Origin IGS: aaaatgaaaaacctgtttggggctggtccccgaatgttttttttatttccaagccattcgtatcgtatctgcacaccaattacatgaatggcgtgcttaattttaatgctctcatttatagctacttatattttcggaaaaaagagctaaaacgaccctcaaactaaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggttttgccctttgtaagctttacaaaatctttatcatagcaaacctttcggaggagtccttctcgagtgtgcgatatgtataggcaggcccttctattctaatatatatcggtagaatcttaatgcctgctatttcagcagagatgtttagcctgtggtcatgatccataggaaaagaagagggtgttgcaagacaaaagttgcaacaccctcttggctttcgtcggaaaggaacagagctttcctatatataaggagtc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gactccttatatataggaaagctctgttcctttccgacgaaagccaagagggtgttgcaacttttgtcttgcaacaccctcttcttttcctatggatcatgaccacaggctaaacatctctgctgaaatagcaggcattaagattctaccgatatatattagaatagaagggcctgcctatacatatcgcacactcgagaaggactcctccgaaaggtttgctatgataaagattttgtaaagcttacaaagggcaaaaccaaagtatatagtttggtttcatcaaagtatatagtttggttcgaccaaagtatatagttcggtttgaccaaactatatagtttagttcaaccaaactatatagtttagtttgagggtcgttttagctcttttttccgaaaatataagtagctataaatgagagcattaaaattaagcacgccattcatgtaattggtgtgcagatacgatacgaatggcttggaaataaaaaaaacattcggggaccagccccaaacaggtttttcatttt Mask Tandem Repeat Region ================================================ aaaatgaaaaacctgtttggggctggtccccgaatgttttttttatttccaagccattcgtatcgtatctgcacaccaattacatgaatggcgtgcttaattttaatgctctcatttatagctacttatattttcggaaaaaagagctaaaacgacccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttgccctttgtaagctttacaaaatctttatcatagcaaacctttcggaggagtccttctcgagtgtgcgatatgtataggcaggcccttctattctaatatatatcggtagaatcttaatgcctgctatttcagcagagatgtttagcctgtggtcatgatccataggaaaagaagagggtgttgcaagacaaaagttgcaacaccctcttggctttcgtcggaaaggaacagagctttcctatatataaggagtc Find is-nt database================================================ Query_seq: PG0711:PG0712|PG0711:PG0712:hypothetical protein:hypothetical protein:-><-:766342..766873 532 aaaatgaaaaacctgtttggggctggtccccgaatgttttttttatttccaagccattcgtatcgtatctgcacaccaattacatgaatggcgtgcttaattttaatgctctcatttatagctacttatattttcggaaaaaagagctaaaacgaccctcaaactaaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggttttgccctttgtaagctttacaaaatctttatcatagcaaacctttcggaggagtccttctcgagtgtgcgatatgtataggcaggcccttctattctaatatatatcggtagaatcttaatgcctgctatttcagcagagatgtttagcctgtggtcatgatccataggaaaagaagagggtgttgcaagacaaaagttgcaacaccctcttggctttcgtcggaaaggaacagagctttcctatatataaggagtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0711:PG0712|PG0711:PG0712:hypothetical protein:hypothetical protein:-><-:766342..766873 532 aaaatgaaaaacctgtttggggctggtccccgaatgttttttttatttccaagccattcgtatcgtatctgcacaccaattacatgaatggcgtgcttaattttaatgctctcatttatagctacttatattttcggaaaaaagagctaaaacgaccctcaaactaaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggttttgccctttgtaagctttacaaaatctttatcatagcaaacctttcggaggagtccttctcgagtgtgcgatatgtataggcaggcccttctattctaatatatatcggtagaatcttaatgcctgctatttcagcagagatgtttagcctgtggtcatgatccataggaaaagaagagggtgttgcaagacaaaagttgcaacaccctcttggctttcgtcggaaaggaacagagctttcctatatataaggagtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0711:PG0712|PG0711:PG0712:hypothetical protein:hypothetical protein:-><-:766342..766873 532 aaaatgaaaaacctgtttggggctggtccccgaatgttttttttatttccaagccattcgtatcgtatctgcacaccaattacatgaatggcgtgcttaattttaatgctctcatttatagctacttatattttcggaaaaaagagctaaaacgaccctcaaactaaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggttttgccctttgtaagctttacaaaatctttatcatagcaaacctttcggaggagtccttctcgagtgtgcgatatgtataggcaggcccttctattctaatatatatcggtagaatcttaatgcctgctatttcagcagagatgtttagcctgtggtcatgatccataggaaaagaagagggtgttgcaagacaaaagttgcaacaccctcttggctttcgtcggaaaggaacagagctttcctatatataaggagtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaatgaaaaacctgtttggggctggtccccgaatgttttttttatttccaagccattcgtatcgtatctgcacaccaattacatgaatggcgtgcttaattttaatgctctcatttatagctacttatattttcggaaaaaagagctaaaacgaccctcaaactaaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggttttgccctttgtaagctttacaaaatctttatcatagcaaacctttcggaggagtccttctcgagtgtgcgatatgtataggcaggcccttctattctaatatatatcggtagaatcttaatgcctgctatttcagcagagatgtttagcctgtggtcatgatccataggaaaagaagagggtgttgcaagacaaaagttgcaacaccctcttggctttcgtcggaaaggaacagagctttcctatatataaggagtc Predict ORF larger than 30AA ================================================ Protein_Len: 40 Strand: + Start: 45 End: 164 ............................................ M S K P F V S Y L H T N Y M N G V L N F N A L I Y S Y L Y F R K K E L K R P S N ................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 36 Strand: + Start: 178 End: 285 ................................................................................................................................................................................. M V E L N Y I V W S N R T I Y F G R T K L Y T L M K P N Y I L W F C P L ....................................................................................................................................................................................................................................................... Protein_Len: 36 Strand: + Start: 201 End: 308 ........................................................................................................................................................................................................ M V K P N Y I L W S N Q T I Y F D E T K L Y T L V L P F V S F T K S L S ................................................................................................................................................................................................................................ Protein_Len: 30 Strand: + Start: 263 End: 352 ...................................................................................................................................................................................................................................................................... M Y F G F A L C K L Y K I F I I A N L S E E S F S S V R Y V .................................................................................................................................................................................... Protein_Len: 35 Strand: + Start: 427 End: 531 .......................................................................................................................................................................................................................................................................................................................................................................................................................................... M V M I H R K R R G C C K T K V A T P S W L S S E R N R A F L Y I R S . Protein_Len: 34 Strand: - Start: 423 End: 524 ...................................................................................................................................................................................................................................................................................................................................................................................................................................... G T T M I W L F L L P H Q L V F T A V G E Q S E D S L F L A K R Y M ........ Protein_Len: 49 Strand: - Start: 295 End: 441 ...................................................................................................................................................................................................................................................................................................... L I K I M A F R E S S D K E L T R Y T Y A P G E I R I Y I P L I K I G A I E A S I N L R H D H D M ........................................................................................... Protein_Len: 44 Strand: - Start: 218 End: 349 ......................................................................................................................................................................................................................... I S Q D F W V I Y K S S V L S Y V K T K G K T L K V F D K D Y C V K R L L G E R T H S M ....................................................................................................................................................................................... Protein_Len: 39 Strand: - Start: 3 End: 119 .. I F F R N P A P G R I N K K N G L W E Y R I Q V G I V H I A H K I K I S E N M ............................................................................................................................................................................................................................................................................................................................................................................................................................. aaaatgaaaaacctgtttggggctggtccccgaatgttttttttatttccaagccattcgtatcgtatctgcacaccaattacatgaatggcgtgcttaattttaatgctctcatttatagctacttatattttcggaaaaaagagctaaaacgaccctcaaactaaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggttttgccctttgtaagctttacaaaatctttatcatagcaaacctttcggaggagtccttctcgagtgtgcgatatgtataggcaggcccttctattctaatatatatcggtagaatcttaatgcctgctatttcagcagagatgtttagcctgtggtcatgatccataggaaaagaagagggtgttgcaagacaaaagttgcaacaccctcttggctttcgtcggaaaggaacagagctttcctatatataaggagtc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaatgaaaaacctgtttggggctggtccccgaatgttttttttatttccaagccattcgtatcgtatctgcacaccaattacatgaatggcgtgcttaattttaatgctctcatttatagctacttatattttcggaaaaaagagctaaaacgaccctcaaactaaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggttttgccctttgtaagctttacaaaatctttatcatagcaaacctttcggaggagtccttctcgagtgtgcgatatgtataggcaggcccttctattctaatatatatcggtagaatcttaatgcctgctatttcagcagagatgtttagcctgtggtcatgatccataggaaaagaagagggtgttgcaagacaaaagttgcaacaccctcttggctttcgtcggaaaggaacagagctttcctatatataaggagtc Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 95 HP_score: -12.9 Tail_Score: -3.60431 Start: 164 End: 204 Full_Region: tatagttcggtttga ccaaactatatagtttagt tca accaaactatatagtttag tttgagggtcgtttt ...................................................................................................................................................................ccaaactatatagtttagttcaaccaaactatatagtttag........................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 100 HP_score: -13.6 Tail_Score: -3.72576 Start: 169 End: 199 Full_Region: GACCCTCAAACTAAA CTATATAGTTTGGT TGA ACTAAACTATATAG TTTGGTCAAACCGAA ........................................................................................................................................................................CTATATAGTTTGGTTGAACTAAACTATATAG............................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 81 HP_score: -9.9 Tail_Score: -3.72576 Start: 169 End: 222 Full_Region: GACCCTCAAACTAAA CTATATAGTTTGGTTGAACTAAACT ATAT AGTTTGGTCAAACCGAACTATATAC TTTGGTCGAACCAAA ........................................................................................................................................................................CTATATAGTTTGGTTGAACTAAACTATATAGTTTGGTCAAACCGAACTATATAC...................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 90 HP_score: -11.4 Tail_Score: -4.06502 Start: 169 End: 199 Full_Region: ttcggtttgaccaaa ctatatagtttagt tca accaaactatatag tttagtttgagggtc ........................................................................................................................................................................ctatatagtttagttcaaccaaactatatag............................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 93 HP_score: -13.4 Tail_Score: -2.83403 Start: 170 End: 221 Full_Region: ACCCTCAAACTAAAC TATATAGTTTGGTTGAACTAAACT ATAT AGTTTGGTCAAACCGAACTATATA CTTTGGTCGAACCAA .........................................................................................................................................................................TATATAGTTTGGTTGAACTAAACTATATAGTTTGGTCAAACCGAACTATATA....................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 79 HP_score: -11.2 Tail_Score: -3.03419 Start: 170 End: 221 Full_Region: ttggttcgaccaaag tatatagttcggtttgaccaaact atat agtttagttcaaccaaactatata gtttagtttgagggt .........................................................................................................................................................................tatatagttcggtttgaccaaactatatagtttagttcaaccaaactatata....................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 74 HP_score: -9.4 Tail_Score: -3.0693 Start: 172 End: 219 Full_Region: ggttcgaccaaagta tatagttcggtttgaccaaact atat agtttagttcaaccaaactata tagtttagtttgagg ...........................................................................................................................................................................tatagttcggtttgaccaaactatatagtttagttcaaccaaactata......................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 82 HP_score: -11.6 Tail_Score: -2.96695 Start: 172 End: 219 Full_Region: CCTCAAACTAAACTA TATAGTTTGGTTGAACTAAACT ATAT AGTTTGGTCAAACCGAACTATA TACTTTGGTCGAACC ...........................................................................................................................................................................TATAGTTTGGTTGAACTAAACTATATAGTTTGGTCAAACCGAACTATA......................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 72 HP_score: -7.0 Tail_Score: -3.90112 Start: 192 End: 222 Full_Region: tttggttcgaccaaa gtatatagttcggt ttg accaaactatatag tttagttcaaccaaa ...............................................................................................................................................................................................gtatatagttcggtttgaccaaactatatag...................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 76 HP_score: -7.1 Tail_Score: -4.26189 Start: 233 End: 273 Full_Region: TATACTTTGGTCGAA CCAAACTATATACTTTGAT GAA ACCAAACTATATACTTTGG TTTTGCCCTTTGTAA ........................................................................................................................................................................................................................................CCAAACTATATACTTTGATGAAACCAAACTATATACTTTGG................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -13.8 Tail_Score: -4.3586 Start: 449 End: 488 Full_Region: ctttccgacgaaagc caagagggtgttgcaa cttttgtc ttgcaacaccctcttc ttttcctatggatca ................................................................................................................................................................................................................................................................................................................................................................................................................................................................caagagggtgttgcaacttttgtcttgcaacaccctcttc............................................ TransTerm Strand: + Conf: 72 HP_score: -7.6 Tail_Score: -3.72576 Start: 449 End: 490 Full_Region: TGATCCATAGGAAAA GAAGAGGGTGTTGCAA GACAAAAG TTGCAACACCCTCTTGGC TTTCGTCGGAAAGGA ................................................................................................................................................................................................................................................................................................................................................................................................................................................................GAAGAGGGTGTTGCAAGACAAAAGTTGCAACACCCTCTTGGC.......................................... TransTerm Strand: - Conf: 100 HP_score: -15.5 Tail_Score: -4.29302 Start: 452 End: 485 Full_Region: tccgacgaaagccaa gagggtgttgcaa cttttgtc ttgcaacaccctc ttcttttcctatgga ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................gagggtgttgcaacttttgtcttgcaacaccctc............................................... Find igs database================================================ Query_seq: PG0711:PG0712|PG0711:PG0712:hypothetical protein:hypothetical protein:-><-:766342..766873 532 aaaatgaaaaacctgtttggggctggtccccgaatgttttttttatttccaagccattcgtatcgtatctgcacaccaattacatgaatggcgtgcttaattttaatgctctcatttatagctacttatattttcggaaaaaagagctaaaacgaccctcaaactaaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggttttgccctttgtaagctttacaaaatctttatcatagcaaacctttcggaggagtccttctcgagtgtgcgatatgtataggcaggcccttctattctaatatatatcggtagaatcttaatgcctgctatttcagcagagatgtttagcctgtggtcatgatccataggaaaagaagagggtgttgcaagacaaaagttgcaacaccctcttggctttcgtcggaaaggaacagagctttcctatatataaggagtc Intra-Species Hit: Count: 4 Min: 1 Max: 532 Len: 532 Subject: NC_002950_PG0711_PG0712|hypothetical protein:hypothetical protein|POSITIVE:NEGATIVE|[766342,766873]|532 HSP 1 e-value: 0.0 bit: 1007.0 Len: 532 Query Start:1 Query End:532 Subject Strand: POSITIVE Subject Start: 1 Subject End: 532 aaaatgaaaaacctgtttggggctggtccccgaatgnnnnnnnnatttccaagccattcgtatcgtatctgcacaccaattacatgaatggcgtgcttaattttaatgctctcatttatagctacttatattttcggaaaaaagagctaaaacgaccctcaaactaaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggttttgccctttgtaagctttacaaaatctttatcatagcaaacctttcggaggagtccttctcgagtgtgcgatatgtataggcaggcccttctattctaatatatatcggtagaatcttaatgcctgctatttcagcagagatgtttagcctgtggtcatgatccataggaaaagaagagggtgttgcaagacaaaagttgcaacaccctcttggctttcgtcggaaaggaacagagctttcctatatataaggagtc aaaatgaaaaacctgtttggggctggtccccgaatgttttttttatttccaagccattcgtatcgtatctgcacaccaattacatgaatggcgtgcttaattttaatgctctcatttatagctacttatattttcggaaaaaagagctaaaacgaccctcaaactaaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggttttgccctttgtaagctttacaaaatctttatcatagcaaacctttcggaggagtccttctcgagtgtgcgatatgtataggcaggcccttctattctaatatatatcggtagaatcttaatgcctgctatttcagcagagatgtttagcctgtggtcatgatccataggaaaagaagagggtgttgcaagacaaaagttgcaacaccctcttggctttcgtcggaaaggaacagagctttcctatatataaggagtc Subject: NC_002950_PG0453_PG0456|hypothetical protein:PHP N-terminal domain protein|POSITIVE:POSITIVE|[496731,497854]|1124 HSP 1 e-value: 1.0E-43 bit: 178.0 Len: 122 Query Start:160 Query End:281 Subject Strand: POSITIVE Subject Start: 71 Subject End: 192 ...............................................................................................................................................................caaactaaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggttttgccc........................................................................................................................................................................................................................................................... ...............................................................................................................................................................caaactaaactatatagtttggttcaactaaactatatactttggtccgactaaactatatactttggtcgaaccaaactatatactttgataaagccaaactatatactttggttttgccc........................................................................................................................................................................................................................................................... HSP 2 e-value: 5.0E-6 bit: 54.0 Len: 75 Query Start:170 Query End:244 Subject Strand: NEGATIVE Subject Start: 81 Subject End: 155 .........................................................................................................................................................................tatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatata................................................................................................................................................................................................................................................................................................ .........................................................................................................................................................................tatatagtttggttcgaccaaagtatatagtttagtcggaccaaagtatatagtttagttgaaccaaactatata................................................................................................................................................................................................................................................................................................ Subject: NC_002950_PG0281_PG0282|ABC transporter, permease protein, putative:ABC transporter, ATP-binding protein|NEGATIVE:NEGATIVE|[316114,316333]|220 HSP 1 e-value: 3.0E-23 bit: 111.0 Len: 84 Query Start:166 Query End:249 Subject Strand: POSITIVE Subject Start: 43 Subject End: 126 .....................................................................................................................................................................aaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttg........................................................................................................................................................................................................................................................................................... .....................................................................................................................................................................aaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttg........................................................................................................................................................................................................................................................................................... HSP 2 e-value: 9.0E-14 bit: 79.8 Len: 88 Query Start:189 Query End:276 Subject Strand: POSITIVE Subject Start: 43 Subject End: 130 ............................................................................................................................................................................................aaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggttt................................................................................................................................................................................................................................................................ ............................................................................................................................................................................................aaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggttt................................................................................................................................................................................................................................................................ HSP 3 e-value: 2.0E-11 bit: 71.9 Len: 92 Query Start:160 Query End:251 Subject Strand: NEGATIVE Subject Start: 40 Subject End: 131 ...............................................................................................................................................................caaactaaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgat......................................................................................................................................................................................................................................................................................... ...............................................................................................................................................................caaaccaaagtatatagtttggttcaaccaaagtatatagttcggtcagaccaaactatatagtttagtcagaccaaagtatatagtttgat......................................................................................................................................................................................................................................................................................... HSP 4 e-value: 8.0E-8 bit: 60.0 Len: 66 Query Start:163 Query End:228 Subject Strand: POSITIVE Subject Start: 63 Subject End: 128 ..................................................................................................................................................................actaaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggt................................................................................................................................................................................................................................................................................................................ ..................................................................................................................................................................actaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggt................................................................................................................................................................................................................................................................................................................ HSP 5 e-value: 5.0E-6 bit: 54.0 Len: 79 Query Start:189 Query End:267 Subject Strand: NEGATIVE Subject Start: 70 Subject End: 148 ............................................................................................................................................................................................aaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatata......................................................................................................................................................................................................................................................................... ............................................................................................................................................................................................aaactatatagtttgaccaaaccaaagtatatagtttggttcaaccaaagtatatagttcggtcagaccaaactatata......................................................................................................................................................................................................................................................................... Subject: NC_002950_PG0555_PG0556|DNA-binding protein, histone-like family:hypothetical protein|NEGATIVE:POSITIVE|[612325,613417]|1093 HSP 1 e-value: 1.0E-22 bit: 109.0 Len: 107 Query Start:166 Query End:272 Subject Strand: NEGATIVE Subject Start: 226 Subject End: 332 .....................................................................................................................................................................aaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttg.................................................................................................................................................................................................................................................................... .....................................................................................................................................................................aaactatatactttggcaaaactaaactatatagtttggtgagactaaactatatactttggttcaaccaaactatatactttggtgagaccaaactatatagtttg.................................................................................................................................................................................................................................................................... HSP 2 e-value: 6.0E-18 bit: 93.7 Len: 91 Query Start:161 Query End:251 Subject Strand: NEGATIVE Subject Start: 224 Subject End: 314 ................................................................................................................................................................aaactaaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgat......................................................................................................................................................................................................................................................................................... ................................................................................................................................................................aaactaaactatatagtttggtgagactaaactatatactttggttcaaccaaactatatactttggtgagaccaaactatatagtttgat......................................................................................................................................................................................................................................................................................... HSP 3 e-value: 3.0E-10 bit: 67.9 Len: 86 Query Start:189 Query End:274 Subject Strand: NEGATIVE Subject Start: 247 Subject End: 332 ............................................................................................................................................................................................aaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggt.................................................................................................................................................................................................................................................................. ............................................................................................................................................................................................aaactatatactttggcaaaactaaactatatagtttggtgagactaaactatatactttggttcaaccaaactatatactttggt.................................................................................................................................................................................................................................................................. HSP 4 e-value: 5.0E-9 bit: 63.9 Len: 92 Query Start:189 Query End:280 Subject Strand: POSITIVE Subject Start: 227 Subject End: 318 ............................................................................................................................................................................................aaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggttttgcc............................................................................................................................................................................................................................................................ ............................................................................................................................................................................................aaactatatagtttggtctcaccaaagtatatagtttggttgaaccaaagtatatagtttagtctcaccaaactatatagtttagttttgcc............................................................................................................................................................................................................................................................ HSP 5 e-value: 2.0E-8 bit: 61.9 Len: 63 Query Start:213 Query End:275 Subject Strand: NEGATIVE Subject Start: 269 Subject End: 331 ....................................................................................................................................................................................................................aactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggtt................................................................................................................................................................................................................................................................. ....................................................................................................................................................................................................................aactatatactttggcaaaactaaactatatagtttggtgagactaaactatatactttggtt................................................................................................................................................................................................................................................................. HSP 6 e-value: 1.0E-6 bit: 56.0 Len: 52 Query Start:170 Query End:221 Subject Strand: POSITIVE Subject Start: 254 Subject End: 305 .........................................................................................................................................................................tatatagtttggttgaactaaactatatagtttggtcaaaccgaactatata....................................................................................................................................................................................................................................................................................................................... .........................................................................................................................................................................tatatagtttggttgaaccaaagtatatagtttagtctcaccaaactatata....................................................................................................................................................................................................................................................................................................................... HSP 7 e-value: 5.0E-6 bit: 54.0 Len: 79 Query Start:166 Query End:244 Subject Strand: POSITIVE Subject Start: 227 Subject End: 305 .....................................................................................................................................................................aaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatata................................................................................................................................................................................................................................................................................................ .....................................................................................................................................................................aaactatatagtttggtctcaccaaagtatatagtttggttgaaccaaagtatatagtttagtctcaccaaactatata................................................................................................................................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAUGAAAAACCUGUUUGGGGCUGGUCCCCGAAUGUUUUUUUUAUUUCCAAGCCAUUCGUAUCGUAUCUGCACACCAAUUACAUGAAUGGCGUGCUUAAUUUUAAUGCUCUCAUUUAUAGCUACUUAUAUUUUCGGAAAAAAGAGCUAAAACGACCCUCAAACUAAACUAUAUAGUUUGGUUGAACUAAACUAUAUAGUUUGGUCAAACCGAACUAUAUACUUUGGUCGAACCAAACUAUAUACUUUGAUGAAACCAAACUAUAUACUUUGGUUUUGCCCUUUGUAAGCUUUACAAAAUCUUUAUCAUAGCAAACCUUUCGGAGGAGUCCUUCUCGAGUGUGCGAUAUGUAUAGGCAGGCCCUUCUAUUCUAAUAUAUAUCGGUAGAAUCUUAAUGCCUGCUAUUUCAGCAGAGAUGUUUAGCCUGUGGUCAUGAUCCAUAGGAAAAGAAGAGGGUGUUGCAAGACAAAAGUUGCAACACCCUCUUGGCUUUCGUCGGAAAGGAACAGAGCUUUCCUAUAUAUAAGGAGUC ..(((((((((..(((((((((.....)))))))))..)))))))))(((.((((...((((((((((..((((.(((.((....)).))).))))...........((((.......(((((.(((..............))))))))....(((((((.........((((((((((((((..((((((.((((((((((((.....)))))))))))).))))))..))))))))))))))((.(((((((((((((........)))))))))....((((((.....))))))......)))).))...........)))).)))......))))))))))))))....(((((((......((((((...........))))))......)))))))(((((....)))))......(((((((.......))))))).....(((((((((((((((........)))))))))))))))))))......((((((........)))))).........)))... (-167.24) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GACUCCUUAUAUAUAGGAAAGCUCUGUUCCUUUCCGACGAAAGCCAAGAGGGUGUUGCAACUUUUGUCUUGCAACACCCUCUUCUUUUCCUAUGGAUCAUGACCACAGGCUAAACAUCUCUGCUGAAAUAGCAGGCAUUAAGAUUCUACCGAUAUAUAUUAGAAUAGAAGGGCCUGCCUAUACAUAUCGCACACUCGAGAAGGACUCCUCCGAAAGGUUUGCUAUGAUAAAGAUUUUGUAAAGCUUACAAAGGGCAAAACCAAAGUAUAUAGUUUGGUUUCAUCAAAGUAUAUAGUUUGGUUCGACCAAAGUAUAUAGUUCGGUUUGACCAAACUAUAUAGUUUAGUUCAACCAAACUAUAUAGUUUAGUUUGAGGGUCGUUUUAGCUCUUUUUUCCGAAAAUAUAAGUAGCUAUAAAUGAGAGCAUUAAAAUUAAGCACGCCAUUCAUGUAAUUGGUGUGCAGAUACGAUACGAAUGGCUUGGAAAUAAAAAAAACAUUCGGGGACCAGCCCCAAACAGGUUUUUCAUUUU ...............(((((((.(((((..((((((.....((((.((((((((((((((........))))))))))))))....(((.(((.((((..((((.((((((((((.(((((((.((.(((((((((......((((((...........))))))......))))).)))).....))))).....)))).(((....)))....((((((((..........(((((((.....)))))))))).)))))....((((((((((((((..((.(((.((((((((((((((.((((.((.(....).)).)))).)))))))))))))).))).))..))))))))))))))))))))))))..))))......((((((............................))))))...........((((((((..........)))))))).))).).))).))).))))))))))..............((((.....)))).))))))))))))..... (-166.39) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 666 240 191 208 159 NC_002950:PG0281|5end_ABC_transporter,_permease_protein,_putative_316023..316253_NEGATIVE 451 360 317 53 5 NC_002950:PG0005|5end_hypothetical_protein_4951..5181_NEGATIVE 420 504 461 121 88 NC_002950:PG1763|5end_ribonuclease_III_1851712..1851942_NEGATIVE 394 362 321 218 177 NC_002950:PG1693|5end_HesA/MoeB/ThiF_family_protein_1777919..1778149_NEGATIVE 386 365 321 222 174 NC_002950:PG0657|5end_Maf-like_protein_707782..708012_NEGATIVE 380 360 312 59 2 NC_002950:PG2170|5end_sugar_transporter_2277974..2278204_NEGATIVE 379 499 451 126 77 NC_002950:PG0470|5end_hypothetical_protein_511883..512113_NEGATIVE 359 520 472 53 6 NC_002950:PG0293|5end_secretion_activator_protein,_putative_328671..328901_NEGATIVE 348 339 301 228 193 NC_002950:PG1853|5end_DNA_polymerase_III,_beta_subunit_1953119..1953349_NEGATIVE 338 365 321 180 135 NC_002950:PG1020|5end_hypothetical_protein_1080568..1080798_POSITIVE 331 359 321 153 115 NC_002950:PG1959|5end_ribosomal_protein_L33_2048405..2048635_NEGATIVE 328 367 327 63 19 NC_002950:PG0062|5end_TPR_domain_protein_73688..73918_POSITIVE 327 490 442 103 45 NC_002950:PG2098|5end_hypothetical_protein_2206635..2206865_NEGATIVE 327 490 447 46 2 NC_002950:PG1596|5end_isoleucyl-tRNA_synthetase,_putative_1674543..1674773_POSITIVE 327 445 401 198 139 NC_002950:PG0669|5end_heme-binding_protein_FetB_719753..719983_POSITIVE 326 499 451 204 150 NC_002950:PG2109|5end_thiamine-phosphate_pyrophosphorylase_2219452..2219682_NEGATIVE 326 60 12 188 141 NC_002950:PG0148|5end_sigma-54-dependent_transcriptional_regulator_170907..171137_NEGATIVE 325 357 315 207 158 NC_002950:PG1683|5end_hypothetical_protein_1769644..1769874_POSITIVE 325 487 443 176 122 NC_002950:PG1281|5end_hypothetical_protein_1358848..1359078_POSITIVE 321 439 394 72 24 NC_002950:PG0506|5end_arginine-specific_cysteine_proteinase_545335..545565_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 569 497 451 76 25 NC_002950:PG1004|3end_prolyl_oligopeptidase_family_protein_1060433..1060583_NEGATIVE 420 504 461 37 4 NC_002950:PG1764|3end_3-oxoacyl-(acyl-carrier-protein)_synthase_II_1851796..1851946_NEGATIVE 334 489 442 57 8 NC_002950:PG0553|3end_extracellular_protease,_putative_608367..608517_NEGATIVE 331 359 321 134 96 NC_002950:PG1960|3end_ribosomal_protein_L28_2048424..2048574_NEGATIVE 328 366 321 107 29 NC_002950:PG0443|3end_hemagglutinin-related_protein_483147..483297_POSITIVE 328 367 327 58 14 NC_002950:PG0061|3end_yngK_protein_73763..73913_POSITIVE 327 490 442 89 31 NC_002950:PG2099|3end_ATP-dependent_RNA_helicase,_DEAD/DEAH_box_family_2206649..2206799_NEGATIVE 321 439 394 54 6 NC_002950:PG0505|3end_hypothetical_protein_545397..545547_POSITIVE 319 497 451 66 20 NC_002950:PG1414|3end_hypothetical_protein_1499704..1499854_POSITIVE 319 360 311 79 31 NC_002950:PG0656|3end_50S_ribosomal_protein_L34_706945..707095_NEGATIVE 315 368 321 93 42 NC_002950:PG0524|3end_hypothetical_protein_568963..569113_POSITIVE 312 489 442 75 16 NC_002950:PG2117|3end_30S_ribosomal_protein_S16_2225235..2225385_NEGATIVE 312 438 391 98 38 NC_002950:PG1875|3end_hemolysin_1969647..1969797_NEGATIVE 310 366 321 138 77 NC_002950:PG0129|3end_mannosyltransferase_151789..151939_POSITIVE 308 370 322 89 38 NC_002950:PG2172|3end_hypothetical_protein_2279191..2279341_NEGATIVE 308 367 321 140 84 NC_002950:PG0677|3end_saccharopine_dehydrogenase_728926..729076_POSITIVE 307 362 321 138 96 NC_002950:PG1538|3end_undecaprenol_kinase,_putative_1618553..1618703_POSITIVE 307 92 53 150 110 NC_002950:PG1218|3end_hypothetical_protein_1296475..1296625_POSITIVE 307 500 451 66 12 NC_002950:PG0332|3end_transcription_termination_factor_Rho_367675..367825_POSITIVE 306 506 461 82 17 NC_002950:PG1786|3end_hypothetical_protein_1875322..1875472_POSITIVE