Origin IGS: aacttgcttattattagtttcttgttatttgatgaccactcatacccgatacatccctcaagtcctccgaaaaaaaacgtctggacgttcggaggaaaacgtctagacgttccgaataaaacgtctagacgtttcgagcaaaaggtctagacgtttcgggcaaaacgtctagacgttttttcgaggggatattgcagacggccacggccacgtcccggcgtttcccgggggaggctctcggccttcctcccgtgaggtgcgtcggctcctcccgcacgcggctgaccgagggggcggccacgacttgcgggggcggtctgcaaaccgcggcaaaggtacccaaatagttcgattcggcagacagtaaaataaatactcttgtcgtgtcgtttatggagacgaaggggggcggcgtcccgctctttgcgatcgactctccgcatcgcccccgtgggccgtagcatccgcaggcgcagccggcctcggcgaataaggcggagggagtaagaccatcattcgatttatttcttacatttgcacgacggaaaacgaaatagacacctattctctatcctttcgtcctgcggtatcgagtatcctgacagtctcattccgagatgccgtatccccctatcaacacttatcatatttctaaaaagctcatcatcatttatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aataaatgatgatgagctttttagaaatatgataagtgttgatagggggatacggcatctcggaatgagactgtcaggatactcgataccgcaggacgaaaggatagagaataggtgtctatttcgttttccgtcgtgcaaatgtaagaaataaatcgaatgatggtcttactccctccgccttattcgccgaggccggctgcgcctgcggatgctacggcccacgggggcgatgcggagagtcgatcgcaaagagcgggacgccgccccccttcgtctccataaacgacacgacaagagtatttattttactgtctgccgaatcgaactatttgggtacctttgccgcggtttgcagaccgcccccgcaagtcgtggccgccccctcggtcagccgcgtgcgggaggagccgacgcacctcacgggaggaaggccgagagcctcccccgggaaacgccgggacgtggccgtggccgtctgcaatatcccctcgaaaaaacgtctagacgttttgcccgaaacgtctagaccttttgctcgaaacgtctagacgttttattcggaacgtctagacgttttcctccgaacgtccagacgttttttttcggaggacttgagggatgtatcgggtatgagtggtcatcaaataacaagaaactaataataagcaagtt Mask Tandem Repeat Region ================================================ aacttgcttattattagtttcttgttatttgatgaccactcatacccgatacatccctcaagtcctccgaaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntttcgaggggatattgcagacggccacggccacgtcccggcgtttcccgggggaggctctcggccttcctcccgtgaggtgcgtcggctcctcccgcacgcggctgaccgagggggcggccacgacttgcgggggcggtctgcaaaccgcggcaaaggtacccaaatagttcgattcggcagacagtaaaataaatactcttgtcgtgtcgtttatggagacgaaggggggcggcgtcccgctctttgcgatcgactctccgcatcgcccccgtgggccgtagcatccgcaggcgcagccggcctcggcgaataaggcggagggagtaagaccatcattcgatttatttcttacatttgcacgacggaaaacgaaatagacacctattctctatcctttcgtcctgcggtatcgagtatcctgacagtctcattccgagatgccgtatccccctatcaacacttatcatatttctaaaaagctcatcatcatttatt Find is-nt database================================================ Query_seq: PG0330:PG0332|PG0330:PG0332:DNA-binding protein, histone-like family:transcription termination factor Rho:<-->:365084..365758 675 aacttgcttattattagtttcttgttatttgatgaccactcatacccgatacatccctcaagtcctccgaaaaaaaacgtctggacgttcggaggaaaacgtctagacgttccgaataaaacgtctagacgtttcgagcaaaaggtctagacgtttcgggcaaaacgtctagacgttttttcgaggggatattgcagacggccacggccacgtcccggcgtttcccgggggaggctctcggccttcctcccgtgaggtgcgtcggctcctcccgcacgcggctgaccgagggggcggccacgacttgcgggggcggtctgcaaaccgcggcaaaggtacccaaatagttcgattcggcagacagtaaaataaatactcttgtcgtgtcgtttatggagacgaaggggggcggcgtcccgctctttgcgatcgactctccgcatcgcccccgtgggccgtagcatccgcaggcgcagccggcctcggcgaataaggcggagggagtaagaccatcattcgatttatttcttacatttgcacgacggaaaacgaaatagacacctattctctatcctttcgtcctgcggtatcgagtatcctgacagtctcattccgagatgccgtatccccctatcaacacttatcatatttctaaaaagctcatcatcatttatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0330:PG0332|PG0330:PG0332:DNA-binding protein, histone-like family:transcription termination factor Rho:<-->:365084..365758 675 aacttgcttattattagtttcttgttatttgatgaccactcatacccgatacatccctcaagtcctccgaaaaaaaacgtctggacgttcggaggaaaacgtctagacgttccgaataaaacgtctagacgtttcgagcaaaaggtctagacgtttcgggcaaaacgtctagacgttttttcgaggggatattgcagacggccacggccacgtcccggcgtttcccgggggaggctctcggccttcctcccgtgaggtgcgtcggctcctcccgcacgcggctgaccgagggggcggccacgacttgcgggggcggtctgcaaaccgcggcaaaggtacccaaatagttcgattcggcagacagtaaaataaatactcttgtcgtgtcgtttatggagacgaaggggggcggcgtcccgctctttgcgatcgactctccgcatcgcccccgtgggccgtagcatccgcaggcgcagccggcctcggcgaataaggcggagggagtaagaccatcattcgatttatttcttacatttgcacgacggaaaacgaaatagacacctattctctatcctttcgtcctgcggtatcgagtatcctgacagtctcattccgagatgccgtatccccctatcaacacttatcatatttctaaaaagctcatcatcatttatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0330:PG0332|PG0330:PG0332:DNA-binding protein, histone-like family:transcription termination factor Rho:<-->:365084..365758 675 aacttgcttattattagtttcttgttatttgatgaccactcatacccgatacatccctcaagtcctccgaaaaaaaacgtctggacgttcggaggaaaacgtctagacgttccgaataaaacgtctagacgtttcgagcaaaaggtctagacgtttcgggcaaaacgtctagacgttttttcgaggggatattgcagacggccacggccacgtcccggcgtttcccgggggaggctctcggccttcctcccgtgaggtgcgtcggctcctcccgcacgcggctgaccgagggggcggccacgacttgcgggggcggtctgcaaaccgcggcaaaggtacccaaatagttcgattcggcagacagtaaaataaatactcttgtcgtgtcgtttatggagacgaaggggggcggcgtcccgctctttgcgatcgactctccgcatcgcccccgtgggccgtagcatccgcaggcgcagccggcctcggcgaataaggcggagggagtaagaccatcattcgatttatttcttacatttgcacgacggaaaacgaaatagacacctattctctatcctttcgtcctgcggtatcgagtatcctgacagtctcattccgagatgccgtatccccctatcaacacttatcatatttctaaaaagctcatcatcatttatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aacttgcttattattagtttcttgttatttgatgaccactcatacccgatacatccctcaagtcctccgaaaaaaaacgtctggacgttcggaggaaaacgtctagacgttccgaataaaacgtctagacgtttcgagcaaaaggtctagacgtttcgggcaaaacgtctagacgttttttcgaggggatattgcagacggccacggccacgtcccggcgtttcccgggggaggctctcggccttcctcccgtgaggtgcgtcggctcctcccgcacgcggctgaccgagggggcggccacgacttgcgggggcggtctgcaaaccgcggcaaaggtacccaaatagttcgattcggcagacagtaaaataaatactcttgtcgtgtcgtttatggagacgaaggggggcggcgtcccgctctttgcgatcgactctccgcatcgcccccgtgggccgtagcatccgcaggcgcagccggcctcggcgaataaggcggagggagtaagaccatcattcgatttatttcttacatttgcacgacggaaaacgaaatagacacctattctctatcctttcgtcctgcggtatcgagtatcctgacagtctcattccgagatgccgtatccccctatcaacacttatcatatttctaaaaagctcatcatcatttatt Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: + Start: 4 End: 147 ... M L I I S F L L F D D H S Y P I H P S S P P K K N V W T F G G K R L D V P N K T S R R F E Q K V ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 52 Strand: + Start: 189 End: 344 ............................................................................................................................................................................................ M L Q T A T A T S R R F P G E A L G L P P V R C V G S S R T R L T E G A A T T C G G G L Q T A A K V P K ........................................................................................................................................................................................................................................................................................................................................... Protein_Len: 58 Strand: + Start: 191 End: 364 .............................................................................................................................................................................................. M A D G H G H V P A F P G G G S R P S S R E V R R L L P H A A D R G G G H D L R G R S A N R G K G T Q I V R F G R Q ....................................................................................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: + Start: 369 End: 458 ................................................................................................................................................................................................................................................................................................................................................................................ M N T L V V S F M E T K G G G V P L F A I D S P H R P R G P ......................................................................................................................................................................................................................... Protein_Len: 44 Strand: + Start: 373 End: 504 .................................................................................................................................................................................................................................................................................................................................................................................... M L L S C R L W R R R G A A S R S L R S T L R I A P V G R S I R R R S R P R R I R R R E ........................................................................................................................................................................... Protein_Len: 47 Strand: + Start: 512 End: 652 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M I R F I S Y I C T T E N E I D T Y S L S F R P A V S S I L T V S F R D A V S P Y Q H L S Y F ....................... Protein_Len: 52 Strand: + Start: 520 End: 675 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M Y F L H L H D G K R N R H L F S I L S S C G I E Y P D S L I P R C R I P L S T L I I F L K S S S S F I Protein_Len: 50 Strand: - Start: 525 End: 674 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ K K C K C S P F R F L C R N E I R E D Q P I S Y G S L R M G L H R I G R D V S I M N R F L E D D N M . Protein_Len: 44 Strand: - Start: 394 End: 525 ......................................................................................................................................................................................................................................................................................................................................................................................................... P S S P P R R G A R Q S R S E A D G G H A T A D A P A A P R P S Y P P P L L V M M R N M ...................................................................................................................................................... Protein_Len: 60 Strand: - Start: 172 End: 360 ........................................................................................................................................................................... R P S I A S P W P W T G A N G P P P E R G E E R S T R R S R G C A A S R P P P W S K R P R D A F R P L P V W I T R N P M ........................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 150 End: 491 ..................................................................................................................................................... A T Q L G R C L Y G F L E I R C V T F Y I S K D H R K H L R L P A A D R E K R D V R R M A G T P R L M R L R L R G R R M ........................................................................................................................................................................................ Protein_Len: 60 Strand: - Start: 128 End: 565 ............................................................................................................................... D N I S V F P P P T G S K A I S E G C R G R P G Y C G C A C G A E A F L A S P T L G D N S K N R V N A R R F V F Y V G M .............................................................................................................. aacttgcttattattagtttcttgttatttgatgaccactcatacccgatacatccctcaagtcctccgaaaaaaaacgtctggacgttcggaggaaaacgtctagacgttccgaataaaacgtctagacgtttcgagcaaaaggtctagacgtttcgggcaaaacgtctagacgttttttcgaggggatattgcagacggccacggccacgtcccggcgtttcccgggggaggctctcggccttcctcccgtgaggtgcgtcggctcctcccgcacgcggctgaccgagggggcggccacgacttgcgggggcggtctgcaaaccgcggcaaaggtacccaaatagttcgattcggcagacagtaaaataaatactcttgtcgtgtcgtttatggagacgaaggggggcggcgtcccgctctttgcgatcgactctccgcatcgcccccgtgggccgtagcatccgcaggcgcagccggcctcggcgaataaggcggagggagtaagaccatcattcgatttatttcttacatttgcacgacggaaaacgaaatagacacctattctctatcctttcgtcctgcggtatcgagtatcctgacagtctcattccgagatgccgtatccccctatcaacacttatcatatttctaaaaagctcatcatcatttatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aacttgcttattattagtttcttgttatttgatgaccactcatacccgatacatccctcaagtcctccgaaaaaaaacgtctggacgttcggaggaaaacgtctagacgttccgaataaaacgtctagacgtttcgagcaaaaggtctagacgtttcgggcaaaacgtctagacgttttttcgaggggatattgcagacggccacggccacgtcccggcgtttcccgggggaggctctcggccttcctcccgtgaggtgcgtcggctcctcccgcacgcggctgaccgagggggcggccacgacttgcgggggcggtctgcaaaccgcggcaaaggtacccaaatagttcgattcggcagacagtaaaataaatactcttgtcgtgtcgtttatggagacgaaggggggcggcgtcccgctctttgcgatcgactctccgcatcgcccccgtgggccgtagcatccgcaggcgcagccggcctcggcgaataaggcggagggagtaagaccatcattcgatttatttcttacatttgcacgacggaaaacgaaatagacacctattctctatcctttcgtcctgcggtatcgagtatcctgacagtctcattccgagatgccgtatccccctatcaacacttatcatatttctaaaaagctcatcatcatttatt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -9.5 Tail_Score: -5.75342 Start: 78 End: 109 Full_Region: acgttttattcggaa cgtctagacgttt tcctcc gaacgtccagacg ttttttttcggagga .............................................................................cgtctagacgttttcctccgaacgtccagacg...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 89 HP_score: -13.2 Tail_Score: -3.53091 Start: 100 End: 131 Full_Region: ACGTTCGGAGGAAAA CGTCTAGACGTT CCGAATAA AACGTCTAGACG TTTCGAGCAAAAGGT ...................................................................................................CGTCTAGACGTTCCGAATAAAACGTCTAGACG................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 100 HP_score: -14.6 Tail_Score: -4.25283 Start: 100 End: 131 Full_Region: accttttgctcgaaa cgtctagacgtttt attc ggaacgtctagacg ttttcctccgaacgt ...................................................................................................cgtctagacgttttattcggaacgtctagacg................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 100 HP_score: -11.1 Tail_Score: -5.00884 Start: 122 End: 175 Full_Region: ACGTTCCGAATAAAA CGTCTAGACGTTTCGAGCAAAAGGT CTAG ACGTTTCGGGCAAAACGTCTAGACG TTTTTTCGAGGGGAT .........................................................................................................................CGTCTAGACGTTTCGAGCAAAAGGTCTAGACGTTTCGGGCAAAACGTCTAGACG.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -15.5 Tail_Score: -4.63486 Start: 122 End: 175 Full_Region: atcccctcgaaaaaa cgtctagacgttttgcccgaaacgt ctag accttttgctcgaaacgtctagacg ttttattcggaacgt .........................................................................................................................cgtctagacgttttgcccgaaacgtctagaccttttgctcgaaacgtctagacg.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 89 HP_score: -13.8 Tail_Score: -3.60048 Start: 145 End: 174 Full_Region: CGTTTCGAGCAAAAG GTCTAGACGTTT CGGGCA AAACGTCTAGAC GTTTTTTCGAGGGGA ................................................................................................................................................GTCTAGACGTTTCGGGCAAAACGTCTAGAC..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 52 HP_score: -5.2 Tail_Score: -3.16683 Start: 200 End: 243 Full_Region: GGGGATATTGCAGAC GGCCACGGCCACGTCCCGG CGTTTC CCGGGGGAGGCTCTCGGCC TTCCTCCCGTGAGGT .......................................................................................................................................................................................................GGCCACGGCCACGTCCCGGCGTTTCCCGGGGGAGGCTCTCGGCC................................................................................................................................................................................................................................................................................................................................................................................................................................................ Find igs database================================================ Query_seq: PG0330:PG0332|PG0330:PG0332:DNA-binding protein, histone-like family:transcription termination factor Rho:<-->:365084..365758 675 aacttgcttattattagtttcttgttatttgatgaccactcatacccgatacatccctcaagtcctccgaaaaaaaacgtctggacgttcggaggaaaacgtctagacgttccgaataaaacgtctagacgtttcgagcaaaaggtctagacgtttcgggcaaaacgtctagacgttttttcgaggggatattgcagacggccacggccacgtcccggcgtttcccgggggaggctctcggccttcctcccgtgaggtgcgtcggctcctcccgcacgcggctgaccgagggggcggccacgacttgcgggggcggtctgcaaaccgcggcaaaggtacccaaatagttcgattcggcagacagtaaaataaatactcttgtcgtgtcgtttatggagacgaaggggggcggcgtcccgctctttgcgatcgactctccgcatcgcccccgtgggccgtagcatccgcaggcgcagccggcctcggcgaataaggcggagggagtaagaccatcattcgatttatttcttacatttgcacgacggaaaacgaaatagacacctattctctatcctttcgtcctgcggtatcgagtatcctgacagtctcattccgagatgccgtatccccctatcaacacttatcatatttctaaaaagctcatcatcatttatt Intra-Species Hit: Count: 2 Min: 1 Max: 675 Len: 675 Subject: NC_002950_PG0330_PG0332|DNA-binding protein, histone-like family:transcription termination factor Rho|NEGATIVE:POSITIVE|[365084,365758]|675 HSP 1 e-value: 0.0 bit: 1291.0 Len: 675 Query Start:1 Query End:675 Subject Strand: POSITIVE Subject Start: 1 Subject End: 675 aacttgcttattattagtttcttgttatttgatgaccactcatacccgatacatccctcaagtcctccgnnnnnnnncgtctggacgttcggaggaaaacgtctagacgttccgaataaaacgtctagacgtttcgagcaaaaggtctagacgtttcgggcaaaacgtctagacgttttttcgaggggatattgcagacggccacggccacgtcccggcgtttcccgggggaggctctcggccttcctcccgtgaggtgcgtcggctcctcccgcacgcggctgaccgagggggcggccacgacttgcgggggcggtctgcaaaccgcggcaaaggtacccaaatagttcgattcggcagacagtaaaataaatactcttgtcgtgtcgtttatggagacgaaggggggcggcgtcccgctctttgcgatcgactctccgcatcgcccccgtgggccgtagcatccgcaggcgcagccggcctcggcgaataaggcggagggagtaagaccatcattcgatttatttcttacatttgcacgacggaaaacgaaatagacacctattctctatcctttcgtcctgcggtatcgagtatcctgacagtctcattccgagatgccgtatccccctatcaacacttatcatatttctaaaaagctcatcatcatttatt aacttgcttattattagtttcttgttatttgatgaccactcatacccgatacatccctcaagtcctccgaaaaaaaacgtctggacgttcggaggaaaacgtctagacgttccgaataaaacgtctagacgtttcgagcaaaaggtctagacgtttcgggcaaaacgtctagacgttttttcgaggggatattgcagacggccacggccacgtcccggcgtttcccgggggaggctctcggccttcctcccgtgaggtgcgtcggctcctcccgcacgcggctgaccgagggggcggccacgacttgcgggggcggtctgcaaaccgcggcaaaggtacccaaatagttcgattcggcagacagtaaaataaatactcttgtcgtgtcgtttatggagacgaaggggggcggcgtcccgctctttgcgatcgactctccgcatcgcccccgtgggccgtagcatccgcaggcgcagccggcctcggcgaataaggcggagggagtaagaccatcattcgatttatttcttacatttgcacgacggaaaacgaaatagacacctattctctatcctttcgtcctgcggtatcgagtatcctgacagtctcattccgagatgccgtatccccctatcaacacttatcatatttctaaaaagctcatcatcatttatt Subject: NC_002950_PG0144_PG0145|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[167759,167970]|212 HSP 1 e-value: 7.0E-12 bit: 73.8 Len: 85 Query Start:96 Query End:180 Subject Strand: NEGATIVE Subject Start: 56 Subject End: 140 ...............................................................................................aaaacgtctagacgttccgaataaaacgtctagacgtttcgagcaaaaggtctagacgtttcgggcaaaacgtctagacgttttt............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ...............................................................................................aaaacgtctagacgttccgacaaaaacgtctagacgttttgactgaaacgtctagacgtatcgaagcaaacgtctagacgttttt............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-7 bit: 60.0 Len: 102 Query Start:78 Query End:179 Subject Strand: NEGATIVE Subject Start: 35 Subject End: 136 .............................................................................cgtctggacgttcggaggaaaacgtctagacgttccgaataaaacgtctagacgtttcgagcaaaaggtctagacgtttcgggcaaaacgtctagacgtttt................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ .............................................................................cgtctagacgttccgacaaaaacgtctagacgttttgactgaaacgtctagacgtatcgaagcaaacgtctagacgtttttcgcgaaaggtctagacgtttt................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ HSP 3 e-value: 4.0E-7 bit: 58.0 Len: 85 Query Start:96 Query End:180 Subject Strand: POSITIVE Subject Start: 35 Subject End: 119 ...............................................................................................aaaacgtctagacgttccgaataaaacgtctagacgtttcgagcaaaaggtctagacgtttcgggcaaaacgtctagacgttttt............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ...............................................................................................aaaacgtctagacctttcgcgaaaaacgtctagacgtttgcttcgatacgtctagacgtttcagtcaaaacgtctagacgttttt............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 4 e-value: 7.0E-6 bit: 54.0 Len: 63 Query Start:121 Query End:183 Subject Strand: POSITIVE Subject Start: 82 Subject End: 144 ........................................................................................................................acgtctagacgtttcgagcaaaaggtctagacgtttcgggcaaaacgtctagacgttttttcg............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ ........................................................................................................................acgtctagacgtttcagtcaaaacgtctagacgtttttgtcggaacgtctagacgttttttcg............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACUUGCUUAUUAUUAGUUUCUUGUUAUUUGAUGACCACUCAUACCCGAUACAUCCCUCAAGUCCUCCGAAAAAAAACGUCUGGACGUUCGGAGGAAAACGUCUAGACGUUCCGAAUAAAACGUCUAGACGUUUCGAGCAAAAGGUCUAGACGUUUCGGGCAAAACGUCUAGACGUUUUUUCGAGGGGAUAUUGCAGACGGCCACGGCCACGUCCCGGCGUUUCCCGGGGGAGGCUCUCGGCCUUCCUCCCGUGAGGUGCGUCGGCUCCUCCCGCACGCGGCUGACCGAGGGGGCGGCCACGACUUGCGGGGGCGGUCUGCAAACCGCGGCAAAGGUACCCAAAUAGUUCGAUUCGGCAGACAGUAAAAUAAAUACUCUUGUCGUGUCGUUUAUGGAGACGAAGGGGGGCGGCGUCCCGCUCUUUGCGAUCGACUCUCCGCAUCGCCCCCGUGGGCCGUAGCAUCCGCAGGCGCAGCCGGCCUCGGCGAAUAAGGCGGAGGGAGUAAGACCAUCAUUCGAUUUAUUUCUUACAUUUGCACGACGGAAAACGAAAUAGACACCUAUUCUCUAUCCUUUCGUCCUGCGGUAUCGAGUAUCCUGACAGUCUCAUUCCGAGAUGCCGUAUCCCCCUAUCAACACUUAUCAUAUUUCUAAAAAGCUCAUCAUCAUUUAUU .....((((.(((...((((((..(((((((...(((.......((((.....((((((...((((((((......((((....))))))))))))((((((((((((((((((((....((((((((((.(((......))).))))))))))))))))....))))))))))))))....))))))...((((((((.(((.(((..((((....))))...))).(((((((.....))))))).((((..((...(((.((((.(((((.....(((....))).))))).))))))).))..))))))).))))))))..)).))....)))...)))))))..((((.((((((..((((.......)))).)))))).)))).....))))))..((((((((((((((.(((.....))).......((((((.(((((.(((((.(((...((....)).))).))..)))....))))).....))))))(((((.((((..(((((((((.............(((((.(((((((...(((((((....))))).)).....))))))).))))).)))))).....)))..)))).)))))..))))))))..))))))....................))).))))............... (-260.04) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAAAUGAUGAUGAGCUUUUUAGAAAUAUGAUAAGUGUUGAUAGGGGGAUACGGCAUCUCGGAAUGAGACUGUCAGGAUACUCGAUACCGCAGGACGAAAGGAUAGAGAAUAGGUGUCUAUUUCGUUUUCCGUCGUGCAAAUGUAAGAAAUAAAUCGAAUGAUGGUCUUACUCCCUCCGCCUUAUUCGCCGAGGCCGGCUGCGCCUGCGGAUGCUACGGCCCACGGGGGCGAUGCGGAGAGUCGAUCGCAAAGAGCGGGACGCCGCCCCCCUUCGUCUCCAUAAACGACACGACAAGAGUAUUUAUUUUACUGUCUGCCGAAUCGAACUAUUUGGGUACCUUUGCCGCGGUUUGCAGACCGCCCCCGCAAGUCGUGGCCGCCCCCUCGGUCAGCCGCGUGCGGGAGGAGCCGACGCACCUCACGGGAGGAAGGCCGAGAGCCUCCCCCGGGAAACGCCGGGACGUGGCCGUGGCCGUCUGCAAUAUCCCCUCGAAAAAACGUCUAGACGUUUUGCCCGAAACGUCUAGACCUUUUGCUCGAAACGUCUAGACGUUUUAUUCGGAACGUCUAGACGUUUUCCUCCGAACGUCCAGACGUUUUUUUUCGGAGGACUUGAGGGAUGUAUCGGGUAUGAGUGGUCAUCAAAUAACAAGAAACUAAUAAUAAGCAAGUU ...............((((.((((.....((((..(((..(((((((((...((((.....(((.(((((((((((......(((((...(((.((((.((((...((((.(((....))))))).)))).)))).))).......(....)..))))).))))))))))).)))((((((.......((((....))))..(((((.((...((....))...)).)))))..)))))).(((..((((.....))))))))))).))))))..)))..)))...((.((.(((((((((....)))))..)))))).)).))))...(((((((..(((..((((.((((((((((((.(((((((((...((((((.(((.....)))..))))))))))))..(.(((.(((..((...((((.(((.(((.....))))))))))(....)...))..)))))))..))).))))))))....(((((((..((((((((((((((((((...(((((((((((((.((((....)))).)))))))))))))...))))))))))))))))))((((((((((((....))))......)))))))).)))))))....)))))))).....)))..)))))))........))))....))))..... (-265.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 952 110 61 201 152 NC_002950:PG0330|5end_DNA-binding_protein,_histone-like_family_364993..365223_NEGATIVE 610 130 81 198 150 NC_002950:PG0145|5end_hypothetical_protein_167831..168061_POSITIVE 575 487 443 76 21 NC_002950:PG0691|5end_NifU-related_protein_747469..747699_NEGATIVE 572 170 121 200 151 NC_002950:PG0144|5end_hypothetical_protein_167668..167898_NEGATIVE 562 485 442 57 3 NC_002950:PG1992|5end_glucose-inhibited_division_protein_A_2081410..2081640_POSITIVE 534 257 212 59 6 NC_002950:PG2225|5end_hypothetical_protein_2339440..2339670_POSITIVE 529 317 272 68 14 NC_002950:PG2111|5end_thiS_protein_2221482..2221712_NEGATIVE 512 330 283 224 157 NC_002950:PG0152|5end_carboxynorspermidine_decarboxylase_175657..175887_NEGATIVE 511 249 201 123 61 NC_002950:PG0158|5end_competence_protein_F-related_protein_180175..180405_POSITIVE 508 490 448 52 7 NC_002950:PG1772|5end_endonuclease_III_1859079..1859309_POSITIVE 507 458 412 223 171 NC_002950:PG1475|5end_conjugative_transposon_protein_TraN_1551835..1552065_NEGATIVE 507 479 438 53 1 NC_002950:PG1043|5end_ferrous_iron_transport_protein_B_1112852..1113082_NEGATIVE 506 480 431 124 62 NC_002950:PG0362|5end_hypothetical_protein_395301..395531_POSITIVE 497 318 274 193 146 NC_002950:PG2189|5end_aspartate_kinase_2297151..2297381_NEGATIVE 497 270 221 189 136 NC_002950:PG0259|5end_hypothetical_protein_292278..292508_POSITIVE 496 320 276 203 148 NC_002950:PG1102|5end_hypothetical_protein_1173637..1173867_NEGATIVE 494 259 212 117 62 NC_002950:PG1388|5end_hypothetical_protein_1470889..1471119_NEGATIVE 488 279 233 225 168 NC_002950:PG0976|5end_phosphoribosylaminoimidazole-succinocarboxamide_synthase_1038326..1038556_POSITIVE 484 300 251 120 70 NC_002950:PG0447|5end_hypothetical_protein_487278..487508_POSITIVE 483 327 285 74 29 NC_002950:PG0171|5end_5'-nucleotidase_family_protein_191724..191954_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 559 250 202 76 17 NC_002950:PG0143|3end_hydrolase,_carbon-nitrogen_family_165747..165897_NEGATIVE 511 249 201 139 77 NC_002950:PG0157|3end_regulatory_protein_RecX_180271..180421_POSITIVE 503 350 306 58 6 NC_002950:PG0361|3end_hypothetical_protein_395481..395631_POSITIVE 494 259 212 136 81 NC_002950:PG1389|3end_DNA-binding_protein,_histone-like_family_1470870..1471020_NEGATIVE 484 300 251 148 98 NC_002950:PG0446|3end_thiF_protein_487386..487536_POSITIVE 473 480 435 68 15 NC_002950:PG1007|3end_transcriptional_regulator,_GntR_family_1066518..1066668_NEGATIVE 466 250 202 115 42 NC_002950:PG1416|3end_enoyl-(acyl-carrier-protein)_reductase_II_1501078..1501228_POSITIVE 461 250 201 62 12 NC_002950:PG0803|3end_glucosamine-6-phosphate_deaminase_859727..859877_NEGATIVE 461 337 294 135 88 NC_002950:PG0746|3end_sensor_histidine_kinase_792536..792686_NEGATIVE 459 318 274 150 107 NC_002950:PG0860|3end_transcriptional_regulator,_putative_919273..919423_POSITIVE 458 489 441 87 41 NC_002950:PG2026|3end_phosphoglycerate_mutase_family_protein_2125545..2125695_POSITIVE 457 330 281 107 62 NC_002950:PG1972|3end_hemagglutinin_protein_HagB_2061203..2061353_POSITIVE 455 429 381 115 60 NC_002950:PG1494|3end_hypothetical_protein_1571234..1571384_POSITIVE 453 330 281 75 23 NC_002950:PG2063|3end_hypothetical_protein_2160390..2160540_NEGATIVE 451 310 261 102 46 NC_002950:PG1741|3end_aspartate_ammonia-lyase_1825856..1826006_NEGATIVE 450 310 261 89 25 NC_002950:PG1977|3end_hypothetical_protein_2064687..2064837_NEGATIVE 449 500 453 151 94 NC_002950:PG2040|3end_DNA-binding_protein,_histone-like_family_2138398..2138548_NEGATIVE 447 260 215 49 2 NC_002950:PG0127|3end_ferrochelatase_149667..149817_POSITIVE 446 327 281 60 16 NC_002950:PG0381|3end_cell_volume_regulation_protein_CvrA_411185..411335_NEGATIVE 444 229 183 129 80 NC_002950:PG0164|3end_hypothetical_protein_187081..187231_NEGATIVE