putative_737648..737878_POSITIVE 355 205 169 149 121 NC_002950:PG0141|5end_spoOJ_protein_164747..164977_NEGATIVE 347 216 175 57 12 NC_002950:PG0362|5end_hypothetical_protein_395301..395531_POSITIVE 347 109 67 214 162 NC_002950:PG0307|5end_NADH-ubiquinone_oxidoreductase_342560..342790_POSITIVE 345 86 43 169 111 NC_002950:PG0801|5end_polyA_polymerase_family_protein_857714..857944_NEGATIVE 340 205 170 37 2 NC_002950:PG0017|5end_sensor_histidine_kinase_18307..18537_POSITIVE 336 228 181 172 118 NC_002950:PG2087|5end_hypothetical_protein_2186990..2187220_POSITIVE 336 230 181 103 57 NC_002950:PG1488|5end_hypothetical_protein_1561747..1561977_POSITIVE 335 216 171 221 183 NC_002950:PG0494|5end_hypothetical_protein_535366..535596_NEGATIVE 333 203 169 186 140 NC_002950:PG2048|5end_hypothetical_protein_2148514..2148744_NEGATIVE 333 220 171 81 38 NC_002950:PG1992|5end_glucose-inhibited_division_protein_A_2081410..2081640_POSITIVE 329 205 165 57 18 NC_002950:PG1939|5end_30S_ribosomal_protein_S10_2025081..2025311_NEGATIVE 328 120 72 97 46 NC_002950:PG2159|5end_protoporphyrinogen_oxidase_2264397..2264627_POSITIVE 328 206 161 48 5 NC_002950:PG1770|5end_hypothetical_protein_1857284..1857514_POSITIVE 328 209 166 204 163 NC_002950:PG0559|5end_chlorohydrolase_family_protein_617496..617726_NEGATIVE 326 207 178 41 5 NC_002950:PG1675|5end_hypothetical_protein_1760182..1760412_POSITIVE 324 200 158 184 130 NC_002950:PG0978|5end_shikimate_5-dehydrogenase_1040033..1040263_POSITIVE 320 203 166 51 8 NC_002950:PG0441|5end_hypothetical_protein_478734..478964_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 359 200 159 141 111 NC_002950:PG0683|3end_ABC_transporter,_permease_protein,_putative_737701..737851_POSITIVE 355 205 169 141 113 NC_002950:PG0142|3end_SpoOJ_regulator_protein_164755..164905_NEGATIVE 336 230 181 75 29 NC_002950:PG1487|3end_hypothetical_protein_1561799..1561949_POSITIVE 329 205 165 41 2 NC_002950:PG1940|3end_elongation_factor_EF-2_2025097..2025247_NEGATIVE 328 120 72 93 42 NC_002950:PG2158|3end_hypothetical_protein_2264473..2264623_POSITIVE 328 128 81 66 2 NC_002950:PG1312|3end_capA_protein,_putative_1391553..1391703_POSITIVE 326 207 178 139 103 NC_002950:PG1675|3end_hypothetical_protein_1760360..1760510_POSITIVE 325 216 176 133 95 NC_002950:PG0361|3end_hypothetical_protein_395481..395631_POSITIVE 321 207 166 118 65 NC_002950:PG1286|3end_ferritin_1365019..1365169_NEGATIVE 321 196 152 144 90 NC_002950:PG0619|3end_alkyl_hydroperoxide_reductase,_F_subunit_672731..672881_POSITIVE 320 203 166 47 4 NC_002950:PG0439|3end_hypothetical_protein_478810..478960_POSITIVE 318 228 181 107 48 NC_002950:PG0803|3end_glucosamine-6-phosphate_deaminase_859727..859877_NEGATIVE 316 200 154 101 59 NC_002950:PG0672|3end_iron_compound_ABC_transporter,_ATP-binding_protein_723840..723990_POSITIVE 314 128 82 54 16 NC_002950:PG0636|3end_MATE_efflux_family_protein_687707..687857_NEGATIVE 309 208 174 58 11 NC_002950:PG0735|3end_aminotransferase,_class_V_785677..785827_POSITIVE 306 238 192 71 20 NC_002950:PG1341|3end_hypothetical_protein_1422665..1422815_POSITIVE 304 205 161 91 41 NC_002950:PG1391|3end_hypothetical_protein_1472961..1473111_POSITIVE 303 204 163 122 62 NC_002950:PG1352|3end_hypothetical_protein_1432737..1432887_NEGATIVE 303 209 161 118 63 NC_002950:PG1296|3end_hypothetical_protein_1375329..1375479_POSITIVE 300 226 182 109 64 NC_002950:PG0334|3end_glycosyl_transferase,_group_2_family_protein_368792..368942_NEGATIVE