Origin IGS: gttctgatgtagtattaggagtgaaactctgaaatttcctgccaaatttatatgctttttcccaaacagccaatctttgatcgcaggtgcagattcgaacaacgtgccggcagcattacgatcgagtttgccctcatcatgtgacaccttgttgtcggaatgaaaaccatgctttttgctatgaatcggttggtgtcatttccttcttatccagctaaggaaataccttatcctgcgagaagaaaaaacgactc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gagtcgttttttcttctcgcaggataaggtatttccttagctggataagaaggaaatgacaccaaccgattcatagcaaaaagcatggttttcattccgacaacaaggtgtcacatgatgagggcaaactcgatcgtaatgctgccggcacgttgttcgaatctgcacctgcgatcaaagattggctgtttgggaaaaagcatataaatttggcaggaaatttcagagtttcactcctaatactacatcagaac Mask Tandem Repeat Region ================================================ gttctgatgtagtattaggagtgaaactctgaaatttcctgccaaatttatatgctttttcccaaacagccaatctttgatcgcaggtgcagattcgaacaacgtgccggcagcattacgatcgagtttgccctcatcatgtgacaccttgttgtcggaatgaaaaccatgctttttgctatgaatcggttggtgtcatttccttcttatccagctaaggaaataccttatcctgcgagaagaaaaaacgactc Find is-nt database================================================ Query_seq: PG0523:PG0524|PG0523:PG0524:inositol-5-monophosphate dehydrogenase:hypothetical protein:<-->:568485..568738 254 gttctgatgtagtattaggagtgaaactctgaaatttcctgccaaatttatatgctttttcccaaacagccaatctttgatcgcaggtgcagattcgaacaacgtgccggcagcattacgatcgagtttgccctcatcatgtgacaccttgttgtcggaatgaaaaccatgctttttgctatgaatcggttggtgtcatttccttcttatccagctaaggaaataccttatcctgcgagaagaaaaaacgactc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0523:PG0524|PG0523:PG0524:inositol-5-monophosphate dehydrogenase:hypothetical protein:<-->:568485..568738 254 gttctgatgtagtattaggagtgaaactctgaaatttcctgccaaatttatatgctttttcccaaacagccaatctttgatcgcaggtgcagattcgaacaacgtgccggcagcattacgatcgagtttgccctcatcatgtgacaccttgttgtcggaatgaaaaccatgctttttgctatgaatcggttggtgtcatttccttcttatccagctaaggaaataccttatcctgcgagaagaaaaaacgactc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0523:PG0524|PG0523:PG0524:inositol-5-monophosphate dehydrogenase:hypothetical protein:<-->:568485..568738 254 gttctgatgtagtattaggagtgaaactctgaaatttcctgccaaatttatatgctttttcccaaacagccaatctttgatcgcaggtgcagattcgaacaacgtgccggcagcattacgatcgagtttgccctcatcatgtgacaccttgttgtcggaatgaaaaccatgctttttgctatgaatcggttggtgtcatttccttcttatccagctaaggaaataccttatcctgcgagaagaaaaaacgactc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gttctgatgtagtattaggagtgaaactctgaaatttcctgccaaatttatatgctttttcccaaacagccaatctttgatcgcaggtgcagattcgaacaacgtgccggcagcattacgatcgagtttgccctcatcatgtgacaccttgttgtcggaatgaaaaccatgctttttgctatgaatcggttggtgtcatttccttcttatccagctaaggaaataccttatcctgcgagaagaaaaaacgactc Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 34 End: 141 ................................. M S C Q I Y M L F P K Q P I F D R R C R F E Q R A G S I T I E F A L I M ................................................................................................................. Protein_Len: 49 Strand: + Start: 14 End: 160 ............. M R S E T L K F P A K F I C F F P N S Q S L I A G A D S N N V P A A L R S S L P S S C D T L L S E .............................................................................................. Protein_Len: 43 Strand: + Start: 87 End: 215 ...................................................................................... M Q I R T T C R Q H Y D R V C P H H V T P C C R N E N H A F C Y E S V G V I S F L S S ....................................... Protein_Len: 31 Strand: + Start: 160 End: 252 ............................................................................................................................................................... M K T M L F A M N R L V S F P S Y P A K E I P Y P A R R K N D .. Protein_Len: 44 Strand: - Start: 51 End: 182 .................................................. I S K G L C G I K S R L H L N S C R A P L M V I S N A R M M H C R T T P I F V M S K A M ........................................................................ Protein_Len: 31 Strand: - Start: 2 End: 94 . E S T T N P T F S Q F K R G F K Y A K E W V A L R Q D C T C M ................................................................................................................................................................ Protein_Len: 60 Strand: - Start: 1 End: 186 H L I L L S V R F N G A L N I H K K G F L W D K I A P A S E F L T G A A N R D L K G E D H S V K N D S H F G H K K S H M .................................................................... gttctgatgtagtattaggagtgaaactctgaaatttcctgccaaatttatatgctttttcccaaacagccaatctttgatcgcaggtgcagattcgaacaacgtgccggcagcattacgatcgagtttgccctcatcatgtgacaccttgttgtcggaatgaaaaccatgctttttgctatgaatcggttggtgtcatttccttcttatccagctaaggaaataccttatcctgcgagaagaaaaaacgactc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gttctgatgtagtattaggagtgaaactctgaaatttcctgccaaatttatatgctttttcccaaacagccaatctttgatcgcaggtgcagattcgaacaacgtgccggcagcattacgatcgagtttgccctcatcatgtgacaccttgttgtcggaatgaaaaccatgctttttgctatgaatcggttggtgtcatttccttcttatccagctaaggaaataccttatcctgcgagaagaaaaaacgactc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0523:PG0524|PG0523:PG0524:inositol-5-monophosphate dehydrogenase:hypothetical protein:<-->:568485..568738 254 gttctgatgtagtattaggagtgaaactctgaaatttcctgccaaatttatatgctttttcccaaacagccaatctttgatcgcaggtgcagattcgaacaacgtgccggcagcattacgatcgagtttgccctcatcatgtgacaccttgttgtcggaatgaaaaccatgctttttgctatgaatcggttggtgtcatttccttcttatccagctaaggaaataccttatcctgcgagaagaaaaaacgactc Intra-Species Hit: Count: 1 Min: 1 Max: 254 Len: 254 Subject: NC_002950_PG0523_PG0524|inositol-5-monophosphate dehydrogenase:hypothetical protein|NEGATIVE:POSITIVE|[568485,568738]|254 HSP 1 e-value: 1.0E-142 bit: 504.0 Len: 254 Query Start:1 Query End:254 Subject Strand: POSITIVE Subject Start: 1 Subject End: 254 gttctgatgtagtattaggagtgaaactctgaaatttcctgccaaatttatatgctttttcccaaacagccaatctttgatcgcaggtgcagattcgaacaacgtgccggcagcattacgatcgagtttgccctcatcatgtgacaccttgttgtcggaatgaaaaccatgctttttgctatgaatcggttggtgtcatttccttcttatccagctaaggaaataccttatcctgcgagaagaaaaaacgactc gttctgatgtagtattaggagtgaaactctgaaatttcctgccaaatttatatgctttttcccaaacagccaatctttgatcgcaggtgcagattcgaacaacgtgccggcagcattacgatcgagtttgccctcatcatgtgacaccttgttgtcggaatgaaaaccatgctttttgctatgaatcggttggtgtcatttccttcttatccagctaaggaaataccttatcctgcgagaagaaaaaacgactc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUCUGAUGUAGUAUUAGGAGUGAAACUCUGAAAUUUCCUGCCAAAUUUAUAUGCUUUUUCCCAAACAGCCAAUCUUUGAUCGCAGGUGCAGAUUCGAACAACGUGCCGGCAGCAUUACGAUCGAGUUUGCCCUCAUCAUGUGACACCUUGUUGUCGGAAUGAAAACCAUGCUUUUUGCUAUGAAUCGGUUGGUGUCAUUUCCUUCUUAUCCAGCUAAGGAAAUACCUUAUCCUGCGAGAAGAAAAAACGACUC (((..((...(((((..((((.....)))).((((((......))))))..)))))...))...))).(((((((.......(.(((.((((((((((.(...((((.....))))...).)))))))))))))).((((.(((((......)))))..))))....(((((.....)).)))....)))))))(((.(((..(((((.(((((.(((((.....)))))..))).)).))))).))).))).. (-59.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAGUCGUUUUUUCUUCUCGCAGGAUAAGGUAUUUCCUUAGCUGGAUAAGAAGGAAAUGACACCAACCGAUUCAUAGCAAAAAGCAUGGUUUUCAUUCCGACAACAAGGUGUCACAUGAUGAGGGCAAACUCGAUCGUAAUGCUGCCGGCACGUUGUUCGAAUCUGCACCUGCGAUCAAAGAUUGGCUGUUUGGGAAAAAGCAUAUAAAUUUGGCAGGAAAUUUCAGAGUUUCACUCCUAAUACUACAUCAGAAC .(((((.(((((....(((((((....((((((((((...((.....)).)))))))...)))....(((((..(((((...((.((((...((((..((((......))))..((((((((......))).)))))))))..))))))...))))).)))))....)))))))..))))).)))))..((((((....((.((......))))..((((((....))))))..)))))).............. (-61.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1035 130 81 221 172 NC_002950:PG0523|5end_inositol-5-monophosphate_dehydrogenase_568394..568624_NEGATIVE 406 157 111 181 114 NC_002950:PG0582|5end_cell_division_protein_FtsQ,_putative_639600..639830_POSITIVE 398 125 81 66 24 NC_002950:PG1247|5end_3-dehydroquinate_synthase_1324444..1324674_POSITIVE 397 148 102 74 7 NC_002950:PG0729|5end_D-alanylalanine_synthetase_780818..781048_NEGATIVE 392 138 94 210 153 NC_002950:PG1847|5end_endoribonuclease_L-PSP,_putative_1946607..1946837_NEGATIVE 372 149 105 101 37 NC_002950:PG0659|5end_hypothetical_protein_709148..709378_NEGATIVE 370 148 103 89 44 NC_002950:PG2112|5end_hypothetical_protein_2221471..2221701_POSITIVE 369 115 73 66 21 NC_002950:PG1595|5end_ribulose-phosphate_3-epimerase_1674250..1674480_NEGATIVE 365 146 103 74 29 NC_002950:PG1992|5end_glucose-inhibited_division_protein_A_2081410..2081640_POSITIVE 364 135 92 221 174 NC_002950:PG0006|5end_MATE_efflux_family_protein_6332..6562_NEGATIVE 362 126 85 222 181 NC_002950:PG1579|5end_ATPase,_MoxR_family_1656493..1656723_POSITIVE 362 128 81 218 162 NC_002950:PG0271|5end_single-stranded_binding_protein_303842..304072_POSITIVE 355 120 74 58 9 NC_002950:PG0378|5end_elongation_factor_Ts_407904..408134_POSITIVE 354 115 77 204 157 NC_002950:PG2185|5end_transporter,_putative_2293249..2293479_NEGATIVE 351 119 75 40 1 NC_002950:PG0082|5end_hypothetical_protein_96044..96274_POSITIVE 348 112 74 42 1 NC_002950:PG0768|5end_hypothetical_protein_822381..822611_NEGATIVE 343 134 94 188 149 NC_002950:PG1594|5end_ComEC/Rec2-related_protein_1673587..1673817_NEGATIVE 340 207 167 206 160 NC_002950:PG0451|5end_CBS_domain_protein_491519..491749_POSITIVE 337 133 93 197 157 NC_002950:PG1372|5end_hypothetical_protein_1454722..1454952_NEGATIVE 337 130 81 215 157 NC_002950:PG0359|5end_flavin_reductase_domain_protein_392851..393081_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 422 127 83 76 30 NC_002950:PG0226|3end_transglutaminase-related_protein_259838..259988_NEGATIVE 398 125 81 51 9 NC_002950:PG1246|3end_alanyl-tRNA_synthetase_1324509..1324659_POSITIVE 372 149 105 108 44 NC_002950:PG0660|3end_nitroreductase_family_protein_709141..709291_NEGATIVE 365 150 102 116 79 NC_002950:PG0555|3end_DNA-binding_protein,_histone-like_family_611716..611866_NEGATIVE 362 126 85 121 80 NC_002950:PG1578|3end_quinolinate_synthetase_1656472..1656622_POSITIVE 334 120 71 146 98 NC_002950:PG1816|3end_NAD(P)H_dehydrogenase,_quinone_family,_putative_1911430..1911580_NEGATIVE 334 126 85 133 93 NC_002950:PG1608|3end_methylmalonyl-CoA_decarboxylase,_beta_subunit_1688685..1688835_NEGATIVE 331 120 80 96 60 NC_002950:PG0333|3end_hypothetical_protein_368811..368961_POSITIVE 326 120 72 59 10 NC_002950:PG1064|3end_dihydroorotate_dehydrogenase,_putative_1132425..1132575_POSITIVE 320 125 82 149 95 NC_002950:PG1638|3end_thioredoxin_family_protein_1718664..1718814_NEGATIVE 319 159 115 142 101 NC_002950:PG1173|3end_YkgG_family_protein_1253129..1253279_POSITIVE 319 127 82 146 81 NC_002950:PG0085|3end_alpha-galactosidase_100556..100706_POSITIVE 317 129 83 80 38 NC_002950:PG0132|3end_hypothetical_protein_153054..153204_POSITIVE 315 114 75 151 106 NC_002950:PG0270|3end_redox-sensitive_transcriptional_activator_OxyR_303871..304021_POSITIVE 310 89 51 37 6 NC_002950:PG1067|3end_hypothetical_protein_1134688..1134838_POSITIVE 309 130 83 148 97 NC_002950:PG0259|3end_hypothetical_protein_293701..293851_POSITIVE 308 132 103 102 66 NC_002950:PG1972|3end_hemagglutinin_protein_HagB_2061203..2061353_POSITIVE 308 220 171 150 103 NC_002950:PG0935|3end_4-diphosphocytidyl-2C-methyl-D-erythritol_kinase_994297..994447_NEGATIVE 308 141 102 66 27 NC_002950:PG0803|3end_glucosamine-6-phosphate_deaminase_859727..859877_NEGATIVE 307 133 91 109 65 NC_002950:PG0057|3end_nicotinate_phosphoribosyltransferase_70999..71149_POSITIVE