Origin IGS: ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ggcctcgggggagggaaagactccttatcttcctgtatctctccatcttcgattcaagagagagagaacaccctcccggacatcccgcttgattgcatcggaatatccgcaggttatattcgttttat Mask Tandem Repeat Region ================================================ ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc Find is-nt database================================================ Query_seq: PG0102:PG0103|PG0102:PG0103:hypothetical protein:hypothetical protein:->->:121302..121429 128 ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0102:PG0103|PG0102:PG0103:hypothetical protein:hypothetical protein:->->:121302..121429 128 ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0102:PG0103|PG0102:PG0103:hypothetical protein:hypothetical protein:->->:121302..121429 128 ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 12 End: 101 ........... M T C G Y S D A I K R D V R E G V L S L L N R R W R D T G R ........................... Protein_Len: 37 Strand: + Start: 17 End: 127 ................ M R I F R C N Q A G C P G G C S L S L E S K M E R Y R K I R S L S L P R G . Protein_Len: 30 Strand: - Start: 3 End: 92 .. F R I Y G A S I G I C D L P I D P L T N E R E Q I S S P S M .................................... ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0102:PG0103|PG0102:PG0103:hypothetical protein:hypothetical protein:->->:121302..121429 128 ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc Intra-Species Hit: Count: 4 Min: 1 Max: 128 Len: 128 Subject: NC_002950_PG2073_PG2074|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[2174465,2174592]|128 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:1 Query End:128 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 128 ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc Subject: NC_002950_PG1669_PG1670|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1755657,1755784]|128 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:1 Query End:128 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 128 ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc Subject: NC_002950_PG1264_PG1265|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1344986,1345113]|128 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:1 Query End:128 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 128 ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc Subject: NC_002950_PG0102_PG0103|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[121302,121429]|128 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:1 Query End:128 Subject Strand: POSITIVE Subject Start: 1 Subject End: 128 ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc ataaaacgaatataacctgcggatattccgatgcaatcaagcgggatgtccgggagggtgttctctctctcttgaatcgaagatggagagatacaggaagataaggagtctttccctcccccgaggcc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAAACGAAUAUAACCUGCGGAUAUUCCGAUGCAAUCAAGCGGGAUGUCCGGGAGGGUGUUCUCUCUCUCUUGAAUCGAAGAUGGAGAGAUACAGGAAGAUAAGGAGUCUUUCCCUCCCCCGAGGCC ...............(((..((((((((((...........))))))))))(((((((.(((((((((.((((......)))).))))))).))..((((((.....)))))))))))))...))).. (-45.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGCCUCGGGGGAGGGAAAGACUCCUUAUCUUCCUGUAUCUCUCCAUCUUCGAUUCAAGAGAGAGAGAACACCCUCCCGGACAUCCCGCUUGAUUGCAUCGGAAUAUCCGCAGGUUAUAUUCGUUUUAU (((((((((((((((.((((.......))))......((((((..((((......))))..))))))...))))))).....(((.((......))...)))....))).)))))............. (-39.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 380 65 28 73 33 NC_002950:PG1495|5end_DNA_topoisomerase_III_1571243..1571473_POSITIVE 362 57 16 204 163 NC_002950:PG2073|5end_hypothetical_protein_2174374..2174604_NEGATIVE 362 57 16 204 163 NC_002950:PG1669|5end_hypothetical_protein_1755566..1755796_NEGATIVE 362 57 16 204 163 NC_002950:PG1264|5end_hypothetical_protein_1344895..1345125_NEGATIVE 361 67 28 206 164 NC_002950:PG0635|5end_ribosomal_protein_L11_methyltransferase_687773..688003_NEGATIVE 360 52 16 228 188 NC_002950:PG1259|5end_anaerobic_ribonucleoside-triphosphate_reductase_activating_protein_1335465..1335695_NEGATIVE 354 88 41 50 8 NC_002950:PG0790|5end_GTP-binding_protein_Obg_842583..842813_NEGATIVE 351 58 18 59 17 NC_002950:PG0281|5end_ABC_transporter,_permease_protein,_putative_316023..316253_NEGATIVE 348 67 26 52 16 NC_002950:PG2188|5end_diaminopimelate_decarboxylase_2295781..2296011_NEGATIVE 348 68 21 81 35 NC_002950:PG0608|5end_hypothetical_protein_662945..663175_POSITIVE 346 79 32 147 103 NC_002950:PG1621|5end_hypothetical_protein_1701431..1701661_NEGATIVE 346 86 41 51 10 NC_002950:PG1249|5end_1-acyl-sn-glycerol-3-phosphate_acetyltransferase,_putative_1327161..1327391_NEGATIVE 346 60 18 209 157 NC_002950:PG1220|5end_erythronate-4-phosphate_dehydrogenase_1296986..1297216_POSITIVE 345 59 17 51 6 NC_002950:PG1887|5end_rhodanese-like_domain_protein_1984565..1984795_POSITIVE 345 88 41 99 41 NC_002950:PG1035|5end_hypothetical_protein_1096875..1097105_POSITIVE 342 88 41 110 59 NC_002950:PG0446|5end_thiF_protein_486599..486829_POSITIVE 341 60 16 199 157 NC_002950:PG0884|5end_hypothetical_protein_946479..946709_POSITIVE 339 60 17 74 25 NC_002950:PG0213|5end_precorrin-3_methylase/precorrin-8X_methylmutase_250464..250694_NEGATIVE 337 78 31 218 161 NC_002950:PG0849|5end_hypothetical_protein_911242..911472_NEGATIVE 336 64 21 81 38 NC_002950:PG0032|5end_beta-mannosidase,_putative_40212..40442_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 378 70 21 64 7 NC_002950:PG0231|3end_hypothetical_protein_267409..267559_POSITIVE 368 89 41 52 1 NC_002950:PG0293|3end_secretion_activator_protein,_putative_328093..328243_NEGATIVE 367 87 42 118 59 NC_002950:PG0173|3end_transcriptional_regulator,_putative_194388..194538_NEGATIVE 362 57 16 75 34 NC_002950:PG2074|3end_hypothetical_protein_2174503..2174653_NEGATIVE 362 57 16 75 34 NC_002950:PG1670|3end_hypothetical_protein_1755695..1755845_NEGATIVE 362 57 16 75 34 NC_002950:PG1265|3end_hypothetical_protein_1345024..1345174_NEGATIVE 362 57 16 75 34 NC_002950:PG0102|3end_hypothetical_protein_121241..121391_POSITIVE 348 68 21 50 4 NC_002950:PG0607|3end_hypothetical_protein_662994..663144_POSITIVE 347 90 41 55 1 NC_002950:PG1553|3end_CobN/magnesium_chelatase_family_protein_1635347..1635497_POSITIVE 345 88 41 121 63 NC_002950:PG1034|3end_ABC_transporter,_ATP-binding_protein_1096977..1097127_POSITIVE 343 68 29 147 111 NC_002950:PG1622|3end_DNA_topoisomerase_IV_subunit_A_1701418..1701568_NEGATIVE 343 99 52 129 82 NC_002950:PG0795|3end_hypothetical_protein_849603..849753_POSITIVE 339 60 19 112 40 NC_002950:PG1954|3end_NAD_dependent_epimerase/reductase-related_protein_2045612..2045762_POSITIVE 336 64 21 51 8 NC_002950:PG0033|3end_RmuC_domain_protein_40242..40392_NEGATIVE 335 88 41 144 104 NC_002950:PG1055|3end_thiol_protease_1121202..1121352_NEGATIVE 334 88 41 53 11 NC_002950:PG0844|3end_hypothetical_protein_905461..905611_POSITIVE 333 57 18 68 25 NC_002950:PG2098|3end_hypothetical_protein_2206459..2206609_NEGATIVE 332 58 19 150 96 NC_002950:PG0897|3end_alpha-amylase_family_protein_960868..961018_NEGATIVE 331 90 41 98 35 NC_002950:PG0515|3end_hypothetical_protein_556363..556513_NEGATIVE 328 49 7 148 114 NC_002950:PG0195|3end_rubrerythrin_232704..232854_POSITIVE