Origin IGS: aggctttgcgctaatccccgacagatgacgtttgaaaaaaaggccggctcgtcttccctgaaagacgaaacaaagtcggtgaatatcttcgccgtactctttgtagtagtgggcag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctgcccactactacaaagagtacggcgaagatattcaccgactttgtttcgtctttcagggaagacgagccggcctttttttcaaacgtcatctgtcggggattagcgcaaagcct Mask Tandem Repeat Region ================================================ aggctttgcgctaatccccgacagatgacgtttgaaaaaaaggccggctcgtcttccctgaaagacgaaacaaagtcggtgaatatcttcgccgtactctttgtagtagtgggcag Find is-nt database================================================ Query_seq: PG0517:PG0518|PG0517:PG0518:hypothetical protein:abortive infection protein family:<-<-:562188..562303 116 aggctttgcgctaatccccgacagatgacgtttgaaaaaaaggccggctcgtcttccctgaaagacgaaacaaagtcggtgaatatcttcgccgtactctttgtagtagtgggcag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0517:PG0518|PG0517:PG0518:hypothetical protein:abortive infection protein family:<-<-:562188..562303 116 aggctttgcgctaatccccgacagatgacgtttgaaaaaaaggccggctcgtcttccctgaaagacgaaacaaagtcggtgaatatcttcgccgtactctttgtagtagtgggcag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0517:PG0518|PG0517:PG0518:hypothetical protein:abortive infection protein family:<-<-:562188..562303 116 aggctttgcgctaatccccgacagatgacgtttgaaaaaaaggccggctcgtcttccctgaaagacgaaacaaagtcggtgaatatcttcgccgtactctttgtagtagtgggcag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aggctttgcgctaatccccgacagatgacgtttgaaaaaaaggccggctcgtcttccctgaaagacgaaacaaagtcggtgaatatcttcgccgtactctttgtagtagtgggcag Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 25 End: 114 ........................ M T F E K K A G S S S L K D E T K S V N I F A V L F V V V G .. Protein_Len: 38 Strand: - Start: 3 End: 116 .. S Q A L G R C I V N S F F A P E D E R F S S V F D T F I K A T S K T T T P M aggctttgcgctaatccccgacagatgacgtttgaaaaaaaggccggctcgtcttccctgaaagacgaaacaaagtcggtgaatatcttcgccgtactctttgtagtagtgggcag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aggctttgcgctaatccccgacagatgacgtttgaaaaaaaggccggctcgtcttccctgaaagacgaaacaaagtcggtgaatatcttcgccgtactctttgtagtagtgggcag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0517:PG0518|PG0517:PG0518:hypothetical protein:abortive infection protein family:<-<-:562188..562303 116 aggctttgcgctaatccccgacagatgacgtttgaaaaaaaggccggctcgtcttccctgaaagacgaaacaaagtcggtgaatatcttcgccgtactctttgtagtagtgggcag Intra-Species Hit: Count: 1 Min: 1 Max: 116 Len: 116 Subject: NC_002950_PG0517_PG0518|hypothetical protein:abortive infection protein family|NEGATIVE:NEGATIVE|[562188,562303]|116 HSP 1 e-value: 3.0E-47 bit: 188.0 Len: 116 Query Start:1 Query End:116 Subject Strand: POSITIVE Subject Start: 1 Subject End: 116 aggctttgcgctaatccccgacagatgacgtttgnnnnnnnggccggctcgtcttccctgaaagacgaaacaaagtcggtgaatatcttcgccgtactctttgtagtagtgggcag aggctttgcgctaatccccgacagatgacgtttgaaaaaaaggccggctcgtcttccctgaaagacgaaacaaagtcggtgaatatcttcgccgtactctttgtagtagtgggcag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGGCUUUGCGCUAAUCCCCGACAGAUGACGUUUGAAAAAAAGGCCGGCUCGUCUUCCCUGAAAGACGAAACAAAGUCGGUGAAUAUCUUCGCCGUACUCUUUGUAGUAGUGGGCAG .(((.....))).........(((((...)))))........(((.(((((((((......))))))..((((((.(((((((....)))))))....))))))...))).))).. (-32.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUGCCCACUACUACAAAGAGUACGGCGAAGAUAUUCACCGACUUUGUUUCGUCUUUCAGGGAAGACGAGCCGGCCUUUUUUUCAAACGUCAUCUGUCGGGGAUUAGCGCAAAGCCU ..((....((((......))))..(((.....((((.(((((.....(((((((((....)))))))))..(((.(((.....))).)))....)))))))))...)))...)).. (-28.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1007 50 1 141 92 NC_002950:PG0517|5end_hypothetical_protein_562097..562327_NEGATIVE 392 86 42 91 35 NC_002950:PG0422|5end_hypothetical_protein_462302..462532_NEGATIVE 351 50 2 67 4 NC_002950:PG0153|5end_aspartyl-tRNA_synthetase_177430..177660_NEGATIVE 348 50 2 153 100 NC_002950:PG1719|5end_ABC_transporter,_ATP-binding_protein,_MsbA_family_1806749..1806979_POSITIVE 347 87 41 197 151 NC_002950:PG2029|5end_hypothetical_protein_2126543..2126773_POSITIVE 342 48 2 112 65 NC_002950:PG1721|5end_ribonuclease_R_1810284..1810514_POSITIVE 335 49 1 79 18 NC_002950:PG2128|5end_ISPg5,_transposase_Orf2_2234805..2235035_NEGATIVE 335 49 1 79 18 NC_002950:PG2057|5end_ISPg5,_transposase_Orf2_2155947..2156177_NEGATIVE 335 49 1 79 18 NC_002950:PG1420|5end_ISPg5,_transposase_Orf2_1505446..1505676_POSITIVE 335 49 1 79 18 NC_002950:PG0943|5end_ISPg5,_transposase_Orf2_1002512..1002742_POSITIVE 335 49 1 79 18 NC_002950:PG0458|5end_ISPg5,_transposase_Orf2_501463..501693_NEGATIVE 335 49 1 79 18 NC_002950:PG0426|5end_ISPg5,_transposase_Orf2_465098..465328_NEGATIVE 335 49 1 79 18 NC_002950:PG0041|5end_ISPg5_transposase_Orf2_48377..48607_POSITIVE 335 49 1 79 18 NC_002950:PG0008|5end_ISPg5_transposase_Orf2_8023..8253_NEGATIVE 331 68 31 180 140 NC_002950:PG0259|5end_hypothetical_protein_292278..292508_POSITIVE 326 59 17 138 93 NC_002950:PG0011|5end_glycosyl_hydrolase,_family_3_11494..11724_POSITIVE 319 110 75 75 35 NC_002950:PG1776|5end_dnaJ_protein_1865471..1865701_POSITIVE 318 60 15 74 17 NC_002950:PG1950|5end_hypothetical_protein_2039600..2039830_POSITIVE 317 60 18 43 4 NC_002950:PG0728|5end_hypothetical_protein_779807..780037_NEGATIVE 312 49 6 79 35 NC_002950:PG1710|5end_ISPg5,_transposase_Orf2_1795357..1795587_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 352 57 17 140 106 NC_002950:PG0282|3end_ABC_transporter,_ATP-binding_protein_316244..316394_NEGATIVE 323 85 42 89 50 NC_002950:PG1133|3end_hypothetical_protein_1214103..1214253_POSITIVE 322 48 1 132 87 NC_002950:PG0685|3end_ABC_transporter,_ATP-binding_protein_740855..741005_POSITIVE 308 48 1 50 5 NC_002950:PG1852|3end_exonuclease_1951194..1951344_NEGATIVE 305 57 16 89 48 NC_002950:PG1162|3end_ATP:cob(I)alamin_adenosyltransferase,_putative_1241473..1241623_NEGATIVE 304 65 21 86 41 NC_002950:PG0016|3end_sigma-54_dependent_DNA-binding_response_regulator_18376..18526_POSITIVE 302 47 1 83 32 NC_002950:PG2032|3end_primosomal_protein_N'_2130868..2131018_NEGATIVE 302 50 1 99 32 NC_002950:PG0605|3end_hypothetical_protein_661165..661315_NEGATIVE 301 64 22 119 73 NC_002950:PG1715|3end_hypothetical_protein_1803393..1803543_NEGATIVE 298 82 41 144 95 NC_002950:PG1497|3end_DNA-binding_protein,_histone-like_family_1574635..1574785_POSITIVE 297 34 1 99 61 NC_002950:PG1841|3end_hypothetical_protein_1938313..1938463_POSITIVE 296 48 4 101 55 NC_002950:PG2121|3end_L-asparaginase_2228545..2228695_NEGATIVE 296 59 15 103 48 NC_002950:PG0722|3end_hypothetical_protein_774739..774889_NEGATIVE 296 80 41 42 2 NC_002950:PG0004|3end_NAD-dependent_deacetylase_3752..3902_POSITIVE 295 94 57 81 43 NC_002950:PG2092|3end_hypothetical_protein_2191651..2191801_NEGATIVE 293 48 1 150 107 NC_002950:PG0723|3end_hypothetical_protein_775355..775505_POSITIVE 288 58 16 63 17 NC_002950:PG2126|3end_conserved_hypothetical_protein_TIGR00044_2232438..2232588_NEGATIVE 285 80 40 143 108 NC_002950:PG0363|3end_hypothetical_protein_398377..398527_POSITIVE 284 47 3 67 11 NC_002950:PG1371|3end_phosphorylase_family_protein_1452352..1452502_NEGATIVE 284 89 44 147 102 NC_002950:PG0033|3end_RmuC_domain_protein_40242..40392_NEGATIVE