Origin IGS: ggacaaaggaatcggacgatcatagcaatgggaggaaaccgactgatacccgattgacctgaaaaggtatatgctggcaaaccggagaattcttctatttttgcgcatgtttttgcgcgaagtcggttagaaatctttccggccgaggcaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttgcctcggccggaaagatttctaaccgacttcgcgcaaaaacatgcgcaaaaatagaagaattctccggtttgccagcatataccttttcaggtcaatcgggtatcagtcggtttcctcccattgctatgatcgtccgattcctttgtcc Mask Tandem Repeat Region ================================================ ggacaaaggaatcggacgatcatagcaatgggaggaaaccgactgatacccgattgacctgaaaaggtatatgctggcaaaccggagaattcttctatttttgcgcatgtttttgcgcgaagtcggttagaaatctttccggccgaggcaaa Find is-nt database================================================ Query_seq: PG0285:PG0286|PG0285:PG0286:hypothetical protein:hypothetical protein:->->:320105..320256 152 ggacaaaggaatcggacgatcatagcaatgggaggaaaccgactgatacccgattgacctgaaaaggtatatgctggcaaaccggagaattcttctatttttgcgcatgtttttgcgcgaagtcggttagaaatctttccggccgaggcaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0285:PG0286|PG0285:PG0286:hypothetical protein:hypothetical protein:->->:320105..320256 152 ggacaaaggaatcggacgatcatagcaatgggaggaaaccgactgatacccgattgacctgaaaaggtatatgctggcaaaccggagaattcttctatttttgcgcatgtttttgcgcgaagtcggttagaaatctttccggccgaggcaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0285:PG0286|PG0285:PG0286:hypothetical protein:hypothetical protein:->->:320105..320256 152 ggacaaaggaatcggacgatcatagcaatgggaggaaaccgactgatacccgattgacctgaaaaggtatatgctggcaaaccggagaattcttctatttttgcgcatgtttttgcgcgaagtcggttagaaatctttccggccgaggcaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggacaaaggaatcggacgatcatagcaatgggaggaaaccgactgatacccgattgacctgaaaaggtatatgctggcaaaccggagaattcttctatttttgcgcatgtttttgcgcgaagtcggttagaaatctttccggccgaggcaaa Predict ORF larger than 30AA ================================================ Protein_Len: 44 Strand: - Start: 3 End: 134 .. C L F R V I M A I P P F R S I G S Q G S F T Y A P L G S F E E I K A C T K A R L R N S M .................. Protein_Len: 36 Strand: - Start: 1 End: 108 S L P I P R D Y C H S S V S Q Y G I S R F L Y I S A F R L I R R N K R M ............................................ ggacaaaggaatcggacgatcatagcaatgggaggaaaccgactgatacccgattgacctgaaaaggtatatgctggcaaaccggagaattcttctatttttgcgcatgtttttgcgcgaagtcggttagaaatctttccggccgaggcaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggacaaaggaatcggacgatcatagcaatgggaggaaaccgactgatacccgattgacctgaaaaggtatatgctggcaaaccggagaattcttctatttttgcgcatgtttttgcgcgaagtcggttagaaatctttccggccgaggcaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0285:PG0286|PG0285:PG0286:hypothetical protein:hypothetical protein:->->:320105..320256 152 ggacaaaggaatcggacgatcatagcaatgggaggaaaccgactgatacccgattgacctgaaaaggtatatgctggcaaaccggagaattcttctatttttgcgcatgtttttgcgcgaagtcggttagaaatctttccggccgaggcaaa Intra-Species Hit: Count: 1 Min: 1 Max: 152 Len: 152 Subject: NC_002950_PG0285_PG0286|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[320105,320256]|152 HSP 1 e-value: 3.0E-81 bit: 301.0 Len: 152 Query Start:1 Query End:152 Subject Strand: POSITIVE Subject Start: 1 Subject End: 152 ggacaaaggaatcggacgatcatagcaatgggaggaaaccgactgatacccgattgacctgaaaaggtatatgctggcaaaccggagaattcttctatttttgcgcatgtttttgcgcgaagtcggttagaaatctttccggccgaggcaaa ggacaaaggaatcggacgatcatagcaatgggaggaaaccgactgatacccgattgacctgaaaaggtatatgctggcaaaccggagaattcttctatttttgcgcatgtttttgcgcgaagtcggttagaaatctttccggccgaggcaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGACAAAGGAAUCGGACGAUCAUAGCAAUGGGAGGAAACCGACUGAUACCCGAUUGACCUGAAAAGGUAUAUGCUGGCAAACCGGAGAAUUCUUCUAUUUUUGCGCAUGUUUUUGCGCGAAGUCGGUUAGAAAUCUUUCCGGCCGAGGCAAA ........(..((((.......(((((..(((((........))....))).....((((....))))...))))).....(((((((.((((.((..(((((((((......)))))))))..))..))))...)))))))))))..)... (-45.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUGCCUCGGCCGGAAAGAUUUCUAACCGACUUCGCGCAAAAACAUGCGCAAAAAUAGAAGAAUUCUCCGGUUUGCCAGCAUAUACCUUUUCAGGUCAAUCGGGUAUCAGUCGGUUUCCUCCCAUUGCUAUGAUCGUCCGAUUCCUUUGUCC ......((((.(((..........((((((((..(((((......)))))................(((((((.(((................))).)))))))....))))))))......(((....))).))).))))........... (-35.89) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 425 149 103 154 118 NC_002950:PG1476|5end_conjugative_transposon_protein_TraM_1553219..1553449_NEGATIVE 409 150 102 193 153 NC_002950:PG1556|5end_hypothetical_protein_1636595..1636825_POSITIVE 384 150 101 83 18 NC_002950:PG0524|5end_hypothetical_protein_568599..568829_POSITIVE 357 147 101 156 103 NC_002950:PG1617|5end_hypothetical_protein_1697755..1697985_NEGATIVE 354 77 32 222 178 NC_002950:PG1790|5end_hypothetical_protein_1880954..1881184_NEGATIVE 342 150 108 206 159 NC_002950:PG0751|5end_porT_protein_797578..797808_NEGATIVE 338 149 101 88 37 NC_002950:PG0606|5end_hypothetical_protein_662558..662788_NEGATIVE 338 149 101 69 17 NC_002950:PG0217|5end_hypothetical_protein_252490..252720_POSITIVE 336 148 101 59 18 NC_002950:PG0927|5end_conserved_hypothetical_protein_TIGR00150_985050..985280_NEGATIVE 335 150 101 80 15 NC_002950:PG2119|5end_oxidoreductase,_Gfo/Idh/MocA_family_2226547..2226777_POSITIVE 329 150 103 193 132 NC_002950:PG1057|5end_hypothetical_protein_1123419..1123649_POSITIVE 328 145 101 64 19 NC_002950:PG1486|5end_conjugative_transposon_protein_TraA_1561166..1561396_NEGATIVE 325 149 103 63 1 NC_002950:PG0269|5end_exodeoxyribonuclease_III_302028..302258_POSITIVE 322 148 104 230 188 NC_002950:PG0661|5end_hypothetical_protein_709853..710083_NEGATIVE 321 150 114 195 151 NC_002950:PG1972|5end_hemagglutinin_protein_HagB_2060002..2060232_POSITIVE 320 146 108 37 1 NC_002950:PG1917|5end_methionine_aminopeptidase,_type_I_2014490..2014720_NEGATIVE 320 107 61 210 171 NC_002950:PG1739|5end_hypothetical_protein_1825709..1825939_NEGATIVE 320 146 103 80 29 NC_002950:PG1214|5end_hypothetical_protein_1293032..1293262_POSITIVE 320 59 11 58 4 NC_002950:PG0213|5end_precorrin-3_methylase/precorrin-8X_methylmutase_250464..250694_NEGATIVE 319 150 105 56 5 NC_002950:PG2209|5end_hypothetical_protein_2320928..2321158_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 350 119 74 146 102 NC_002950:PG0377|3end_30S_ribosomal_protein_S2_407845..407995_POSITIVE 336 50 3 57 11 NC_002950:PG1472|3end_hypothetical_protein_1549760..1549910_POSITIVE 336 148 101 47 6 NC_002950:PG0928|3end_response_regulator_985062..985212_NEGATIVE 325 145 101 149 104 NC_002950:PG0086|3end_ATP-dependent_RNA_helicase,_DEAD/DEAH_box_family_100545..100695_NEGATIVE 324 118 76 88 32 NC_002950:PG0380|3end_excinuclease_ABC_subunit_B_411218..411368_POSITIVE 320 146 108 37 1 NC_002950:PG1918|3end_preprotein_translocase_SecY_2014490..2014640_NEGATIVE 320 107 61 63 24 NC_002950:PG1741|3end_aspartate_ammonia-lyase_1825856..1826006_NEGATIVE 320 146 103 131 80 NC_002950:PG1213|3end_ribonuclease_H_1293163..1293313_POSITIVE 319 148 103 121 63 NC_002950:PG0628|3end_ABC_transporter,_ATP-binding_protein_681684..681834_NEGATIVE 317 50 3 95 56 NC_002950:PG1653|3end_hypothetical_protein_1736458..1736608_POSITIVE 316 150 105 48 15 NC_002950:PG0160|3end_hypothetical_protein_183183..183333_NEGATIVE 315 150 104 82 35 NC_002950:PG1948|3end_lipoprotein,_putative_2037940..2038090_POSITIVE 314 150 101 112 44 NC_002950:PG1392|3end_rod_shape-determining_protein_RodA,_putative_1472959..1473109_NEGATIVE 314 129 82 146 96 NC_002950:PG0390|3end_50S_ribosomal_protein_L11_423339..423489_POSITIVE 311 78 32 147 97 NC_002950:PG1608|3end_methylmalonyl-CoA_decarboxylase,_beta_subunit_1688685..1688835_NEGATIVE 307 148 102 127 76 NC_002950:PG1012|3end_tRNA-i(6)A37_modification_enzyme_MiaB_1070089..1070239_NEGATIVE 306 149 115 103 55 NC_002950:PG0766|3end_polyribonucleotide_nucleotidyltransferase_816174..816324_NEGATIVE 306 147 102 150 102 NC_002950:PG0162|3end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_184505..184655_NEGATIVE 298 148 104 145 110 NC_002950:PG2001|3end_signal_peptidase_I_2089458..2089608_NEGATIVE 298 86 42 52 11 NC_002950:PG0583|3end_cell_division_protein_FtsA_641944..642094_POSITIVE