Origin IGS: aataataagctgaacttaatgtaaccgtatctccggcgaatagcatctcaacacagcggagacaaagcaaacttaatcatttatttccgccttgctgccgacgaaggaagtgtattcatccgttgcacaaggtctattcttcactcctttccgactcgcagaaggcaaaccgccccgaccgaaggaggggatatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatatcccctccttcggtcggggcggtttgccttctgcgagtcggaaaggagtgaagaatagaccttgtgcaacggatgaatacacttccttcgtcggcagcaaggcggaaataaatgattaagtttgctttgtctccgctgtgttgagatgctattcgccggagatacggttacattaagttcagcttattatt Mask Tandem Repeat Region ================================================ aataataagctgaacttaatgtaaccgtatctccggcgaatagcatctcaacacagcggagacaaagcaaacttaatcatttatttccgccttgctgccgacgaaggaagtgtattcatccgttgcacaaggtctattcttcactcctttccgactcgcagaaggcaaaccgccccgaccgaaggaggggatatt Find is-nt database================================================ Query_seq: PG0163:PG0164|PG0163:PG0164:diphosphate--fructose-6-phosphate 1-phosphotransferase:hypothetical protein:<-<-:186976..187170 195 aataataagctgaacttaatgtaaccgtatctccggcgaatagcatctcaacacagcggagacaaagcaaacttaatcatttatttccgccttgctgccgacgaaggaagtgtattcatccgttgcacaaggtctattcttcactcctttccgactcgcagaaggcaaaccgccccgaccgaaggaggggatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0163:PG0164|PG0163:PG0164:diphosphate--fructose-6-phosphate 1-phosphotransferase:hypothetical protein:<-<-:186976..187170 195 aataataagctgaacttaatgtaaccgtatctccggcgaatagcatctcaacacagcggagacaaagcaaacttaatcatttatttccgccttgctgccgacgaaggaagtgtattcatccgttgcacaaggtctattcttcactcctttccgactcgcagaaggcaaaccgccccgaccgaaggaggggatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0163:PG0164|PG0163:PG0164:diphosphate--fructose-6-phosphate 1-phosphotransferase:hypothetical protein:<-<-:186976..187170 195 aataataagctgaacttaatgtaaccgtatctccggcgaatagcatctcaacacagcggagacaaagcaaacttaatcatttatttccgccttgctgccgacgaaggaagtgtattcatccgttgcacaaggtctattcttcactcctttccgactcgcagaaggcaaaccgccccgaccgaaggaggggatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aataataagctgaacttaatgtaaccgtatctccggcgaatagcatctcaacacagcggagacaaagcaaacttaatcatttatttccgccttgctgccgacgaaggaagtgtattcatccgttgcacaaggtctattcttcactcctttccgactcgcagaaggcaaaccgccccgaccgaaggaggggatatt Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 83 End: 193 .................................................................................. M S A L L P T K E V Y S S V A Q G L F F T P F R L A E G K P P R P K E G I .. Protein_Len: 50 Strand: + Start: 45 End: 194 ............................................ M S T Q R R Q S K L N H L F P P C C R R R K C I H P L H K V Y S S L L S D S Q K A N R P D R R R G Y . Protein_Len: 52 Strand: + Start: 40 End: 195 ....................................... M A S Q H S G D K A N L I I Y F R L A A D E G S V F I R C T R S I L H S F P T R R R Q T A P T E G G D I Protein_Len: 39 Strand: - Start: 76 End: 192 ........................................................................... D N I E A K S G V F S T Y E D T A C P R N K V G K R S A S P L G G R G F S P M ... aataataagctgaacttaatgtaaccgtatctccggcgaatagcatctcaacacagcggagacaaagcaaacttaatcatttatttccgccttgctgccgacgaaggaagtgtattcatccgttgcacaaggtctattcttcactcctttccgactcgcagaaggcaaaccgccccgaccgaaggaggggatatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aataataagctgaacttaatgtaaccgtatctccggcgaatagcatctcaacacagcggagacaaagcaaacttaatcatttatttccgccttgctgccgacgaaggaagtgtattcatccgttgcacaaggtctattcttcactcctttccgactcgcagaaggcaaaccgccccgaccgaaggaggggatatt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0163:PG0164|PG0163:PG0164:diphosphate--fructose-6-phosphate 1-phosphotransferase:hypothetical protein:<-<-:186976..187170 195 aataataagctgaacttaatgtaaccgtatctccggcgaatagcatctcaacacagcggagacaaagcaaacttaatcatttatttccgccttgctgccgacgaaggaagtgtattcatccgttgcacaaggtctattcttcactcctttccgactcgcagaaggcaaaccgccccgaccgaaggaggggatatt Intra-Species Hit: Count: 1 Min: 1 Max: 195 Len: 195 Subject: NC_002950_PG0163_PG0164|diphosphate--fructose-6-phosphate 1-phosphotransferase:hypothetical protein|NEGATIVE:NEGATIVE|[186976,187170]|195 HSP 1 e-value: 1.0E-106 bit: 387.0 Len: 195 Query Start:1 Query End:195 Subject Strand: POSITIVE Subject Start: 1 Subject End: 195 aataataagctgaacttaatgtaaccgtatctccggcgaatagcatctcaacacagcggagacaaagcaaacttaatcatttatttccgccttgctgccgacgaaggaagtgtattcatccgttgcacaaggtctattcttcactcctttccgactcgcagaaggcaaaccgccccgaccgaaggaggggatatt aataataagctgaacttaatgtaaccgtatctccggcgaatagcatctcaacacagcggagacaaagcaaacttaatcatttatttccgccttgctgccgacgaaggaagtgtattcatccgttgcacaaggtctattcttcactcctttccgactcgcagaaggcaaaccgccccgaccgaaggaggggatatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAAUAAGCUGAACUUAAUGUAACCGUAUCUCCGGCGAAUAGCAUCUCAACACAGCGGAGACAAAGCAAACUUAAUCAUUUAUUUCCGCCUUGCUGCCGACGAAGGAAGUGUAUUCAUCCGUUGCACAAGGUCUAUUCUUCACUCCUUUCCGACUCGCAGAAGGCAAACCGCCCCGACCGAAGGAGGGGAUAUU .(((.((((.....)))).)))..(((......))).((((((............(((((((.(((.............))).)))))))((((.(((.((((...(((.....)))...)))))))))))..)))))).....((((((((...(((.....(((.....))).....))).)))))))).... (-45.22) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAUCCCCUCCUUCGGUCGGGGCGGUUUGCCUUCUGCGAGUCGGAAAGGAGUGAAGAAUAGACCUUGUGCAACGGAUGAAUACACUUCCUUCGUCGGCAGCAAGGCGGAAAUAAAUGAUUAAGUUUGCUUUGUCUCCGCUGUGUUGAGAUGCUAUUCGCCGGAGAUACGGUUACAUUAAGUUCAGCUUAUUAUU .....((((..(....)..))))((..((((.....))))..)).....(((((((.(((.((((.(((.(..(((.(((((((((((....((.(((..((((((((((..(((....)))..))))))))))..))).))....))).)).)))))))))..)))).))))..)))...)))).)))...... (-52.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 992 70 21 161 112 NC_002950:PG0163|5end_diphosphate--fructose-6-phosphate_1-phosphotransferase_186885..187115_NEGATIVE 423 190 144 203 156 NC_002950:PG1285|5end_glucosamine-6-phosphate_deaminase_1362894..1363124_POSITIVE 409 190 144 50 2 NC_002950:PG0985|5end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_1046930..1047160_POSITIVE 391 126 84 98 51 NC_002950:PG0166|5end_peptidyl-tRNA_hydrolase_188370..188600_NEGATIVE 380 190 151 95 60 NC_002950:PG0145|5end_hypothetical_protein_167831..168061_POSITIVE 372 190 144 204 150 NC_002950:PG0986|5end_hypothetical_protein_1047426..1047656_POSITIVE 364 117 76 141 103 NC_002950:PG1524|5end_2-succinyl-6-hydroxy-2,_4-cyclohexadiene-1-carboxylic_acid_synthase/2-oxoglutarate_decarboxylase_1602746..1602976_NEGATIVE 351 189 150 67 19 NC_002950:PG0538|5end_outer_membrane_efflux_protein_589142..589372_POSITIVE 348 188 141 151 95 NC_002950:PG0858|5end_hypothetical_protein_917540..917770_POSITIVE 347 190 151 224 181 NC_002950:PG2108|5end_thiazole_synthase_2217450..2217680_NEGATIVE 341 180 137 221 163 NC_002950:PG1498|5end_hypothetical_protein_1574656..1574886_POSITIVE 338 190 145 167 120 NC_002950:PG1945|5end_hypothetical_protein_2031697..2031927_POSITIVE 337 190 149 53 9 NC_002950:PG1977|5end_hypothetical_protein_2066693..2066923_NEGATIVE 337 188 143 217 161 NC_002950:PG1853|5end_DNA_polymerase_III,_beta_subunit_1953119..1953349_NEGATIVE 337 188 141 154 96 NC_002950:PG1333|5end_hypothetical_protein_1415425..1415655_NEGATIVE 336 49 8 202 164 NC_002950:PG0744|5end_RNA_methyltransferase,_TrmH_family_791897..792127_NEGATIVE 334 186 151 226 183 NC_002950:PG0223|5end_exonuclease_257610..257840_NEGATIVE 330 190 147 163 110 NC_002950:PG2021|5end_hypothetical_protein_2114852..2115082_NEGATIVE 330 188 150 85 25 NC_002950:PG1388|5end_hypothetical_protein_1470889..1471119_NEGATIVE 328 68 24 169 111 NC_002950:PG0423|5end_hypothetical_protein_462251..462481_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 449 189 144 50 6 NC_002950:PG1608|3end_methylmalonyl-CoA_decarboxylase,_beta_subunit_1688685..1688835_NEGATIVE 384 190 144 70 16 NC_002950:PG1118|3end_clpB_protein_1192083..1192233_NEGATIVE 382 190 152 53 13 NC_002950:PG0143|3end_hydrolase,_carbon-nitrogen_family_165747..165897_NEGATIVE 364 117 76 128 90 NC_002950:PG1525|3end_isochorismate_synthase,_putative_1602759..1602909_NEGATIVE 362 189 144 132 69 NC_002950:PG1497|3end_DNA-binding_protein,_histone-like_family_1574635..1574785_POSITIVE 352 126 89 72 31 NC_002950:PG1964|3end_bacterial_sugar_transferase_2054396..2054546_POSITIVE 339 190 145 64 9 NC_002950:PG1186|3end_hypothetical_protein_1267703..1267853_NEGATIVE 338 190 145 145 98 NC_002950:PG1944|3end_3-phosphoshikimate_1-carboxyvinyltransferase_2031755..2031905_POSITIVE 336 49 8 117 79 NC_002950:PG0745|3end_lactoylglutathione_lyase,_putative_791982..792132_NEGATIVE 335 187 141 147 98 NC_002950:PG0857|3end_transcriptional_regulator,_putative_917623..917773_POSITIVE 332 190 151 78 24 NC_002950:PG0769|3end_fibronectin_type_III_domain_protein_822555..822705_NEGATIVE 331 187 144 117 71 NC_002950:PG0625|3end_GTP_cyclohydrolase_I_679249..679399_POSITIVE 330 188 150 104 44 NC_002950:PG1389|3end_DNA-binding_protein,_histone-like_family_1470870..1471020_NEGATIVE 330 128 87 58 11 NC_002950:PG0373|3end_hypothetical_protein_405453..405603_POSITIVE 326 189 144 45 1 NC_002950:PG0452|3end_hypothetical_protein_495106..495256_POSITIVE 323 123 88 88 49 NC_002950:PG1540|3end_S-adenosylmethionine:tRNA_ribosyltransferase-isomerase_1620409..1620559_POSITIVE 320 188 144 77 28 NC_002950:PG1007|3end_transcriptional_regulator,_GntR_family_1066518..1066668_NEGATIVE 318 178 131 142 83 NC_002950:PG1105|3end_RNA_polymerase_sigma-54_factor_1175716..1175866_NEGATIVE 316 184 151 90 57 NC_002950:PG0229|3end_hypothetical_protein_265350..265500_POSITIVE 314 117 77 52 16 NC_002950:PG1330|3end_large_conductance_mechanosensitive_channel_protein_1410165..1410315_POSITIVE