Origin IGS: tttcttactatgctgtatgctttatcgtatggctgcattttgctgaggccatacgctaaaaacatttcaaaggtatgtatttgtttggataagcttttccctatcatatattatcgctgctcgt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 acgagcagcgataatatatgatagggaaaagcttatccaaacaaatacatacctttgaaatgtttttagcgtatggcctcagcaaaatgcagccatacgataaagcatacagcatagtaagaaa Mask Tandem Repeat Region ================================================ tttcttactatgctgtatgctttatcgtatggctgcattttgctgaggccatacgctaaaaacatttcaaaggtatgtatttgtttggataagcttttccctatcatatattatcgctgctcgt Find is-nt database================================================ Query_seq: PG0699:PG0700|PG0699:PG0700:maltodextrin phosphorylase:hypothetical protein:<-<-:756049..756172 124 tttcttactatgctgtatgctttatcgtatggctgcattttgctgaggccatacgctaaaaacatttcaaaggtatgtatttgtttggataagcttttccctatcatatattatcgctgctcgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0699:PG0700|PG0699:PG0700:maltodextrin phosphorylase:hypothetical protein:<-<-:756049..756172 124 tttcttactatgctgtatgctttatcgtatggctgcattttgctgaggccatacgctaaaaacatttcaaaggtatgtatttgtttggataagcttttccctatcatatattatcgctgctcgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0699:PG0700|PG0699:PG0700:maltodextrin phosphorylase:hypothetical protein:<-<-:756049..756172 124 tttcttactatgctgtatgctttatcgtatggctgcattttgctgaggccatacgctaaaaacatttcaaaggtatgtatttgtttggataagcttttccctatcatatattatcgctgctcgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttcttactatgctgtatgctttatcgtatggctgcattttgctgaggccatacgctaaaaacatttcaaaggtatgtatttgtttggataagcttttccctatcatatattatcgctgctcgt Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 10 End: 123 ......... M L Y A L S Y G C I L L R P Y A K N I S K V C I C L D K L F P I I Y Y R C S . Protein_Len: 35 Strand: - Start: 3 End: 107 .. R V I S Y A K D Y P Q M K S L G Y A L F M E F T H I Q K S L S K G I M ................. tttcttactatgctgtatgctttatcgtatggctgcattttgctgaggccatacgctaaaaacatttcaaaggtatgtatttgtttggataagcttttccctatcatatattatcgctgctcgt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttcttactatgctgtatgctttatcgtatggctgcattttgctgaggccatacgctaaaaacatttcaaaggtatgtatttgtttggataagcttttccctatcatatattatcgctgctcgt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 47 HP_score: -2.4 Tail_Score: -3.57119 Start: 18 End: 64 Full_Region: TCTTACTATGCTGTA TGCTTTATCGTATGGCTGCA TTTTGC TGAGGCCATACGCTAAAAACA TTTCAAAGGTATGTA .................TGCTTTATCGTATGGCTGCATTTTGCTGAGGCCATACGCTAAAAACA............................................................ Find igs database================================================ Query_seq: PG0699:PG0700|PG0699:PG0700:maltodextrin phosphorylase:hypothetical protein:<-<-:756049..756172 124 tttcttactatgctgtatgctttatcgtatggctgcattttgctgaggccatacgctaaaaacatttcaaaggtatgtatttgtttggataagcttttccctatcatatattatcgctgctcgt Intra-Species Hit: Count: 1 Min: 1 Max: 124 Len: 124 Subject: NC_002950_PG0699_PG0700|maltodextrin phosphorylase:hypothetical protein|NEGATIVE:NEGATIVE|[756049,756172]|124 HSP 1 e-value: 1.0E-64 bit: 246.0 Len: 124 Query Start:1 Query End:124 Subject Strand: POSITIVE Subject Start: 1 Subject End: 124 tttcttactatgctgtatgctttatcgtatggctgcattttgctgaggccatacgctaaaaacatttcaaaggtatgtatttgtttggataagcttttccctatcatatattatcgctgctcgt tttcttactatgctgtatgctttatcgtatggctgcattttgctgaggccatacgctaaaaacatttcaaaggtatgtatttgtttggataagcttttccctatcatatattatcgctgctcgt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUCUUACUAUGCUGUAUGCUUUAUCGUAUGGCUGCAUUUUGCUGAGGCCAUACGCUAAAAACAUUUCAAAGGUAUGUAUUUGUUUGGAUAAGCUUUUCCCUAUCAUAUAUUAUCGCUGCUCGU ...........((.......((((.(((((((((((.....))...))))))))).))))...........((((.((((.((...(((........)))....)).))))))))...)).... (-23.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACGAGCAGCGAUAAUAUAUGAUAGGGAAAAGCUUAUCCAAACAAAUACAUACCUUUGAAAUGUUUUUAGCGUAUGGCCUCAGCAAAAUGCAGCCAUACGAUAAAGCAUACAGCAUAGUAAGAAA .......((..........((((((......))))))......................(((((((...((((((((..((......))..))))))))..)))))))...))........... (-26.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 943 60 11 151 102 NC_002950:PG0699|5end_maltodextrin_phosphorylase_755958..756188_NEGATIVE 331 60 13 53 1 NC_002950:PG0362|5end_hypothetical_protein_395301..395531_POSITIVE 310 50 8 68 16 NC_002950:PG0160|5end_hypothetical_protein_184085..184315_NEGATIVE 302 54 12 164 124 NC_002950:PG0635|5end_ribosomal_protein_L11_methyltransferase_687773..688003_NEGATIVE 302 50 2 231 171 NC_002950:PG0386|5end_site-specific_recombinase,_phage_integrase_family/ribosomal_subunit_interface_protein_419047..419277_POSITIVE 302 57 15 190 145 NC_002950:PG0117|5end_polysaccharide_transport_protein,_putative_139667..139897_POSITIVE 299 57 16 39 1 NC_002950:PG2015|5end_CRISPR-associated_protein_Cas4_2106014..2106244_NEGATIVE 297 55 11 131 94 NC_002950:PG1848|5end_RNA_methyltransferase,_TrmH_family_1947382..1947612_NEGATIVE 296 48 6 182 150 NC_002950:PG1639|5end_hypothetical_protein_1719919..1720149_NEGATIVE 290 57 12 99 42 NC_002950:PG0606|5end_hypothetical_protein_662558..662788_NEGATIVE 290 52 11 173 129 NC_002950:PG0254|5end_transcription_elongation_factor_NusA_285112..285342_POSITIVE 290 51 18 52 15 NC_002950:PG0018|5end_hypothetical_protein_19648..19878_POSITIVE 289 58 13 205 156 NC_002950:PG1306|5end_metallo-beta-lactamase_family_protein_1387187..1387417_NEGATIVE 288 50 12 210 170 NC_002950:PG0073|5end_orotidine_5'-monophosphate_decarboxylase_88070..88300_NEGATIVE 284 57 15 57 5 NC_002950:PG1497|5end_DNA-binding_protein,_histone-like_family_1574085..1574315_POSITIVE 283 64 22 71 30 NC_002950:PG1835|5end_lipoprotein,_putative_1927073..1927303_POSITIVE 282 56 12 94 39 NC_002950:PG1242|5end_replicative_DNA_helicase_1317435..1317665_POSITIVE 281 58 11 71 18 NC_002950:PG0302|5end_hypothetical_protein_338219..338449_POSITIVE 279 57 12 62 17 NC_002950:PG1954|5end_NAD_dependent_epimerase/reductase-related_protein_2044513..2044743_POSITIVE 279 57 13 61 8 NC_002950:PG1741|5end_aspartate_ammonia-lyase_1827283..1827513_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 345 56 11 131 81 NC_002950:PG1027|3end_hypothetical_protein_1089197..1089347_NEGATIVE 314 59 11 129 82 NC_002950:PG0334|3end_glycosyl_transferase,_group_2_family_protein_368792..368942_NEGATIVE 308 56 11 89 39 NC_002950:PG1779|3end_hypothetical_protein_1868498..1868648_POSITIVE 304 68 31 110 56 NC_002950:PG0572|3end_hypothetical_protein_627434..627584_NEGATIVE 299 50 8 142 104 NC_002950:PG1285|3end_glucosamine-6-phosphate_deaminase_1364941..1365091_POSITIVE 297 55 11 134 97 NC_002950:PG1849|3end_DNA_repair_protein_RecN_1947379..1947529_NEGATIVE 296 58 19 151 107 NC_002950:PG0361|3end_hypothetical_protein_395481..395631_POSITIVE 295 57 11 143 98 NC_002950:PG0344|3end_purple_acid_phosphatase_378543..378693_NEGATIVE 292 56 11 121 65 NC_002950:PG1410|3end_hypothetical_protein_1493436..1493586_POSITIVE 290 52 11 126 82 NC_002950:PG0253|3end_hypothetical_protein_285145..285295_POSITIVE 290 51 18 48 11 NC_002950:PG0017|3end_sensor_histidine_kinase_19724..19874_POSITIVE 288 57 11 42 2 NC_002950:PG0754|3end_DNA_topoisomerase_I_803389..803539_POSITIVE 282 56 12 59 4 NC_002950:PG1241|3end_GTP-binding_protein_LepA_1317480..1317630_POSITIVE 279 57 12 58 13 NC_002950:PG1953|3end_YitT_family_protein_2044589..2044739_POSITIVE 277 49 18 80 51 NC_002950:PG0272|3end_CBS_domain_protein_305738..305888_POSITIVE 276 49 10 48 7 NC_002950:PG0530|3end_carbamoyl-phosphate_synthase,_large_subunit_579036..579186_POSITIVE 275 58 11 130 84 NC_002950:PG0569|3end_hypothetical_protein_624697..624847_NEGATIVE 272 49 1 57 3 NC_002950:PG2213|3end_nitrite_reductase-related_protein_2324848..2324998_NEGATIVE 272 51 30 47 26 NC_002950:PG0377|3end_30S_ribosomal_protein_S2_407845..407995_POSITIVE 270 56 13 119 77 NC_002950:PG0752|3end_uracil_phosphoribosyltransferase_798397..798547_POSITIVE