Origin IGS: ctttgcgatatgctttttctatccttttggaagggccgaatcaaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagtttggtttagcccccttttgaggtcgattcgggcatctctcaagactggtcattaaaaccctgtatttcagttga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tcaactgaaatacagggttttaatgaccagtcttgagagatgcccgaatcgacctcaaaagggggctaaaccaaactatatagtttgaccaaaccaaagtatatagtttggttcaaccaaagtatatagttcggtcagaccaaactatatagtttagtcagaccaaagtatatagtttgattcggcccttccaaaaggatagaaaaagcatatcgcaaag Mask Tandem Repeat Region ================================================ ctttgcgatatgctttttctatccttttggaagggccgaatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttagcccccttttgaggtcgattcgggcatctctcaagactggtcattaaaaccctgtatttcagttga Find is-nt database================================================ Query_seq: PG0281:PG0282|PG0281:PG0282:ABC transporter, permease protein, putative:ABC transporter, ATP-binding protein:<-<-:316114..316333 220 ctttgcgatatgctttttctatccttttggaagggccgaatcaaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagtttggtttagcccccttttgaggtcgattcgggcatctctcaagactggtcattaaaaccctgtatttcagttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0281:PG0282|PG0281:PG0282:ABC transporter, permease protein, putative:ABC transporter, ATP-binding protein:<-<-:316114..316333 220 ctttgcgatatgctttttctatccttttggaagggccgaatcaaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagtttggtttagcccccttttgaggtcgattcgggcatctctcaagactggtcattaaaaccctgtatttcagttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0281:PG0282|PG0281:PG0282:ABC transporter, permease protein, putative:ABC transporter, ATP-binding protein:<-<-:316114..316333 220 ctttgcgatatgctttttctatccttttggaagggccgaatcaaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagtttggtttagcccccttttgaggtcgattcgggcatctctcaagactggtcattaaaaccctgtatttcagttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctttgcgatatgctttttctatccttttggaagggccgaatcaaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagtttggtttagcccccttttgaggtcgattcgggcatctctcaagactggtcattaaaaccctgtatttcagttga Predict ORF larger than 30AA ================================================ ctttgcgatatgctttttctatccttttggaagggccgaatcaaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagtttggtttagcccccttttgaggtcgattcgggcatctctcaagactggtcattaaaaccctgtatttcagttga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctttgcgatatgctttttctatccttttggaagggccgaatcaaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagtttggtttagcccccttttgaggtcgattcgggcatctctcaagactggtcattaaaaccctgtatttcagttga Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 72 HP_score: -6.9 Tail_Score: -3.90856 Start: 46 End: 99 Full_Region: tttggttcaaccaaa gtatatagttcggtcagaccaaact atat agtttagtcagaccaaagtatatag tttgattcggccctt .............................................gtatatagttcggtcagaccaaactatatagtttagtcagaccaaagtatatag......................................................................................................................... TransTerm Strand: + Conf: 86 HP_score: -11.3 Tail_Score: -3.90112 Start: 46 End: 99 Full_Region: AAGGGCCGAATCAAA CTATATACTTTGGTCTGACTAAACT ATAT AGTTTGGTCTGACCGAACTATATAC TTTGGTTGAACCAAA .............................................CTATATACTTTGGTCTGACTAAACTATATAGTTTGGTCTGACCGAACTATATAC......................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -14.8 Tail_Score: -2.93853 Start: 47 End: 98 Full_Region: AGGGCCGAATCAAAC TATATACTTTGGTCTGACTAAACT ATAT AGTTTGGTCTGACCGAACTATATA CTTTGGTTGAACCAA ..............................................TATATACTTTGGTCTGACTAAACTATATAGTTTGGTCTGACCGAACTATATA.......................................................................................................................... TransTerm Strand: - Conf: 77 HP_score: -10.4 Tail_Score: -2.94032 Start: 47 End: 98 Full_Region: ttggttcaaccaaag tatatagttcggtcagaccaaact atat agtttagtcagaccaaagtatata gtttgattcggccct ..............................................tatatagttcggtcagaccaaactatatagtttagtcagaccaaagtatata.......................................................................................................................... TransTerm Strand: + Conf: 91 HP_score: -13.0 Tail_Score: -3.02166 Start: 49 End: 96 Full_Region: GGCCGAATCAAACTA TATACTTTGGTCTGACTAAACT ATAT AGTTTGGTCTGACCGAACTATA TACTTTGGTTGAACC ................................................TATACTTTGGTCTGACTAAACTATATAGTTTGGTCTGACCGAACTATA............................................................................................................................ TransTerm Strand: + Conf: 75 HP_score: -7.7 Tail_Score: -4.10552 Start: 69 End: 122 Full_Region: TTTGGTCTGACTAAA CTATATAGTTTGGTCTGACCGAACT ATAT ACTTTGGTTGAACCAAACTATATAC TTTGGTTTGGTCAAA ....................................................................CTATATAGTTTGGTCTGACCGAACTATATACTTTGGTTGAACCAAACTATATAC.................................................................................................. TransTerm Strand: - Conf: 70 HP_score: -7.0 Tail_Score: -3.72576 Start: 69 End: 99 Full_Region: tttggttcaaccaaa gtatatagttcggt cag accaaactatatag tttagtcagaccaaa ....................................................................gtatatagttcggtcagaccaaactatatag......................................................................................................................... TransTerm Strand: + Conf: 80 HP_score: -11.2 Tail_Score: -3.05849 Start: 70 End: 121 Full_Region: TTGGTCTGACTAAAC TATATAGTTTGGTCTGACCGAACT ATAT ACTTTGGTTGAACCAAACTATATA CTTTGGTTTGGTCAA .....................................................................TATATAGTTTGGTCTGACCGAACTATATACTTTGGTTGAACCAAACTATATA................................................................................................... TransTerm Strand: - Conf: 76 HP_score: -10.5 Tail_Score: -2.83403 Start: 70 End: 98 Full_Region: ttggttcaaccaaag tatatagttcggt cag accaaactatata gtttagtcagaccaa .....................................................................tatatagttcggtcagaccaaactatata.......................................................................................................................... TransTerm Strand: + Conf: 74 HP_score: -9.4 Tail_Score: -3.08002 Start: 72 End: 119 Full_Region: GGTCTGACTAAACTA TATAGTTTGGTCTGACCGAACT ATAT ACTTTGGTTGAACCAAACTATA TACTTTGGTTTGGTC .......................................................................TATAGTTTGGTCTGACCGAACTATATACTTTGGTTGAACCAAACTATA..................................................................................................... TransTerm Strand: + Conf: 83 HP_score: -10.7 Tail_Score: -3.74251 Start: 87 End: 127 Full_Region: TATAGTTTGGTCTGA CCGAACTATATACTTTGGT TGA ACCAAACTATATACTTTGG TTTGGTCAAACTATA ......................................................................................CCGAACTATATACTTTGGTTGAACCAAACTATATACTTTGG............................................................................................. TransTerm Strand: + Conf: 79 HP_score: -8.5 Tail_Score: -4.05788 Start: 92 End: 145 Full_Region: TTTGGTCTGACCGAA CTATATACTTTGGTTGAACCAAACT ATAT ACTTTGGTTTGGTCAAACTATATAG TTTGGTTTAGCCCCC ...........................................................................................CTATATACTTTGGTTGAACCAAACTATATACTTTGGTTTGGTCAAACTATATAG........................................................................... TransTerm Strand: + Conf: 95 HP_score: -12.9 Tail_Score: -3.57309 Start: 110 End: 150 Full_Region: TATACTTTGGTTGAA CCAAACTATATACTTTGGT TTG GTCAAACTATATAGTTTGG TTTAGCCCCCTTTTG .............................................................................................................CCAAACTATATACTTTGGTTTGGTCAAACTATATAGTTTGG...................................................................... TransTerm Strand: - Conf: 59 HP_score: -4.2 Tail_Score: -3.90112 Start: 115 End: 145 Full_Region: gggggctaaaccaaa ctatatagtttg accaaac caaagtatatag tttggttcaaccaaa ..................................................................................................................ctatatagtttgaccaaaccaaagtatatag........................................................................... Find igs database================================================ Query_seq: PG0281:PG0282|PG0281:PG0282:ABC transporter, permease protein, putative:ABC transporter, ATP-binding protein:<-<-:316114..316333 220 ctttgcgatatgctttttctatccttttggaagggccgaatcaaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagtttggtttagcccccttttgaggtcgattcgggcatctctcaagactggtcattaaaaccctgtatttcagttga Intra-Species Hit: Count: 4 Min: 1 Max: 220 Len: 220 Subject: NC_002950_PG0281_PG0282|ABC transporter, permease protein, putative:ABC transporter, ATP-binding protein|NEGATIVE:NEGATIVE|[316114,316333]|220 HSP 1 e-value: 1.0E-121 bit: 436.0 Len: 220 Query Start:1 Query End:220 Subject Strand: POSITIVE Subject Start: 1 Subject End: 220 ctttgcgatatgctttttctatccttttggaagggccgaatcaaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagtttggtttagcccccttttgaggtcgattcgggcatctctcaagactggtcattaaaaccctgtatttcagttga ctttgcgatatgctttttctatccttttggaagggccgaatcaaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagtttggtttagcccccttttgaggtcgattcgggcatctctcaagactggtcattaaaaccctgtatttcagttga Subject: NC_002950_PG0555_PG0556|DNA-binding protein, histone-like family:hypothetical protein|NEGATIVE:POSITIVE|[612325,613417]|1093 HSP 1 e-value: 2.0E-25 bit: 117.0 Len: 111 Query Start:39 Query End:149 Subject Strand: NEGATIVE Subject Start: 226 Subject End: 336 ......................................aatcaaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagtttg....................................................................... ......................................aatcaaactatatactttggcaaaactaaactatatagtttggtgagactaaactatatactttggttcaaccaaactatatactttggtgagaccaaactatatagtttg....................................................................... HSP 2 e-value: 9.0E-15 bit: 81.8 Len: 97 Query Start:57 Query End:153 Subject Strand: POSITIVE Subject Start: 218 Subject End: 314 ........................................................ggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagtttggttt................................................................... ........................................................ggtctgatcaaactatatagtttggtctcaccaaagtatatagtttggttgaaccaaagtatatagtttagtctcaccaaactatatagtttagttt................................................................... HSP 3 e-value: 1.0E-10 bit: 67.9 Len: 110 Query Start:40 Query End:149 Subject Strand: POSITIVE Subject Start: 224 Subject End: 333 .......................................atcaaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagtttg....................................................................... .......................................atcaaactatatagtttggtctcaccaaagtatatagtttggttgaaccaaagtatatagtttagtctcaccaaactatatagtttagttttgccaaagtatatagtttg....................................................................... HSP 4 e-value: 1.0E-7 bit: 58.0 Len: 49 Query Start:42 Query End:90 Subject Strand: NEGATIVE Subject Start: 216 Subject End: 264 .........................................caaactatatactttggtctgactaaactatatagtttggtctgaccga.................................................................................................................................. .........................................caaactatatactttggtgagaccaaactatatagtttgatcagaccga.................................................................................................................................. Subject: NC_002950_PG0453_PG0456|hypothetical protein:PHP N-terminal domain protein|POSITIVE:POSITIVE|[496731,497854]|1124 HSP 1 e-value: 3.0E-24 bit: 113.0 Len: 121 Query Start:43 Query End:163 Subject Strand: POSITIVE Subject Start: 77 Subject End: 197 ..........................................aaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagtttggtttagcccccttt......................................................... ..........................................aaactatatagtttggttcaactaaactatatactttggtccgactaaactatatactttggtcgaaccaaactatatactttgataaagccaaactatatactttggttttgcccccttt......................................................... HSP 2 e-value: 6.0E-13 bit: 75.8 Len: 110 Query Start:40 Query End:149 Subject Strand: NEGATIVE Subject Start: 53 Subject End: 162 .......................................atcaaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagtttg....................................................................... .......................................atcaaagtatatagtttggttcgaccaaagtatatagtttagtcggaccaaagtatatagtttagttgaaccaaactatatagtttagtttgggccaaatatatggtttg....................................................................... HSP 3 e-value: 3.0E-8 bit: 60.0 Len: 66 Query Start:63 Query End:128 Subject Strand: POSITIVE Subject Start: 74 Subject End: 139 ..............................................................actaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggt............................................................................................ ..............................................................actaaactatatagtttggttcaactaaactatatactttggtccgactaaactatatactttggt............................................................................................ Subject: NC_002950_PG0711_PG0712|hypothetical protein:hypothetical protein|POSITIVE:NEGATIVE|[766342,766873]|532 HSP 1 e-value: 1.0E-23 bit: 111.0 Len: 84 Query Start:43 Query End:126 Subject Strand: POSITIVE Subject Start: 166 Subject End: 249 ..........................................aaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttg.............................................................................................. ..........................................aaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttg.............................................................................................. HSP 2 e-value: 4.0E-14 bit: 79.8 Len: 88 Query Start:43 Query End:130 Subject Strand: POSITIVE Subject Start: 189 Subject End: 276 ..........................................aaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggttt.......................................................................................... ..........................................aaactatatagtttggtcaaaccgaactatatactttggtcgaaccaaactatatactttgatgaaaccaaactatatactttggttt.......................................................................................... HSP 3 e-value: 9.0E-12 bit: 71.9 Len: 92 Query Start:40 Query End:131 Subject Strand: NEGATIVE Subject Start: 160 Subject End: 251 .......................................atcaaactatatactttggtctgactaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttg......................................................................................... .......................................atcaaagtatatagtttggttcgaccaaagtatatagttcggtttgaccaaactatatagtttagttcaaccaaactatatagtttagtttg......................................................................................... HSP 4 e-value: 3.0E-8 bit: 60.0 Len: 66 Query Start:63 Query End:128 Subject Strand: POSITIVE Subject Start: 163 Subject End: 228 ..............................................................actaaactatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggt............................................................................................ ..............................................................actaaactatatagtttggttgaactaaactatatagtttggtcaaaccgaactatatactttggt............................................................................................ HSP 5 e-value: 2.0E-6 bit: 54.0 Len: 79 Query Start:70 Query End:148 Subject Strand: NEGATIVE Subject Start: 189 Subject End: 267 .....................................................................tatatagtttggtctgaccgaactatatactttggttgaaccaaactatatactttggtttggtcaaactatatagttt........................................................................ .....................................................................tatatagtttggtttcatcaaagtatatagtttggttcgaccaaagtatatagttcggtttgaccaaactatatagttt........................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUUUGCGAUAUGCUUUUUCUAUCCUUUUGGAAGGGCCGAAUCAAACUAUAUACUUUGGUCUGACUAAACUAUAUAGUUUGGUCUGACCGAACUAUAUACUUUGGUUGAACCAAACUAUAUACUUUGGUUUGGUCAAACUAUAUAGUUUGGUUUAGCCCCCUUUUGAGGUCGAUUCGGGCAUCUCUCAAGACUGGUCAUUAAAACCCUGUAUUUCAGUUGA ..(((.((.((((((..((...((((..((..((((..((((((((((((((.((((..(.(((((((.(((((((((((((..(((((((........)))))))..))))))))))))).))))))).)..)))).))))))))))))))..))))))....))))..))...)))))).)).)))((((((..................)))))).. (-83.57) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCAACUGAAAUACAGGGUUUUAAUGACCAGUCUUGAGAGAUGCCCGAAUCGACCUCAAAAGGGGGCUAAACCAAACUAUAUAGUUUGACCAAACCAAAGUAUAUAGUUUGGUUCAACCAAAGUAUAUAGUUCGGUCAGACCAAACUAUAUAGUUUAGUCAGACCAAAGUAUAUAGUUUGAUUCGGCCCUUCCAAAAGGAUAGAAAAAGCAUAUCGCAAAG ....(((.....)))((((.....))))....(((.((.((((.....((..(((.....(((((((.((((((((((((((.((((.......)))).))))))))))))))....((((.((((((.((.((((.(((.(((((....))))).))).)))).)).)))))).))))....))))))).....)))...))....)))).)).))).. (-67.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 856 70 21 161 112 NC_002950:PG0281|5end_ABC_transporter,_permease_protein,_putative_316023..316253_NEGATIVE 387 194 156 52 1 NC_002950:PG1656|5end_methylmalonyl-CoA_mutase,_small_subunit_1737459..1737689_POSITIVE 367 186 153 61 18 NC_002950:PG0062|5end_TPR_domain_protein_73688..73918_POSITIVE 334 139 102 142 105 NC_002950:PG0862|5end_type_IIS_restriction_endonuclease,_putative_922740..922970_POSITIVE 329 43 1 182 116 NC_002950:PG1341|5end_hypothetical_protein_1422031..1422261_POSITIVE 328 39 1 196 156 NC_002950:PG0701|5end_cobinamide_kinase/cobinamide_phosphate_guanylyltransferase_756511..756741_POSITIVE 322 210 161 231 178 NC_002950:PG1635|5end_hypothetical_protein_1715861..1716091_NEGATIVE 319 180 131 110 62 NC_002950:PG0769|5end_fibronectin_type_III_domain_protein_824177..824407_NEGATIVE 319 197 153 74 13 NC_002950:PG0377|5end_30S_ribosomal_protein_S2_406920..407150_POSITIVE 314 180 132 180 123 NC_002950:PG0312|5end_hypothetical_protein_347048..347278_NEGATIVE 311 185 145 206 169 NC_002950:PG1805|5end_V-type_ATP_synthase_subunit_D_1899589..1899819_POSITIVE 310 92 55 219 177 NC_002950:PG0841|5end_mobilizable_transposon,_excision_protein,_putative_903674..903904_NEGATIVE 310 187 146 215 158 NC_002950:PG0286|5end_hypothetical_protein_320117..320347_POSITIVE 308 179 139 69 29 NC_002950:PG1760|5end_ABC_transporter,_ATP-binding_protein_1846507..1846737_POSITIVE 306 38 5 188 149 NC_002950:PG1017|5end_pyruvate_phosphate_dikinase_1076138..1076368_POSITIVE 304 195 154 171 129 NC_002950:PG1747|5end_ribose_5-phosphate_isomerase_B,_putative_1832815..1833045_NEGATIVE 302 187 148 90 42 NC_002950:PG2052|5end_dihydrodipicolinate_synthase_2149636..2149866_POSITIVE 302 179 134 133 91 NC_002950:PG1639|5end_hypothetical_protein_1719919..1720149_NEGATIVE 301 195 154 215 158 NC_002950:PG0148|5end_sigma-54-dependent_transcriptional_regulator_170907..171137_NEGATIVE 299 190 142 59 6 NC_002950:PG2032|5end_primosomal_protein_N'_2133381..2133611_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 367 186 153 56 13 NC_002950:PG0061|3end_yngK_protein_73763..73913_POSITIVE 334 139 102 131 94 NC_002950:PG0861|3end_helicase,_SNF2/RAD54_family_922809..922959_POSITIVE 319 180 131 128 80 NC_002950:PG0770|3end_hypothetical_protein_824159..824309_NEGATIVE 310 92 55 83 41 NC_002950:PG0842|3end_mobilizable_transposon,_hypothetical_protein,_putative_903810..903960_NEGATIVE 310 187 146 62 5 NC_002950:PG0285|3end_hypothetical_protein_320044..320194_POSITIVE 308 179 139 79 39 NC_002950:PG1759|3end_adhesion_protein,_putative_1846597..1846747_POSITIVE 304 195 154 84 42 NC_002950:PG1748|3end_transketolase_1832902..1833052_NEGATIVE 302 179 134 117 75 NC_002950:PG1640|3end_DNA-damage-inducible_protein_F_1719935..1720085_NEGATIVE 298 195 157 84 43 NC_002950:PG1906|3end_ISPg1,_transposase_2006572..2006722_POSITIVE 298 195 157 84 43 NC_002950:PG1624|3end_ISPg1,_transposase_1706188..1706338_NEGATIVE 298 195 157 84 43 NC_002950:PG1448|3end_ISPg1,_transposase_1533449..1533599_POSITIVE 298 195 157 84 43 NC_002950:PG1320|3end_ISPg1,_transposase,_internal_deletion_1399467..1399617_POSITIVE 298 195 157 84 43 NC_002950:PG1197|3end_ISPg1,_transposase_1275475..1275625_NEGATIVE 298 195 157 84 43 NC_002950:PG1177|3end_ISPg1,_transposase_1257794..1257944_NEGATIVE 298 195 157 84 43 NC_002950:PG1031|3end_ISPg1,_transposase_1093081..1093231_NEGATIVE 298 195 157 84 43 NC_002950:PG0825|3end_ISPg1,_transposase_882063..882213_NEGATIVE 298 195 157 84 43 NC_002950:PG0184|3end_ISPg1,_transposase_217567..217717_POSITIVE 291 184 147 126 87 NC_002950:PG2111|3end_thiS_protein_2221282..2221432_NEGATIVE 288 186 143 101 42 NC_002950:PG2158|3end_hypothetical_protein_2264473..2264623_POSITIVE 287 186 144 84 36 NC_002950:PG2194|3end_ISPg4,_transposase_2300962..2301112_NEGATIVE