Origin IGS: gctcaataaattgcgctgcaaaagtatttatttaattttgcatattatgcttttaaggctctttctttgcactaaatcttattcgatttagaattggaagggtggctaagcaaaatgcaattaagctaaaacattgagaaaagccctgaaagcatgatagccggaagggtactatgtaacggatattccgaatttataa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttataaattcggaatatccgttacatagtacccttccggctatcatgctttcagggcttttctcaatgttttagcttaattgcattttgcttagccacccttccaattctaaatcgaataagatttagtgcaaagaaagagccttaaaagcataatatgcaaaattaaataaatacttttgcagcgcaatttattgagc Mask Tandem Repeat Region ================================================ gctcaataaattgcgctgcaaaagtatttatttaattttgcatattatgcttttaaggctctttctttgcactaaatcttattcgatttagaattggaagggtggctaagcaaaatgcaattaagctaaaacattgagaaaagccctgaaagcatgatagccggaagggtactatgtaacggatattccgaatttataa Find is-nt database================================================ Query_seq: PG0087:PG0088|PG0087:PG0088:SIS domain protein:peptidase, M16 family:<-->:103216..103414 199 gctcaataaattgcgctgcaaaagtatttatttaattttgcatattatgcttttaaggctctttctttgcactaaatcttattcgatttagaattggaagggtggctaagcaaaatgcaattaagctaaaacattgagaaaagccctgaaagcatgatagccggaagggtactatgtaacggatattccgaatttataa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0087:PG0088|PG0087:PG0088:SIS domain protein:peptidase, M16 family:<-->:103216..103414 199 gctcaataaattgcgctgcaaaagtatttatttaattttgcatattatgcttttaaggctctttctttgcactaaatcttattcgatttagaattggaagggtggctaagcaaaatgcaattaagctaaaacattgagaaaagccctgaaagcatgatagccggaagggtactatgtaacggatattccgaatttataa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0087:PG0088|PG0087:PG0088:SIS domain protein:peptidase, M16 family:<-->:103216..103414 199 gctcaataaattgcgctgcaaaagtatttatttaattttgcatattatgcttttaaggctctttctttgcactaaatcttattcgatttagaattggaagggtggctaagcaaaatgcaattaagctaaaacattgagaaaagccctgaaagcatgatagccggaagggtactatgtaacggatattccgaatttataa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gctcaataaattgcgctgcaaaagtatttatttaattttgcatattatgcttttaaggctctttctttgcactaaatcttattcgatttagaattggaagggtggctaagcaaaatgcaattaagctaaaacattgagaaaagccctgaaagcatgatagccggaagggtactatgtaacggatattccgaatttataa Predict ORF larger than 30AA ================================================ Protein_Len: 46 Strand: - Start: 56 End: 193 ....................................................... P E K K A S F R I R N L I P L T A L C F A I L S F C Q S F G Q F C S L R F P V I Y R I N R M ...... gctcaataaattgcgctgcaaaagtatttatttaattttgcatattatgcttttaaggctctttctttgcactaaatcttattcgatttagaattggaagggtggctaagcaaaatgcaattaagctaaaacattgagaaaagccctgaaagcatgatagccggaagggtactatgtaacggatattccgaatttataa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 65 End: 94 ................................................................CTTTGCACTAAATCTTATTCGATTTAGAAT......................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gctcaataaattgcgctgcaaaagtatttatttaattttgcatattatgcttttaaggctctttctttgcactaaatcttattcgatttagaattggaagggtggctaagcaaaatgcaattaagctaaaacattgagaaaagccctgaaagcatgatagccggaagggtactatgtaacggatattccgaatttataa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0087:PG0088|PG0087:PG0088:SIS domain protein:peptidase, M16 family:<-->:103216..103414 199 gctcaataaattgcgctgcaaaagtatttatttaattttgcatattatgcttttaaggctctttctttgcactaaatcttattcgatttagaattggaagggtggctaagcaaaatgcaattaagctaaaacattgagaaaagccctgaaagcatgatagccggaagggtactatgtaacggatattccgaatttataa Intra-Species Hit: Count: 1 Min: 1 Max: 199 Len: 199 Subject: NC_002950_PG0087_PG0088|SIS domain protein:peptidase, M16 family|NEGATIVE:POSITIVE|[103216,103414]|199 HSP 1 e-value: 1.0E-109 bit: 394.0 Len: 199 Query Start:1 Query End:199 Subject Strand: POSITIVE Subject Start: 1 Subject End: 199 gctcaataaattgcgctgcaaaagtatttatttaattttgcatattatgcttttaaggctctttctttgcactaaatcttattcgatttagaattggaagggtggctaagcaaaatgcaattaagctaaaacattgagaaaagccctgaaagcatgatagccggaagggtactatgtaacggatattccgaatttataa gctcaataaattgcgctgcaaaagtatttatttaattttgcatattatgcttttaaggctctttctttgcactaaatcttattcgatttagaattggaagggtggctaagcaaaatgcaattaagctaaaacattgagaaaagccctgaaagcatgatagccggaagggtactatgtaacggatattccgaatttataa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCUCAAUAAAUUGCGCUGCAAAAGUAUUUAUUUAAUUUUGCAUAUUAUGCUUUUAAGGCUCUUUCUUUGCACUAAAUCUUAUUCGAUUUAGAAUUGGAAGGGUGGCUAAGCAAAAUGCAAUUAAGCUAAAACAUUGAGAAAAGCCCUGAAAGCAUGAUAGCCGGAAGGGUACUAUGUAACGGAUAUUCCGAAUUUAUAA ((.(((....))).)).((((((.((......)).)))))).(((((((((((((.((((.((((((....(((((((......)))))))........(..(((((..((.....)).....)))))...)...)))))))))).))))))))))))).((....))...........(((.....)))......... (-52.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAUAAAUUCGGAAUAUCCGUUACAUAGUACCCUUCCGGCUAUCAUGCUUUCAGGGCUUUUCUCAAUGUUUUAGCUUAAUUGCAUUUUGCUUAGCCACCCUUCCAAUUCUAAAUCGAAUAAGAUUUAGUGCAAAGAAAGAGCCUUAAAAGCAUAAUAUGCAAAAUUAAAUAAAUACUUUUGCAGCGCAAUUUAUUGAGC .........(((.....)))..................((....(((((((.(((((((((.((..(((....(((.....((.....))..))).............(((((((......))))))).)))..))))))))))).)))))))....(((((((.((......)).)))))))..))............ (-40.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 825 140 91 231 182 NC_002950:PG0087|5end_SIS_domain_protein_103125..103355_NEGATIVE 332 190 144 179 125 NC_002950:PG0221|5end_hypothetical_protein_255834..256064_NEGATIVE 312 190 147 60 16 NC_002950:PG2116|5end_hypothetical_protein_2224635..2224865_POSITIVE 309 190 143 213 162 NC_002950:PG2207|5end_hypothetical_protein_2320161..2320391_NEGATIVE 309 190 141 95 25 NC_002950:PG1576|5end_L-aspartate_oxidase_1653016..1653246_POSITIVE 305 177 132 93 38 NC_002950:PG1684|5end_hypothetical_protein_1770927..1771157_POSITIVE 296 190 143 142 85 NC_002950:PG1174|5end_thioesterase_family_protein_1253707..1253937_NEGATIVE 294 190 143 48 3 NC_002950:PG2106|5end_hypothetical_protein_2214694..2214924_POSITIVE 287 190 143 191 139 NC_002950:PG1725|5end_competence/damage-inducible_protein_CinA_domain_protein_1814766..1814996_POSITIVE 287 190 141 76 24 NC_002950:PG0133|5end_hypothetical_protein_155764..155994_NEGATIVE 281 185 143 61 19 NC_002950:PG1003|5end_hypothetical_protein_1059232..1059462_POSITIVE 277 189 141 194 149 NC_002950:PG2143|5end_GTP-binding_protein_EngA_2248547..2248777_POSITIVE 276 58 11 71 27 NC_002950:PG0855|5end_hypothetical_protein_916055..916285_NEGATIVE 271 190 143 218 170 NC_002950:PG1663|5end_ABC_transporter,_ATP-binding_protein_1745129..1745359_POSITIVE 270 190 142 216 156 NC_002950:PG0483|5end_kinase,_putative_523844..524074_NEGATIVE 268 176 142 230 198 NC_002950:PG1185|5end_hypothetical_protein_1267752..1267982_NEGATIVE 266 184 142 179 134 NC_002950:PG1113|5end_integrase_1186746..1186976_NEGATIVE 266 171 142 68 36 NC_002950:PG0707|5end_TonB-dependent_receptor,_putative_763632..763862_NEGATIVE 265 171 131 58 9 NC_002950:PG1751|5end_aminotransferase,_class_V_1837280..1837510_POSITIVE 264 190 141 202 152 NC_002950:PG0883|5end_hypothetical_protein_945121..945351_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 332 190 144 100 46 NC_002950:PG0222|3end_DNA-binding_protein,_histone-like_family_255913..256063_NEGATIVE 330 190 144 47 1 NC_002950:PG0046|3end_phosphatidate_cytidylyltransferase_56346..56496_NEGATIVE 305 177 132 96 41 NC_002950:PG1683|3end_hypothetical_protein_1771010..1771160_POSITIVE 287 190 141 57 5 NC_002950:PG0134|3end_magnesium_transporter_155783..155933_NEGATIVE 283 190 141 125 74 NC_002950:PG2046|3end_hypothetical_protein_2145793..2145943_POSITIVE 282 189 141 50 2 NC_002950:PG1501|3end_transcriptional_regulator,_tetR_family_1578500..1578650_NEGATIVE 278 170 124 138 75 NC_002950:PG1672|3end_hypothetical_protein_1757884..1758034_POSITIVE 278 170 124 138 75 NC_002950:PG1267|3end_hypothetical_protein_1347213..1347363_POSITIVE 278 170 124 138 75 NC_002950:PG0100|3end_hypothetical_protein_119052..119202_NEGATIVE 277 189 141 131 86 NC_002950:PG2142|3end_GTP-binding_protein_Era_2248564..2248714_POSITIVE 269 107 61 71 11 NC_002950:PG1136|3end_hypothetical_protein_1216699..1216849_NEGATIVE 265 183 143 64 27 NC_002950:PG0378|3end_elongation_factor_Ts_408808..408958_POSITIVE 264 190 142 100 52 NC_002950:PG2010|3end_phosphomannomutase,_putative_2100952..2101102_POSITIVE 263 170 133 71 30 NC_002950:PG1678|3end_hypothetical_protein_1763755..1763905_NEGATIVE 261 190 142 67 17 NC_002950:PG0147|3end_hypothetical_protein_169128..169278_NEGATIVE 261 183 144 123 74 NC_002950:PG0143|3end_hydrolase,_carbon-nitrogen_family_165747..165897_NEGATIVE 258 190 143 128 81 NC_002950:PG1389|3end_DNA-binding_protein,_histone-like_family_1470870..1471020_NEGATIVE 258 183 143 104 63 NC_002950:PG0606|3end_hypothetical_protein_662451..662601_NEGATIVE 255 190 141 79 24 NC_002950:PG1789|3end_peptidyl-dipeptidase_Dcp_1877417..1877567_NEGATIVE 249 190 143 127 67 NC_002950:PG0164|3end_hypothetical_protein_187081..187231_NEGATIVE