Origin IGS: accatccgaacaaatcttgtcggagtacggcatcttttttcaccgcaaaccatcccttttctccctacgctctcaggcaataccttttggtacgagattgaagattatgccgaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacggtgctcattacaacatgctaattaccagtacattatggacaattaaagtaaaacgagaacgaccttggaaattttcccaatttcgcgtcttcaaaattcattttccccccttgtttgggaagatcaggactcaatagcagatcgatgacctcgactctccttgtcgtatttgaaaggtcgtactccgtatccttggtctcttccttctacaattaggatctctaacacattaattcgtgcttattccggacagaacatcatcaggcttatatgtctatgcgattgccctgactgttatatttcttcacggtcttttccttacttttgtttccgttccacgaaagcgttatccaagcgtgtaagatctgcacaga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tctgtgcagatcttacacgcttggataacgctttcgtggaacggaaacaaaagtaaggaaaagaccgtgaagaaatataacagtcagggcaatcgcatagacatataagcctgatgatgttctgtccggaataagcacgaattaatgtgttagagatcctaattgtagaaggaagagaccaaggatacggagtacgacctttcaaatacgacaaggagagtcgaggtcatcgatctgctattgagtcctgatcttcccaaacaaggggggaaaatgaattttgaagacgcgaaattgggaaaatttccaaggtcgttctcgttttactttaattgtccataatgtactggtaattagcatgttgtaatgagcaccgtacgcctacgctggtctcctgcatcgtgcaggagtgcatcgtacgcctacgatcgtaagtgtttcgtaccggcttttcaacacttcttcgtattcctcggcataatcttcaatctcgtaccaaaaggtattgcctgagagcgtagggagaaaagggatggtttgcggtgaaaaaagatgccgtactccgacaagatttgttcggatggt Mask Tandem Repeat Region ================================================ accatccgaacaaatcttgtcggagtacggcatcttttttcaccgcaaaccatcccttttctccctacgctctcaggcaataccttttggtacgagattgaagattatgccgaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacggtgctcattacaacatgctaattaccagtacattatggacaattaaagtaaaacgagaacgaccttggaaattttcccaatttcgcgtcttcaaaattcattttccccccttgtttgggaagatcaggactcaatagcagatcgatgacctcgactctccttgtcgtatttgaaaggtcgtactccgtatccttggtctcttccttctacaattaggatctctaacacattaattcgtgcttattccggacagaacatcatcaggcttatatgtctatgcgattgccctgactgttatatttcttcacggtcttttccttacttttgtttccgttccacgaaagcgttatccaagcgtgtaagatctgcacaga Find is-nt database================================================ Query_seq: PG0013:PG0016|PG0013:PG0016:hypothetical protein:sigma-54 dependent DNA-binding response regulator:->->:16502..17086 585 accatccgaacaaatcttgtcggagtacggcatcttttttcaccgcaaaccatcccttttctccctacgctctcaggcaataccttttggtacgagattgaagattatgccgaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacggtgctcattacaacatgctaattaccagtacattatggacaattaaagtaaaacgagaacgaccttggaaattttcccaatttcgcgtcttcaaaattcattttccccccttgtttgggaagatcaggactcaatagcagatcgatgacctcgactctccttgtcgtatttgaaaggtcgtactccgtatccttggtctcttccttctacaattaggatctctaacacattaattcgtgcttattccggacagaacatcatcaggcttatatgtctatgcgattgccctgactgttatatttcttcacggtcttttccttacttttgtttccgttccacgaaagcgttatccaagcgtgtaagatctgcacaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PG0013:PG0016|PG0013:PG0016:hypothetical protein:sigma-54 dependent DNA-binding response regulator:->->:16502..17086 585 accatccgaacaaatcttgtcggagtacggcatcttttttcaccgcaaaccatcccttttctccctacgctctcaggcaataccttttggtacgagattgaagattatgccgaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacggtgctcattacaacatgctaattaccagtacattatggacaattaaagtaaaacgagaacgaccttggaaattttcccaatttcgcgtcttcaaaattcattttccccccttgtttgggaagatcaggactcaatagcagatcgatgacctcgactctccttgtcgtatttgaaaggtcgtactccgtatccttggtctcttccttctacaattaggatctctaacacattaattcgtgcttattccggacagaacatcatcaggcttatatgtctatgcgattgccctgactgttatatttcttcacggtcttttccttacttttgtttccgttccacgaaagcgttatccaagcgtgtaagatctgcacaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PG0013:PG0016|PG0013:PG0016:hypothetical protein:sigma-54 dependent DNA-binding response regulator:->->:16502..17086 585 accatccgaacaaatcttgtcggagtacggcatcttttttcaccgcaaaccatcccttttctccctacgctctcaggcaataccttttggtacgagattgaagattatgccgaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacggtgctcattacaacatgctaattaccagtacattatggacaattaaagtaaaacgagaacgaccttggaaattttcccaatttcgcgtcttcaaaattcattttccccccttgtttgggaagatcaggactcaatagcagatcgatgacctcgactctccttgtcgtatttgaaaggtcgtactccgtatccttggtctcttccttctacaattaggatctctaacacattaattcgtgcttattccggacagaacatcatcaggcttatatgtctatgcgattgccctgactgttatatttcttcacggtcttttccttacttttgtttccgttccacgaaagcgttatccaagcgtgtaagatctgcacaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 accatccgaacaaatcttgtcggagtacggcatcttttttcaccgcaaaccatcccttttctccctacgctctcaggcaataccttttggtacgagattgaagattatgccgaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacggtgctcattacaacatgctaattaccagtacattatggacaattaaagtaaaacgagaacgaccttggaaattttcccaatttcgcgtcttcaaaattcattttccccccttgtttgggaagatcaggactcaatagcagatcgatgacctcgactctccttgtcgtatttgaaaggtcgtactccgtatccttggtctcttccttctacaattaggatctctaacacattaattcgtgcttattccggacagaacatcatcaggcttatatgtctatgcgattgccctgactgttatatttcttcacggtcttttccttacttttgtttccgttccacgaaagcgttatccaagcgtgtaagatctgcacaga Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 14 End: 130 ............. M L S E Y G I F F H R K P S L F S L R S Q A I P F G T R L K I M P R N T K K C ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 4 End: 201 ... M R T N L V G V R H L F S P Q T I P F L P T L S G N T F W Y E I E D Y A E E Y E E V L K S R Y E T L T I V G V R C T P A ................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 46 Strand: + Start: 117 End: 254 .................................................................................................................... M R R S V E K P V R N T Y D R R R T M H S C T M Q E T S V G V R C S L Q H A N Y Q Y I M D N ........................................................................................................................................................................................................................................................................................................................................... Protein_Len: 40 Strand: + Start: 226 End: 345 ................................................................................................................................................................................................................................. M L I T S T L W T I K V K R E R P W K F S Q F R V F K I H F P P L F G K I R T Q ................................................................................................................................................................................................................................................ Protein_Len: 38 Strand: + Start: 311 End: 424 ...................................................................................................................................................................................................................................................................................................................... M F P P C L G R S G L N S R S M T S T L L V V F E R S Y S V S L V S S F Y N ................................................................................................................................................................. Protein_Len: 41 Strand: + Start: 448 End: 570 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................... M L I P D R T S S G L Y V Y A I A L T V I F L H G L F L T F V S V P R K R Y P S V ............... Protein_Len: 60 Strand: + Start: 372 End: 584 ................................................................................................................................................................................................................................................................................................................................................................................... M S Y L K G R T P Y P W S L P S T I R I S N T L I R A Y S G Q N I I R L I C L C D C P D C Y I S S R S F P Y F C F R S T . Protein_Len: 34 Strand: - Start: 481 End: 582 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ I D I R N G Q S N Y K K V T K E K S K N G N W S L T I W A H L I Q M ... Protein_Len: 36 Strand: - Start: 338 End: 445 ................................................................................................................................................................................................................................................................................................................................................. S E I A S R H G R S E K D Y K F P R V G Y G Q D R G E V I L I E L V N M ............................................................................................................................................ Protein_Len: 37 Strand: - Start: 247 End: 357 ...................................................................................................................................................................................................................................................... P C N F Y F S F S R P F K G L K A D E F N M K G G Q K P L D P S L L L D M .................................................................................................................................................................................................................................... Protein_Len: 48 Strand: - Start: 76 End: 219 ........................................................................... A I G K P V L N F I I G L F V F F H Q F A P V F C K R D Y A Y S A S R C S A P S W R L R V T S M .............................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 32 Strand: - Start: 3 End: 98 .. M R V F R T P T R C R K E G C V M G K R G V S E P L V K Q Y S M ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... accatccgaacaaatcttgtcggagtacggcatcttttttcaccgcaaaccatcccttttctccctacgctctcaggcaataccttttggtacgagattgaagattatgccgaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacggtgctcattacaacatgctaattaccagtacattatggacaattaaagtaaaacgagaacgaccttggaaattttcccaatttcgcgtcttcaaaattcattttccccccttgtttgggaagatcaggactcaatagcagatcgatgacctcgactctccttgtcgtatttgaaaggtcgtactccgtatccttggtctcttccttctacaattaggatctctaacacattaattcgtgcttattccggacagaacatcatcaggcttatatgtctatgcgattgccctgactgttatatttcttcacggtcttttccttacttttgtttccgttccacgaaagcgttatccaagcgtgtaagatctgcacaga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ accatccgaacaaatcttgtcggagtacggcatcttttttcaccgcaaaccatcccttttctccctacgctctcaggcaataccttttggtacgagattgaagattatgccgaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacggtgctcattacaacatgctaattaccagtacattatggacaattaaagtaaaacgagaacgaccttggaaattttcccaatttcgcgtcttcaaaattcattttccccccttgtttgggaagatcaggactcaatagcagatcgatgacctcgactctccttgtcgtatttgaaaggtcgtactccgtatccttggtctcttccttctacaattaggatctctaacacattaattcgtgcttattccggacagaacatcatcaggcttatatgtctatgcgattgccctgactgttatatttcttcacggtcttttccttacttttgtttccgttccacgaaagcgttatccaagcgtgtaagatctgcacaga Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PG0013:PG0016|PG0013:PG0016:hypothetical protein:sigma-54 dependent DNA-binding response regulator:->->:16502..17086 585 accatccgaacaaatcttgtcggagtacggcatcttttttcaccgcaaaccatcccttttctccctacgctctcaggcaataccttttggtacgagattgaagattatgccgaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacggtgctcattacaacatgctaattaccagtacattatggacaattaaagtaaaacgagaacgaccttggaaattttcccaatttcgcgtcttcaaaattcattttccccccttgtttgggaagatcaggactcaatagcagatcgatgacctcgactctccttgtcgtatttgaaaggtcgtactccgtatccttggtctcttccttctacaattaggatctctaacacattaattcgtgcttattccggacagaacatcatcaggcttatatgtctatgcgattgccctgactgttatatttcttcacggtcttttccttacttttgtttccgttccacgaaagcgttatccaagcgtgtaagatctgcacaga Intra-Species Hit: Count: 6 Min: 1 Max: 585 Len: 585 Subject: NC_002950_PG0013_PG0016|hypothetical protein:sigma-54 dependent DNA-binding response regulator|POSITIVE:POSITIVE|[16502,17086]|585 HSP 1 e-value: 0.0 bit: 1160.0 Len: 585 Query Start:1 Query End:585 Subject Strand: POSITIVE Subject Start: 1 Subject End: 585 accatccgaacaaatcttgtcggagtacggcatcttttttcaccgcaaaccatcccttttctccctacgctctcaggcaataccttttggtacgagattgaagattatgccgaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacggtgctcattacaacatgctaattaccagtacattatggacaattaaagtaaaacgagaacgaccttggaaattttcccaatttcgcgtcttcaaaattcattttccccccttgtttgggaagatcaggactcaatagcagatcgatgacctcgactctccttgtcgtatttgaaaggtcgtactccgtatccttggtctcttccttctacaattaggatctctaacacattaattcgtgcttattccggacagaacatcatcaggcttatatgtctatgcgattgccctgactgttatatttcttcacggtcttttccttacttttgtttccgttccacgaaagcgttatccaagcgtgtaagatctgcacaga accatccgaacaaatcttgtcggagtacggcatcttttttcaccgcaaaccatcccttttctccctacgctctcaggcaataccttttggtacgagattgaagattatgccgaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacggtgctcattacaacatgctaattaccagtacattatggacaattaaagtaaaacgagaacgaccttggaaattttcccaatttcgcgtcttcaaaattcattttccccccttgtttgggaagatcaggactcaatagcagatcgatgacctcgactctccttgtcgtatttgaaaggtcgtactccgtatccttggtctcttccttctacaattaggatctctaacacattaattcgtgcttattccggacagaacatcatcaggcttatatgtctatgcgattgccctgactgttatatttcttcacggtcttttccttacttttgtttccgttccacgaaagcgttatccaagcgtgtaagatctgcacaga Subject: NC_002950_PG1566_PG1570|glutamyl-tRNA synthetase:rhodanese-like domain protein|NEGATIVE:NEGATIVE|[1647492,1648671]|1180 HSP 1 e-value: 1.0E-102 bit: 373.0 Len: 280 Query Start:65 Query End:344 Subject Strand: POSITIVE Subject Start: 445 Subject End: 723 ................................................................ctacgctctcaggcaataccttttggtacgagattgaagattatgccgaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacggtgctcattacaacatgctaattaccagtacattatggacaattaaagtaaaacgagaacgaccttggaaattttcccaatttcgcgtcttcaaaattcattttccccccttgtttgggaagatcaggactca................................................................................................................................................................................................................................................. ................................................................ctacgctctcaggtgatacctct-ggtacgagattgaagattatgcagaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgctctcctgcacggtgcaggagaccggcgtaggcgtacggtgctcgttacaacatgctaattaccagtacattatggccaattaaggtgaaatgaaaataatcttggaagttttccctgttttgcgtcttcaaaattcatttttcccccttgtttgggaagatcaggactca................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-43 bit: 176.0 Len: 101 Query Start:375 Query End:475 Subject Strand: POSITIVE Subject Start: 828 Subject End: 928 ......................................................................................................................................................................................................................................................................................................................................................................................tcgtatttgaaaggtcgtactccgtatccttggtctcttccttctacaattaggatctctaacacattaattcgtgcttattccggacagaacatcatcag.............................................................................................................. ......................................................................................................................................................................................................................................................................................................................................................................................tcgtatttgaaaggttgtactccgtatccttggtctcttccttctacaattaggatctctaacacattaattcgtgtttattccggacggaacatcatcag.............................................................................................................. Subject: NC_002950_PG1186_PG1189|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[1269242,1270131]|890 HSP 1 e-value: 1.0E-102 bit: 373.0 Len: 280 Query Start:65 Query End:344 Subject Strand: NEGATIVE Subject Start: 278 Subject End: 557 ................................................................ctacgctctcaggcaataccttttggtacgagattgaagattatgccgaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacggtgctcattacaacatgctaattaccagtacattatggacaattaaagtaaaacgagaacgaccttggaaattttcccaatttcgcgtcttcaaaattcattttccccccttgtttgggaagatcaggactca................................................................................................................................................................................................................................................. ................................................................ctacgctctcaggtgatacctcttggtacgagattgaagattatgcagaggaatacgaagaagtgttgaaaagccggtacgaaacacttacgatcgtaggcgtacgatgccttcctgcacggtgcaggagaccggcgtaggcctacggtgctcattgcaacatgctaattaccagtacattatggccaattaaggtgaaatgaaaataatcttggaagttttccctgttttgcgtcttcaaaattcatttttcccccttgtttgggaagatcaggactca................................................................................................................................................................................................................................................. HSP 2 e-value: 1.0E-40 bit: 168.0 Len: 109 Query Start:375 Query End:483 Subject Strand: NEGATIVE Subject Start: 65 Subject End: 173 ......................................................................................................................................................................................................................................................................................................................................................................................tcgtatttgaaaggtcgtactccgtatccttggtctcttccttctacaattaggatctctaacacattaattcgtgcttattccggacagaacatcatcaggcttatat...................................................................................................... ......................................................................................................................................................................................................................................................................................................................................................................................tcgtatttgaaaggttgtactctgtatccttggtctcttccttctactattaggatctctaacacattaattcgtgtttattccggacggagcatcatcaggcttatat...................................................................................................... Subject: NC_002950_PG1335_PG1337|hypothetical protein:umuD protein|NEGATIVE:POSITIVE|[1416985,1417952]|968 HSP 1 e-value: 1.0E-80 bit: 301.0 Len: 232 Query Start:154 Query End:385 Subject Strand: NEGATIVE Subject Start: 155 Subject End: 384 .........................................................................................................................................................acgatcgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacggtgctcattacaacatgctaattaccagtacattatggacaattaaagtaaaacgagaacgaccttggaaattttcccaatttcgcgtcttcaaaattcattttccccccttgtttgggaagatcaggactcaatagcagatcgatgacctcgactctccttgtcgtatttgaa........................................................................................................................................................................................................ .........................................................................................................................................................acgatcgtaggcgtacgacgccttcctgcacggtgcaggagaccagcgtaggcctacggtgctcattacaacatgctaattatcagtgcattatggccaattaaagtaaaacgagaacgaccttagaaattttccctgttttgcgtcttcaaaattc-gtttccccccttgtttgggaagttca-gactcaatagcagatcgacgacctcgactccccttgtcgtatttgaa........................................................................................................................................................................................................ Subject: NC_002950_PG0759_PG0762|TPR domain protein:trigger factor, putative|NEGATIVE:POSITIVE|[810876,812898]|2023 HSP 1 e-value: 1.0E-46 bit: 188.0 Len: 223 Query Start:159 Query End:381 Subject Strand: NEGATIVE Subject Start: 1459 Subject End: 1679 ..............................................................................................................................................................cgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacggtgctcattacaacatgctaattaccagtacattatggacaattaaagtaaaacgagaacgaccttggaaattttcccaatttcgcgtcttcaaaattcattttccccccttgtttgggaagatcaggactcaatagcagatcgatgacctcgactctccttgtcgtatt............................................................................................................................................................................................................ ..............................................................................................................................................................cgtaggcgtacgaaaccttcctgcacggtgcaggagaccagcgtaggcctacagtactaattacaacatgctaattaccagtacattatagccaattaaggtgaaatgaaaataatcttggaagttttccctgttttgcgtcttcaaaattcattttttccccttgtttgggaagct-ttgactcaatagcagatcgacgacctcgactc-ccttgtcgtatt............................................................................................................................................................................................................ HSP 2 e-value: 3.0E-17 bit: 91.7 Len: 58 Query Start:154 Query End:211 Subject Strand: NEGATIVE Subject Start: 1668 Subject End: 1725 .........................................................................................................................................................acgatcgtaggcgtacgatgcactcctgcacgatgcaggagaccagcgtaggcgtacg...................................................................................................................................................................................................................................................................................................................................................................................... .........................................................................................................................................................acgatcgtagacgtacgatactctcctgcacgatgcaggagaccagcgtaggcgtacg...................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_002950_PG2092_PG2094|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[2193001,2193410]|410 HSP 1 e-value: 5.0E-34 bit: 147.0 Len: 100 Query Start:379 Query End:478 Subject Strand: POSITIVE Subject Start: 221 Subject End: 317 ..........................................................................................................................................................................................................................................................................................................................................................................................atttgaaaggtcgtactccgtatccttggtctcttccttctacaattaggatctctaacacattaattcgtgcttattccggacagaacatcatcaggct........................................................................................................... ..........................................................................................................................................................................................................................................................................................................................................................................................atttgaaaggttgtactccg---ccttggtctcttccttctacaattaggatctctaacacattaattcgtgcttattccggacggagcatcatcaggct........................................................................................................... HSP 2 e-value: 7.0E-18 bit: 93.7 Len: 74 Query Start:312 Query End:385 Subject Strand: POSITIVE Subject Start: 33 Subject End: 106 .......................................................................................................................................................................................................................................................................................................................ttttccccccttgtttgggaagatcag-gactcaatagcagatcgatgacctcgactctccttgtcgtatttgaa........................................................................................................................................................................................................ .......................................................................................................................................................................................................................................................................................................................ttttccccccttgtttgggaagctcggagactcaatagcagattgatgacctcgactc-ccttgtcgtatttgaa........................................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACCAUCCGAACAAAUCUUGUCGGAGUACGGCAUCUUUUUUCACCGCAAACCAUCCCUUUUCUCCCUACGCUCUCAGGCAAUACCUUUUGGUACGAGAUUGAAGAUUAUGCCGAGGAAUACGAAGAAGUGUUGAAAAGCCGGUACGAAACACUUACGAUCGUAGGCGUACGAUGCACUCCUGCACGAUGCAGGAGACCAGCGUAGGCGUACGGUGCUCAUUACAACAUGCUAAUUACCAGUACAUUAUGGACAAUUAAAGUAAAACGAGAACGACCUUGGAAAUUUUCCCAAUUUCGCGUCUUCAAAAUUCAUUUUCCCCCCUUGUUUGGGAAGAUCAGGACUCAAUAGCAGAUCGAUGACCUCGACUCUCCUUGUCGUAUUUGAAAGGUCGUACUCCGUAUCCUUGGUCUCUUCCUUCUACAAUUAGGAUCUCUAACACAUUAAUUCGUGCUUAUUCCGGACAGAACAUCAUCAGGCUUAUAUGUCUAUGCGAUUGCCCUGACUGUUAUAUUUCUUCACGGUCUUUUCCUUACUUUUGUUUCCGUUCCACGAAAGCGUUAUCCAAGCGUGUAAGAUCUGCACAGA ....(((((.((.....)))))))((.(((.(((((....(((.((.............((((.....(((....)))..((((....)))).)))).....(((.((((((.(((((....(((((((..((((((((((((((((....((((.((((((.((.((((.((..(((((((.....)))))))..)).)))).)).))))))......................(((........))).........))))...((((..((((..(((...............(.(((((........((((((((.........)))))))).))))).)....((.((...(((((((((((.......))))........)))))))..)).)).)))..))))...((((.........))))))))...........))))))).(((..(((.(((..((.(((.((((......)))))))....))..))).)))..)))........))).))))))..)))))))......))))).))...)))).)))...))))).)))))))).))... (-149.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUGUGCAGAUCUUACACGCUUGGAUAACGCUUUCGUGGAACGGAAACAAAAGUAAGGAAAAGACCGUGAAGAAAUAUAACAGUCAGGGCAAUCGCAUAGACAUAUAAGCCUGAUGAUGUUCUGUCCGGAAUAAGCACGAAUUAAUGUGUUAGAGAUCCUAAUUGUAGAAGGAAGAGACCAAGGAUACGGAGUACGACCUUUCAAAUACGACAAGGAGAGUCGAGGUCAUCGAUCUGCUAUUGAGUCCUGAUCUUCCCAAACAAGGGGGGAAAAUGAAUUUUGAAGACGCGAAAUUGGGAAAAUUUCCAAGGUCGUUCUCGUUUUACUUUAAUUGUCCAUAAUGUACUGGUAAUUAGCAUGUUGUAAUGAGCACCGUACGCCUACGCUGGUCUCCUGCAUCGUGCAGGAGUGCAUCGUACGCCUACGAUCGUAAGUGUUUCGUACCGGCUUUUCAACACUUCUUCGUAUUCCUCGGCAUAAUCUUCAAUCUCGUACCAAAAGGUAUUGCCUGAGAGCGUAGGGAGAAAAGGGAUGGUUUGCGGUGAAAAAAGAUGCCGUACUCCGACAAGAUUUGUUCGGAUGGU ((((.(((((((((.....(((((.((((((.((((((....(....)................(((.((((((.(((....((((((((....)).............)))))).))))))).)))))......)))))).....))))))....((((.........)))).....)))))....(((((((((...(((((((...(((.((.(((.((((.....))))))).)).....)))....(((((((......)))))))........)))))))..((((..((((((....))))))..))))(((..(((((((..(((..(((........((((((...(((((......((((....((((.((.((((.((..(((((((.....)))))))..)).)))).)).)))).))))..)))))...))))))...........((((((.(((.((((((((..............(((((....))))))))).))).).))).))))))...)))..)))...)))))))..)))...)))))))))..))))))))).)))).... (-176.04) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 720 370 321 183 133 NC_002950:PG2092|5end_hypothetical_protein_2192910..2193140_NEGATIVE 708 480 431 148 99 NC_002950:PG1787|5end_hypothetical_protein_1875908..1876138_NEGATIVE 492 195 154 230 188 NC_002950:PG1974|5end_hypothetical_protein_2063055..2063285_NEGATIVE 459 200 156 231 179 NC_002950:PG1970|5end_hypothetical_protein_2059783..2060013_NEGATIVE 418 197 156 61 19 NC_002950:PG2114|5end_hypothetical_protein_2224158..2224388_NEGATIVE 418 215 172 130 74 NC_002950:PG1605|5end_aminopeptidase_C_1685179..1685409_POSITIVE 415 200 155 197 149 NC_002950:PG1975|5end_hemagglutinin_protein_HagC_2063218..2063448_POSITIVE 404 204 161 55 12 NC_002950:PG1583|5end_batB_protein_1660315..1660545_POSITIVE 400 52 15 56 10 NC_002950:PG1551|5end_hmuY_protein_1628438..1628668_POSITIVE 394 210 163 88 31 NC_002950:PG1172|5end_iron-sulfur_cluster_binding_protein,_putative_1251173..1251403_POSITIVE 389 70 21 197 136 NC_002950:PG0744|5end_RNA_methyltransferase,_TrmH_family_791897..792127_NEGATIVE 387 207 163 151 113 NC_002950:PG2149|5end_hypothetical_protein_2257658..2257888_NEGATIVE 387 208 163 72 16 NC_002950:PG0150|5end_conserved_hypothetical_protein_TIGR01125_173544..173774_NEGATIVE 386 218 180 72 29 NC_002950:PG1667|5end_outer_membrane_efflux_protein_1749545..1749775_POSITIVE 378 209 162 209 161 NC_002950:PG2027|5end_hypothetical_protein_2125494..2125724_POSITIVE 378 217 177 227 183 NC_002950:PG0756|5end_hypothetical_protein_805673..805903_NEGATIVE 378 218 177 212 173 NC_002950:PG0729|5end_D-alanylalanine_synthetase_780818..781048_NEGATIVE 376 207 162 55 3 NC_002950:PG1418|5end_DNA_polymerase_III,_gamma_and_tau_subunits_1503064..1503294_POSITIVE 375 212 171 51 22 NC_002950:PG0575|5end_penicillin-binding_protein_2,_putative_629501..629731_POSITIVE 374 48 2 191 140 NC_002950:PG1449|5end_hypothetical_protein_1534262..1534492_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 404 204 161 44 1 NC_002950:PG1582|3end_batA_protein_1660384..1660534_POSITIVE 395 217 171 137 93 NC_002950:PG1027|3end_hypothetical_protein_1089197..1089347_NEGATIVE 389 70 21 112 51 NC_002950:PG0745|3end_lactoylglutathione_lyase,_putative_791982..792132_NEGATIVE 387 207 163 142 104 NC_002950:PG2150|3end_LysM_domain_protein_2257667..2257817_NEGATIVE 387 208 163 75 19 NC_002950:PG0151|3end_signal_recognition_particle-docking_protein_FtsY_173541..173691_NEGATIVE 386 218 180 75 32 NC_002950:PG1666|3end_efflux_transporter,_MFP_component,_RND_family_1749628..1749778_POSITIVE 375 213 174 83 39 NC_002950:PG1620|3end_carboxyl-terminal_protease-related_protein_1699415..1699565_NEGATIVE 375 212 171 41 12 NC_002950:PG0574|3end_hypothetical_protein_629571..629721_POSITIVE 374 208 166 144 105 NC_002950:PG0010|3end_ATP-dependent_Clp_protease,_ATP-binding_subunit_ClpC_11434..11584_POSITIVE 367 218 173 54 10 NC_002950:PG0747|3end_sigma-54_dependent_DNA-binding_response_regulator_793900..794050_NEGATIVE 363 179 136 146 95 NC_002950:PG0768|3end_hypothetical_protein_821275..821425_NEGATIVE 361 44 2 150 102 NC_002950:PG1450|3end_hypothetical_protein_1534300..1534450_NEGATIVE 361 44 2 150 102 NC_002950:PG0823|3end_hypothetical_protein_881212..881362_POSITIVE 360 580 533 77 20 NC_002950:PG0007|3end_hypothetical_protein_6755..6905_NEGATIVE 356 217 172 63 15 NC_002950:PG1366|3end_UDP-N-acetylglucosamine_1-carboxyvinyltransferase_1446076..1446226_NEGATIVE 354 217 173 113 58 NC_002950:PG1218|3end_hypothetical_protein_1296475..1296625_POSITIVE 350 110 61 69 8 NC_002950:PG1693|3end_HesA/MoeB/ThiF_family_protein_1777170..1777320_NEGATIVE 350 209 162 63 23 NC_002950:PG0076|3end_N-acetylmuramoyl-L-alanine_amidase,_family_4_90392..90542_NEGATIVE 346 207 162 117 77 NC_002950:PG2214|3end_hypothetical_protein_2325301..2325451_NEGATIVE 346 218 171 48 6 NC_002950:PG0605|3end_hypothetical_protein_661165..661315_NEGATIVE