Origin IGS: agggaactgtaaattatgaggattgtggtttttattctaattccgtacaaatatatgctttattctcagtccctctccgaaaaagagccaaagagaggaactgccgaga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tctcggcagttcctctctttggctctttttcggagagggactgagaataaagcatatatttgtacggaattagaataaaaaccacaatcctcataatttacagttccct Mask Tandem Repeat Region ================================================ agggaactgtaaattatgaggattgtggtttttattctaattccgtacaaatatatgctttattctcagtccctctccgaaaaagagccaaagagaggaactgccgaga Find is-nt database================================================ Query_seq: PG0531:PG0532|PG0531:PG0532:NAD(+) synthetase:hypothetical protein:<-<-:581230..581338 109 agggaactgtaaattatgaggattgtggtttttattctaattccgtacaaatatatgctttattctcagtccctctccgaaaaagagccaaagagaggaactgccgaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0531:PG0532|PG0531:PG0532:NAD(+) synthetase:hypothetical protein:<-<-:581230..581338 109 agggaactgtaaattatgaggattgtggtttttattctaattccgtacaaatatatgctttattctcagtccctctccgaaaaagagccaaagagaggaactgccgaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0531:PG0532|PG0531:PG0532:NAD(+) synthetase:hypothetical protein:<-<-:581230..581338 109 agggaactgtaaattatgaggattgtggtttttattctaattccgtacaaatatatgctttattctcagtccctctccgaaaaagagccaaagagaggaactgccgaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agggaactgtaaattatgaggattgtggtttttattctaattccgtacaaatatatgctttattctcagtccctctccgaaaaagagccaaagagaggaactgccgaga Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 13 End: 108 ............ M M R I V V F I L I P Y K Y M L Y S Q S L S E K E P K R G T A E . agggaactgtaaattatgaggattgtggtttttattctaattccgtacaaatatatgctttattctcagtccctctccgaaaaagagccaaagagaggaactgccgaga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agggaactgtaaattatgaggattgtggtttttattctaattccgtacaaatatatgctttattctcagtccctctccgaaaaagagccaaagagaggaactgccgaga Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0531:PG0532|PG0531:PG0532:NAD(+) synthetase:hypothetical protein:<-<-:581230..581338 109 agggaactgtaaattatgaggattgtggtttttattctaattccgtacaaatatatgctttattctcagtccctctccgaaaaagagccaaagagaggaactgccgaga Intra-Species Hit: Count: 1 Min: 1 Max: 109 Len: 109 Subject: NC_002950_PG0531_PG0532|NAD(+) synthetase:hypothetical protein|NEGATIVE:NEGATIVE|[581230,581338]|109 HSP 1 e-value: 1.0E-55 bit: 216.0 Len: 109 Query Start:1 Query End:109 Subject Strand: POSITIVE Subject Start: 1 Subject End: 109 agggaactgtaaattatgaggattgtggtttttattctaattccgtacaaatatatgctttattctcagtccctctccgaaaaagagccaaagagaggaactgccgaga agggaactgtaaattatgaggattgtggtttttattctaattccgtacaaatatatgctttattctcagtccctctccgaaaaagagccaaagagaggaactgccgaga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGGGAACUGUAAAUUAUGAGGAUUGUGGUUUUUAUUCUAAUUCCGUACAAAUAUAUGCUUUAUUCUCAGUCCCUCUCCGAAAAAGAGCCAAAGAGAGGAACUGCCGAGA .((...........((((.((((((..((....))..))))))))))...................((((.((((((...............)))))).)))))).... (-19.16) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUCGGCAGUUCCUCUCUUUGGCUCUUUUUCGGAGAGGGACUGAGAAUAAAGCAUAUAUUUGUACGGAAUUAGAAUAAAAACCACAAUCCUCAUAAUUUACAGUUCCCU ..((..((((((((((((.............)))))))))))).)).....(((......)))..((((((..((......................))..)))))).. (-22.77) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 887 100 51 191 142 NC_002950:PG0531|5end_NAD(+)_synthetase_581139..581369_NEGATIVE 331 100 53 67 24 NC_002950:PG1249|5end_1-acyl-sn-glycerol-3-phosphate_acetyltransferase,_putative_1327161..1327391_NEGATIVE 264 98 55 145 98 NC_002950:PG1328|5end_CoA_ligase_family_protein_1408680..1408910_NEGATIVE 262 97 56 216 167 NC_002950:PG1702|5end_DNA_gyrase,_B_subunit_1789910..1790140_NEGATIVE 258 99 57 192 153 NC_002950:PG2048|5end_hypothetical_protein_2148514..2148744_NEGATIVE 256 67 22 74 33 NC_002950:PG0366|5end_hypothetical_protein_400815..401045_NEGATIVE 251 100 51 188 140 NC_002950:PG0330|5end_DNA-binding_protein,_histone-like_family_364993..365223_NEGATIVE 247 98 54 68 34 NC_002950:PG2176|5end_ISPg2,_transposase_2284345..2284575_NEGATIVE 247 98 73 212 186 NC_002950:PG2064|5end_hypothetical_protein_2160550..2160780_POSITIVE 247 98 54 68 34 NC_002950:PG1746|5end_ISPg2,_transposase_1832230..1832460_NEGATIVE 247 98 54 68 34 NC_002950:PG1350|5end_ISPg2,_transposase_1430866..1431096_POSITIVE 247 98 54 68 34 NC_002950:PG0865|5end_ISPg2,_transposase_928896..929126_NEGATIVE 247 98 54 68 34 NC_002950:PG0277|5end_ISPg2,_transposase_308856..309086_POSITIVE 246 86 41 153 111 NC_002950:PG1033|5end_hypothetical_protein_1095398..1095628_POSITIVE 242 87 68 227 207 NC_002950:PG1501|5end_transcriptional_regulator,_tetR_family_1579099..1579329_NEGATIVE 242 89 41 227 179 NC_002950:PG1334|5end_band_7/Mec-2_family_protein_1416402..1416632_NEGATIVE 241 80 36 214 170 NC_002950:PG1874|5end_hypothetical_protein_1969660..1969890_NEGATIVE 239 89 44 95 48 NC_002950:PG1893|5end_hypothetical_protein_1990960..1991190_POSITIVE 238 98 56 189 153 NC_002950:PG1093|5end_hypothetical_protein_1158121..1158351_POSITIVE 237 89 44 217 173 NC_002950:PG1163|5end_cobyrinic_acid_a,c-diamide_synthase_1243381..1243611_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 331 100 53 44 1 NC_002950:PG1250|3end_hypothetical_protein_1327184..1327334_NEGATIVE 290 100 66 129 91 NC_002950:PG0795|3end_hypothetical_protein_849603..849753_POSITIVE 278 97 58 42 4 NC_002950:PG2072|3end_UvrD/REP_helicase_domain_protein_2170757..2170907_POSITIVE 261 89 43 66 21 NC_002950:PG1836|3end_nucleoside_permease_NupG_1929858..1930008_POSITIVE 255 100 67 72 38 NC_002950:PG1574|3end_hypothetical_protein_1652461..1652611_NEGATIVE 247 79 42 47 6 NC_002950:PG0052|3end_sensor_histidine_kinase_64686..64836_POSITIVE 246 87 56 141 109 NC_002950:PG1703|3end_MazG_family_protein_1789968..1790118_NEGATIVE 240 100 66 102 69 NC_002950:PG2077|3end_hypothetical_protein_2177005..2177155_NEGATIVE 238 98 56 80 44 NC_002950:PG1091|3end_DHH_subfamily_1_protein_1158092..1158242_POSITIVE 237 98 58 108 67 NC_002950:PG0134|3end_magnesium_transporter_155783..155933_NEGATIVE 236 100 68 72 34 NC_002950:PG1118|3end_clpB_protein_1192083..1192233_NEGATIVE 235 80 48 66 29 NC_002950:PG1259|3end_anaerobic_ribonucleoside-triphosphate_reductase_activating_protein_1334974..1335124_NEGATIVE 230 99 57 59 14 NC_002950:PG1977|3end_hypothetical_protein_2064687..2064837_NEGATIVE 228 97 64 61 34 NC_002950:PG0450|3end_hypothetical_protein_491602..491752_POSITIVE 226 87 42 127 78 NC_002950:PG0564|3end_hypothetical_protein_621416..621566_NEGATIVE 225 47 1 59 4 NC_002950:PG1654|3end_D-alanyl-D-alanine_dipeptidase_1737161..1737311_POSITIVE 224 98 62 82 47 NC_002950:PG1722|3end_hypothetical_protein_1812633..1812783_NEGATIVE 223 43 2 67 26 NC_002950:PG0778|3end_hypothetical_protein_831150..831300_NEGATIVE 221 77 56 30 11 NC_002950:PG2062|3end_histidyl-tRNA_synthetase_2160415..2160565_POSITIVE 220 99 69 63 19 NC_002950:PG1327|3end_aminotransferase,_putative_1405270..1405420_NEGATIVE