Origin IGS: tgtggggattcttcctcgttggggctttttcgctaaatttgcaggcggacgaggtgctcccctcttcggtgaatttgtaccggggagagctttccctttctatggaatgactttgtaaacgactaaatgaatgatagatatgtgctccgatccttctctttcctgcattcttattcggccggtttcgttcggcctgtgtggttatatgccggcgcgtatggctgatacaagcctgctgtatgagcggtagagcttgagagccgtttcgtaccccttcgaaatagatgaggcacccaccgagttcctgcaggaactcggtgggtgcctctttatttatacctcccctaaaggcctttcgaggtgtccgatttatatcaaatcgggcttacaatctatatatttatcgcaatcgatttatatataaattaaaaatgatttatatacaaaatgaaaacgatatatatacaaatcgaaaaagctttatatataaatcgtgattcgacctttggaaaagcgaatcgcgacctgaaagactcggctttccggtctcctctgctttaggatccgctccctcgcaggtgcttatttggaatgattccaaactcgtcttcgttgttgaaaacttttttcgcacctgttttcagttccgagaacgaccaagcaaaagacagcgaccgtcgatcgggtatataaaagcgaaccccgaaagtccgtcgacggactttcggggttcatattgtcttttgtcaggcctttatgcagcctttggggtgt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 acaccccaaaggctgcataaaggcctgacaaaagacaatatgaaccccgaaagtccgtcgacggactttcggggttcgcttttatatacccgatcgacggtcgctgtcttttgcttggtcgttctcggaactgaaaacaggtgcgaaaaaagttttcaacaacgaagacgagtttggaatcattccaaataagcacctgcgagggagcggatcctaaagcagaggagaccggaaagccgagtctttcaggtcgcgattcgcttttccaaaggtcgaatcacgatttatatataaagctttttcgatttgtatatatatcgttttcattttgtatataaatcatttttaatttatatataaatcgattgcgataaatatatagattgtaagcccgatttgatataaatcggacacctcgaaaggcctttaggggaggtataaataaagaggcacccaccgagttcctgcaggaactcggtgggtgcctcatctatttcgaaggggtacgaaacggctctcaagctctaccgctcatacagcaggcttgtatcagccatacgcgccggcatataaccacacaggccgaacgaaaccggccgaataagaatgcaggaaagagaaggatcggagcacatatctatcattcatttagtcgtttacaaagtcattccatagaaagggaaagctctccccggtacaaattcaccgaagaggggagcacctcgtccgcctgcaaatttagcgaaaaagccccaacgaggaagaatccccaca Mask Tandem Repeat Region ================================================ tgtggggattcttcctcgttggggctttttcgctaaatttgcaggcggacgaggtgctcccctcttcggtgaatttgtaccggggagagctttccctttctatggaatgactttgtaaacgactaaatgaatgatagatatgtgctccgatccttctctttcctgcattcttattcggccggtttcgttcggcctgtgtggttatatgccggcgcgtatggctgatacaagcctgctgtatgagcggtagagcttgagagccgtttcgtaccccttcgaaatagatgaggcacccaccgagttcctgcaggaactcggtgggtgcctctttatttatacctcccctaaaggcctttcgaggtgtccgatttatatcaaatcgggcttacaatctatatatttatcgcaatcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncgtgattcgacctttggaaaagcgaatcgcgacctgaaagactcggctttccggtctcctctgctttaggatccgctccctcgcaggtgcttatttggaatgattccaaactcgtcttcgttgttgaaaacttttttcgcacctgttttcagttccgagaacgaccaagcaaaagacagcgaccgtcgatcgggtatataaaagcgaaccccgaaagtccgtcgacggactttcggggttcatattgtcttttgtcaggcctttatgcagcctttggggtgt Find is-nt database================================================ Query_seq: PG0340:PG0343|PG0340:PG0343:hypothetical protein:methionine gamma-lyase:-><-:376497..377270 774 tgtggggattcttcctcgttggggctttttcgctaaatttgcaggcggacgaggtgctcccctcttcggtgaatttgtaccggggagagctttccctttctatggaatgactttgtaaacgactaaatgaatgatagatatgtgctccgatccttctctttcctgcattcttattcggccggtttcgttcggcctgtgtggttatatgccggcgcgtatggctgatacaagcctgctgtatgagcggtagagcttgagagccgtttcgtaccccttcgaaatagatgaggcacccaccgagttcctgcaggaactcggtgggtgcctctttatttatacctcccctaaaggcctttcgaggtgtccgatttatatcaaatcgggcttacaatctatatatttatcgcaatcgatttatatataaattaaaaatgatttatatacaaaatgaaaacgatatatatacaaatcgaaaaagctttatatataaatcgtgattcgacctttggaaaagcgaatcgcgacctgaaagactcggctttccggtctcctctgctttaggatccgctccctcgcaggtgcttatttggaatgattccaaactcgtcttcgttgttgaaaacttttttcgcacctgttttcagttccgagaacgaccaagcaaaagacagcgaccgtcgatcgggtatataaaagcgaaccccgaaagtccgtcgacggactttcggggttcatattgtcttttgtcaggcctttatgcagcctttggggtgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0340:PG0343|PG0340:PG0343:hypothetical protein:methionine gamma-lyase:-><-:376497..377270 774 tgtggggattcttcctcgttggggctttttcgctaaatttgcaggcggacgaggtgctcccctcttcggtgaatttgtaccggggagagctttccctttctatggaatgactttgtaaacgactaaatgaatgatagatatgtgctccgatccttctctttcctgcattcttattcggccggtttcgttcggcctgtgtggttatatgccggcgcgtatggctgatacaagcctgctgtatgagcggtagagcttgagagccgtttcgtaccccttcgaaatagatgaggcacccaccgagttcctgcaggaactcggtgggtgcctctttatttatacctcccctaaaggcctttcgaggtgtccgatttatatcaaatcgggcttacaatctatatatttatcgcaatcgatttatatataaattaaaaatgatttatatacaaaatgaaaacgatatatatacaaatcgaaaaagctttatatataaatcgtgattcgacctttggaaaagcgaatcgcgacctgaaagactcggctttccggtctcctctgctttaggatccgctccctcgcaggtgcttatttggaatgattccaaactcgtcttcgttgttgaaaacttttttcgcacctgttttcagttccgagaacgaccaagcaaaagacagcgaccgtcgatcgggtatataaaagcgaaccccgaaagtccgtcgacggactttcggggttcatattgtcttttgtcaggcctttatgcagcctttggggtgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0340:PG0343|PG0340:PG0343:hypothetical protein:methionine gamma-lyase:-><-:376497..377270 774 tgtggggattcttcctcgttggggctttttcgctaaatttgcaggcggacgaggtgctcccctcttcggtgaatttgtaccggggagagctttccctttctatggaatgactttgtaaacgactaaatgaatgatagatatgtgctccgatccttctctttcctgcattcttattcggccggtttcgttcggcctgtgtggttatatgccggcgcgtatggctgatacaagcctgctgtatgagcggtagagcttgagagccgtttcgtaccccttcgaaatagatgaggcacccaccgagttcctgcaggaactcggtgggtgcctctttatttatacctcccctaaaggcctttcgaggtgtccgatttatatcaaatcgggcttacaatctatatatttatcgcaatcgatttatatataaattaaaaatgatttatatacaaaatgaaaacgatatatatacaaatcgaaaaagctttatatataaatcgtgattcgacctttggaaaagcgaatcgcgacctgaaagactcggctttccggtctcctctgctttaggatccgctccctcgcaggtgcttatttggaatgattccaaactcgtcttcgttgttgaaaacttttttcgcacctgttttcagttccgagaacgaccaagcaaaagacagcgaccgtcgatcgggtatataaaagcgaaccccgaaagtccgtcgacggactttcggggttcatattgtcttttgtcaggcctttatgcagcctttggggtgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgtggggattcttcctcgttggggctttttcgctaaatttgcaggcggacgaggtgctcccctcttcggtgaatttgtaccggggagagctttccctttctatggaatgactttgtaaacgactaaatgaatgatagatatgtgctccgatccttctctttcctgcattcttattcggccggtttcgttcggcctgtgtggttatatgccggcgcgtatggctgatacaagcctgctgtatgagcggtagagcttgagagccgtttcgtaccccttcgaaatagatgaggcacccaccgagttcctgcaggaactcggtgggtgcctctttatttatacctcccctaaaggcctttcgaggtgtccgatttatatcaaatcgggcttacaatctatatatttatcgcaatcgatttatatataaattaaaaatgatttatatacaaaatgaaaacgatatatatacaaatcgaaaaagctttatatataaatcgtgattcgacctttggaaaagcgaatcgcgacctgaaagactcggctttccggtctcctctgctttaggatccgctccctcgcaggtgcttatttggaatgattccaaactcgtcttcgttgttgaaaacttttttcgcacctgttttcagttccgagaacgaccaagcaaaagacagcgaccgtcgatcgggtatataaaagcgaaccccgaaagtccgtcgacggactttcggggttcatattgtcttttgtcaggcctttatgcagcctttggggtgt Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 2 End: 115 . M G I L P R W G F F A K F A G G R G A P L F G E F V P G R A F P F Y G M T L ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 32 Strand: + Start: 127 End: 222 .............................................................................................................................. M N D R Y V L R S F S F L H S Y S A G F V R P V W L Y A G A Y G ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 39 Strand: + Start: 131 End: 247 .................................................................................................................................. M I D M C S D P S L S C I L I R P V S F G L C G Y M P A R M A D T S L L Y E R ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 39 Strand: + Start: 138 End: 254 ......................................................................................................................................... M C A P I L L F P A F L F G R F R S A C V V I C R R V W L I Q A C C M S G R A ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 56 Strand: + Start: 254 End: 421 ............................................................................................................................................................................................................................................................. M R A V S Y P F E I D E A P T E F L Q E L G G C L F I Y T S P K G L S R C P I Y I K S G L Q S I Y L S Q S I Y I ................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 285 End: 494 ............................................................................................................................................................................................................................................................................................ M R H P P S S C R N S V G A S L F I P P L K A F R G V R F I S N R A Y N L Y I Y R N R F I Y K L K M I Y I Q N E N D I Y ........................................................................................................................................................................................................................................................................................ Protein_Len: 40 Strand: + Start: 439 End: 558 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................... M Y K M K T I Y I Q I E K A L Y I N R D S T F G K A N R D L K D S A F R S P L L ........................................................................................................................................................................................................................ Protein_Len: 33 Strand: + Start: 592 End: 690 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M I P N S S S L L K T F F A P V F S S E N D Q A K D S D R R S G I .................................................................................... Protein_Len: 46 Strand: + Start: 635 End: 772 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M F S V P R T T K Q K T A T V D R V Y K S E P R K S V D G L S G F I L S F V R P L C S L W G .. Protein_Len: 60 Strand: + Start: 579 End: 773 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M L I W N D S K L V F V V E N F F R T C F Q F R E R P S K R Q R P S I G Y I K A N P E S P S T D F R G S Y C L L S G L Y . Protein_Len: 59 Strand: - Start: 586 End: 762 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... K S H N W V R R R Q Q F S K E C R N E T G L V V L C F V A V T S R T Y L L S G R F D T S P S E P N M N D K T L G K H M ............ Protein_Len: 43 Strand: - Start: 561 End: 689 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ S G S G R L H K N P I I G F E D E N N F V K K A G T K L E S F S W A F S L S R R D P M ..................................................................................... Protein_Len: 60 Strand: - Start: 440 End: 634 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................... S I Y V F R F L K I Y L D H N S R Q F L S D R G S L S P K G T E E A K P D A G E C T S I Q F S E L S T K T T S F K K R M ............................................................................................................................................ Protein_Len: 43 Strand: - Start: 205 End: 333 ............................................................................................................................................................................................................ I G A R I A S V L R S Y S R Y L K L A T E Y G K S I S S A G V S N R C S S P P H R K M ......................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 40 Strand: - Start: 174 End: 293 ............................................................................................................................................................................. E A P K T R G T H N Y A P A Y P Q Y L G A T H A T S S S L R K T G R R F L H P M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 2 End: 373 . Q T T I H R R T H S I C A Q Q I L P L A Q S G N R V G E F Y I L C G G L E Q L F E T P A E K N I G G R F A K R P T R N M ................................................................................................................................................................................................................................................................................................................................................................................................................. tgtggggattcttcctcgttggggctttttcgctaaatttgcaggcggacgaggtgctcccctcttcggtgaatttgtaccggggagagctttccctttctatggaatgactttgtaaacgactaaatgaatgatagatatgtgctccgatccttctctttcctgcattcttattcggccggtttcgttcggcctgtgtggttatatgccggcgcgtatggctgatacaagcctgctgtatgagcggtagagcttgagagccgtttcgtaccccttcgaaatagatgaggcacccaccgagttcctgcaggaactcggtgggtgcctctttatttatacctcccctaaaggcctttcgaggtgtccgatttatatcaaatcgggcttacaatctatatatttatcgcaatcgatttatatataaattaaaaatgatttatatacaaaatgaaaacgatatatatacaaatcgaaaaagctttatatataaatcgtgattcgacctttggaaaagcgaatcgcgacctgaaagactcggctttccggtctcctctgctttaggatccgctccctcgcaggtgcttatttggaatgattccaaactcgtcttcgttgttgaaaacttttttcgcacctgttttcagttccgagaacgaccaagcaaaagacagcgaccgtcgatcgggtatataaaagcgaaccccgaaagtccgtcgacggactttcggggttcatattgtcttttgtcaggcctttatgcagcctttggggtgt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgtggggattcttcctcgttggggctttttcgctaaatttgcaggcggacgaggtgctcccctcttcggtgaatttgtaccggggagagctttccctttctatggaatgactttgtaaacgactaaatgaatgatagatatgtgctccgatccttctctttcctgcattcttattcggccggtttcgttcggcctgtgtggttatatgccggcgcgtatggctgatacaagcctgctgtatgagcggtagagcttgagagccgtttcgtaccccttcgaaatagatgaggcacccaccgagttcctgcaggaactcggtgggtgcctctttatttatacctcccctaaaggcctttcgaggtgtccgatttatatcaaatcgggcttacaatctatatatttatcgcaatcgatttatatataaattaaaaatgatttatatacaaaatgaaaacgatatatatacaaatcgaaaaagctttatatataaatcgtgattcgacctttggaaaagcgaatcgcgacctgaaagactcggctttccggtctcctctgctttaggatccgctccctcgcaggtgcttatttggaatgattccaaactcgtcttcgttgttgaaaacttttttcgcacctgttttcagttccgagaacgaccaagcaaaagacagcgaccgtcgatcgggtatataaaagcgaaccccgaaagtccgtcgacggactttcggggttcatattgtcttttgtcaggcctttatgcagcctttggggtgt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 42 HP_score: -3.0 Tail_Score: -2.87931 Start: 208 End: 236 Full_Region: CCTGTGTGGTTATAT GCCGGCGCGTAT GGCTG ATACAAGCCTGC TGTATGAGCGGTAGA ...............................................................................................................................................................................................................GCCGGCGCGTATGGCTGATACAAGCCTGC.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -26.5 Tail_Score: -3.53811 Start: 282 End: 332 Full_Region: taggggaggtataaa taaagaggcacccaccgagttcc tgca ggaactcggtgggtgcctcatcta tttcgaaggggtacg .........................................................................................................................................................................................................................................................................................taaagaggcacccaccgagttcctgcaggaactcggtgggtgcctcatcta.......................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -27.2 Tail_Score: -3.34715 Start: 284 End: 331 Full_Region: aggggaggtataaat aaagaggcacccaccgagttcc tgca ggaactcggtgggtgcctcatc tatttcgaaggggta ...........................................................................................................................................................................................................................................................................................aaagaggcacccaccgagttcctgcaggaactcggtgggtgcctcatc........................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -29.8 Tail_Score: -3.24232 Start: 286 End: 329 Full_Region: gggaggtataaataa agaggcacccaccgagttcc tgca ggaactcggtgggtgcctca tctatttcgaagggg .............................................................................................................................................................................................................................................................................................agaggcacccaccgagttcctgcaggaactcggtgggtgcctca............................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 100 HP_score: -33.3 Tail_Score: -4.57482 Start: 287 End: 328 Full_Region: CCCTTCGAAATAGAT GAGGCACCCACCGAGTTCC TGCA GGAACTCGGTGGGTGCCTC TTTATTTATACCTCC ..............................................................................................................................................................................................................................................................................................GAGGCACCCACCGAGTTCCTGCAGGAACTCGGTGGGTGCCTC.............................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 100 HP_score: -33.3 Tail_Score: -2.54325 Start: 287 End: 328 Full_Region: ggaggtataaataaa gaggcacccaccgagttcc tgca ggaactcggtgggtgcctc atctatttcgaaggg ..............................................................................................................................................................................................................................................................................................gaggcacccaccgagttcctgcaggaactcggtgggtgcctc.............................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 100 HP_score: -30.1 Tail_Score: -2.80821 Start: 289 End: 326 Full_Region: aggtataaataaaga ggcacccaccgagttcc tgca ggaactcggtgggtgcc tcatctatttcgaag ................................................................................................................................................................................................................................................................................................ggcacccaccgagttcctgcaggaactcggtgggtgcc................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 40 HP_score: -2.3 Tail_Score: -2.74514 Start: 455 End: 494 Full_Region: TATACAAAATGAAAA CGATATATATACAAATC GAAAAA GCTTTATATATAAATCG TGATTCGACCTTTGG ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................CGATATATATACAAATCGAAAAAGCTTTATATATAAATCG........................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 73 HP_score: -4.5 Tail_Score: -4.75518 Start: 455 End: 494 Full_Region: ccaaaggtcgaatca cgatttatatataaagc tttttc gatttgtatatatatcg ttttcattttgtata ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................cgatttatatataaagctttttcgatttgtatatatatcg........................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 43 HP_score: -2.9 Tail_Score: -3.01497 Start: 520 End: 551 Full_Region: TTTGGAAAAGCGAAT CGCGACCTGAAAG ACTCGG CTTTCCGGTCTCC TCTGCTTTAGGATCC .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CGCGACCTGAAAGACTCGGCTTTCCGGTCTCC............................................................................................................................................................................................................................... TransTerm Strand: + Conf: 58 HP_score: -7.6 Tail_Score: -2.7314 Start: 523 End: 548 Full_Region: GGAAAAGCGAATCGC GACCTGAAAG ACTCGG CTTTCCGGTC TCCTCTGCTTTAGGA ..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GACCTGAAAGACTCGGCTTTCCGGTC.................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 48 HP_score: -4.4 Tail_Score: -2.90725 Start: 525 End: 546 Full_Region: AAAAGCGAATCGCGA CCTGAAAG ACTCGG CTTTCCGG TCTCCTCTGCTTTAG ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CCTGAAAGACTCGGCTTTCCGG.................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 72 HP_score: -5.5 Tail_Score: -4.47217 Start: 667 End: 679 Full_Region: tttatatacccgatc gacgg tcg ctgtc ttttgcttggtcgtt ..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gacggtcgctgtc............................................................................................... TransTerm Strand: + Conf: 95 HP_score: -12.2 Tail_Score: -4.39002 Start: 689 End: 741 Full_Region: ACCGTCGATCGGGTA TATAAAAGCGAACCCCGAAAGTCCG TCGA CGGACTTTCGGGGTTCATATTGTC TTTTGTCAGGCCTTT ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TATAAAAGCGAACCCCGAAAGTCCGTCGACGGACTTTCGGGGTTCATATTGTC................................. TransTerm Strand: + Conf: 100 HP_score: -18.1 Tail_Score: -4.02581 Start: 695 End: 736 Full_Region: GATCGGGTATATAAA AGCGAACCCCGAAAGTCCG TCGA CGGACTTTCGGGGTTCATA TTGTCTTTTGTCAGG ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................AGCGAACCCCGAAAGTCCGTCGACGGACTTTCGGGGTTCATA...................................... TransTerm Strand: - Conf: 100 HP_score: -21.6 Tail_Score: -4.52857 Start: 696 End: 735 Full_Region: ctgacaaaagacaat atgaaccccgaaagtccg tcga cggactttcggggttcgc ttttatatacccgat .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................atgaaccccgaaagtccgtcgacggactttcggggttcgc....................................... TransTerm Strand: - Conf: 100 HP_score: -25.1 Tail_Score: -3.31232 Start: 697 End: 734 Full_Region: tgacaaaagacaata tgaaccccgaaagtccg tcga cggactttcggggttcg cttttatatacccga ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgaaccccgaaagtccgtcgacggactttcggggttcg........................................ TransTerm Strand: + Conf: 100 HP_score: -22.9 Tail_Score: -3.59658 Start: 697 End: 734 Full_Region: TCGGGTATATAAAAG CGAACCCCGAAAGTCCG TCGA CGGACTTTCGGGGTTCA TATTGTCTTTTGTCA ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CGAACCCCGAAAGTCCGTCGACGGACTTTCGGGGTTCA........................................ TransTerm Strand: - Conf: 100 HP_score: -26.4 Tail_Score: -2.58418 Start: 698 End: 733 Full_Region: gacaaaagacaatat gaaccccgaaagtccg tcga cggactttcggggttc gcttttatatacccg .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gaaccccgaaagtccgtcgacggactttcggggttc......................................... TransTerm Strand: + Conf: 100 HP_score: -26.4 Tail_Score: -2.7471 Start: 698 End: 733 Full_Region: CGGGTATATAAAAGC GAACCCCGAAAGTCCG TCGA CGGACTTTCGGGGTTC ATATTGTCTTTTGTC .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GAACCCCGAAAGTCCGTCGACGGACTTTCGGGGTTC......................................... TransTerm Strand: - Conf: 100 HP_score: -22.3 Tail_Score: -3.43774 Start: 701 End: 730 Full_Region: aaaagacaatatgaa ccccgaaagtccg tcga cggactttcgggg ttcgcttttatatac ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ccccgaaagtccgtcgacggactttcgggg............................................ Find igs database================================================ Query_seq: PG0340:PG0343|PG0340:PG0343:hypothetical protein:methionine gamma-lyase:-><-:376497..377270 774 tgtggggattcttcctcgttggggctttttcgctaaatttgcaggcggacgaggtgctcccctcttcggtgaatttgtaccggggagagctttccctttctatggaatgactttgtaaacgactaaatgaatgatagatatgtgctccgatccttctctttcctgcattcttattcggccggtttcgttcggcctgtgtggttatatgccggcgcgtatggctgatacaagcctgctgtatgagcggtagagcttgagagccgtttcgtaccccttcgaaatagatgaggcacccaccgagttcctgcaggaactcggtgggtgcctctttatttatacctcccctaaaggcctttcgaggtgtccgatttatatcaaatcgggcttacaatctatatatttatcgcaatcgatttatatataaattaaaaatgatttatatacaaaatgaaaacgatatatatacaaatcgaaaaagctttatatataaatcgtgattcgacctttggaaaagcgaatcgcgacctgaaagactcggctttccggtctcctctgctttaggatccgctccctcgcaggtgcttatttggaatgattccaaactcgtcttcgttgttgaaaacttttttcgcacctgttttcagttccgagaacgaccaagcaaaagacagcgaccgtcgatcgggtatataaaagcgaaccccgaaagtccgtcgacggactttcggggttcatattgtcttttgtcaggcctttatgcagcctttggggtgt Intra-Species Hit: Count: 1 Min: 1 Max: 774 Len: 774 Subject: NC_002950_PG0340_PG0343|hypothetical protein:methionine gamma-lyase|POSITIVE:NEGATIVE|[376497,377270]|774 HSP 1 e-value: 0.0 bit: 823.0 Len: 415 Query Start:1 Query End:415 Subject Strand: POSITIVE Subject Start: 1 Subject End: 415 tgtggggattcttcctcgttggggctttttcgctaaatttgcaggcggacgaggtgctcccctcttcggtgaatttgtaccggggagagctttccctttctatggaatgactttgtaaacgactaaatgaatgatagatatgtgctccgatccttctctttcctgcattcttattcggccggtttcgttcggcctgtgtggttatatgccggcgcgtatggctgatacaagcctgctgtatgagcggtagagcttgagagccgtttcgtaccccttcgaaatagatgaggcacccaccgagttcctgcaggaactcggtgggtgcctctttatttatacctcccctaaaggcctttcgaggtgtccgatttatatcaaatcgggcttacaatctatatatttatcgcaatcgatt....................................................................................................................................................................................................................................................................................................................................................................... tgtggggattcttcctcgttggggctttttcgctaaatttgcaggcggacgaggtgctcccctcttcggtgaatttgtaccggggagagctttccctttctatggaatgactttgtaaacgactaaatgaatgatagatatgtgctccgatccttctctttcctgcattcttattcggccggtttcgttcggcctgtgtggttatatgccggcgcgtatggctgatacaagcctgctgtatgagcggtagagcttgagagccgtttcgtaccccttcgaaatagatgaggcacccaccgagttcctgcaggaactcggtgggtgcctctttatttatacctcccctaaaggcctttcgaggtgtccgatttatatcaaatcgggcttacaatctatatatttatcgcaatcgatt....................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-158 bit: 559.0 Len: 282 Query Start:493 Query End:774 Subject Strand: POSITIVE Subject Start: 493 Subject End: 774 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cgtgattcgacctttggaaaagcgaatcgcgacctgaaagactcggctttccggtctcctctgctttaggatccgctccctcgcaggtgcttatttggaatgattccaaactcgtcttcgttgttgaaaacttttttcgcacctgttttcagttccgagaacgaccaagcaaaagacagcgaccgtcgatcgggtatataaaagcgaaccccgaaagtccgtcgacggactttcggggttcatattgtcttttgtcaggcctttatgcagcctttggggtgt ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cgtgattcgacctttggaaaagcgaatcgcgacctgaaagactcggctttccggtctcctctgctttaggatccgctccctcgcaggtgcttatttggaatgattccaaactcgtcttcgttgttgaaaacttttttcgcacctgttttcagttccgagaacgaccaagcaaaagacagcgaccgtcgatcgggtatataaaagcgaaccccgaaagtccgtcgacggactttcggggttcatattgtcttttgtcaggcctttatgcagcctttggggtgt HSP 3 e-value: 8.0E-15 bit: 83.8 Len: 42 Query Start:287 Query End:328 Subject Strand: NEGATIVE Subject Start: 287 Subject End: 328 ..............................................................................................................................................................................................................................................................................................gaggcacccaccgagttcctgcaggaactcggtgggtgcctc.............................................................................................................................................................................................................................................................................................................................................................................................................................................................. ..............................................................................................................................................................................................................................................................................................gaggcacccaccgagttcctgcaggaactcggtgggtgcctc.............................................................................................................................................................................................................................................................................................................................................................................................................................................................. HSP 4 e-value: 3.0E-11 bit: 71.9 Len: 36 Query Start:698 Query End:733 Subject Strand: NEGATIVE Subject Start: 698 Subject End: 733 .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gaaccccgaaagtccgtcgacggactttcggggttc......................................... .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gaaccccgaaagtccgtcgacggactttcggggttc......................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUGGGGAUUCUUCCUCGUUGGGGCUUUUUCGCUAAAUUUGCAGGCGGACGAGGUGCUCCCCUCUUCGGUGAAUUUGUACCGGGGAGAGCUUUCCCUUUCUAUGGAAUGACUUUGUAAACGACUAAAUGAAUGAUAGAUAUGUGCUCCGAUCCUUCUCUUUCCUGCAUUCUUAUUCGGCCGGUUUCGUUCGGCCUGUGUGGUUAUAUGCCGGCGCGUAUGGCUGAUACAAGCCUGCUGUAUGAGCGGUAGAGCUUGAGAGCCGUUUCGUACCCCUUCGAAAUAGAUGAGGCACCCACCGAGUUCCUGCAGGAACUCGGUGGGUGCCUCUUUAUUUAUACCUCCCCUAAAGGCCUUUCGAGGUGUCCGAUUUAUAUCAAAUCGGGCUUACAAUCUAUAUAUUUAUCGCAAUCGAUUUAUAUAUAAAUUAAAAAUGAUUUAUAUACAAAAUGAAAACGAUAUAUAUACAAAUCGAAAAAGCUUUAUAUAUAAAUCGUGAUUCGACCUUUGGAAAAGCGAAUCGCGACCUGAAAGACUCGGCUUUCCGGUCUCCUCUGCUUUAGGAUCCGCUCCCUCGCAGGUGCUUAUUUGGAAUGAUUCCAAACUCGUCUUCGUUGUUGAAAACUUUUUUCGCACCUGUUUUCAGUUCCGAGAACGACCAAGCAAAAGACAGCGACCGUCGAUCGGGUAUAUAAAAGCGAACCCCGAAAGUCCGUCGACGGACUUUCGGGGUUCAUAUUGUCUUUUGUCAGGCCUUUAUGCAGCCUUUGGGGUGU ((..((((.........((((((((.......(((.(((((..((((.(((((((((((...(((((((((......))))))))))))).((((........))))..)))))))...)).))...)))))..)))......))))))))........))))..)).........((((((......)))))).....(((........((..((.((((((..(.((((((((((((....))))))).)))))).))))))..))..))......(((((((.(((((((((((((((((((....))))))))))))))))))))))))))..)))..((((((((((.((((((...(((((((((.....)))))))))..........(((((.(((((...(((.(((.(((((((((((....))))))))))).))).)))...))))).)))))....))))))..((............((((((((((....((....)).))))))))))((((.....(((((.....(((..((((.......)))).))).......((((((((...((((((.....))))))...((.((((....)))).)).......))))))))........))))).........(((((((((((...(((.....)))............((((((((((((((((....))))))))))))))))...))))))))))))))).......)).))))))))))... (-288.05) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACACCCCAAAGGCUGCAUAAAGGCCUGACAAAAGACAAUAUGAACCCCGAAAGUCCGUCGACGGACUUUCGGGGUUCGCUUUUAUAUACCCGAUCGACGGUCGCUGUCUUUUGCUUGGUCGUUCUCGGAACUGAAAACAGGUGCGAAAAAAGUUUUCAACAACGAAGACGAGUUUGGAAUCAUUCCAAAUAAGCACCUGCGAGGGAGCGGAUCCUAAAGCAGAGGAGACCGGAAAGCCGAGUCUUUCAGGUCGCGAUUCGCUUUUCCAAAGGUCGAAUCACGAUUUAUAUAUAAAGCUUUUUCGAUUUGUAUAUAUAUCGUUUUCAUUUUGUAUAUAAAUCAUUUUUAAUUUAUAUAUAAAUCGAUUGCGAUAAAUAUAUAGAUUGUAAGCCCGAUUUGAUAUAAAUCGGACACCUCGAAAGGCCUUUAGGGGAGGUAUAAAUAAAGAGGCACCCACCGAGUUCCUGCAGGAACUCGGUGGGUGCCUCAUCUAUUUCGAAGGGGUACGAAACGGCUCUCAAGCUCUACCGCUCAUACAGCAGGCUUGUAUCAGCCAUACGCGCCGGCAUAUAACCACACAGGCCGAACGAAACCGGCCGAAUAAGAAUGCAGGAAAGAGAAGGAUCGGAGCACAUAUCUAUCAUUCAUUUAGUCGUUUACAAAGUCAUUCCAUAGAAAGGGAAAGCUCUCCCCGGUACAAAUUCACCGAAGAGGGGAGCACCUCGUCCGCCUGCAAAUUUAGCGAAAAAGCCCCAACGAGGAAGAAUCCCCACA ...(((((((((((.(.....(((((((...(((((.....((((((((((((((((....))))))))))))))))((((((..............(((((....((((((((((((((((((((...........(((((((.......((((((......))))))..(((((((.....)))))))..)))))))...))))))))..))..)))))))))))))))))))))...))))))))))))..((((((((((....)))).)))))).((((((((((.((((((((...((((((((((((.((((((..((..(((((((((((((........)))))))))))))..))..)))))).)))))))))))).))))....))))))))))))))........)...))))))).)))).((..........(((((((((((((((((((....))))))))))))))))))).(((.(((((..(((((.......((((..(((((.((...(((.....))))))))))....))))...(((...((.......)).....(((((........))))).........((((((...(((...(((.(((.......)))))).........................((((........)))).((((((((((((........))))..).)))))))..))).....))))))......))).....)))))..))))).)))....))... (-297.37) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 658 629 581 119 71 NC_002950:PG0609|5end_hypothetical_protein_663183..663413_POSITIVE 559 629 581 52 4 NC_002950:PG0995|5end_hypothetical_protein_1055585..1055815_NEGATIVE 557 629 581 82 34 NC_002950:PG1357|5end_hypothetical_protein_1436458..1436688_NEGATIVE 543 629 582 64 17 NC_002950:PG0773|5end_hypothetical_protein_825131..825361_POSITIVE 494 60 16 74 23 NC_002950:PG0059|5end_hypothetical_protein_71526..71756_POSITIVE 469 214 173 191 144 NC_002950:PG1021|5end_hypothetical_protein_1083592..1083822_NEGATIVE 466 330 290 42 1 NC_002950:PG0371|5end_hypothetical_protein_404043..404273_POSITIVE 457 330 285 81 36 NC_002950:PG1223|5end_hypothetical_protein_1299461..1299691_POSITIVE 456 214 172 199 143 NC_002950:PG1997|5end_hypothetical_protein_2086831..2087061_POSITIVE 450 90 41 47 5 NC_002950:PG1351|5end_hypothetical_protein_1432744..1432974_NEGATIVE 439 278 231 118 65 NC_002950:PG1273|5end_hypothetical_protein_1351837..1352067_NEGATIVE 436 240 191 51 8 NC_002950:PG1118|5end_clpB_protein_1194674..1194904_NEGATIVE 435 269 221 91 41 NC_002950:PG2072|5end_UvrD/REP_helicase_domain_protein_2167369..2167599_POSITIVE 431 330 286 102 54 NC_002950:PG1256|5end_ribonuclease,_Rne/Rng_family_1334262..1334492_NEGATIVE 429 218 175 108 52 NC_002950:PG2146|5end_hypothetical_protein_2253815..2254045_POSITIVE 426 90 43 173 128 NC_002950:PG0736|5end_ribonuclease_HII_785608..785838_POSITIVE 422 86 43 111 59 NC_002950:PG0608|5end_hypothetical_protein_662945..663175_POSITIVE 419 768 722 198 153 NC_002950:PG1934|5end_30S_ribosomal_protein_S19_2022345..2022575_NEGATIVE 419 249 204 125 62 NC_002950:PG0326|5end_hypothetical_protein_360974..361204_NEGATIVE 417 218 177 73 22 NC_002950:PG2156|5end_conserved_hypothetical_protein_TIGR00046_2262645..2262875_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 742 628 581 52 5 NC_002950:PG1868|3end_hypothetical_protein_1965098..1965248_NEGATIVE 576 330 283 58 8 NC_002950:PG1274|3end_elongation_factor_P_1352489..1352639_POSITIVE 543 629 582 57 10 NC_002950:PG0772|3end_hypothetical_protein_825204..825354_POSITIVE 506 330 283 54 8 NC_002950:PG0568|3end_elongation_factor_P_623999..624149_NEGATIVE 494 60 16 60 9 NC_002950:PG0058|3end_nicotinic_acid_mononucleotide_adenyltransferase_71592..71742_POSITIVE 486 618 582 151 115 NC_002950:PG1167|3end_hypothetical_protein_1248644..1248794_POSITIVE 478 610 564 149 95 NC_002950:PG0995|3end_hypothetical_protein_1055469..1055619_NEGATIVE 473 750 701 39 1 NC_002950:PG0046|3end_phosphatidate_cytidylyltransferase_56346..56496_NEGATIVE 466 330 290 143 102 NC_002950:PG0371|3end_hypothetical_protein_404224..404374_POSITIVE 463 732 692 72 27 NC_002950:PG1678|3end_hypothetical_protein_1763755..1763905_NEGATIVE 452 90 44 74 12 NC_002950:PG1213|3end_ribonuclease_H_1293163..1293313_POSITIVE 450 90 41 54 12 NC_002950:PG1352|3end_hypothetical_protein_1432737..1432887_NEGATIVE 442 476 431 48 3 NC_002950:PG0773|3end_hypothetical_protein_825348..825498_POSITIVE 435 269 221 94 44 NC_002950:PG2071|3end_hypothetical_protein_2167452..2167602_POSITIVE 429 218 175 151 95 NC_002950:PG2145|3end_polysaccharide_deacetylase_2253938..2254088_POSITIVE 425 237 198 57 4 NC_002950:PG1247|3end_3-dehydroquinate_synthase_1325585..1325735_POSITIVE 422 86 43 80 28 NC_002950:PG0607|3end_hypothetical_protein_662994..663144_POSITIVE 421 530 481 117 65 NC_002950:PG1357|3end_hypothetical_protein_1436327..1436477_NEGATIVE 412 220 174 76 26 NC_002950:PG1297|3end_ribosomal_protein_S1_1377710..1377860_POSITIVE 407 557 513 59 15 NC_002950:PG1410|3end_hypothetical_protein_1493436..1493586_POSITIVE