Origin IGS: actggcaactaattttgtgtacttgtataaagagtaaataagacagtcattacggagaaaaaagatttggttatttttgtcttcacagaaaggctgctctgcataatggcttctctatattcaaggagccggtaatgaattgaaaaagagaagattgaatagaattgcaaaacatagaaatgctaccactcatgacccaccgcctcacatcaatcggaatatcctcttcgatcggatatgctgcagcgatagacctcgatcccggtcgggattgtagagaacgaatccatcagatatgcaaatcatagatcgaccgatagcatcatagcataggacaagctcctgcgctcctgtatcgcacccgccgaagttctacggagaactcggcgggtgcttcttttatatcggcttcggaaaaggccttttcgagcctgcgatttatatacgaatcacttacgatctatatatttattgaaaacgatttatatataaaacgaaaaccatttatatataaattgaaagtcatttatatacaaatcgttattcgactcttctgaaaacgaatttcgaagttgggaagacagttttctatccttttttaccttggatgcctctcccttttggatgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggtgcaagttttttagtggaaattagaaagagggaggagagtggtaagggcttgccggaacgctatggaagtgacaaaaagtatccggactcaacaccacatgccaaataaagaaaaaactgatcaggaaccggttacaggctaaaaatcgcccgaattcgccttcctaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttaggaaggcgaattcgggcgatttttagcctgtaaccggttcctgatcagttttttctttatttggcatgtggtgttgagtccggatactttttgtcacttccatagcgttccggcaagcccttaccactctcctccctctttctaatttccactaaaaaacttgcaccaaaaaagttttcaacaacgaagacgagtttggaatgattccaaataagcatccaaaagggagaggcatccaaggtaaaaaaggatagaaaactgtcttcccaacttcgaaattcgttttcagaagagtcgaataacgatttgtatataaatgactttcaatttatatataaatggttttcgttttatatataaatcgttttcaataaatatatagatcgtaagtgattcgtatataaatcgcaggctcgaaaaggccttttccgaagccgatataaaagaagcacccgccgagttctccgtagaacttcggcgggtgcgatacaggagcgcaggagcttgtcctatgctatgatgctatcggtcgatctatgatttgcatatctgatggattcgttctctacaatcccgaccgggatcgaggtctatcgctgcagcatatccgatcgaagaggatattccgattgatgtgaggcggtgggtcatgagtggtagcatttctatgttttgcaattctattcaatcttctctttttcaattcattaccggctccttgaatatagagaagccattatgcagagcagcctttctgtgaagacaaaaataaccaaatcttttttctccgtaatgactgtcttatttactctttatacaagtacacaaaattagttgccagt Mask Tandem Repeat Region ================================================ actggcaactaattttgtgtacttgtataaagagtaaataagacagtcattacggagaaaaaagatttggttatttttgtcttcacagaaaggctgctctgcataatggcttctctatattcaaggagccggtaatgaattgaaaaagagaagattgaatagaattgcaaaacatagaaatgctaccactcatgacccaccgcctcacatcaatcggaatatcctcttcgatcggatatgctgcagcgatagacctcgatcccggtcgggattgtagagaacgaatccatcagatatgcaaatcatagatcgaccgatagcatcatagcataggacaagctcctgcgctcctgtatcgcacccgccgaagttctacggagaactcggcgggtgcttcttttatatcggcttcggaaaaggccttttcgagcctgcgatttatatacgaatcacttacgatctatatatttattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncaaatcgttattcgactcttctgaaaacgaatttcgaagttgggaagacagttttctatccttttttaccttggatgcctctcccttttggatgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggtgcaagttttttagtggaaattagaaagagggaggagagtggtaagggcttgccggaacgctatggaagtgacaaaaagtatccggactcaacaccacatgccaaataaagaaaaaactgatcaggaaccggttacaggctaaaaatcgcccgaattcgccttcctaa Find is-nt database================================================ Query_seq: PG0404:PG0408|PG0404:PG0408:hypothetical protein:hypothetical protein:<-<-:440197..441041 845 actggcaactaattttgtgtacttgtataaagagtaaataagacagtcattacggagaaaaaagatttggttatttttgtcttcacagaaaggctgctctgcataatggcttctctatattcaaggagccggtaatgaattgaaaaagagaagattgaatagaattgcaaaacatagaaatgctaccactcatgacccaccgcctcacatcaatcggaatatcctcttcgatcggatatgctgcagcgatagacctcgatcccggtcgggattgtagagaacgaatccatcagatatgcaaatcatagatcgaccgatagcatcatagcataggacaagctcctgcgctcctgtatcgcacccgccgaagttctacggagaactcggcgggtgcttcttttatatcggcttcggaaaaggccttttcgagcctgcgatttatatacgaatcacttacgatctatatatttattgaaaacgatttatatataaaacgaaaaccatttatatataaattgaaagtcatttatatacaaatcgttattcgactcttctgaaaacgaatttcgaagttgggaagacagttttctatccttttttaccttggatgcctctcccttttggatgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggtgcaagttttttagtggaaattagaaagagggaggagagtggtaagggcttgccggaacgctatggaagtgacaaaaagtatccggactcaacaccacatgccaaataaagaaaaaactgatcaggaaccggttacaggctaaaaatcgcccgaattcgccttcctaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0404:PG0408|PG0404:PG0408:hypothetical protein:hypothetical protein:<-<-:440197..441041 845 actggcaactaattttgtgtacttgtataaagagtaaataagacagtcattacggagaaaaaagatttggttatttttgtcttcacagaaaggctgctctgcataatggcttctctatattcaaggagccggtaatgaattgaaaaagagaagattgaatagaattgcaaaacatagaaatgctaccactcatgacccaccgcctcacatcaatcggaatatcctcttcgatcggatatgctgcagcgatagacctcgatcccggtcgggattgtagagaacgaatccatcagatatgcaaatcatagatcgaccgatagcatcatagcataggacaagctcctgcgctcctgtatcgcacccgccgaagttctacggagaactcggcgggtgcttcttttatatcggcttcggaaaaggccttttcgagcctgcgatttatatacgaatcacttacgatctatatatttattgaaaacgatttatatataaaacgaaaaccatttatatataaattgaaagtcatttatatacaaatcgttattcgactcttctgaaaacgaatttcgaagttgggaagacagttttctatccttttttaccttggatgcctctcccttttggatgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggtgcaagttttttagtggaaattagaaagagggaggagagtggtaagggcttgccggaacgctatggaagtgacaaaaagtatccggactcaacaccacatgccaaataaagaaaaaactgatcaggaaccggttacaggctaaaaatcgcccgaattcgccttcctaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0404:PG0408|PG0404:PG0408:hypothetical protein:hypothetical protein:<-<-:440197..441041 845 actggcaactaattttgtgtacttgtataaagagtaaataagacagtcattacggagaaaaaagatttggttatttttgtcttcacagaaaggctgctctgcataatggcttctctatattcaaggagccggtaatgaattgaaaaagagaagattgaatagaattgcaaaacatagaaatgctaccactcatgacccaccgcctcacatcaatcggaatatcctcttcgatcggatatgctgcagcgatagacctcgatcccggtcgggattgtagagaacgaatccatcagatatgcaaatcatagatcgaccgatagcatcatagcataggacaagctcctgcgctcctgtatcgcacccgccgaagttctacggagaactcggcgggtgcttcttttatatcggcttcggaaaaggccttttcgagcctgcgatttatatacgaatcacttacgatctatatatttattgaaaacgatttatatataaaacgaaaaccatttatatataaattgaaagtcatttatatacaaatcgttattcgactcttctgaaaacgaatttcgaagttgggaagacagttttctatccttttttaccttggatgcctctcccttttggatgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggtgcaagttttttagtggaaattagaaagagggaggagagtggtaagggcttgccggaacgctatggaagtgacaaaaagtatccggactcaacaccacatgccaaataaagaaaaaactgatcaggaaccggttacaggctaaaaatcgcccgaattcgccttcctaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 actggcaactaattttgtgtacttgtataaagagtaaataagacagtcattacggagaaaaaagatttggttatttttgtcttcacagaaaggctgctctgcataatggcttctctatattcaaggagccggtaatgaattgaaaaagagaagattgaatagaattgcaaaacatagaaatgctaccactcatgacccaccgcctcacatcaatcggaatatcctcttcgatcggatatgctgcagcgatagacctcgatcccggtcgggattgtagagaacgaatccatcagatatgcaaatcatagatcgaccgatagcatcatagcataggacaagctcctgcgctcctgtatcgcacccgccgaagttctacggagaactcggcgggtgcttcttttatatcggcttcggaaaaggccttttcgagcctgcgatttatatacgaatcacttacgatctatatatttattgaaaacgatttatatataaaacgaaaaccatttatatataaattgaaagtcatttatatacaaatcgttattcgactcttctgaaaacgaatttcgaagttgggaagacagttttctatccttttttaccttggatgcctctcccttttggatgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggtgcaagttttttagtggaaattagaaagagggaggagagtggtaagggcttgccggaacgctatggaagtgacaaaaagtatccggactcaacaccacatgccaaataaagaaaaaactgatcaggaaccggttacaggctaaaaatcgcccgaattcgccttcctaa Predict ORF larger than 30AA ================================================ Protein_Len: 52 Strand: + Start: 119 End: 274 ...................................................................................................................... M Q G A G N E L K K R R L N R I A K H R N A T T H D P P P H I N R N I L F D R I C C S D R P R S R S G L ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 49 Strand: + Start: 159 End: 305 .............................................................................................................................................................. M E L Q N I E M L P L M T H R L T S I G I S S S I G Y A A A I D L D P G R D C R E R I H Q I C K S ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 49 Strand: + Start: 343 End: 489 ...................................................................................................................................................................................................................................................................................................................................................... M R S C I A P A E V L R R T R R V L L L Y R L R K R P F R A C D L Y T N H L R S I Y L L K T I Y I .................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 330 End: 554 ......................................................................................................................................................................................................................................................................................................................................... M G Q A P A L L Y R T R R S S T E N S A G A S F I S A S E K A F S S L R F I Y E S L T I Y I F I E N D L Y I K R K P F I ................................................................................................................................................................................................................................................................................................... Protein_Len: 33 Strand: + Start: 564 End: 662 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M S K L G R Q F S I L F Y L G C L S L L D A Y L E S F Q T R L R C ....................................................................................................................................................................................... Protein_Len: 58 Strand: + Start: 515 End: 688 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M E S H L Y T N R Y S T L L K T N F E V G K T V F Y P F L P W M P L P F G C L F G I I P N S S S L L K T F L V Q V F ............................................................................................................................................................. Protein_Len: 60 Strand: + Start: 529 End: 783 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M Y K S L F D S S E N E F R S W E D S F L S F F T L D A S P F W M L I W N H S K L V F V V E N F F G A S F L V E I R K R .............................................................. Protein_Len: 34 Strand: + Start: 716 End: 817 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M V R A C R N A M E V T K S I R T Q H H M P N K E K T D Q E P V T G ............................ Protein_Len: 34 Strand: - Start: 701 End: 802 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ F S P P S H Y P S A P V S H F H C F T D P S L V V H W I F F F S I M ........................................... Protein_Len: 49 Strand: - Start: 486 End: 632 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... I Y F S F W K Y I F Q F D N I Y L D N N S E E S F S N R L Q S S L K R D K K V K S A E G K Q I S M ..................................................................................................................................................................................................................... Protein_Len: 45 Strand: - Start: 326 End: 460 ..................................................................................................................................................................................................................................................................................................................................... L M P C A G A S R Y R V R R L E V S F E A P A E K I D A E S F A K E L R R N I Y S D S V M ................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 37 Strand: - Start: 208 End: 318 ............................................................................................................................................................................................................... M L R F I R K S R I H Q L S L G R D R D P N Y L V F G D S I C I M S R G M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 86 End: 286 ..................................................................................... P K E I N L S G T I F Q F L S S Q I S N C F M S I S G S M V W R R V D I P I D E E I P Y A A A I S R S G P R S Q L S R M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 53 Strand: - Start: 12 End: 170 ........... N Q T S T Y L T F L V T M V S F F S K T I K T K V S L S S Q M I A E R Y E L L R Y H I S F S F I S Y F Q M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... actggcaactaattttgtgtacttgtataaagagtaaataagacagtcattacggagaaaaaagatttggttatttttgtcttcacagaaaggctgctctgcataatggcttctctatattcaaggagccggtaatgaattgaaaaagagaagattgaatagaattgcaaaacatagaaatgctaccactcatgacccaccgcctcacatcaatcggaatatcctcttcgatcggatatgctgcagcgatagacctcgatcccggtcgggattgtagagaacgaatccatcagatatgcaaatcatagatcgaccgatagcatcatagcataggacaagctcctgcgctcctgtatcgcacccgccgaagttctacggagaactcggcgggtgcttcttttatatcggcttcggaaaaggccttttcgagcctgcgatttatatacgaatcacttacgatctatatatttattgaaaacgatttatatataaaacgaaaaccatttatatataaattgaaagtcatttatatacaaatcgttattcgactcttctgaaaacgaatttcgaagttgggaagacagttttctatccttttttaccttggatgcctctcccttttggatgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggtgcaagttttttagtggaaattagaaagagggaggagagtggtaagggcttgccggaacgctatggaagtgacaaaaagtatccggactcaacaccacatgccaaataaagaaaaaactgatcaggaaccggttacaggctaaaaatcgcccgaattcgccttcctaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ actggcaactaattttgtgtacttgtataaagagtaaataagacagtcattacggagaaaaaagatttggttatttttgtcttcacagaaaggctgctctgcataatggcttctctatattcaaggagccggtaatgaattgaaaaagagaagattgaatagaattgcaaaacatagaaatgctaccactcatgacccaccgcctcacatcaatcggaatatcctcttcgatcggatatgctgcagcgatagacctcgatcccggtcgggattgtagagaacgaatccatcagatatgcaaatcatagatcgaccgatagcatcatagcataggacaagctcctgcgctcctgtatcgcacccgccgaagttctacggagaactcggcgggtgcttcttttatatcggcttcggaaaaggccttttcgagcctgcgatttatatacgaatcacttacgatctatatatttattgaaaacgatttatatataaaacgaaaaccatttatatataaattgaaagtcatttatatacaaatcgttattcgactcttctgaaaacgaatttcgaagttgggaagacagttttctatccttttttaccttggatgcctctcccttttggatgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggtgcaagttttttagtggaaattagaaagagggaggagagtggtaagggcttgccggaacgctatggaagtgacaaaaagtatccggactcaacaccacatgccaaataaagaaaaaactgatcaggaaccggttacaggctaaaaatcgcccgaattcgccttcctaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 43 HP_score: -3.2 Tail_Score: -2.69151 Start: 328 End: 348 Full_Region: ACCGATAGCATCATA GCATAGGA CAAGC TCCTGCGC TCCTGTATCGCACCC .......................................................................................................................................................................................................................................................................................................................................GCATAGGACAAGCTCCTGCGC................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 44 HP_score: -3.2 Tail_Score: -2.84812 Start: 328 End: 348 Full_Region: gggtgcgatacagga gcgcagga gcttg tcctatgc tatgatgctatcggt .......................................................................................................................................................................................................................................................................................................................................gcgcaggagcttgtcctatgc................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 93 HP_score: -11.3 Tail_Score: -4.55118 Start: 355 End: 397 Full_Region: CTCCTGCGCTCCTGT ATCGCACCCGCCGAAGTTCT ACGG AGAACTCGGCGGGTGCTTC TTTTATATCGGCTTC ..................................................................................................................................................................................................................................................................................................................................................................ATCGCACCCGCCGAAGTTCTACGGAGAACTCGGCGGGTGCTTC................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 100 HP_score: -21.8 Tail_Score: -4.38141 Start: 358 End: 394 Full_Region: CTGCGCTCCTGTATC GCACCCGCCGAAGTTCT ACGG AGAACTCGGCGGGTGC TTCTTTTATATCGGC .....................................................................................................................................................................................................................................................................................................................................................................GCACCCGCCGAAGTTCTACGGAGAACTCGGCGGGTGC................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 81 HP_score: -7.6 Tail_Score: -4.61868 Start: 495 End: 547 Full_Region: cgttttcagaagagt cgaataacgatttgtatataaatga ctt tcaatttatatataaatggttttcg ttttatatataaatc ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cgaataacgatttgtatataaatgactttcaatttatatataaatggttttcg.......................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 75 HP_score: -5.4 Tail_Score: -4.75518 Start: 501 End: 540 Full_Region: agaagagtcgaataa cgatttgtatataaatga ctt tcaatttatatataaatgg ttttcgttttatata ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cgatttgtatataaatgactttcaatttatatataaatgg................................................................................................................................................................................................................................................................................................................. Find igs database================================================ Query_seq: PG0404:PG0408|PG0404:PG0408:hypothetical protein:hypothetical protein:<-<-:440197..441041 845 actggcaactaattttgtgtacttgtataaagagtaaataagacagtcattacggagaaaaaagatttggttatttttgtcttcacagaaaggctgctctgcataatggcttctctatattcaaggagccggtaatgaattgaaaaagagaagattgaatagaattgcaaaacatagaaatgctaccactcatgacccaccgcctcacatcaatcggaatatcctcttcgatcggatatgctgcagcgatagacctcgatcccggtcgggattgtagagaacgaatccatcagatatgcaaatcatagatcgaccgatagcatcatagcataggacaagctcctgcgctcctgtatcgcacccgccgaagttctacggagaactcggcgggtgcttcttttatatcggcttcggaaaaggccttttcgagcctgcgatttatatacgaatcacttacgatctatatatttattgaaaacgatttatatataaaacgaaaaccatttatatataaattgaaagtcatttatatacaaatcgttattcgactcttctgaaaacgaatttcgaagttgggaagacagttttctatccttttttaccttggatgcctctcccttttggatgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggtgcaagttttttagtggaaattagaaagagggaggagagtggtaagggcttgccggaacgctatggaagtgacaaaaagtatccggactcaacaccacatgccaaataaagaaaaaactgatcaggaaccggttacaggctaaaaatcgcccgaattcgccttcctaa Intra-Species Hit: Count: 3 Min: 1 Max: 845 Len: 845 Subject: NC_002950_PG0404_PG0408|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[440197,441041]|845 HSP 1 e-value: 0.0 bit: 914.0 Len: 461 Query Start:1 Query End:461 Subject Strand: POSITIVE Subject Start: 1 Subject End: 461 actggcaactaattttgtgtacttgtataaagagtaaataagacagtcattacggagaaaaaagatttggttatttttgtcttcacagaaaggctgctctgcataatggcttctctatattcaaggagccggtaatgaattgaaaaagagaagattgaatagaattgcaaaacatagaaatgctaccactcatgacccaccgcctcacatcaatcggaatatcctcttcgatcggatatgctgcagcgatagacctcgatcccggtcgggattgtagagaacgaatccatcagatatgcaaatcatagatcgaccgatagcatcatagcataggacaagctcctgcgctcctgtatcgcacccgccgaagttctacggagaactcggcgggtgcttcttttatatcggcttcggaaaaggccttttcgagcctgcgatttatatacgaatcacttacgatc................................................................................................................................................................................................................................................................................................................................................................................................ actggcaactaattttgtgtacttgtataaagagtaaataagacagtcattacggagaaaaaagatttggttatttttgtcttcacagaaaggctgctctgcataatggcttctctatattcaaggagccggtaatgaattgaaaaagagaagattgaatagaattgcaaaacatagaaatgctaccactcatgacccaccgcctcacatcaatcggaatatcctcttcgatcggatatgctgcagcgatagacctcgatcccggtcgggattgtagagaacgaatccatcagatatgcaaatcatagatcgaccgatagcatcatagcataggacaagctcctgcgctcctgtatcgcacccgccgaagttctacggagaactcggcgggtgcttcttttatatcggcttcggaaaaggccttttcgagcctgcgatttatatacgaatcacttacgatc................................................................................................................................................................................................................................................................................................................................................................................................ HSP 2 e-value: 0.0 bit: 642.0 Len: 324 Query Start:522 Query End:845 Subject Strand: POSITIVE Subject Start: 522 Subject End: 845 .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gtcatttatatacaaatcgttattcgactcttctgaaaacgaatttcgaagttgggaagacagttttctatccttttttaccttggatgcctctcccttttggatgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggtgcaagttttttagtggaaattagaaagagggaggagagtggtaagggcttgccggaacgctatggaagtgacaaaaagtatccggactcaacaccacatgccaaataaagaaaaaactgatcaggaaccggttacaggctaaaaatcgcccgaattcgccttcctaa .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gtcatttatatacaaatcgttattcgactcttctgaaaacgaatttcgaagttgggaagacagttttctatccttttttaccttggatgcctctcccttttggatgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttggtgcaagttttttagtggaaattagaaagagggaggagagtggtaagggcttgccggaacgctatggaagtgacaaaaagtatccggactcaacaccacatgccaaataaagaaaaaactgatcaggaaccggttacaggctaaaaatcgcccgaattcgccttcctaa Subject: NC_002950_PG0609_PG0610|hypothetical protein:hypothetical protein|POSITIVE:NEGATIVE|[663416,663693]|278 HSP 1 e-value: 4.0E-23 bit: 111.0 Len: 104 Query Start:358 Query End:461 Subject Strand: NEGATIVE Subject Start: 94 Subject End: 197 .....................................................................................................................................................................................................................................................................................................................................................................gcacccgccgaagttctacggagaactcggcgggtgcttcttttatatcggcttcggaaaaggccttttcgagcctgcgatttatatacgaatcacttacgatc................................................................................................................................................................................................................................................................................................................................................................................................ .....................................................................................................................................................................................................................................................................................................................................................................gcacccgtcggagttctccgtagaactcggcgggtgcttcttttatatcagctactgaaaaggctttttagagcctgcgatctatatacaaatcatttacgatc................................................................................................................................................................................................................................................................................................................................................................................................ Subject: NC_002950_PG1145_PG1148|long-chain-fatty-acid--CoA ligase, putative:hypothetical protein|NEGATIVE:POSITIVE|[1230396,1231343]|948 HSP 1 e-value: 1.0E-20 bit: 103.0 Len: 52 Query Start:626 Query End:677 Subject Strand: POSITIVE Subject Start: 836 Subject End: 887 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttgg........................................................................................................................................................................ .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgcttatttggaatcattccaaactcgtcttcgttgttgaaaacttttttgg........................................................................................................................................................................ HSP 2 e-value: 3.0E-8 bit: 61.9 Len: 70 Query Start:386 Query End:455 Subject Strand: POSITIVE Subject Start: 618 Subject End: 687 .................................................................................................................................................................................................................................................................................................................................................................................................ggcgggtgcttcttttatatcggct-tcggaaaaggccttttcgagcctgcgatttatatacgaatcactt...................................................................................................................................................................................................................................................................................................................................................................................................... .................................................................................................................................................................................................................................................................................................................................................................................................ggcgggtgcctcttttatttcggctctcgaaaaagcctttttcg-gcctgcgatttatatacaaatcactt...................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACUGGCAACUAAUUUUGUGUACUUGUAUAAAGAGUAAAUAAGACAGUCAUUACGGAGAAAAAAGAUUUGGUUAUUUUUGUCUUCACAGAAAGGCUGCUCUGCAUAAUGGCUUCUCUAUAUUCAAGGAGCCGGUAAUGAAUUGAAAAAGAGAAGAUUGAAUAGAAUUGCAAAACAUAGAAAUGCUACCACUCAUGACCCACCGCCUCACAUCAAUCGGAAUAUCCUCUUCGAUCGGAUAUGCUGCAGCGAUAGACCUCGAUCCCGGUCGGGAUUGUAGAGAACGAAUCCAUCAGAUAUGCAAAUCAUAGAUCGACCGAUAGCAUCAUAGCAUAGGACAAGCUCCUGCGCUCCUGUAUCGCACCCGCCGAAGUUCUACGGAGAACUCGGCGGGUGCUUCUUUUAUAUCGGCUUCGGAAAAGGCCUUUUCGAGCCUGCGAUUUAUAUACGAAUCACUUACGAUCUAUAUAUUUAUUGAAAACGAUUUAUAUAUAAAACGAAAACCAUUUAUAUAUAAAUUGAAAGUCAUUUAUAUACAAAUCGUUAUUCGACUCUUCUGAAAACGAAUUUCGAAGUUGGGAAGACAGUUUUCUAUCCUUUUUUACCUUGGAUGCCUCUCCCUUUUGGAUGCUUAUUUGGAAUCAUUCCAAACUCGUCUUCGUUGUUGAAAACUUUUUUGGUGCAAGUUUUUUAGUGGAAAUUAGAAAGAGGGAGGAGAGUGGUAAGGGCUUGCCGGAACGCUAUGGAAGUGACAAAAAGUAUCCGGACUCAACACCACAUGCCAAAUAAAGAAAAAACUGAUCAGGAACCGGUUACAGGCUAAAAAUCGCCCGAAUUCGCCUUCCUAA ..(((((.....((((((.(((((.((((..(((((....(((.(((((((((((((...((((((......))))))(((((......)))))..)))))..)))))))))))...)))))..(...(((((((.....(((((((((((((((((....(((((((.....((((..((((((.(............................((.....))...((((((.(((.(((......(((.((((((((((((....))))))).)).)......)).)))......))).)))...))))))..).))))))...(((.(((((.....))))).))).))))...(((((((((((.(((((....))))))))))))))))............((((.((((((.....)))))))))).))))))).....(((((.....(((((.(.(((((....(((...(((((((((((((((..........)))))))))))))))....)))...))))).).))))).)))))..((((((.((..((.....))...)).)))))))))))))))...))))))))(((((..((((((((((((((...(((...((((((.....))))))...((.((((....)))).)).........))).....((((((....)))))))))))))).))).))).)))))..)))))))..)..)))).))))))))))).......((........))...)))))...................(((((.(((...(.(((........))).)...)))..))))).. (-224.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAGGAAGGCGAAUUCGGGCGAUUUUUAGCCUGUAACCGGUUCCUGAUCAGUUUUUUCUUUAUUUGGCAUGUGGUGUUGAGUCCGGAUACUUUUUGUCACUUCCAUAGCGUUCCGGCAAGCCCUUACCACUCUCCUCCCUCUUUCUAAUUUCCACUAAAAAACUUGCACCAAAAAAGUUUUCAACAACGAAGACGAGUUUGGAAUGAUUCCAAAUAAGCAUCCAAAAGGGAGAGGCAUCCAAGGUAAAAAAGGAUAGAAAACUGUCUUCCCAACUUCGAAAUUCGUUUUCAGAAGAGUCGAAUAACGAUUUGUAUAUAAAUGACUUUCAAUUUAUAUAUAAAUGGUUUUCGUUUUAUAUAUAAAUCGUUUUCAAUAAAUAUAUAGAUCGUAAGUGAUUCGUAUAUAAAUCGCAGGCUCGAAAAGGCCUUUUCCGAAGCCGAUAUAAAAGAAGCACCCGCCGAGUUCUCCGUAGAACUUCGGCGGGUGCGAUACAGGAGCGCAGGAGCUUGUCCUAUGCUAUGAUGCUAUCGGUCGAUCUAUGAUUUGCAUAUCUGAUGGAUUCGUUCUCUACAAUCCCGACCGGGAUCGAGGUCUAUCGCUGCAGCAUAUCCGAUCGAAGAGGAUAUUCCGAUUGAUGUGAGGCGGUGGGUCAUGAGUGGUAGCAUUUCUAUGUUUUGCAAUUCUAUUCAAUCUUCUCUUUUUCAAUUCAUUACCGGCUCCUUGAAUAUAGAGAAGCCAUUAUGCAGAGCAGCCUUUCUGUGAAGACAAAAAUAACCAAAUCUUUUUUCUCCGUAAUGACUGUCUUAUUUACUCUUUAUACAAGUACACAAAAUUAGUUGCCAGU (((((((..((....(((((........)))))....)).)))))))................((((((((((.(((.((((..((.((.((((((((.....((((((((...((((((((((.....(.(((((...(((((....................(((.........((((((......))))))..(((((((.....)))))))..))).......(((((((((..............(((((((....)))))))......(((((.(((((..(((....))).)))))..(((((((((((..(((.((((.(.(((((((((((((((((((..((.....))..))))))))).......)))))))))).).)))).)))..))))))))))).....)))))...)))))))))............))))).((((((((((((((((....))))).))))))))))).....))))).).))).)))))))..)))))))).(((((((.(((.(((...(((......))).))).))).....(((..(.(((((....))))).).(..((((((((....((((((.(((((..(((....)))))))))))))).))))))))..)..))).)))))))......(((((((((....((......(((((((..(((((...............)))))...)))))))....))))))))))).............)))))))))).))..........((........))...........)))).....)))....))))........)))))). (-240.17) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 954 130 81 221 172 NC_002950:PG0404|5end_hypothetical_protein_440106..440336_NEGATIVE 777 420 371 57 8 NC_002950:PG1574|5end_hypothetical_protein_1652613..1652843_NEGATIVE 777 420 371 63 14 NC_002950:PG0371|5end_hypothetical_protein_404043..404273_POSITIVE 742 680 631 124 75 NC_002950:PG0609|5end_hypothetical_protein_663183..663413_POSITIVE 685 680 631 57 8 NC_002950:PG0995|5end_hypothetical_protein_1055585..1055815_NEGATIVE 626 677 631 66 20 NC_002950:PG0773|5end_hypothetical_protein_825131..825361_POSITIVE 607 680 631 89 38 NC_002950:PG1357|5end_hypothetical_protein_1436458..1436688_NEGATIVE 555 468 423 151 107 NC_002950:PG1223|5end_hypothetical_protein_1299461..1299691_POSITIVE 447 270 227 204 160 NC_002950:PG0934|5end_hypothetical_protein_994318..994548_NEGATIVE 440 278 231 78 24 NC_002950:PG1388|5end_hypothetical_protein_1470889..1471119_NEGATIVE 436 408 362 219 177 NC_002950:PG0227|5end_DNA_repair_protein_RadA_263978..264208_NEGATIVE 435 276 231 61 2 NC_002950:PG1783|5end_glycosyl_transferase,_group_2_family_protein_1871868..1872098_POSITIVE 415 368 321 225 177 NC_002950:PG0987|5end_hypothetical_protein_1048002..1048232_POSITIVE 398 379 338 230 190 NC_002950:PG1475|5end_conjugative_transposon_protein_TraN_1551835..1552065_NEGATIVE 392 279 232 185 133 NC_002950:PG0141|5end_spoOJ_protein_164747..164977_NEGATIVE 389 739 698 163 109 NC_002950:PG2107|5end_thiamine_biosynthesis_protein_ThiH_2216658..2216888_NEGATIVE 389 390 343 70 18 NC_002950:PG1852|5end_exonuclease_1951973..1952203_NEGATIVE 387 269 226 193 123 NC_002950:PG0744|5end_RNA_methyltransferase,_TrmH_family_791897..792127_NEGATIVE 387 389 343 48 1 NC_002950:PG0362|5end_hypothetical_protein_395301..395531_POSITIVE 386 694 651 50 1 NC_002950:PG1167|5end_hypothetical_protein_1248427..1248657_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 681 406 361 150 105 NC_002950:PG0371|3end_hypothetical_protein_404224..404374_POSITIVE 672 678 631 56 9 NC_002950:PG1868|3end_hypothetical_protein_1965098..1965248_NEGATIVE 628 280 232 68 14 NC_002950:PG1574|3end_hypothetical_protein_1652461..1652611_NEGATIVE 626 677 631 59 13 NC_002950:PG0772|3end_hypothetical_protein_825204..825354_POSITIVE 605 658 611 151 104 NC_002950:PG0995|3end_hypothetical_protein_1055469..1055619_NEGATIVE 585 400 353 59 9 NC_002950:PG1274|3end_elongation_factor_P_1352489..1352639_POSITIVE 567 400 353 59 9 NC_002950:PG0568|3end_elongation_factor_P_623999..624149_NEGATIVE 558 664 628 151 115 NC_002950:PG1167|3end_hypothetical_protein_1248644..1248794_POSITIVE 443 520 471 56 7 NC_002950:PG0609|3end_hypothetical_protein_663355..663505_POSITIVE 440 278 231 97 43 NC_002950:PG1389|3end_DNA-binding_protein,_histone-like_family_1470870..1471020_NEGATIVE 438 367 322 130 76 NC_002950:PG0555|3end_DNA-binding_protein,_histone-like_family_611716..611866_NEGATIVE 435 276 231 74 15 NC_002950:PG1782|3end_hypothetical_protein_1871961..1872111_POSITIVE 411 520 475 46 1 NC_002950:PG0773|3end_hypothetical_protein_825348..825498_POSITIVE 403 480 433 62 10 NC_002950:PG1357|3end_hypothetical_protein_1436327..1436477_NEGATIVE 401 370 321 124 75 NC_002950:PG0361|3end_hypothetical_protein_395481..395631_POSITIVE 401 390 346 79 26 NC_002950:PG0332|3end_transcription_termination_factor_Rho_367675..367825_POSITIVE 400 270 223 106 66 NC_002950:PG1012|3end_tRNA-i(6)A37_modification_enzyme_MiaB_1070089..1070239_NEGATIVE 394 739 701 45 2 NC_002950:PG0672|3end_iron_compound_ABC_transporter,_ATP-binding_protein_723840..723990_POSITIVE 393 390 343 101 39 NC_002950:PG0320|3end_hypothetical_protein_354577..354727_POSITIVE 389 739 698 150 96 NC_002950:PG2108|3end_thiazole_synthase_2216671..2216821_NEGATIVE