Origin IGS: aataagccggaatttttcctccggacaataagccggaagggtgtgttgaaatcgcaatcaaaagacgattttggcacacccttttcgtatgtggtaaactctatggcatagggcaaccacgagaaggctgcctgaggattcctccatagggactcttggagaatatcctttggagggcagtatcaagggaaaagacttcatagtataaagggtgggggctttatagtataaagggtagagggtttacggtataaagtatggaggctttatagtataaagggtagagggtttacagtataaagtatggaggctttatggtacaaaggacaaaggcttcatgatatgccctataggtcaaattcttacattccacagaaatacagaaaacgggtgtttttgctccattgccatatatcggtttgtatggacgacccatagtcggacaaaagctatgctgtcaatcttggcaaatcccctttctctttcttgtgcgatcgaggaggggattactgttttcagtcctcgttacggcatcttgagaggtgaatttattcgatggtagtgctgcttttgaagtatccggctatcagccggtagtagggcttataagacgataatgtactattgatatataaaaaagatggaattgtttactccggttcggtctttttatcgtttctttgtgtcagtctatttgtccgtttttcatctccggacgaaaagggggggctgcctaaatcctacagggcggtagcatgtaaggccgggcctcttccccttacaggcaaatggatggcatactaaacgatgaatatagacaagcatct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agatgcttgtctatattcatcgtttagtatgccatccatttgcctgtaaggggaagaggcccggccttacatgctaccgccctgtaggatttaggcagcccccccttttcgtccggagatgaaaaacggacaaatagactgacacaaagaaacgataaaaagaccgaaccggagtaaacaattccatcttttttatatatcaatagtacattatcgtcttataagccctactaccggctgatagccggatacttcaaaagcagcactaccatcgaataaattcacctctcaagatgccgtaacgaggactgaaaacagtaatcccctcctcgatcgcacaagaaagagaaaggggatttgccaagattgacagcatagcttttgtccgactatgggtcgtccatacaaaccgatatatggcaatggagcaaaaacacccgttttctgtatttctgtggaatgtaagaatttgacctatagggcatatcatgaagcctttgtcctttgtaccataaagcctccatactttatactgtaaaccctctaccctttatactataaagcctccatactttataccgtaaaccctctaccctttatactataaagcccccaccctttatactatgaagtcttttcccttgatactgccctccaaaggatattctccaagagtccctatggaggaatcctcaggcagccttctcgtggttgccctatgccatagagtttaccacatacgaaaagggtgtgccaaaatcgtcttttgattgcgatttcaacacacccttccggcttattgtccggaggaaaaattccggcttatt Mask Tandem Repeat Region ================================================ aataagccggaatttttcctccggacaataagccggaagggtgtgttgaaatcgcaatcaaaagacgattttggcacacccttttcgtatgtggtaaactctatggcatagggcaaccacgagaaggctgcctgaggattcctccatagggactcttggagaatatcctttggagggcagtatcaagggaaaagacttcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnacaaaggcttcatgatatgccctataggtcaaattcttacattccacagaaatacagaaaacgggtgtttttgctccattgccatatatcggtttgtatggacgacccatagtcggacaaaagctatgctgtcaatcttggcaaatcccctttctctttcttgtgcgatcgaggaggggattactgttttcagtcctcgttacggcatcttgagaggtgaatttattcgatggtagtgctgcttttgaagtatccggctatcagccggtagtagggcttataagacgataatgtactattgatatataaaaaagatggaattgtttactccggttcggtctttttatcgtttctttgtgtcagtctatttgtccgtttttcatctccggacgaaaagggggggctgcctaaatcctacagggcggtagcatgtaaggccgggcctcttccccttacaggcaaatggatggcatactaaacgatgaatatagacaagcatct Find is-nt database================================================ Query_seq: PG0537:PG0538|PG0537:PG0538:aminoacyl-histidine dipeptidase:outer membrane efflux protein:->->:588455..589281 827 aataagccggaatttttcctccggacaataagccggaagggtgtgttgaaatcgcaatcaaaagacgattttggcacacccttttcgtatgtggtaaactctatggcatagggcaaccacgagaaggctgcctgaggattcctccatagggactcttggagaatatcctttggagggcagtatcaagggaaaagacttcatagtataaagggtgggggctttatagtataaagggtagagggtttacggtataaagtatggaggctttatagtataaagggtagagggtttacagtataaagtatggaggctttatggtacaaaggacaaaggcttcatgatatgccctataggtcaaattcttacattccacagaaatacagaaaacgggtgtttttgctccattgccatatatcggtttgtatggacgacccatagtcggacaaaagctatgctgtcaatcttggcaaatcccctttctctttcttgtgcgatcgaggaggggattactgttttcagtcctcgttacggcatcttgagaggtgaatttattcgatggtagtgctgcttttgaagtatccggctatcagccggtagtagggcttataagacgataatgtactattgatatataaaaaagatggaattgtttactccggttcggtctttttatcgtttctttgtgtcagtctatttgtccgtttttcatctccggacgaaaagggggggctgcctaaatcctacagggcggtagcatgtaaggccgggcctcttccccttacaggcaaatggatggcatactaaacgatgaatatagacaagcatct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0537:PG0538|PG0537:PG0538:aminoacyl-histidine dipeptidase:outer membrane efflux protein:->->:588455..589281 827 aataagccggaatttttcctccggacaataagccggaagggtgtgttgaaatcgcaatcaaaagacgattttggcacacccttttcgtatgtggtaaactctatggcatagggcaaccacgagaaggctgcctgaggattcctccatagggactcttggagaatatcctttggagggcagtatcaagggaaaagacttcatagtataaagggtgggggctttatagtataaagggtagagggtttacggtataaagtatggaggctttatagtataaagggtagagggtttacagtataaagtatggaggctttatggtacaaaggacaaaggcttcatgatatgccctataggtcaaattcttacattccacagaaatacagaaaacgggtgtttttgctccattgccatatatcggtttgtatggacgacccatagtcggacaaaagctatgctgtcaatcttggcaaatcccctttctctttcttgtgcgatcgaggaggggattactgttttcagtcctcgttacggcatcttgagaggtgaatttattcgatggtagtgctgcttttgaagtatccggctatcagccggtagtagggcttataagacgataatgtactattgatatataaaaaagatggaattgtttactccggttcggtctttttatcgtttctttgtgtcagtctatttgtccgtttttcatctccggacgaaaagggggggctgcctaaatcctacagggcggtagcatgtaaggccgggcctcttccccttacaggcaaatggatggcatactaaacgatgaatatagacaagcatct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0537:PG0538|PG0537:PG0538:aminoacyl-histidine dipeptidase:outer membrane efflux protein:->->:588455..589281 827 aataagccggaatttttcctccggacaataagccggaagggtgtgttgaaatcgcaatcaaaagacgattttggcacacccttttcgtatgtggtaaactctatggcatagggcaaccacgagaaggctgcctgaggattcctccatagggactcttggagaatatcctttggagggcagtatcaagggaaaagacttcatagtataaagggtgggggctttatagtataaagggtagagggtttacggtataaagtatggaggctttatagtataaagggtagagggtttacagtataaagtatggaggctttatggtacaaaggacaaaggcttcatgatatgccctataggtcaaattcttacattccacagaaatacagaaaacgggtgtttttgctccattgccatatatcggtttgtatggacgacccatagtcggacaaaagctatgctgtcaatcttggcaaatcccctttctctttcttgtgcgatcgaggaggggattactgttttcagtcctcgttacggcatcttgagaggtgaatttattcgatggtagtgctgcttttgaagtatccggctatcagccggtagtagggcttataagacgataatgtactattgatatataaaaaagatggaattgtttactccggttcggtctttttatcgtttctttgtgtcagtctatttgtccgtttttcatctccggacgaaaagggggggctgcctaaatcctacagggcggtagcatgtaaggccgggcctcttccccttacaggcaaatggatggcatactaaacgatgaatatagacaagcatct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aataagccggaatttttcctccggacaataagccggaagggtgtgttgaaatcgcaatcaaaagacgattttggcacacccttttcgtatgtggtaaactctatggcatagggcaaccacgagaaggctgcctgaggattcctccatagggactcttggagaatatcctttggagggcagtatcaagggaaaagacttcatagtataaagggtgggggctttatagtataaagggtagagggtttacggtataaagtatggaggctttatagtataaagggtagagggtttacagtataaagtatggaggctttatggtacaaaggacaaaggcttcatgatatgccctataggtcaaattcttacattccacagaaatacagaaaacgggtgtttttgctccattgccatatatcggtttgtatggacgacccatagtcggacaaaagctatgctgtcaatcttggcaaatcccctttctctttcttgtgcgatcgaggaggggattactgttttcagtcctcgttacggcatcttgagaggtgaatttattcgatggtagtgctgcttttgaagtatccggctatcagccggtagtagggcttataagacgataatgtactattgatatataaaaaagatggaattgtttactccggttcggtctttttatcgtttctttgtgtcagtctatttgtccgtttttcatctccggacgaaaagggggggctgcctaaatcctacagggcggtagcatgtaaggccgggcctcttccccttacaggcaaatggatggcatactaaacgatgaatatagacaagcatct Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 12 End: 146 ........... M F P P D N K P E G C V E I A I K R R F W H T L F V C G K L Y G I G Q P R E G C L R I P P ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 44 Strand: + Start: 304 End: 435 ............................................................................................................................................................................................................................................................................................................... M E A L W Y K G Q R L H D M P Y R S N S Y I P Q K Y R K R V F L L H C H I S V C M D D P ........................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 53 Strand: + Start: 378 End: 536 ......................................................................................................................................................................................................................................................................................................................................................................................... M Q K T G V F A P L P Y I G L Y G R P I V G Q K L C C Q S W Q I P F L F L V R S R R G L L F S V L V T A S ................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 338 End: 559 ................................................................................................................................................................................................................................................................................................................................................. M I C P I G Q I L T F H R N T E N G C F C S I A I Y R F V W T T H S R T K A M L S I L A N P L S L S C A I E E G I T V F ............................................................................................................................................................................................................................................................................ Protein_Len: 40 Strand: + Start: 632 End: 751 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M K K M E L F T P V R S F Y R F F V S V Y L S V F H L R T K R G G C L N P T G R ............................................................................ Protein_Len: 59 Strand: + Start: 625 End: 801 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M I Y K K D G I V Y S G S V F L S F L C V S L F V R F S S P D E K G G L P K S Y R A V A C K A G P L P L T G K W M A Y .......................... Protein_Len: 39 Strand: - Start: 709 End: 825 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... R R V F L P P Q R F G V P R Y C T L G P R K G K C A F P H C V L R H I Y V L M .. Protein_Len: 32 Strand: - Start: 552 End: 647 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E I T T S S K F Y G A I L R Y Y P K Y S S L T S N I Y L F S P M .................................................................................................................................................................................... Protein_Len: 54 Strand: - Start: 358 End: 519 ..................................................................................................................................................................................................................................................................................................................................................................... I R V N W L F V S F P H K Q E M A M Y R N T H V V W L R V F A I S D I K A F G R E R E Q A I S S P I V T K M .................................................................................................................................................................................................................................................................................................................... Protein_Len: 48 Strand: - Start: 293 End: 436 .................................................................................................................................................................................................................................................................................................... L I F Y P P K I T C L V F A E H Y A R Y T L N K C E V S I C F V P T K A G N G Y I P K Y P R G M ....................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 47 Strand: - Start: 61 End: 201 ............................................................ F V I K A C G K R I H Y V R H C L A V V L L S G S S E E M P V R P S Y G K S P L I L P F S K M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 54 Strand: - Start: 3 End: 164 .. L G S N K R R V I L R F P T N F D C D F S S K P V G K E Y T T F E I A Y P L W S F A A Q P N R W L S E Q L M ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... aataagccggaatttttcctccggacaataagccggaagggtgtgttgaaatcgcaatcaaaagacgattttggcacacccttttcgtatgtggtaaactctatggcatagggcaaccacgagaaggctgcctgaggattcctccatagggactcttggagaatatcctttggagggcagtatcaagggaaaagacttcatagtataaagggtgggggctttatagtataaagggtagagggtttacggtataaagtatggaggctttatagtataaagggtagagggtttacagtataaagtatggaggctttatggtacaaaggacaaaggcttcatgatatgccctataggtcaaattcttacattccacagaaatacagaaaacgggtgtttttgctccattgccatatatcggtttgtatggacgacccatagtcggacaaaagctatgctgtcaatcttggcaaatcccctttctctttcttgtgcgatcgaggaggggattactgttttcagtcctcgttacggcatcttgagaggtgaatttattcgatggtagtgctgcttttgaagtatccggctatcagccggtagtagggcttataagacgataatgtactattgatatataaaaaagatggaattgtttactccggttcggtctttttatcgtttctttgtgtcagtctatttgtccgtttttcatctccggacgaaaagggggggctgcctaaatcctacagggcggtagcatgtaaggccgggcctcttccccttacaggcaaatggatggcatactaaacgatgaatatagacaagcatct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ PromScan Matrix: RpoN Strand: + Score: 81 Start: 71 End: 87 ......................................................................TTGGCACACCCTTTTCG.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... aataagccggaatttttcctccggacaataagccggaagggtgtgttgaaatcgcaatcaaaagacgattttggcacacccttttcgtatgtggtaaactctatggcatagggcaaccacgagaaggctgcctgaggattcctccatagggactcttggagaatatcctttggagggcagtatcaagggaaaagacttcatagtataaagggtgggggctttatagtataaagggtagagggtttacggtataaagtatggaggctttatagtataaagggtagagggtttacagtataaagtatggaggctttatggtacaaaggacaaaggcttcatgatatgccctataggtcaaattcttacattccacagaaatacagaaaacgggtgtttttgctccattgccatatatcggtttgtatggacgacccatagtcggacaaaagctatgctgtcaatcttggcaaatcccctttctctttcttgtgcgatcgaggaggggattactgttttcagtcctcgttacggcatcttgagaggtgaatttattcgatggtagtgctgcttttgaagtatccggctatcagccggtagtagggcttataagacgataatgtactattgatatataaaaaagatggaattgtttactccggttcggtctttttatcgtttctttgtgtcagtctatttgtccgtttttcatctccggacgaaaagggggggctgcctaaatcctacagggcggtagcatgtaaggccgggcctcttccccttacaggcaaatggatggcatactaaacgatgaatatagacaagcatct Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 84 HP_score: -8.6 Tail_Score: -4.40237 Start: 39 End: 81 Full_Region: GACAATAAGCCGGAA GGGTGTGTTGAAATCG CAATCAAAAGA CGATTTTGGCACACCC TTTTCGTATGTGGTA ......................................GGGTGTGTTGAAATCGCAATCAAAAGACGATTTTGGCACACCC.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 55 HP_score: -3.7 Tail_Score: -3.75051 Start: 196 End: 233 Full_Region: cgtaaaccctctacc ctttatactataaag cccccacc ctttatactatgaag tcttttcccttgata ...................................................................................................................................................................................................ctttatactataaagcccccaccctttatactatgaag.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Find igs database================================================ Query_seq: PG0537:PG0538|PG0537:PG0538:aminoacyl-histidine dipeptidase:outer membrane efflux protein:->->:588455..589281 827 aataagccggaatttttcctccggacaataagccggaagggtgtgttgaaatcgcaatcaaaagacgattttggcacacccttttcgtatgtggtaaactctatggcatagggcaaccacgagaaggctgcctgaggattcctccatagggactcttggagaatatcctttggagggcagtatcaagggaaaagacttcatagtataaagggtgggggctttatagtataaagggtagagggtttacggtataaagtatggaggctttatagtataaagggtagagggtttacagtataaagtatggaggctttatggtacaaaggacaaaggcttcatgatatgccctataggtcaaattcttacattccacagaaatacagaaaacgggtgtttttgctccattgccatatatcggtttgtatggacgacccatagtcggacaaaagctatgctgtcaatcttggcaaatcccctttctctttcttgtgcgatcgaggaggggattactgttttcagtcctcgttacggcatcttgagaggtgaatttattcgatggtagtgctgcttttgaagtatccggctatcagccggtagtagggcttataagacgataatgtactattgatatataaaaaagatggaattgtttactccggttcggtctttttatcgtttctttgtgtcagtctatttgtccgtttttcatctccggacgaaaagggggggctgcctaaatcctacagggcggtagcatgtaaggccgggcctcttccccttacaggcaaatggatggcatactaaacgatgaatatagacaagcatct Intra-Species Hit: Count: 1 Min: 1 Max: 827 Len: 827 Subject: NC_002950_PG0537_PG0538|aminoacyl-histidine dipeptidase:outer membrane efflux protein|POSITIVE:POSITIVE|[588455,589281]|827 HSP 1 e-value: 0.0 bit: 1598.0 Len: 827 Query Start:1 Query End:827 Subject Strand: POSITIVE Subject Start: 1 Subject End: 827 aataagccggaatttttcctccggacaataagccggaagggtgtgttgaaatcgcaatcaaaagacgattttggcacacccttttcgtatgtggtaaactctatggcatagggcaaccacgagaaggctgcctgaggattcctccatagggactcttggagaatatcctttggagggcagtatcaagggaaaagacttcatagtataaagggtgggggctttatagtataaagggtagagggtttacggtataaagtatggaggctttatagtataaagggtagagggtttacagtataaagtatggaggctttatggtacaaaggacaaaggcttcatgatatgccctataggtcaaattcttacattccacagaaatacagaaaacgggtgtttttgctccattgccatatatcggtttgtatggacgacccatagtcggacaaaagctatgctgtcaatcttggcaaatcccctttctctttcttgtgcgatcgaggaggggattactgttttcagtcctcgttacggcatcttgagaggtgaatttattcgatggtagtgctgcttttgaagtatccggctatcagccggtagtagggcttataagacgataatgtactattgatatataaaaaagatggaattgtttactccggttcggtctttttatcgtttctttgtgtcagtctatttgtccgtttttcatctccggacgaaaannnnnnnctgcctaaatcctacagggcggtagcatgtaaggccgggcctcttccccttacaggcaaatggatggcatactaaacgatgaatatagacaagcatct aataagccggaatttttcctccggacaataagccggaagggtgtgttgaaatcgcaatcaaaagacgattttggcacacccttttcgtatgtggtaaactctatggcatagggcaaccacgagaaggctgcctgaggattcctccatagggactcttggagaatatcctttggagggcagtatcaagggaaaagacttcatagtataaagggtgggggctttatagtataaagggtagagggtttacggtataaagtatggaggctttatagtataaagggtagagggtttacagtataaagtatggaggctttatggtacaaaggacaaaggcttcatgatatgccctataggtcaaattcttacattccacagaaatacagaaaacgggtgtttttgctccattgccatatatcggtttgtatggacgacccatagtcggacaaaagctatgctgtcaatcttggcaaatcccctttctctttcttgtgcgatcgaggaggggattactgttttcagtcctcgttacggcatcttgagaggtgaatttattcgatggtagtgctgcttttgaagtatccggctatcagccggtagtagggcttataagacgataatgtactattgatatataaaaaagatggaattgtttactccggttcggtctttttatcgtttctttgtgtcagtctatttgtccgtttttcatctccggacgaaaagggggggctgcctaaatcctacagggcggtagcatgtaaggccgggcctcttccccttacaggcaaatggatggcatactaaacgatgaatatagacaagcatct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAAGCCGGAAUUUUUCCUCCGGACAAUAAGCCGGAAGGGUGUGUUGAAAUCGCAAUCAAAAGACGAUUUUGGCACACCCUUUUCGUAUGUGGUAAACUCUAUGGCAUAGGGCAACCACGAGAAGGCUGCCUGAGGAUUCCUCCAUAGGGACUCUUGGAGAAUAUCCUUUGGAGGGCAGUAUCAAGGGAAAAGACUUCAUAGUAUAAAGGGUGGGGGCUUUAUAGUAUAAAGGGUAGAGGGUUUACGGUAUAAAGUAUGGAGGCUUUAUAGUAUAAAGGGUAGAGGGUUUACAGUAUAAAGUAUGGAGGCUUUAUGGUACAAAGGACAAAGGCUUCAUGAUAUGCCCUAUAGGUCAAAUUCUUACAUUCCACAGAAAUACAGAAAACGGGUGUUUUUGCUCCAUUGCCAUAUAUCGGUUUGUAUGGACGACCCAUAGUCGGACAAAAGCUAUGCUGUCAAUCUUGGCAAAUCCCCUUUCUCUUUCUUGUGCGAUCGAGGAGGGGAUUACUGUUUUCAGUCCUCGUUACGGCAUCUUGAGAGGUGAAUUUAUUCGAUGGUAGUGCUGCUUUUGAAGUAUCCGGCUAUCAGCCGGUAGUAGGGCUUAUAAGACGAUAAUGUACUAUUGAUAUAUAAAAAAGAUGGAAUUGUUUACUCCGGUUCGGUCUUUUUAUCGUUUCUUUGUGUCAGUCUAUUUGUCCGUUUUUCAUCUCCGGACGAAAAGGGGGGGCUGCCUAAAUCCUACAGGGCGGUAGCAUGUAAGGCCGGGCCUCUUCCCCUUACAGGCAAAUGGAUGGCAUACUAAACGAUGAAUAUAGACAAGCAUCU ....(((((((((..((((.((((......(((((((.((((((((..(((((((........).))))))..)))))))).(((((...(..(((....((((.(.(((((...(((.(((((..((((..((((((((((((((.((((((((...)))....))))))))))))..((((....((((....(((.(((.(((((((........))))))).))).))).((.((((((((((..((((((((......))))))))......((((((.......(((........)))..((((((((.((..........))))))))))......))))))....)).)))))))))).))))......)))).((((((((..(((((((.(((....(((((((......)))))))..(((.....)))))))))))))......((((....)))).(((((((((.(((..((......))..)))))))))))).)))))))).)))))))...)))).)))))...)))...))))).).))))...)))..)...)))))..))))))).....))))((((((..(((((......)))).)..))))))...............))))..))....)))))))..((((.((((((((((...(((((((.((((((((((.((.((((.((....)).)))).(((((((..((((....((((((..((....)).))).)))..))))..)))))))..)).)))))))))))))))))..))))))))))...))))........ (-266.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAUGCUUGUCUAUAUUCAUCGUUUAGUAUGCCAUCCAUUUGCCUGUAAGGGGAAGAGGCCCGGCCUUACAUGCUACCGCCCUGUAGGAUUUAGGCAGCCCCCCCUUUUCGUCCGGAGAUGAAAAACGGACAAAUAGACUGACACAAAGAAACGAUAAAAAGACCGAACCGGAGUAAACAAUUCCAUCUUUUUUAUAUAUCAAUAGUACAUUAUCGUCUUAUAAGCCCUACUACCGGCUGAUAGCCGGAUACUUCAAAAGCAGCACUACCAUCGAAUAAAUUCACCUCUCAAGAUGCCGUAACGAGGACUGAAAACAGUAAUCCCCUCCUCGAUCGCACAAGAAAGAGAAAGGGGAUUUGCCAAGAUUGACAGCAUAGCUUUUGUCCGACUAUGGGUCGUCCAUACAAACCGAUAUAUGGCAAUGGAGCAAAAACACCCGUUUUCUGUAUUUCUGUGGAAUGUAAGAAUUUGACCUAUAGGGCAUAUCAUGAAGCCUUUGUCCUUUGUACCAUAAAGCCUCCAUACUUUAUACUGUAAACCCUCUACCCUUUAUACUAUAAAGCCUCCAUACUUUAUACCGUAAACCCUCUACCCUUUAUACUAUAAAGCCCCCACCCUUUAUACUAUGAAGUCUUUUCCCUUGAUACUGCCCUCCAAAGGAUAUUCUCCAAGAGUCCCUAUGGAGGAAUCCUCAGGCAGCCUUCUCGUGGUUGCCCUAUGCCAUAGAGUUUACCACAUACGAAAAGGGUGUGCCAAAAUCGUCUUUUGAUUGCGAUUUCAACACACCCUUCCGGCUUAUUGUCCGGAGGAAAAAUUCCGGCUUAUU (((((..(((((((....(((((((..(.((.((((.(((((.((((.(((((..(.(((...((((...((.((((......)))).))..)))).)))))))))(((((((....))))))).)))).))))).)).)).)).)...)))))))..............(((((.....))))).................)))).)))....))))).(((((((......((((.(((((((((((......................(((...........((((....(((.((((..(((((.......(((((.((((((.(((..((......))..)))..)))))).....((((...(((((....((((((((((...(((((((((..(.((((..(((..((((((.(((((.(........))))))..)))))).....)))..)))).)....))))))))))))))......)))))..)))))(((..((..((((((........(((((((.((((((..........(((((..(((((((........)))))))..)))))..........)))))).)))))))........))))))..))..)))........)))).))))).((((((.(((..((((.....)))).))).))))))..))))).(((((((.......))))))).)))).)))))))...........)))..((((((((...(((((((.........)))))))...)))))))))))))).))))).)))).(((.....)))))))))). (-218.39) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 474 780 731 115 64 NC_002950:PG1273|5end_hypothetical_protein_1351837..1352067_NEGATIVE 446 540 491 52 2 NC_002950:PG1554|5end_hypothetical_protein_1635264..1635494_POSITIVE 417 146 101 51 7 NC_002950:PG1407|5end_nitroimidazole_resistance_protein,_putative_1491648..1491878_NEGATIVE 414 758 713 139 89 NC_002950:PG1021|5end_hypothetical_protein_1083592..1083822_NEGATIVE 414 769 722 194 141 NC_002950:PG0638|5end_tetraacyldisaccharide_4'-kinase_691183..691413_NEGATIVE 403 89 41 182 135 NC_002950:PG1281|5end_hypothetical_protein_1358848..1359078_POSITIVE 394 770 721 177 129 NC_002950:PG1794|5end_DNA_polymerase_type_I_1885978..1886208_POSITIVE 390 770 721 110 46 NC_002950:PG0605|5end_hypothetical_protein_662406..662636_NEGATIVE 384 766 725 169 114 NC_002950:PG0746|5end_sensor_histidine_kinase_793903..794133_NEGATIVE 379 504 462 109 43 NC_002950:PG1772|5end_endonuclease_III_1859079..1859309_POSITIVE 379 150 101 210 142 NC_002950:PG0308|5end_electron_transport_complex,_RnfABCDGE_type,_A_subunit_343175..343405_POSITIVE 378 770 721 195 134 NC_002950:PG1496|5end_hypothetical_protein_1573342..1573572_POSITIVE 376 770 722 199 147 NC_002950:PG0143|5end_hydrolase,_carbon-nitrogen_family_166625..166855_NEGATIVE 375 279 231 226 178 NC_002950:PG1127|5end_transcriptional_regulator,_AsnC_Family_1203223..1203453_POSITIVE 373 770 722 60 3 NC_002950:PG0802|5end_alpha_keto_acid_dehydrogenase_complex,_E3_component,_lipoamide_dehydrogenase_859722..859952_NEGATIVE 373 770 722 199 149 NC_002950:PG0607|5end_hypothetical_protein_662552..662782_POSITIVE 372 517 472 206 163 NC_002950:PG1498|5end_hypothetical_protein_1574656..1574886_POSITIVE 371 150 105 61 8 NC_002950:PG0309|5end_thiamine_biosynthesis_lipoprotein_ApbE_345023..345253_NEGATIVE 371 157 113 97 45 NC_002950:PG0147|5end_hypothetical_protein_169667..169897_NEGATIVE 370 767 729 231 182 NC_002950:PG2142|5end_GTP-binding_protein_Era_2247585..2247815_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 487 80 32 70 16 NC_002950:PG0618|3end_alkyl_hydroperoxide_reductase,_C_subunit_671018..671168_POSITIVE 446 540 491 55 5 NC_002950:PG1553|3end_CobN/magnesium_chelatase_family_protein_1635347..1635497_POSITIVE 406 749 709 55 18 NC_002950:PG1630|3end_hypothetical_protein_1712142..1712292_POSITIVE 403 89 41 55 8 NC_002950:PG1280|3end_hypothetical_protein_1358801..1358951_POSITIVE 401 770 721 132 80 NC_002950:PG2091|3end_dihydroneopterin_aldolase_2191610..2191760_POSITIVE 395 776 733 81 30 NC_002950:PG0364|3end_hypothetical_protein_398410..398560_NEGATIVE 394 770 721 127 79 NC_002950:PG1793|3end_1,4-alpha-glucan_branching_enzyme_1886008..1886158_POSITIVE 393 80 34 51 4 NC_002950:PG0537|3end_aminoacyl-histidine_dipeptidase_588394..588544_POSITIVE 390 770 721 65 1 NC_002950:PG0606|3end_hypothetical_protein_662451..662601_NEGATIVE 383 769 722 91 36 NC_002950:PG1874|3end_hypothetical_protein_1969253..1969403_NEGATIVE 381 768 721 124 76 NC_002950:PG1906|3end_ISPg1,_transposase_2006572..2006722_POSITIVE 381 768 721 124 76 NC_002950:PG1624|3end_ISPg1,_transposase_1706188..1706338_NEGATIVE 381 755 711 48 12 NC_002950:PG1473|3end_conjugative_transposon_protein_TraQ_1549761..1549911_NEGATIVE 381 768 721 124 76 NC_002950:PG1448|3end_ISPg1,_transposase_1533449..1533599_POSITIVE 381 768 721 124 76 NC_002950:PG1197|3end_ISPg1,_transposase_1275475..1275625_NEGATIVE 381 768 721 124 76 NC_002950:PG1177|3end_ISPg1,_transposase_1257794..1257944_NEGATIVE 381 768 721 124 76 NC_002950:PG1031|3end_ISPg1,_transposase_1093081..1093231_NEGATIVE 381 768 721 124 76 NC_002950:PG0825|3end_ISPg1,_transposase_882063..882213_NEGATIVE 381 768 721 124 76 NC_002950:PG0184|3end_ISPg1,_transposase_217567..217717_POSITIVE 380 149 104 64 10 NC_002950:PG0506|3end_arginine-specific_cysteine_proteinase_547625..547775_POSITIVE