Origin IGS: aaggtcttcttttttcattgtttgttagttaatcgaagaatcgacgaccgcgcgacctttcgaccgaggatcgtctatgtttctattctgtcgttacggcaacgaaggtagtgaaaagttgttggcagtgtggctgttcgttcgttttcgatctgattttacgcgccaccgatcgtccgc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gcggacgatcggtggcgcgtaaaatcagatcgaaaacgaacgaacagccacactgccaacaacttttcactaccttcgttgccgtaacgacagaatagaaacatagacgatcctcggtcgaaaggtcgcgcggtcgtcgattcttcgattaactaacaaacaatgaaaaaagaagacctt Mask Tandem Repeat Region ================================================ aaggtcttcttttttcattgtttgttagttaatcgaagaatcgacgaccgcgcgacctttcgaccgaggatcgtctatgtttctattctgtcgttacggcaacgaaggtagtgaaaagttgttggcagtgtggctgttcgttcgttttcgatctgattttacgcgccaccgatcgtccgc Find is-nt database================================================ Query_seq: PG0343:PG0344|PG0343:PG0344:methionine gamma-lyase:purple acid phosphatase:<-<-:378453..378632 180 aaggtcttcttttttcattgtttgttagttaatcgaagaatcgacgaccgcgcgacctttcgaccgaggatcgtctatgtttctattctgtcgttacggcaacgaaggtagtgaaaagttgttggcagtgtggctgttcgttcgttttcgatctgattttacgcgccaccgatcgtccgc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0343:PG0344|PG0343:PG0344:methionine gamma-lyase:purple acid phosphatase:<-<-:378453..378632 180 aaggtcttcttttttcattgtttgttagttaatcgaagaatcgacgaccgcgcgacctttcgaccgaggatcgtctatgtttctattctgtcgttacggcaacgaaggtagtgaaaagttgttggcagtgtggctgttcgttcgttttcgatctgattttacgcgccaccgatcgtccgc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0343:PG0344|PG0343:PG0344:methionine gamma-lyase:purple acid phosphatase:<-<-:378453..378632 180 aaggtcttcttttttcattgtttgttagttaatcgaagaatcgacgaccgcgcgacctttcgaccgaggatcgtctatgtttctattctgtcgttacggcaacgaaggtagtgaaaagttgttggcagtgtggctgttcgttcgttttcgatctgattttacgcgccaccgatcgtccgc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaggtcttcttttttcattgtttgttagttaatcgaagaatcgacgaccgcgcgacctttcgaccgaggatcgtctatgtttctattctgtcgttacggcaacgaaggtagtgaaaagttgttggcagtgtggctgttcgttcgttttcgatctgattttacgcgccaccgatcgtccgc Predict ORF larger than 30AA ================================================ Protein_Len: 49 Strand: + Start: 32 End: 178 ............................... M E E S T T A R P F D R G S S M F L F C R Y G N E G S E K L L A V W L F V R F R S D F T R H R S S .. aaggtcttcttttttcattgtttgttagttaatcgaagaatcgacgaccgcgcgacctttcgaccgaggatcgtctatgtttctattctgtcgttacggcaacgaaggtagtgaaaagttgttggcagtgtggctgttcgttcgttttcgatctgattttacgcgccaccgatcgtccgc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaggtcttcttttttcattgtttgttagttaatcgaagaatcgacgaccgcgcgacctttcgaccgaggatcgtctatgtttctattctgtcgttacggcaacgaaggtagtgaaaagttgttggcagtgtggctgttcgttcgttttcgatctgattttacgcgccaccgatcgtccgc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0343:PG0344|PG0343:PG0344:methionine gamma-lyase:purple acid phosphatase:<-<-:378453..378632 180 aaggtcttcttttttcattgtttgttagttaatcgaagaatcgacgaccgcgcgacctttcgaccgaggatcgtctatgtttctattctgtcgttacggcaacgaaggtagtgaaaagttgttggcagtgtggctgttcgttcgttttcgatctgattttacgcgccaccgatcgtccgc Intra-Species Hit: Count: 1 Min: 1 Max: 180 Len: 180 Subject: NC_002950_PG0343_PG0344|methionine gamma-lyase:purple acid phosphatase|NEGATIVE:NEGATIVE|[378453,378632]|180 HSP 1 e-value: 8.0E-98 bit: 357.0 Len: 180 Query Start:1 Query End:180 Subject Strand: POSITIVE Subject Start: 1 Subject End: 180 aaggtcttcttttttcattgtttgttagttaatcgaagaatcgacgaccgcgcgacctttcgaccgaggatcgtctatgtttctattctgtcgttacggcaacgaaggtagtgaaaagttgttggcagtgtggctgttcgttcgttttcgatctgattttacgcgccaccgatcgtccgc aaggtcttcttttttcattgtttgttagttaatcgaagaatcgacgaccgcgcgacctttcgaccgaggatcgtctatgtttctattctgtcgttacggcaacgaaggtagtgaaaagttgttggcagtgtggctgttcgttcgttttcgatctgattttacgcgccaccgatcgtccgc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGGUCUUCUUUUUUCAUUGUUUGUUAGUUAAUCGAAGAAUCGACGACCGCGCGACCUUUCGACCGAGGAUCGUCUAUGUUUCUAUUCUGUCGUUACGGCAACGAAGGUAGUGAAAAGUUGUUGGCAGUGUGGCUGUUCGUUCGUUUUCGAUCUGAUUUUACGCGCCACCGAUCGUCCGC ..((((....((((((((((((((((.(((...((((((((.((.(((...((((((((......)))).))))....))))).))))).)))....)))))))))..))))))))))....((((.((((((..(((.(.(((....))).).))).))))))))))..))))...... (-44.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCGGACGAUCGGUGGCGCGUAAAAUCAGAUCGAAAACGAACGAACAGCCACACUGCCAACAACUUUUCACUACCUUCGUUGCCGUAACGACAGAAUAGAAACAUAGACGAUCCUCGGUCGAAAGGUCGCGCGGUCGUCGAUUCUUCGAUUAACUAACAAACAAUGAAAAAAGAAGACCUU ..((.((....(((((.(((.........(((....))))))....)))))..))))......(((((......(((((((...((((((.(((((.........((((((((.((..(....)..)).).))))))).)))))))).))).........)))))))....))))).... (-40.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1039 80 31 171 122 NC_002950:PG0343|5end_methionine_gamma-lyase_378362..378592_NEGATIVE 400 80 31 207 149 NC_002950:PG2003|5end_deoxyguanosinetriphosphate_triphosphohydrolase_2092991..2093221_NEGATIVE 387 178 135 209 167 NC_002950:PG2098|5end_hypothetical_protein_2206635..2206865_NEGATIVE 379 169 124 62 17 NC_002950:PG0165|5end_heat_shock_protein_15_187807..188037_NEGATIVE 366 99 51 141 98 NC_002950:PG0068|5end_hypothetical_protein_81748..81978_POSITIVE 365 80 32 75 14 NC_002950:PG0736|5end_ribonuclease_HII_785608..785838_POSITIVE 363 168 124 221 180 NC_002950:PG0213|5end_precorrin-3_methylase/precorrin-8X_methylmutase_250464..250694_NEGATIVE 362 180 132 124 67 NC_002950:PG0607|5end_hypothetical_protein_662552..662782_POSITIVE 353 99 51 81 34 NC_002950:PG0002|5end_hexapeptide_transferase_family_protein_1295..1525_POSITIVE 352 165 121 214 164 NC_002950:PG1664|5end_ABC_transporter,_permease_protein,_putative_1745813..1746043_POSITIVE 352 100 51 207 143 NC_002950:PG1381|5end_ABC_transporter,_permease_protein_1460622..1460852_POSITIVE 348 99 51 83 30 NC_002950:PG1214|5end_hypothetical_protein_1293032..1293262_POSITIVE 346 90 45 81 21 NC_002950:PG1294|5end_ferrous_iron_transport_protein_B_1372047..1372277_POSITIVE 345 98 52 224 175 NC_002950:PG1621|5end_hypothetical_protein_1701431..1701661_NEGATIVE 345 180 133 101 49 NC_002950:PG1198|5end_hypothetical_protein_1276650..1276880_POSITIVE 335 140 91 159 94 NC_002950:PG0700|5end_hypothetical_protein_756595..756825_NEGATIVE 334 140 93 223 162 NC_002950:PG1042|5end_glycogen_synthase,_putative_1110638..1110868_NEGATIVE 333 179 132 187 151 NC_002950:PG1285|5end_glucosamine-6-phosphate_deaminase_1362894..1363124_POSITIVE 331 168 122 218 161 NC_002950:PG0581|5end_UDP-N-acetylmuramate--L-alanine_ligase_638236..638466_POSITIVE 330 179 132 56 5 NC_002950:PG1162|5end_ATP:cob(I)alamin_adenosyltransferase,_putative_1242039..1242269_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 400 80 31 63 5 NC_002950:PG2004|3end_hypothetical_protein_2093135..2093285_NEGATIVE 379 169 124 56 11 NC_002950:PG0166|3end_peptidyl-tRNA_hydrolase_187813..187963_NEGATIVE 368 69 21 79 35 NC_002950:PG2140|3end_ribosomal_protein_L32_2246399..2246549_POSITIVE 365 80 32 64 3 NC_002950:PG0735|3end_aminotransferase,_class_V_785677..785827_POSITIVE 355 144 102 151 97 NC_002950:PG1364|3end_1-deoxy-D-xylulose_5-phosphate_reductoisomerase_1444388..1444538_NEGATIVE 353 99 51 68 21 NC_002950:PG0001|3end_chromosomal_replication_initiation_protein_1362..1512_POSITIVE 348 180 132 135 82 NC_002950:PG1594|3end_ComEC/Rec2-related_protein_1672052..1672202_NEGATIVE 348 99 51 134 81 NC_002950:PG1213|3end_ribonuclease_H_1293163..1293313_POSITIVE 343 167 123 119 57 NC_002950:PG2099|3end_ATP-dependent_RNA_helicase,_DEAD/DEAH_box_family_2206649..2206799_NEGATIVE 339 169 125 143 96 NC_002950:PG1218|3end_hypothetical_protein_1296475..1296625_POSITIVE 336 180 133 68 7 NC_002950:PG0507|3end_hypothetical_protein_548190..548340_POSITIVE 335 140 91 121 61 NC_002950:PG0333|3end_hypothetical_protein_368811..368961_POSITIVE 325 79 32 145 82 NC_002950:PG0745|3end_lactoylglutathione_lyase,_putative_791982..792132_NEGATIVE 321 172 131 129 78 NC_002950:PG0334|3end_glycosyl_transferase,_group_2_family_protein_368792..368942_NEGATIVE 320 180 131 59 17 NC_002950:PG1133|3end_hypothetical_protein_1214103..1214253_POSITIVE 318 179 132 138 86 NC_002950:PG1648|3end_RelA/SpoT_family_protein_1730082..1730232_POSITIVE 313 137 98 51 6 NC_002950:PG2188|3end_diaminopimelate_decarboxylase_2294633..2294783_NEGATIVE 313 73 33 151 109 NC_002950:PG0857|3end_transcriptional_regulator,_putative_917623..917773_POSITIVE 313 180 133 141 96 NC_002950:PG0738|3end_cytidine/deoxycytidylate_deaminase_family_protein_786995..787145_POSITIVE 313 75 32 147 100 NC_002950:PG0361|3end_hypothetical_protein_395481..395631_POSITIVE